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- EMDB-53945: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state -

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Basic information

Entry
Database: EMDB / ID: EMD-53945
TitleCo-chaperone Bag1-bound human 26S proteasome in SBag2 state
Map dataB factor sharpened map
Sample
  • Complex: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state
KeywordsProtein degradation / protease / AAA+ ATPase / Hsp70 / cochaperone / Bag1 / Complex / ubiquitin / HYDROLASE
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsCheng TC / Sakata E / Muntaner J / Maestro-Lopez M / Cuellar J / Valpuesta JM
Funding support Germany, Spain, 5 items
OrganizationGrant numberCountry
German Research Foundation (DFG)154113120 Germany
German Research Foundation (DFG)EXC 2067/1-390729940 Germany
German Research Foundation (DFG)448415290 Germany
Spanish Ministry of Science, Innovation, and UniversitiesPID2022-137175NB-I00 Spain
Spanish Ministry of Science, Innovation, and UniversitiesAEI/10.13039/5011000110 Spain
CitationJournal: To Be Published
Title: Structures of the 26S proteasome in complex with cochaperone Bag1
Authors: Maestro-Lopez M / Cheng TC / Muntaner J / Menendez M / Alonso M / Schweitzer A / Cuellar J / Valpuesta JM / Sakta E
History
DepositionJun 5, 2025-
Header (metadata) releaseJan 14, 2026-
Map releaseJan 14, 2026-
UpdateJan 14, 2026-
Current statusJan 14, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53945.png

Downloads & links

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Map

FileDownload / File: emd_53945.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationB factor sharpened map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.41 Å/pix.
x 300 pix.
= 424. Å		1.41 Å/pix.
x 300 pix.
= 424. Å		1.41 Å/pix.
x 300 pix.
= 424. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.41333 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.016
Minimum - Maximum-0.036559395 - 0.08520918
Average (Standard dev.)0.0004073811 (±0.0027060253)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions300300300
Spacing300300300
CellA=B=C: 423.9999 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: enhanced map by LocScale-2.0

Fileemd_53945_additional_1.map
Annotationenhanced map by LocScale-2.0
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_53945_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_53945_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Co-chaperone Bag1-bound human 26S proteasome in SBag2 state

EntireName: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state
Components
  • Complex: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state

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Supramolecule #1: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state

SupramoleculeName: Co-chaperone Bag1-bound human 26S proteasome in SBag2 state
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
Component:
ConcentrationFormulaName
20.0 mMHepesHepes
150.0 mMKClPotassium chloride
1.0 mMDTTDithiothreitol
GridModel: Quantifoil R2/2 / Material: COPPER/RHODIUM / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 37.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: EMDB MAP
EMDB ID:

32283

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 59907
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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