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- EMDB-54061: Cryo-EM structure of the CorM filament from cyanobacterium Anabae... -

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Basic information

Entry
Database: EMDB / ID: EMD-54061
TitleCryo-EM structure of the CorM filament from cyanobacterium Anabaena sp. PCC 7120
Map dataFull sharpened map of CorM filament
Sample
  • Complex: CorM filament from cyanobacterium Anabaena sp. PCC 7120
    • Protein or peptide: CorM
KeywordsCell shape / cytoskeleton / STRUCTURAL PROTEIN
Biological speciesNostoc sp. PCC 7120 = FACHB-418 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.14 Å
AuthorsSpringstein BL / Javoor MG / Megrian D / Hajdu R / Hanke DM / Schur FKM / Loose M
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
European Research Council (ERC)ActinID 101076260European Union
CitationJournal: Science / Year: 2026
Title: Repurposing of a DNA segregation machinery into a cytoskeletal system controlling cell shape
Authors: Springstein BL / Javoor MG / Megrian D / Hajdu R / Hanke DM / Schur FKM / Loose M
History
DepositionJun 18, 2025-
Header (metadata) releaseApr 8, 2026-
Map releaseApr 8, 2026-
UpdateApr 8, 2026-
Current statusApr 8, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_54061.png

Downloads & links

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Map

FileDownload / File: emd_54061.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationFull sharpened map of CorM filament
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.96 Å/pix.
x 512 pix.
= 489.011 Å		0.96 Å/pix.
x 512 pix.
= 489.011 Å		0.96 Å/pix.
x 512 pix.
= 489.011 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.9551 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.271
Minimum - Maximum-0.5281487 - 1.1178894
Average (Standard dev.)0.00051246653 (±0.040335372)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 489.0112 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_54061_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Full non-sharpened map of CorM filament

Fileemd_54061_additional_1.map
AnnotationFull non-sharpened map of CorM filament
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of CorM filament

Fileemd_54061_half_map_1.map
AnnotationHalf map of CorM filament
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map of CorM filament

Fileemd_54061_half_map_2.map
AnnotationHalf map of CorM filament
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : CorM filament from cyanobacterium Anabaena sp. PCC 7120

EntireName: CorM filament from cyanobacterium Anabaena sp. PCC 7120
Components
  • Complex: CorM filament from cyanobacterium Anabaena sp. PCC 7120
    • Protein or peptide: CorM

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Supramolecule #1: CorM filament from cyanobacterium Anabaena sp. PCC 7120

SupramoleculeName: CorM filament from cyanobacterium Anabaena sp. PCC 7120
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)

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Macromolecule #1: CorM

MacromoleculeName: CorM / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. PCC 7120 = FACHB-418 (bacteria)
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MTDQPSAATP MNAAAIPLNR VSASTPINAA PANNKPNNGS SKSILSVDLG RTSTKTCVGR EPNNVVFVPA NVKQMSIEQV RGGVFEARAT DPLMDLWLEY QGKGYAVGQL AADFGANLGV GQSKVEDALI KVLASAGYFK LKDEISVVLG LPFLSLEQFE REKAQLTSQV ...String:
MTDQPSAATP MNAAAIPLNR VSASTPINAA PANNKPNNGS SKSILSVDLG RTSTKTCVGR EPNNVVFVPA NVKQMSIEQV RGGVFEARAT DPLMDLWLEY QGKGYAVGQL AADFGANLGV GQSKVEDALI KVLASAGYFK LKDEISVVLG LPFLSLEQFE REKAQLTSQV TGPHVLNFRG ESVSLNITKV WVMPEGYGSL LWSEAQPKKG GASPDFTKIS TAIVDIGHQT IDLLMVDNFR FARGASKSED FGMNKFYELV AAEIDGADSQ SLALISAVNK PKGERFYRPK GASKPTNLDD SLPNLIEQFS REICSRVLAW LPERVTDVII TGGGGEFFWE DVQRLLKDAQ ISAHLAAPSR QANALGQYIY GEAQLSSNRA ARA

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
Details: 25 mM HEPES (pH 7.4), 150 mM KCl, 1 mM MgCl2, and 2 mM ATP
GridModel: Quantifoil R2/2 / Material: GOLD / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR / Details: Grids were glow discharged for 60s with 25mA.
VitrificationCryogen name: ETHANE / Chamber humidity: 80 % / Chamber temperature: 298.15 K / Instrument: LEICA EM GP

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 2206 / Average electron dose: 80.0 e/Å2 / Details: 4096x4096 px movies in EER format
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.9 µm / Nominal magnification: 150000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN

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Image processing

Particle selectionNumber selected: 5684956 / Details: Filament tracer was used to pick particles
CTF correctionSoftware - Name: cryoSPARC (ver. 4.4.0) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Details: Ab-initio reconstrcution was used for initial model
Final reconstructionNumber classes used: 2 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 7.14 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.4.0) / Number images used: 154726
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.4.0)
Final 3D classificationNumber classes: 3 / Software - Name: cryoSPARC (ver. 4.4.0)
Details: 2 classes out of 3 were selected for final reconstrcution
FSC plot (resolution estimation)

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