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- EMDB-54211: Structure of the Ypt7 GEF complex Mon1-Ccz1 from Chaetomium Therm... -

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Basic information

Entry
Database: EMDB / ID: EMD-54211
TitleStructure of the Ypt7 GEF complex Mon1-Ccz1 from Chaetomium Thermophilum
Map data
Sample
  • Complex: Complex of the Rab7 GEF Mon1-Ccz1
    • Protein or peptide: Vacuolar fusion protein MON1
    • Protein or peptide: CCZ1/INTU/HSP4 first Longin domain-containing protein
Keywordslongin domain / GEF / nucleotide exchange / endosome / autophagosome / CYTOSOLIC PROTEIN
Function / homology
Function and homology information


Mon1-Ccz1 complex / protein targeting to vacuole / multivesicular body membrane / fungal-type vacuole membrane / vesicle-mediated transport / autophagy
Similarity search - Function
Vacuolar fusion protein Ccz1 / Vacuolar fusion protein Mon1 / CCZ1/INTU/HSP4, first Longin domain / First Longin domain of INTU, CCZ1 and HPS4 / FUZ/MON1/HPS1, third Longin domain / FUZ/MON1/HPS1, second Longin domain / FUZ/MON1/HPS1, first Longin domain / First Longin domain of FUZ, MON1 and HPS1 / Second Longin domain of FUZ, MON1 and HPS1 / Third Longin domain of FUZ, MON1 and HPS1
Similarity search - Domain/homology
CCZ1/INTU/HSP4 first Longin domain-containing protein / Vacuolar fusion protein MON1
Similarity search - Component
Biological speciesThermochaetoides thermophila (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsWilmes S / Schaefer J / Moeller A / Kuemmel D
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)SFB1557-P10 Germany
German Research Foundation (DFG)SFB1557-P11 Germany
CitationJournal: To Be Published
Title: Mechanistic Adaptation of the Metazoan RabGEFs Mon1-Ccz1 and Fuzzy-Inturned
Authors: Wilmes S / Toenjes J / Drechsler M / Ruf A / Schaefer J / Luerick A / Januliene D / Apelt S / Di Iorio D / Wegner SV / Loose M / Moeller A / Paululat A / Kuemmel D
History
DepositionJun 30, 2025-
Header (metadata) releaseJul 30, 2025-
Map releaseJul 30, 2025-
UpdateJul 30, 2025-
Current statusJul 30, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_54211.png

Downloads & links

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Map

FileDownload / File: emd_54211.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.68 Å/pix.
x 440 pix.
= 299.2 Å		0.68 Å/pix.
x 440 pix.
= 299.2 Å		0.68 Å/pix.
x 440 pix.
= 299.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.68 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0595
Minimum - Maximum-0.166298 - 0.29579955
Average (Standard dev.)-0.00017885906 (±0.0050761136)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 299.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_54211_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_54211_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Complex of the Rab7 GEF Mon1-Ccz1

EntireName: Complex of the Rab7 GEF Mon1-Ccz1
Components
  • Complex: Complex of the Rab7 GEF Mon1-Ccz1
    • Protein or peptide: Vacuolar fusion protein MON1
    • Protein or peptide: CCZ1/INTU/HSP4 first Longin domain-containing protein

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Supramolecule #1: Complex of the Rab7 GEF Mon1-Ccz1

SupramoleculeName: Complex of the Rab7 GEF Mon1-Ccz1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Thermochaetoides thermophila (fungus)

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Macromolecule #1: Vacuolar fusion protein MON1

MacromoleculeName: Vacuolar fusion protein MON1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 72.198766 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: GPLGSMTQNN APESVAAEGT VAGQQTDTTK PEPSSQPAPE PPSQVTTDFP PNNKPNDVPA TEPASSSPRP TEPPAPPPPK PTIALSPLD IATLTFPDGT RGTFPAPPQT SAQSVSTPSI ASGHVTPQRD TDTASITSVA GTLRGVTGTA GDLASLLAGD G LGRKSKAW ...String:
GPLGSMTQNN APESVAAEGT VAGQQTDTTK PEPSSQPAPE PPSQVTTDFP PNNKPNDVPA TEPASSSPRP TEPPAPPPPK PTIALSPLD IATLTFPDGT RGTFPAPPQT SAQSVSTPSI ASGHVTPQRD TDTASITSVA GTLRGVTGTA GDLASLLAGD G LGRKSKAW RVLRAQQQAC GEGSGEEGEI TEIEGLGLGE GMEGFERELD NIPDTLPDDE RLALWKGKLK HYLILSSAGK PI WSRHGDL SLVNSTMGVV QTIISFYEGA RNPLLGFTAG KVRFVILIKG PLYFVAISRL RESDAQLRAQ LEALYMQILS TLT LPILTN IFAHRPSTDL RGPLQGTESL LASLADSFTK GSPSTLLSAL ECLRLRKSQR QAITNIFLKS RCEELLYGLL VAGG KLVSV IRPRKHSLHP SDLQLIFNML FESGGIKGNG GENWIPLCLP AFNNTGYLYM YVSFLDDKAP DDQNQPPESS NLDAS NKNS SNTPDDDLTA LILISPSREA FYALQSMRTR LVSQLLSTGY LSLIRSTALS GRPSITSILP KTPLLHFLYK SRPNVQ WCM SSLSSLTPPG ATATETLLAR RKLMSVYEEL HAALHARHAH LRVVYSTADE KEGEGLACLG WSTPAFEVYC VAPGCVG RA GMAREVNRVV QWARREEERL FILGGGVF

UniProtKB: Vacuolar fusion protein MON1

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Macromolecule #2: CCZ1/INTU/HSP4 first Longin domain-containing protein

MacromoleculeName: CCZ1/INTU/HSP4 first Longin domain-containing protein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Thermochaetoides thermophila (fungus)
Molecular weightTheoretical: 75.867539 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: SMTTPVSPSP SGIIPAQLGF LAIYNPALGT TDETLEDQIV YYATASTLSQ ARRRHRRPRR RDRQRAQSVV KDSRPNAAGA TGDSEAVAE DKDPVSKEER HERLRQIGLA QGMVEFAKSF SDGEPVDTID TEKARVILVE VEEGWWILAS IDLTRLPLPQ I KTPTSSSA ...String:
SMTTPVSPSP SGIIPAQLGF LAIYNPALGT TDETLEDQIV YYATASTLSQ ARRRHRRPRR RDRQRAQSVV KDSRPNAAGA TGDSEAVAE DKDPVSKEER HERLRQIGLA QGMVEFAKSF SDGEPVDTID TEKARVILVE VEEGWWILAS IDLTRLPLPQ I KTPTSSSA PPPAPNLNPL PPEPAYEYSS REVKPPSLLR ADLLRAYDLF LLHHGSSLSS LLASQGRAQL VASLTRFWDH FL ATWNVLL HGNPACDVFG GIKLAASGEL GIGVGEEERG SGEREVLEGL VERVEGLVDV VVGRYGGPPS EKGPEEEQWL GLG GEVGEE DGAVFLGVGA LDRKSLRGVV QWMEEVYVWG ENAFGKPRRD LSTGHFLLGL SECSEEELTS SQANPKAIFV ELKP SYQHP SRKIPPEDPQ PLGKVGPELP RDHTARLRPV IYVSQPFIYI LLFSEITPSP STWPTLAESL HAQLSPLQKP LLHST SYRP ERPVVETTSS SGTTTQHQIF DLVYDTETLT LQSTIPNIPD PFPYSATTPT GHSTGQQHHQ QSIWTRVEAL QTHAQI LAI LSSGRAIPTD PSSFTHLPWE EGERTCKTAR GWWIVWTRVV EHSPPDAVSL HHARDDDDND DDASCSVLGH LRSVSSS HA AGSTSSSSGS GFGLGAIPGL GGLGGWAADG ATRLAQGIGI DTRRYVEGLL TSLGR

UniProtKB: CCZ1/INTU/HSP4 first Longin domain-containing protein, CCZ1/INTU/HSP4 first Longin domain-containing protein

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.8 mg/mL
BufferpH: 7.3
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 1271173
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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