EMDB-53843: Cryo-EM structure of the human pre-Bact-OTS complex (whole map)

Basic information

Entry

Database: EMDB / ID: EMD-53843

• Title: Cryo-EM structure of the human pre-Bact-OTS complex (whole map)

Sample

• Complex: OTS-stalled spliceosome complex
  • Protein or peptide: x 50 types
  • RNA: x 4 types

• Keywords: spliceosome / SPLICING

Function / homology

Function:

protein localization to P-body / DNA topoisomerase binding / RS domain binding / microfibril / Lsm2-8 complex / somatic diversification of immunoglobulins / U6 snRNA 3'-end binding / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / protein kinase B binding / U6 snRNP / PH domain binding / U11/U12 snRNP / regulation of retinoic acid receptor signaling pathway / interleukin-17-mediated signaling pathway / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / cis assembly of pre-catalytic spliceosome / histone pre-mRNA 3'end processing complex / regulation of vitamin D receptor signaling pathway / mRNA splice site recognition / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / B-WICH complex / alternative mRNA splicing, via spliceosome / protein localization to kinetochore / protein methylation / 7-methylguanosine cap hypermethylation / U12-type spliceosomal complex / nuclear retinoic acid receptor binding / embryonic brain development / U1 snRNP binding / U2-type catalytic step 1 spliceosome / methylosome / pICln-Sm protein complex / ATP-dependent activity, acting on RNA / response to alkaloid / RNA splicing, via transesterification reactions / positive regulation of mRNA splicing, via spliceosome / signal transduction involved in regulation of gene expression / mRNA 3'-end processing / sno(s)RNA-containing ribonucleoprotein complex / small nuclear ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / splicing factor binding / blastocyst formation / P granule / positive regulation of vitamin D receptor signaling pathway / snRNP binding / commitment complex / host-mediated activation of viral transcription / U2-type precatalytic spliceosome / mRNA cis splicing, via spliceosome / telomerase holoenzyme complex / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RNA polymerase binding / Notch binding / RUNX3 regulates NOTCH signaling / telomerase RNA binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / Transport of Mature mRNA derived from an Intron-Containing Transcript / U2-type spliceosomal complex / nuclear vitamin D receptor binding / NOTCH4 Intracellular Domain Regulates Transcription / transcription elongation factor activity / U1 snRNP / SAGA complex / U2 snRNP / RNA Polymerase II Transcription Termination / U4 snRNP / P-body assembly / NOTCH3 Intracellular Domain Regulates Transcription / : / U2-type prespliceosome / tRNA processing / Basigin interactions / mRNA stabilization / positive regulation of transcription by RNA polymerase III / positive regulation of neurogenesis / nuclear androgen receptor binding / ubiquitin-ubiquitin ligase activity / precatalytic spliceosome / Notch-HLH transcription pathway / WW domain binding / ubiquitin-like protein conjugating enzyme binding / Formation of paraxial mesoderm / mitotic spindle assembly checkpoint signaling / WD40-repeat domain binding / regulation of alternative mRNA splicing, via spliceosome / mRNA 5'-splice site recognition / positive regulation of transforming growth factor beta receptor signaling pathway / mRNA 3'-splice site recognition / SMAD binding / regulation of RNA splicing / mRNA catabolic process

Domain/homology:

: / SR140, RNA recognition motif / WW domain binding protein 11 / WW domain binding protein 11 / Protein RED, C-terminal / RED-like, N-terminal / Beta-catenin-like protein 1, N-terminal / Beta-catenin-like protein 1 / Protein Red-like / RED-like protein C-terminal region / RED-like protein N-terminal region / Catenin-beta-like, Arm-motif containing nuclear / Eukaryotic domain of unknown function (DUF1716) / : / SRSF1, RNA recognition motif 1 / : / WD40 repeat-containing protein SMU1 / Peptidyl-prolyl cis-trans isomerase like 2, U-box domain / Ubiquitin-like modifier Hub1/Ubl5 / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal / Microfibrillar-associated protein 1 / Microfibril-associated/Pre-mRNA processing / DHX15, DEXH-box helicase domain / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / Transcription elongation regulator 1-like / : / PRP38 family / TCERG1 WW domain / : / LisH-like dimerisation domain / cwf21 / FF domain / C-terminal to LisH motif. / CID domain / RPR / FF domain / CID domain / FF domain superfamily / CID domain profile. / FF domain profile. / Contains two conserved F residues / Sm-like protein Lsm8 / CTLH, C-terminal LisH motif / SF3B6, RNA recognition motif / C-terminal to LisH (CTLH) motif profile. / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Sm-like protein Lsm7 / U6 snRNA-associated Sm-like protein Lsm1/8 / Sm-like protein LSm5 / Sm-like protein Lsm3 / U6 snRNA-associated Sm-like protein Lsm3 / SF3B4, RNA recognition motif 2 / Splicing factor SF3a60 binding domain / Splicing factor SF3a60 binding domain / Cactus-binding C-terminus of cactin protein / Splicing factor 3A subunit 1, ubiquitin domain / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / : / Replication stress response SDE2 C-terminal / mRNA splicing factor Cwf21 domain / cwf21 domain / SF3A2 domain / : / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / Splicing factor SF3a60 /Prp9 subunit, C-terminal / SF3A3 domain / SF3a60/Prp9 C-terminal / Pre-mRNA-splicing factor SF3A3, of SF3a complex, Prp9 / Pre-mRNA-processing factor 17 / SF3B4, RNA recognition motif 1 / : / Splicing factor 3A subunit 1, conserved domain / Splicing factor 3A subunit 1 / Pre-mRNA splicing factor PRP21 like protein / G10 protein signature 2. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase, cyclophilin A-like / : / BUD31/G10-related, conserved site / : / G10 protein signature 1. / Domain of unknown function DUF382 / Domain of unknown function (DUF382) / SWAP/Surp / SWAP/Surp superfamily / Surp module / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator / Splicing factor 3B, subunit 5 / SKI-interacting protein SKIP, SNW domain / Pre-mRNA-splicing factor Cwf15/Cwc15 / SKI-interacting protein, SKIP / SKIP/SNW domain / Cwf15/Cwc15 cell cycle control protein / Splicing factor 3B subunit 1 / Splicing factor 3B subunit 1

Component:

Transcription elongation regulator 1 / U2 snRNP-associated SURP motif-containing protein / ATP-dependent RNA helicase DHX15 / Pleiotropic regulator 1 / Pre-mRNA-processing factor 17 / Splicing factor 3B subunit 1 / U5 small nuclear ribonucleoprotein 200 kDa helicase / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Protein BUD31 homolog / Microfibrillar-associated protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / U6 snRNA-associated Sm-like protein LSm3 / U6 snRNA-associated Sm-like protein LSm6 / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Serine/arginine-rich splicing factor 1 / Splicing factor 3A subunit 3 / Protein Red / RING-type E3 ubiquitin-protein ligase PPIL2 / Splicing factor 3B subunit 2 / SNW domain-containing protein 1 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3B subunit 3 / Splicing factor 3B subunit 4 / Splicing factor 3A subunit 2 / Splicing factor 3A subunit 1 / WD40 repeat-containing protein SMU1 / PHD finger-like domain-containing protein 5A / Pre-mRNA-splicing factor 38A / Beta-catenin-like protein 1 / U5 small nuclear ribonucleoprotein 40 kDa protein / Zinc finger matrin-type protein 2 / Cell division cycle 5-like protein / Splicing factor 3B subunit 5 / Ubiquitin-like protein 5 / Spliceosome-associated protein CWC15 homolog / U6 snRNA-associated Sm-like protein LSm7 / WW domain-binding protein 11 / U6 snRNA-associated Sm-like protein LSm2 / Splicing factor 3B subunit 6 / U6 snRNA-associated Sm-like protein LSm5 / U6 snRNA-associated Sm-like protein LSm4

• Biological species: Homo sapiens (human)
• Method: single particle reconstruction / cryo EM / Resolution: 8.5 Å
• Authors: Zhang Z / Kumar V / Zhong J / Dybkov O / Kastner B / Urlaub H / Luhrmann R

Funding support

Germany, 1 items

Organization	Grant number	Country
German Research Foundation (DFG)		Germany

• Citation: Journal: To Be Published; Title: cryo-EM structure of the human pre-Bact-OTS complex; Authors: Zhang Z / Kumar V / Dybkov O / Zhong J / Kastner B / Henning U / Luehrmann R

History

Deposition	May 17, 2025	-
Header (metadata) release	May 27, 2026	-
Map release	May 27, 2026	-
Update	May 27, 2026	-
Current status	May 27, 2026	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_53843.map.gz / Format: CCP4 / Size: 52.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 2.7 Å

Density

Contour Level	By AUTHOR: 0.0021
Minimum - Maximum	-0.0037887797 - 0.012734752
Average (Standard dev.)	0.000038587612 (±0.00047008117)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 240 240 240 Spacing 240 240 240
Cell	A=B=C: 648.0 Å α=β=γ: 90.0 °

Supplemental data

Half map: #2

• File: emd_53843_half_map_1.map

Half map: #1

• File: emd_53843_half_map_2.map

Sample components

Entire : OTS-stalled spliceosome complex

• Entire: Name: OTS-stalled spliceosome complex

Components

• Complex: OTS-stalled spliceosome complex
  • Protein or peptide: Pre-mRNA-processing-splicing factor 8
  • Protein or peptide: U5 small nuclear ribonucleoprotein 200 kDa helicase
  • RNA: U2 snRNA
  • RNA: U6 snRNA
  • Protein or peptide: Splicing factor 3A subunit 1
  • RNA: U5 snRNA
  • Protein or peptide: Cell division cycle 5-like protein
  • Protein or peptide: 116 kDa U5 small nuclear ribonucleoprotein component
  • Protein or peptide: U5 small nuclear ribonucleoprotein 40 kDa protein
  • Protein or peptide: Pleiotropic regulator 1
  • Protein or peptide: Pre-mRNA-splicing factor 38A
  • Protein or peptide: Protein BUD31 homolog
  • Protein or peptide: Spliceosome-associated protein CWC15 homolog
  • Protein or peptide: WW domain-binding protein 11
  • Protein or peptide: Ubiquitin-like protein 5
  • Protein or peptide: Zinc finger matrin-type protein 2
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D2
  • Protein or peptide: U2 small nuclear ribonucleoprotein B''
  • Protein or peptide: Small nuclear ribonucleoprotein F
  • Protein or peptide: Small nuclear ribonucleoprotein-associated proteins B and B'
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D3
  • Protein or peptide: Small nuclear ribonucleoprotein G
  • Protein or peptide: Small nuclear ribonucleoprotein E
  • Protein or peptide: Small nuclear ribonucleoprotein Sm D1
  • Protein or peptide: U2 small nuclear ribonucleoprotein A'
  • Protein or peptide: Splicing factor 3B subunit 4
  • Protein or peptide: Splicing factor 3A subunit 3
  • Protein or peptide: Splicing factor 3A subunit 2
  • Protein or peptide: Splicing factor 3B subunit 2
  • Protein or peptide: Splicing factor 3B subunit 5
  • Protein or peptide: Splicing factor 3B subunit 3
  • Protein or peptide: Splicing factor 3B subunit 1
  • Protein or peptide: Splicing factor 3B subunit 6
  • Protein or peptide: Beta-catenin-like protein 1
  • Protein or peptide: U2 snRNP-associated SURP motif-containing protein
  • Protein or peptide: ATP-dependent RNA helicase DHX15
  • RNA: pre-mRNA
  • Protein or peptide: Protein Red
  • Protein or peptide: WD40 repeat-containing protein SMU1
  • Protein or peptide: Pre-mRNA-splicing factor RBM22
  • Protein or peptide: RING-type E3 ubiquitin-protein ligase PPIL2
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm2
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm3
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm4
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm5
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm6
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm7
  • Protein or peptide: U6 snRNA-associated Sm-like protein LSm8
  • Protein or peptide: SNW domain-containing protein 1
  • Protein or peptide: Serine/arginine-rich splicing factor 1
  • Protein or peptide: Transcription elongation regulator 1
  • Protein or peptide: Microfibrillar-associated protein 1
  • Protein or peptide: Pre-mRNA-processing factor 17
  • Protein or peptide: PHD finger-like domain-containing protein 5A

Supramolecule #1: OTS-stalled spliceosome complex

• Supramolecule: Name: OTS-stalled spliceosome complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#54
• Source (natural): Organism: Homo sapiens (human)

Macromolecule #1: Pre-mRNA-processing-splicing factor 8

• Macromolecule: Name: Pre-mRNA-processing-splicing factor 8 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 325.927906 KDa

Sequence

String:

FVDAQKEDMP PEHVRKIIRD HGDMTNRKFR HDKRVYLGAL KYMPHAVLKL LENMPMPWEQ IRDVPVLYHI TGAISFVNEI PWVIEPVYI SQWGSMWIMM RREKRDRRHF KRMRFPPFDD EEPPLDYADN ILDVEPLEAI QLELDPEEDA PVLDWFYDHQ P LRDSRKYV NGSTYQRWQF TLPMMSTLYR LANQLLTDLV DDNYFYLFDL KAFFTSKALN MAIPGGPKFE PLVRDINLQD ED WNEFNDI NKIIIRQPIR TEYKIAFPYL YNNLPHHVHL TWYHTPNVVF IKTEDPDLPA FYFDPLINPI SHRHSVKSQE PLP DDDEEF ELPEFVEPFL KDTPLYTDNT ANGIALLWAP RPFNLRSGRT RRALDIPLVK NWYREHCPAG QPVKVRVSYQ KLLK YYVLN ALKHRPPKAQ KKRYLFRSFK ATKFFQSTKM AGVFPYRGPG NPVPGPLAPL PDYMSEEKLQ EKARKWQQLQ AKRYA EKRK FGFVDAQKED MPPEHVRKII RDHGDMTNRK FRHDKRVYLG ALKYMPHAVL KLLENMPMPW EQIRDVPVLY HITGAI SFV NEIPWVIEPV YISQWGSMWI MMRREKRDRR HFKRMRFPPF DDEEPPLDYA DNILDVEPLE AIQLELDPEE DAPVLDW FY DHQPLRDSRK YVNGSTYQRW QFTLPMMSTL YRLANQLLTD LVDDNYFYLF DLKAFFTSKA LNMAIPGGPK FEPLVRDI N LQDEDWNEFN DINKIIIRQP IRTEYKIAFP YLYNNLPHHV HLTWYHTPNV VFIKTEDPDL PAFYFDPLIN PISHRHSVK SQEPLPDDDE EFELPEFVEP FLKDTPLYTD NTANGIALLW APRPFNLRSG RTRRALDIPL VKNWYREHCP AGQPVKVRVS YQKLLKYYV LNALKHRPPK AQKKRYLFRS FKATKFFQST KLDWVEVGLQ VCRQGYNMLN LLIHRKNLNY LHLDYNFNLK P VKTLTTKE RKKSRFGNAF HLCREVLRLT KLVVDSHVQY RLGNVDAFQL ADGLQYIFAH VGQLTGMYRY KYKLMRQIRM CK DLKHLIY YRFNTGPVGK GPGCGFWAAG WRVWLFFMRG ITPLLERWLG NLLARQFEGR HSKGVAKTVT KQRVESHFDL ELR AAVMHD ILDMMPEGIK QNKARTILQH LSEAWRCWKA NIPWKVPGLP TPIENMILRY VKAKADWWTN TAHYNRERIR RGAT VDKTV CKKNLGRLTR LYLKAEQERQ HNYLKDGPYI TAEEAVAVYT TTVHWLESRR FSPIPFPPLS YKHDTKLLIL ALERL KEAY SVKSRLNQSQ REELGLIEQA YDNPHEALSR IKRHLLTQRA FKEVGIEFMD LYSHLVPVYD VEPLEKITDA YLDQYL WYE ADKRRLFPPW IKPADTEPPP LLVYKWCQGI NNLQDVWETS EGECNVMLES RFEKMYEKID LTLLNRLLRL IVDHNIA DY MTAKNNVVIN YKDMNHTNSY GIIRGLQFAS FIVQYYGLVM DLLVLGLHRA SEMAGPPQMP NDFLSFQDIA TEAAHPIR L FCRYIDRIHI FFRFTADEAR DLIQRYLTEH PDPNNENIVG YNNKKCWPRD ARMRLMKHDV NLGRAVFWDI KNRLPRSVT TVQWENSFVS VYSKDNPNLL FNMCGFECRI LPKCRTSYEE FTHKDGVWNL QNEVTKERTA QCFLRVDDES MQRFHNRVRQ ILMASGSTT FTKIVNKWNT ALIGLMTYFR EAVVNTQELL DLLVKCENKI QTRIKIGLNS KMPSRFPPVV FYTPKELGGL G MLSMGHVL IPQSDLRWSK QTDVGITHFR SGMSHEEDQL IPNLYRYIQP WESEFIDSQR VWAEYALKRQ EAIAQNRRLT LE DLEDSWD RGIPRINTLF QKDRHTLAYD KGWRVRTDFK QYQVLKQNPF WWTHQRHDGK LWNLNNYRTD MIQALGGVEG ILE HTLFKG TYFPTWEGLF WEKASGFEES MKWKKLTNAQ RSGLNQIPNR RFTLWWSPTI NRANVYVGFQ VQLDLTGIFM HGKI PTLKI SLIQIFRAHL WQKIHESIVM DLCQVFDQEL DALEIETVQK ETIHPRKSYK MNSSCADILL FASYKWNVSR PSLLA DSKD VMDSTTTQKY WIDIQLRWGD YDSHDIERYA RAKFLDYTTD NMSIYPSPTG VLIAIDLAYN LHSAYGNWFP GSKPLI QQA MAKIMKANPA LYVLRERIRK GLQLYSSEPT EPYLSSQNYG ELFSNQIIWF VDDTNVYRVT IHKTFEGNLT TKPINGA IF IFNPRTGQLF LKIIHTSVWA GQKRLGQLAK WKTAEEVAAL IRSLPVEEQP KQIIVTRKGM LDPLEVHLLD FPNIVIKG S ELQLPFQACL KVEKFGDLIL KATEPQMVLF NLYDDWLKTI SSYTAFSRLI LILRALHVNN DRAKVILKPD KTTITEPHH IWPTLTDEEW IKVEVQLKDL ILADYGKKNN VNVASLTQSE IRDIILGMEI SAPSQQRQQI AEIEKQTKEQ SQLTATQTRT VNKHGDEII TSTTSNYETQ TFSSKTEWRV RAISAANLHL RTNHIYVSSD DIKETGYTYI LPKNVLKKFI CISDLRAQIA G YLYGVSPP DNPQVKEIRC IVMVPQWGTH QTVHLPGQLP QHEYLKEMEP LGWIHTQPNE SPQLSPQDVT THAKIMADNP SW DGEKTII ITCSFTPGSC TLTAYKLTPS GYEWGRQNTD KGNNPKGYLP SHYERVQMLL SDRFLGFFMV PAQSSWNYNF MGV RHDPNM KYELQLANPK EFYHEVHRPS HFLNFALLQE GEVYSADRED LYA

Macromolecule #2: U5 small nuclear ribonucleoprotein 200 kDa helicase

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 200 kDa helicase / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 244.823422 KDa

Sequence

String:

MADVTARSLQ YEYKANSNLV LQADRSLIDR TRRDEPTGEV LSLVGKLEGT RMGDKAQRTK PQMQEERRAK RRKRDEDRHD INKMKGYTL LSEGIDEMVG IIYKPKTKET RETYEVLLSF IQAALGDQPR DILCGAADEV LAVLKNEKLR DKERRKEIDL L LGQTDDTR YHVLVNLGKK ITDYGGDKEI QNMDDNIDET YGVNVQFESD EEEGDEDVYG EVREEASDDD MEGDEAVVRC TL SANLVAS GELMSSKKKD LHPRDIDAFW LQRQLSRFYD DAIVSQKKAD EVLEILKTAS DDRECENQLV LLLGFNTFDF IKV LRQHRM MILYCTLLAS AQSEAEKERI MGKMEADPEL SKFLYQLHET EKEDLIREER SRRERVRQSR MDTDLETMDL DQGG EALAP RQVLDLEDLV FTQGSHFMAN KRCQLPDGSF RRQRKGYEEV HVPALKPKPF GSEEQLLPVE KLPKYAQAGF EGFKT LNRI QSKLYRAALE TDENLLLCAP TGAGKTNVAL MCMLREIGKH INMDGTINVD DFKIIYIAPM RSLVQEMVGS FGKRLA TYG ITVAELTGDH QLCKEEISAT QIIVCTPEKW DIITRKGGER TYTQLVRLII LDEIHLLHDD RGPVLEALVA RAIRNIE MT QEDVRLIGLS ATLPNYEDVA TFLRVDPAKG LFYFDNSFRP VPLEQTYVGI TEKKAIKRFQ IMNEIVYEKI MEHAGKNQ V LVFVHSRKET GKTARAIRDM CLEKDTLGLF LREGSASTEV LRTEAEQCKN LELKDLLPYG FAIHHAGMTR VDRTLVEDL FADKHIQVLV STATLAWGVN LPAHTVIIKG TQVYSPEKGR WTELGALDIL QMLGRAGRPQ YDTKGEGILI TSHGELQYYL SLLNQQLPI ESQMVSKLPD MLNAEIVLGN VQNAKDAVNW LGYAYLYIRM LRSPTLYGIS HDDLKGDPLL DQRRLDLVHT A ALMLDKNN LVKYDKKTGN FQVTELGRIA SHYYITNDTV QTYNQLLKPT LSEIELFRVF SLSSEFKNIT VREEEKLELQ KL LERVPIP VKESIEEPSA KINVLLQAFI SQLKLEGFAL MADMVYVTQS AGRLMRAIFE IVLNRGWAQL TDKTLNLCKM IDK RMWQSM CPLRQFRKLP EEVVKKIEKK NFPFERLYDL NHNEIGELIR MPKMGKTIHK YVHLFPKLEL SVHLQPITRS TLKV ELTIT PDFQWDEKVH GSSEAFWILV EDVDSEVILH HEYFLLKAKY AQDEHLITFF VPVFEPLPPQ YFIRVVSDRW LSCET QLPV SFRHLILPEK YPPPTELLDL QPLPVSALRN SAFESLYQDK FPFFNPIQTQ VFNTVYNSDD NVFVGAPTGS GKTICA EFA ILRMLLQSSE GRCVYITPME ALAEQVYMDW YEKFQDRLNK KVVLLTGETS TDLKLLGKGN IIISTPEKWD ILSRRWK QR KNVQNINLFV VDEVHLIGGE NGPVLEVICS RMRYISSQIE RPIRIVALSS SLSNAKDVAH WLGCSATSTF NFHPNVRP V PLELHIQGFN ISHTQTRLLS MAKPVYHAIT KHSPKKPVIV FVPSRKQTRL TAIDILTTCA ADIQRQRFLH CTEKDLIPY LEKLSDSTLK ETLLNGVGYL HEGLSPMERR LVEQLFSSGA IQVVVASRSL CWGMNVAAHL VIIMDTQYYN GKIHAYVDYP IYDVLQMVG HANRPLQDDE GRCVIMCQGS KKDFFKKFLY EPLPVESHLD HCMHDHFNAE IVTKTIENKQ DAVDYLTWTF L YRRMTQNP NYYNLQGISH RHLSDHLSEL VEQTLSDLEQ SKCISIEDEM DVAPLNLGMI AAYYYINYTT IELFSMSLNA KT KVRGLIE IISNAAEYEN IPIRHHEDNL LRQLAQKVPH KLNNPKFNDP HVKTNLLLQA HLSRMQLSAE LQSDTEEILS KAI RLIQAC VDVLSSNGWL SPALAAMELA QMVTQAMWSK DSYLKQLPHF TSEHIKRCTD KGVESVFDIM EMEDEERNAL LQLT DSQIA DVARFCNRYP NIELSYEVVD KDSIRSGGPV VVLVQLEREE EVTGPVIAPL FPQKREEGWW VVIGDAKSNS LISIK RLTL QQKAKVKLDF VAPATGAHNY TLYFMSDAYM GCDQEYKFSV DVKEAETDSD SD

UniProtKB: U5 small nuclear ribonucleoprotein 200 kDa helicase

Macromolecule #5: Splicing factor 3A subunit 1

• Macromolecule: Name: Splicing factor 3A subunit 1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 88.991094 KDa

Sequence

String:

MPAGPVQAVP PPPPVPTEPK QPTEEEASSK EDSAPSKPVV GIIYPPPEVR NIVDKTASFV ARNGPEFEAR IRQNEINNPK FNFLNPNDP YHAYYRHKVS EFKEGKAQEP SAAIPKVMQQ QQQTTQQQLP QKVQAQVIQE TIVPKEPPPE FEFIADPPSI S AFDLDVVK LTAQFVARNG RQFLTQLMQK EQRNYQFDFL RPQHSLFNYF TKLVEQYTKI LIPPKGLFSK LKKEAENPRE VL DQVCYRV EWAKFQERER KKEEEEKEKE RVAYAQIDWH DFVVVETVDF QPNEQGNFPP PTTPEELGAR ILIQERYEKF GES EEVEME VESDEEDDKQ EKAEEPPSQL DQDTQVQDMD EGSDDEEEGQ KVPPPPETPM PPPLPPTPDQ VIVRKDYDPK ASKP LPPAP APDEYLVSPI TGEKIPASKM QEHMRIGLLD PRWLEQRDRS IREKQSDDEV YAPGLDIESS LKQLAERRTD IFGVE ETAI GKKIGEEEIQ KPEEKVTWDG HSGSMARTQQ AAQANITLQE QIEAIHKAKG LVPEDDTKEK IGPSKPNEIP QQPPPP SSA TNIPSSAPPI TSVPRPPTMP PPVRTTVVSA VPVMPRPPMA SVVRLPPGSV IAPMPPIIHA PRINVVPMPP SAPPIMA PR PPPMIVPTAF VPAPPVAPVP APAPMPPVHP PPPMEDEPTS KKLKTEDSLM PEEEFLRRNK GPVSIKVQVP NMQDKTEW K LNGQVLVFTL PLTDQVSVIK VKIHEATGMP AGKQKLQYEG IFIKDSNSLA YYNMANGAVI HLALKERGGR KK

UniProtKB: Splicing factor 3A subunit 1

Macromolecule #7: Cell division cycle 5-like protein

• Macromolecule: Name: Cell division cycle 5-like protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 92.406883 KDa

Sequence

String:

MPRIMIKGGV WRNTEDEILK AAVMKYGKNQ WSRIASLLHR KSAKQCKARW YEWLDPSIKK TEWSREEEEK LLHLAKLMPT QWRTIAPII GRTAAQCLEH YEFLLDKAAQ RDNEEETTDD PRKLKPGEID PNPETKPARP DPIDMDEDEL EMLSEARARL A NTQGKKAK RKAREKQLEE ARRLAALQKR RELRAAGIEI QKKRKRKRGV DYNAEIPFEK KPALGFYDTS EENYQALDAD FR KLRQQDL DGELRSEKEG RDRKKDKQHL KRKKESDLPS AILQTSGVSE FTKKRSKLVL PAPQISDAEL QEVVKVGQAS EIA RQTAEE SGITNSASST LLSEYNVTNN SVALRTPRTP ASQDRILQEA QNLMALTNVD TPLKGGLNTP LHESDFSGVT PQRQ VVQTP NTVLSTPFRT PSNGAEGLTP RSGTTPKPVI NSTPGRTPLR DKLNINPEDG MADYSDPSYV KQMERESREH LRLGL LGLP APKNDFEIVL PENAEKELEE REIDDTYIED AADVDARKQA IRDAERVKEM KRMHKAVQKD LPRPSEVNET ILRPLN VEP PLTDLQKSEE LIKKEMITML HYDLLHHPYE PSGNKKGKTV GFGTNNSEHI TYLEHNPYEK FSKEELKKAQ DVLVQEM EV VKQGMSHGEL SSEAYNQVWE ECYSQVLYLP GQSRYTRANL ASKKDRIESL EKRLEINRGH MTTEAKRAAK MEKKMKIL L GGYQSRAMGL MKQLNDLWDQ IEQAHLELRT FEELKKHEDS AIPRRLECLK EDVQRQQERE KELQHRYADL LLEKETLKS KF

UniProtKB: Cell division cycle 5-like protein

Macromolecule #8: 116 kDa U5 small nuclear ribonucleoprotein component

• Macromolecule: Name: 116 kDa U5 small nuclear ribonucleoprotein component / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 109.560625 KDa

Sequence

String:

MDTDLYDEFG NYIGPELDSD EDDDELGRET KDLDEMDDDD DDDDVGDHDD DHPGMEVVLH EDKKYYPTAE EVYGPEVETI VQEEDTQPL TEPIIKPVKT KKFTLMEQTL PVTVYEMDFL ADLMDNSELI RNVTLCGHLH HGKTCFVDCL IEQTHPEIRK R YDQDLCYT DILFTEQERG VGIKSTPVTV VLPDTKGKSY LFNIMDTPGH VNFSDEVTAG LRISDGVVLF IDAAEGVMLN TE RLIKHAV QERLAVTVCI NKIDRLILEL KLPPTDAYYK LRHIVDEVNG LISMYSTDEN LILSPLLGNV CFSSSQYSIC FTL GSFAKI YADTFGDINY QEFAKRLWGD IYFNPKTRKF TKKAPTSSSQ RSFVEFILEP LYKILAQVVG DVDTSLPRTL DELG IHLTK EELKLNIRPL LRLVCKKFFG EFTGFVDMCV QHIPSPKVGA KPKIEHTYTG GVDSDLGEAM SDCDPDGPLM CHTTK MYST DDGVQFHAFG RVLSGTIHAG QPVKVLGENY TLEDEEDSQI CTVGRLWISV ARYHIEVNRV PAGNWVLIEG VDQPIV KTA TITEPRGNEE AQIFRPLKFN TTSVIKIAVE PVNPSELPKM LDGLRKVNKS YPSLTTKVEE SGEHVILGTG ELYLDCV MH DLRKMYSEID IKVADPVVTF CETVVETSSL KCFAETPNKK NKITMIAEPL EKGLAEDIEN EVVQITWNRK KLGEFFQT K YDWDLLAARS IWAFGPDATG PNILVDDTLP SEVDKALLGS VKDSIVQGFQ WGTREGPLCD ELIRNVKFKI LDAVVAQEP LHRGGGQIIP TARRVVYSAF LMATPRLMEP YYFVEVQAPA DCVSAVYTVL ARRRGHVTQD APIPGSPLYT IKAFIPAIDS FGFETDLRT HTQGQAFSLS VFHHWQIVPG DPLDKSIVIR PLEPQPAPHL AREFMIKTRR RKGLSEDVSI SKFFDDPMLL E LAKQDVVL NYPM

UniProtKB: 116 kDa U5 small nuclear ribonucleoprotein component

Macromolecule #9: U5 small nuclear ribonucleoprotein 40 kDa protein

• Macromolecule: Name: U5 small nuclear ribonucleoprotein 40 kDa protein / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 39.359492 KDa

Sequence

String:

MIEQQKRKGP ELPLVPVKRQ RHELLLGAGS GPGAGQQQAT PGALLQAGPP RCSSLQAPIM LLSGHEGEVY CCKFHPNGST LASAGFDRL ILLWNVYGDC DNYATLKGHS GAVMELHYNT DGSMLFSAST DKTVAVWDSE TGERVKRLKG HTSFVNSCYP A RRGPQLVC TGSDDGTVKL WDIRKKAAIQ TFQNTYQVLA VTFNDTSDQI ISGGIDNDIK VWDLRQNKLT YTMRGHADSV TG LSLSSEG SYLLSNAMDN TVRVWDVRPF APKERCVKIF QGNVHNFEKN LLRCSWSPDG SKIAAGSADR FVYVWDTTSR RIL YKLPGH AGSINEVAFH PDEPIIISAS SDKRLYMGEI Q

UniProtKB: U5 small nuclear ribonucleoprotein 40 kDa protein

Macromolecule #10: Pleiotropic regulator 1

• Macromolecule: Name: Pleiotropic regulator 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.280758 KDa

Sequence

String:

MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT SKENLKEKGP QNATDSYVHK QYPANQGQE VEYFVAGTHP YPPGPGVALT ADTKIQRMPS ESAAQSLAVA LPLQTKADAN RTAPSGSEYR HPGASDRPQP T AMNSIVME TGNTKNSALM AKKAPTMPKP QWHPPWKLYR VISGHLGWVR CIAVEPGNQW FVTGSADRTI KIWDLASGKL KL SLTGHIS TVRGVIVSTR SPYLFSCGED KQVKCWDLEY NKVIRHYHGH LSAVYGLDLH PTIDVLVTCS RDSTARIWDV RTK ASVHTL SGHTNAVATV RCQAAEPQII TGSHDTTIRL WDLVAGKTRV TLTNHKKSVR AVVLHPRHYT FASGSPDNIK QWKF PDGSF IQNLSGHNAI INTLTVNSDG VLVSGADNGT MHLWDWRTGY NFQRVHAAVQ PGSLDSESGI FACAFDQSES RLLTA EADK TIKVYREDDT ATEETHPVSW KPEIIKRKRF

UniProtKB: Pleiotropic regulator 1

Macromolecule #11: Pre-mRNA-splicing factor 38A

• Macromolecule: Name: Pre-mRNA-splicing factor 38A / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.563863 KDa

Sequence

String:

MANRTVKDAH SIHGTNPQYL VEKIIRTRIY ESKYWKEECF GLTAELVVDK AMELRFVGGV YGGNIKPTPF LCLTLKMLQI QPEKDIIVE FIKNEDFKYV RMLGALYMRL TGTAIDCYKY LEPLYNDYRK IKSQNRNGEF ELMHVDEFID ELLHSERVCD I ILPRLQKR YVLEEAEQLE PRVSALEEDM DDVESSEEEE EEDEKLERVP SPDHRRRSYR DLDKPRRSPT LRYRRSRSRS PR RRSRSPK RRSPSPRRER HRSKSPRRHR SRSRDRRHRS RSKSPGHHRS HRHRSHSKSP ERSKKSHKKS RRGNE

UniProtKB: Pre-mRNA-splicing factor 38A

Macromolecule #12: Protein BUD31 homolog

• Macromolecule: Name: Protein BUD31 homolog / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 17.03285 KDa

Sequence

String:

MPKVKRSRKA PPDGWELIEP TLDELDQKMR EAETEPHEGK RKVESLWPIF RIHHQKTRYI FDLFYKRKAI SRELYEYCIK EGYADKNLI AKWKKQGYEN LCCLRCIQTR DTNFGTNCIC RVPKSKLEVG RIIECTHCGC RGCSG

UniProtKB: Protein BUD31 homolog

Macromolecule #13: Spliceosome-associated protein CWC15 homolog

• Macromolecule: Name: Spliceosome-associated protein CWC15 homolog / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 26.674447 KDa

Sequence

String:

MTTAARPTFE PARGGRGKGE GDLSQLSKQY SSRDLPSHTK IKYRQTTQDA PEEVRNRDFR RELEERERAA AREKNRDRPT REHTTSSSV SKKPRLDQIP AANLDADDPL TDEEDEDFEE ESDDDDTAAL LAELEKIKKE RAEEQARKEQ EQKAEEERIR M ENILSGNP LLNLTGPSQP QANFKVKRRW DDDVVFKNCA KGVDDQKKDK RFVNDTLRSE FHKKFMEKYI K

UniProtKB: Spliceosome-associated protein CWC15 homolog

Macromolecule #14: WW domain-binding protein 11

• Macromolecule: Name: WW domain-binding protein 11 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 70.098641 KDa

Sequence

String:

MGRRSTSSTK SGKFMNPTDQ ARKEARKREL KKNKKQRMMV RAAVLKMKDP KQIIRDMEKL DEMEFNPVQQ PQLNEKVLKD KRKKLRETF ERILRLYEKE NPDIYKELRK LEVEYEQKRA QLSQYFDAVK NAQHVEVESI PLPDMPHAPS NILIQDIPLP G AQPPSILK KTSAYGPPTR AVSILPLLGH GVPRLPPGRK PPGPPPGPPP PQVVQMYGRK VGFALDLPPR RRDEDMLYSP EL AQRGHDD DVSSTSEDDG YPEDMDQDKH DDSTDDSDTD KSDGESDGDE FVHRDNGERD NNEEKKSGLS VRFADMPGKS RKK KKNMKE LTPLQAMMLR MAGQEIPEEG REVEEFSEDD DEDDSDDSEA EKQSQKQHKE ESHSDGTSTA SSQQQAPPQS VPPS QIQAP PMPGPPPLGP PPAPPLRPPG PPTGLPPGPP PGAPPFLRPP GMPGLRGPLP RLLPPGPPPG RPPGPPPGPP PGLPP GPPP RGPPPRLPPP APPGIPPPRP GMMRPPLVPP LGPAPPGLFP PAPLPNPGVL SAPPNLIQRP KADDTSAATI EKKATA TIS AKPQITNPKA EITRFVPTAL RVRRENKGAT AAPQRKSEDD SAVPLAKAAP KSGPSVPVSV QTKDDVYEAF MKEMEGL L

UniProtKB: WW domain-binding protein 11

Macromolecule #15: Ubiquitin-like protein 5

• Macromolecule: Name: Ubiquitin-like protein 5 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.560945 KDa

Sequence

String:

MIEVVCNDRL GKKVRVKCNT DDTIGDLKKL IAAQTGTRWN KIVLKKWYTI FKDHVSLGDY EIHDGMNLEL YYQ

UniProtKB: Ubiquitin-like protein 5

Macromolecule #16: Zinc finger matrin-type protein 2

• Macromolecule: Name: Zinc finger matrin-type protein 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 23.664047 KDa

Sequence

String:

MASGSGTKNL DFRRKWDKDE YEKLAEKRLT EEREKKDGKP VQPVKRELLR HRDYKVDLES KLGKTIVITK TTPQSEMGGY YCNVCDCVV KDSINFLDHI NGKKHQRNLG MSMRVERSTL DQVKKRFEVN KKKMEEKQKD YDFEERMKEL REEEEKAKAY K KEKQKEKK RRAEEDLTFE EDDEMAAVMG FSGFGSTKKS Y

UniProtKB: Zinc finger matrin-type protein 2

Macromolecule #17: Small nuclear ribonucleoprotein Sm D2

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D2 / type: protein_or_peptide / ID: 17 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.551928 KDa

Sequence

String:

MSLLNKPKSE MTPEELQKRE EEEFNTGPLS VLTQSVKNNT QVLINCRNNK KLLGRVKAFD RHCNMVLENV KEMWTEVPKS GKGKKKSKP VNKDRYISKM FLRGDSVIVV LRNPLIAGK

UniProtKB: Small nuclear ribonucleoprotein Sm D2

Macromolecule #18: U2 small nuclear ribonucleoprotein B''

• Macromolecule: Name: U2 small nuclear ribonucleoprotein B'' / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 25.524367 KDa

Sequence

String:

MDIRPNHTIY INNMNDKIKK EELKRSLYAL FSQFGHVVDI VALKTMKMRG QAFVIFKELG SSTNALRQLQ GFPFYGKPMR IQYAKTDSD IISKMRGTFA DKEKKKEKKK AKTVEQTATT TNKKPGQGTP NSANTQGNST PNPQVPDYPP NYILFLNNLP E ETNEMMLS MLFNQFPGFK EVRLVPGRHD IAFVEFENDG QAGAARDALQ GFKITPSHAM KITYAKK

UniProtKB: U2 small nuclear ribonucleoprotein B''

Macromolecule #19: Small nuclear ribonucleoprotein F

• Macromolecule: Name: Small nuclear ribonucleoprotein F / type: protein_or_peptide / ID: 19 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.734171 KDa

Sequence

String:

MSLPLNPKPF LNGLTGKPVM VKLKWGMEYK GYLVSVDGYM NMQLANTEEY IDGALSGHLG EVLIRCNNVL YIRGVEEEEE DGEMRE

UniProtKB: Small nuclear ribonucleoprotein F

Macromolecule #20: Small nuclear ribonucleoprotein-associated proteins B and B'

• Macromolecule: Name: Small nuclear ribonucleoprotein-associated proteins B and B'; type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 24.642131 KDa

Sequence

String:

MTVGKSSKML QHIDYRMRCI LQDGRIFIGT FKAFDKHMNL ILCDCDEFRK IKPKNSKQAE REEKRVLGLV LLRGENLVSM TVEGPPPKD TGIARVPLAG AAGGPGIGRA AGRGIPAGVP MPQAPAGLAG PVRGVGGPSQ QVMTPQGRGT VAAAAAAATA S IAGAPTQY PPGRGGPPPP MGRGAPPPGM MGPPPGMRPP MGPPMGIPPG RGTPMGMPPP GMRPPPPGMR GPPPPGMRPP RP

UniProtKB: Small nuclear ribonucleoprotein-associated proteins B and B'

Macromolecule #21: Small nuclear ribonucleoprotein Sm D3

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D3 / type: protein_or_peptide / ID: 21 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.940308 KDa

Sequence

String:

MSIGVPIKVL HEAEGHIVTC ETNTGEVYRG KLIEAEDNMN CQMSNITVTY RDGRVAQLEQ VYIRGSKIRF LILPDMLKNA PMLKSMKNK NQGSGAGRGK AAILKAQVAA RGRGRGMGRG NIFQKRR

UniProtKB: Small nuclear ribonucleoprotein Sm D3

Macromolecule #22: Small nuclear ribonucleoprotein G

• Macromolecule: Name: Small nuclear ribonucleoprotein G / type: protein_or_peptide / ID: 22 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 8.508084 KDa

Sequence

String:

MSKAHPPELK KFMDKKLSLK LNGGRHVQGI LRGFDPFMNL VIDECVEMAT SGQQNNIGMV VIRGNSIIML EALERV

UniProtKB: Small nuclear ribonucleoprotein G

Macromolecule #23: Small nuclear ribonucleoprotein E

• Macromolecule: Name: Small nuclear ribonucleoprotein E / type: protein_or_peptide / ID: 23 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.817601 KDa

Sequence

String:

MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQS VSN

UniProtKB: Small nuclear ribonucleoprotein E

Macromolecule #24: Small nuclear ribonucleoprotein Sm D1

• Macromolecule: Name: Small nuclear ribonucleoprotein Sm D1 / type: protein_or_peptide / ID: 24 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 13.310653 KDa

Sequence

String:

MKLVRFLMKL SHETVTIELK NGTQVHGTIT GVDVSMNTHL KAVKMTLKNR EPVQLETLSI RGNNIRYFIL PDSLPLDTLL VDVEPKVKS KKREAVAGRG RGRGRGRGRG RGRGRGGPRR

UniProtKB: Small nuclear ribonucleoprotein Sm D1

Macromolecule #25: U2 small nuclear ribonucleoprotein A'

• Macromolecule: Name: U2 small nuclear ribonucleoprotein A' / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 28.456584 KDa

Sequence

String:

MVKLTAELIE QAAQYTNAVR DRELDLRGYK IPVIENLGAT LDQFDAIDFS DNEIRKLDGF PLLRRLKTLL VNNNRICRIG EGLDQALPC LTELILTNNS LVELGDLDPL ASLKSLTYLS ILRNPVTNKK HYRLYVIYKV PQVRVLDFQK VKLKERQEAE K MFKGKRGA QLAKDIARRS KTFNPGAGLP TDKKKGGPSP GDVEAIKNAI ANASTLAEVE RLKGLLQSGQ IPGRERRSGP TD DGEEEME EDTVTNGS

UniProtKB: U2 small nuclear ribonucleoprotein A'

Macromolecule #26: Splicing factor 3B subunit 4

• Macromolecule: Name: Splicing factor 3B subunit 4 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 44.43657 KDa

Sequence

String:

MAAGPISERN QDATVYVGGL DEKVSEPLLW ELFLQAGPVV NTHMPKDRVT GQHQGYGFVE FLSEEDADYA IKIMNMIKLY GKPIRVNKA SAHNKNLDVG ANIFIGNLDP EIDEKLLYDT FSAFGVILQT PKIMRDPDTG NSKGYAFINF ASFDASDAAI E AMNGQYLC NRPITVSYAF KKDSKGERHG SAAERLLAAQ NPLSQADRPH QLFADAPPPP SAPNPVVSSL GSGLPPPGMP PP GSFPPPV PPPGALPPGI PPAMPPPPMP PGAAGHGPPS AGTPGAGHPG HGHSHPHPFP PGGMPHPGMS QMQLAHHGPH GLG HPHAGP PGSGGQPPPR PPPGMPHPGP PPMGMPPRGP PFGSPMGHPG PMPPHGMRGP PPLMPPHGYT GPPRPPPYGY QRGP LPPPR PTPRPPVPPR GPLRGPLPQ

UniProtKB: Splicing factor 3B subunit 4

Macromolecule #27: Splicing factor 3A subunit 3

• Macromolecule: Name: Splicing factor 3A subunit 3 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 58.934844 KDa

Sequence

String:

METILEQQRR YHEEKERLMD VMAKEMLTKK STLRDQINSD HRTRAMQDRY MEVSGNLRDL YDDKDGLRKE ELNAISGPNE FAEFYNRLK QIKEFHRKHP NEICVPMSVE FEELLKAREN PSEEAQNLVE FTDEEGYGRY LDLHDCYLKY INLKASEKLD Y ITYLSIFD QLFDIPKERK NAEYKRYLEM LLEYLQDYTD RVKPLQDQNE LFGKIQAEFE KKWENGTFPG WPKETSSALT HA GAHLDLS AFSSWEELAS LGLDRLKSAL LALGLKCGGT LEERAQRLFS TKGKSLESLD TSLFAKNPKS KGTKRDTERN KDI AFLEAQ IYEYVEILGE QRHLTHENVQ RKQARTGEER EEEEEEQISE SESEDEENEI IYNPKNLPLG WDGKPIPYWL YKLH GLNIN YNCEICGNYT YRGPKAFQRH FAEWRHAHGM RCLGIPNTAH FANVTQIEDA VSLWAKLKLQ KASERWQPDT EEEYE DSSG NVVNKKTYED LKRQGLL

UniProtKB: Splicing factor 3A subunit 3

Macromolecule #28: Splicing factor 3A subunit 2

• Macromolecule: Name: Splicing factor 3A subunit 2 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 49.327355 KDa

Sequence

String:

MDFQHRPGGK TGSGGVASSS ESNRDRRERL RQLALETIDI NKDPYFMKNH LGSYECKLCL TLHNNEGSYL AHTQGKKHQT NLARRAAKE AKEAPAQPAP EKVKVEVKKF VKIGRPGYKV TKQRDSEMGQ QSLLFQIDYP EIAEGIMPRH RFMSAYEQRI E PPDRRWQY LLMAAEPYET IAFKVPSREI DKAEGKFWTH WNRETKQFFL QFHFKMEKPP APPSLPAGPP GVKRPPPPLM NG LPPRPPL PESLPPPPPG GLPLPPMPPT GPAPSGPPGP PQLPPPAPGV HPPAPVVHPP ASGVHPPAPG VHPPAPGVHP PAP GVHPPT SGVHPPAPGV HPPAPGVHPP APGVHPPAPG VHPPAPGVHP PPSAGVHPQA PGVHPAAPAV HPQAPGVHPP APGM HPQAP GVHPQPPGVH PSAPGVHPQP PGVHPSNPGV HPPTPMPPML RPPLPSEGPG NIPPPPPTN

UniProtKB: Splicing factor 3A subunit 2

Macromolecule #29: Splicing factor 3B subunit 2

• Macromolecule: Name: Splicing factor 3B subunit 2 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 100.377812 KDa

Sequence

String:

MATEHPEPPK AELQLPPPPP PGHYGAWAAQ ELQAKLAEIG APIQGNREEL VERLQSYTRQ TGIVLNRPVL RGEDGDKAAP PPMSAQLPG IPMPPPPLGL PPLQPPPPPP PPPPGLGLGF PMAHPPNLGP PPPLRVGEPV ALSEEERLKL AQQQAALLMQ Q EERAKQQG DHSLKEHELL EQQKRAAVLL EQERQQEIAK MGTPVPRPPQ DMGQIGVRTP LGPRVAAPVG PVGPTPTVLP MG APVPRPR GPPPPPGDEN REMDDPSVGP KIPQALEKIL QLKESRQEEM NSQQEEEEME TDARSSLGQS ASETEEDTVS VSK KEKNRK RRNRKKKKKP QRVRGVSSES SGDREKDSTR SRGSDSPAAD VEIEYVTEEP EIYEPNFIFF KRIFEAFKLT DDVK KEKEK EPEKLDKLEN SAAPKKKGFE EEHKDSDDDS SDDEQEKKPE APKLSKKKLR RMNRFTVAEL KQLVARPDVV EMHDV TAQD PKLLVHLKAT RNSVPVPRHW CFKRKYLQGK RGIEKPPFEL PDFIKRTGIQ EMREALQEKE EQKTMKSKMR EKVRPK MGK IDIDYQKLHD AFFKWQTKPK LTIHGDLYYE GKEFETRLKE KKPGDLSDEL RISLGMPVGP NAHKVPPPWL IAMQRYG PP PSYPNLKIPG LNSPIPESCS FGYHAGGWGK PPVDETGKPL YGDVFGTNAA EFQTKTEEEE IDRTPWGELE PSDEESSE E EEEEESDEDK PDETGFITPA DSGLITPGGF SSVPAGMETP ELIELRKKKI EEAMDGSETP QLFTVLPEKR TATVGGAMM GSTHIYDMST VMSRKGPAPE LQGVEVALAP EELELDPMAM TQKYEEHVRE QQAQVEKEDF SDMVAEHAAK QKQKKRKAQP QDSRGGSKK YKEFKF

UniProtKB: Splicing factor 3B subunit 2

Macromolecule #30: Splicing factor 3B subunit 5

• Macromolecule: Name: Splicing factor 3B subunit 5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.149369 KDa

Sequence

String:

MTDRYTIHSQ LEHLQSKYIG TGHADTTKWE WLVNQHRDSY CSYMGHFDLL NYFAIAENES KARVRFNLME KMLQPCGPPA DKPEEN

UniProtKB: Splicing factor 3B subunit 5

Macromolecule #31: Splicing factor 3B subunit 3

• Macromolecule: Name: Splicing factor 3B subunit 3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 135.718844 KDa

Sequence

String:

MFLYNLTLQR ATGISFAIHG NFSGTKQQEI VVSRGKILEL LRPDPNTGKV HTLLTVEVFG VIRSLMAFRL TGGTKDYIVV GSDSGRIVI LEYQPSKNMF EKIHQETFGK SGCRRIVPGQ FLAVDPKGRA VMISAIEKQK LVYILNRDAA ARLTISSPLE A HKANTLVY HVVGVDVGFE NPMFACLEMD YEEADNDPTG EAAANTQQTL TFYELDLGLN HVVRKYSEPL EEHGNFLITV PG GSDGPSG VLICSENYIT YKNFGDQPDI RCPIPRRRND LDDPERGMIF VCSATHKTKS MFFFLAQTEQ GDIFKITLET DED MVTEIR LKYFDTVPVA AAMCVLKTGF LFVASEFGNH YLYQIAHLGD DDEEPEFSSA MPLEEGDTFF FQPRPLKNLV LVDE LDSLS PILFCQIADL ANEDTPQLYV ACGRGPRSSL RVLRHGLEVS EMAVSELPGN PNAVWTVRRH IEDEFDAYII VSFVN ATLV LSIGETVEEV TDSGFLGTTP TLSCSLLGDD ALVQVYPDGI RHIRADKRVN EWKTPGKKTI VKCAVNQRQV VIALTG GEL VYFEMDPSGQ LNEYTERKEM SADVVCMSLA NVPPGEQRSR FLAVGLVDNT VRIISLDPSD CLQPLSMQAL PAQPESL CI VEMGGTEKQD ELGERGSIGF LYLNIGLQNG VLLRTVLDPV TGDLSDTRTR YLGSRPVKLF RVRMQGQEAV LAMSSRSW L SYSYQSRFHL TPLSYETLEF ASGFASEQCP EGIVAISTNT LRILALEKLG AVFNQVAFPL QYTPRKFVIH PESNNLIII ETDHNAYTEA TKAQRKQQMA EEMVEAAGED ERELAAEMAA AFLNENLPES IFGAPKAGNG QWASVIRVMN PIQGNTLDLV QLEQNEAAF SVAVCRFSNT GEDWYVLVGV AKDLILNPRS VAGGFVYTYK LVNNGEKLEF LHKTPVEEVP AAIAPFQGRV L IGVGKLLR VYDLGKKKLL RKCENKHIAN YISGIQTIGH RVIVSDVQES FIWVRYKRNE NQLIIFADDT YPRWVTTASL LD YDTVAGA DKFGNICVVR LPPNTNDEVD EDPTGNKALW DRGLLNGASQ KAEVIMNYHV GETVLSLQKT TLIPGGSESL VYT TLSGGI GILVPFTSHE DHDFFQHVEM HLRSEHPPLC GRDHLSFRSY YFPVKNVIDG DLCEQFNSME PNKQKNVSEE LDRT PPEVS KKLEDIRTRY AF

UniProtKB: Splicing factor 3B subunit 3

Macromolecule #32: Splicing factor 3B subunit 1

• Macromolecule: Name: Splicing factor 3B subunit 1 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 146.024938 KDa

Sequence

String:

MAKIAKTHED IEAQIREIQG KKAALDEAQG VGLDSTGYYD QEIYGGSDSR FAGYVTSIAA TELEDDDDDY SSSTSLLGQK KPGYHAPVA LLNDIPQSTE QYDPFAEHRP PKIADREDEY KKHRRTMIIS PERLDPFADG GKTPDPKMNA RTYMDVMREQ H LTKEEREI RQQLAEKAKA GELKVVNGAA ASQPPSKRKR RWDQTADQTP GATPKKLSSW DQAETPGHTP SLRWDETPGR AK GSETPGA TPGSKIWDPT PSHTPAGAAT PGRGDTPGHA TPGHGGATSS ARKNRWDETP KTERDTPGHG SGWAETPRTD RGG DSIGET PTPGASKRKS RWDETPASQM GGSTPVLTPG KTPIGTPAMN MATPTPGHIM SMTPEQLQAW RWEREIDERN RPLS DEELD AMFPEGYKVL PPPAGYVPIR TPARKLTATP TPLGGMTGFH MQTEDRTMKS VNDQPSGNLP FLKPDDIQYF DKLLV DVDE STLSPEEQKE RKIMKLLLKI KNGTPPMRKA ALRQITDKAR EFGAGPLFNQ ILPLLMSPTL EDQERHLLVK VIDRIL YKL DDLVRPYVHK ILVVIEPLLI DEDYYARVEG REIISNLAKA AGLATMISTM RPDIDNMDEY VRNTTARAFA VVASALG IP SLLPFLKAVC KSKKSWQARH TGIKIVQQIA ILMGCAILPH LRSLVEIIEH GLVDEQQKVR TISALAIAAL AEAATPYG I ESFDSVLKPL WKGIRQHRGK GLAAFLKAIG YLIPLMDAEY ANYYTREVML ILIREFQSPD EEMKKIVLKV VKQCCGTDG VEANYIKTEI LPPFFKHFWQ HRMALDRRNY RQLVDTTVEL ANKVGAAEII SRIVDDLKDE AEQYRKMVME TIEKIMGNLG AADIDHKLE EQLIDGILYA FQEQTTEDSV MLNGFGTVVN ALGKRVKPYL PQICGTVLWR LNNKSAKVRQ QAADLISRTA V VMKTCQEE KLMGHLGVVL YEYLGEEYPE VLGSILGALK AIVNVIGMHK MTPPIKDLLP RLTPILKNRH EKVQENCIDL VG RIADRGA EYVSAREWMR ICFELLELLK AHKKAIRRAT VNTFGYIAKA IGPHDVLATL LNNLKVQERQ NRVCTTVAIA IVA ETCSPF TVLPALMNEY RVPELNVQNG VLKSLSFLFE YIGEMGKDYI YAVTPLLEDA LMDRDLVHRQ TASAVVQHMS LGVY GFGCE DSLNHLLNYV WPNVFETSPH VIQAVMGALE GLRVAIGPCR MLQYCLQGLF HPARKVRDVY WKIYNSIYIG SQDAL IAHY PRIYNDDKNT YIRYELDYIL

UniProtKB: Splicing factor 3B subunit 1

Macromolecule #33: Splicing factor 3B subunit 6

• Macromolecule: Name: Splicing factor 3B subunit 6 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 14.6069 KDa

Sequence

String:

MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVV LYYNANRAFQ KMDTKKKEEQ LKLLKEKYGI NTDPPK

UniProtKB: Splicing factor 3B subunit 6

Macromolecule #34: Beta-catenin-like protein 1

• Macromolecule: Name: Beta-catenin-like protein 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.26434 KDa

Sequence

String:

MDVGELLSYQ PNRGTKRPRD DEEEEQKMRR KQTGTRERGR YREEEMTVVE EADDDKKRLL QIIDRDGEEE EEEEEPLDES SVKKMILTF EKRSYKNQEL RIKFPDNPEK FMESELDLND IIQEMHVVAT MPDLYHLLVE LNAVQSLLGL LGHDNTDVSI A VVDLLQEL TDIDTLHESE EGAEVLIDAL VDGQVVALLV QNLERLDESV KEEADGVHNT LAIVENMAEF RPEMCTEGAQ QG LLQWLLK RLKAKMPFDA NKLYCSEVLA ILLQDNDENR ELLGELDGID VLLQQLSVFK RHNPSTAEEQ EMMENLFDSL CSC LMLSSN RERFLKGEGL QLMNLMLREK KISRSSALKV LDHAMIGPEG TDNCHKFVDI LGLRTIFPLF MKSPRKIKKV GTTE KEHEE HVCSILASLL RNLRGQQRTR LLNKFTENDS EKVDRLMELH FKYLGAMQVA DKKIEGEKHD MVRRGEIIDN DTEEE FYLR RLDAGLFVLQ HICYIMAEIC NANVPQIRQR VHQILNMRGS SIKIVRHIIK EYAENIGDGR SPEFRENEQK RILGLL ENF

UniProtKB: Beta-catenin-like protein 1

Macromolecule #35: U2 snRNP-associated SURP motif-containing protein

• Macromolecule: Name: U2 snRNP-associated SURP motif-containing protein / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 118.503312 KDa

Sequence

String:

MADKTPGGSQ KASSKTRSSD VHSSGSSDAH MDASGPSDSD MPSRTRPKSP RKHNYRNESA RESLCDSPHQ NLSRPLLENK LKAFSIGKM STAKRTLSKK EQEELKKKED EKAAAEIYEE FLAAFEGSDG NKVKTFVRGG VVNAAKEEHE TDEKRGKIYK P SSRFADQK NPPNQSSNER PPSLLVIETK KPPLKKGEKE KKKSNLELFK EELKQIQEER DERHKTKGRL SRFEPPQSDS DG QRRSMDA PSRRNRSSGV LDDYAPGSHD VGDPSTTNLY LGNINPQMNE EMLCQEFGRF GPLASVKIMW PRTDEERARE RNC GFVAFM NRRDAERALK NLNGKMIMSF EMKLGWGKAV PIPPHPIYIP PSMMEHTLPP PPSGLPFNAQ PRERLKNPNA PMLP PPKNK EDFEKTLSQA IVKVVIPTER NLLALIHRMI EFVVREGPMF EAMIMNREIN NPMFRFLFEN QTPAHVYYRW KLYSI LQGD SPTKWRTEDF RMFKNGSFWR PPPLNPYLHG MSEEQETEAF VEEPSKKGAL KEEQRDKLEE ILRGLTPRKN DIGDAM VFC LNNAEAAEEI VDCITESLSI LKTPLPKKIA RLYLVSDVLY NSSAKVANAS YYRKFFETKL CQIFSDLNAT YRTIQGH LQ SENFKQRVMT CFRAWEDWAI YPEPFLIKLQ NIFLGLVNII EEKETEDVPD DLDGAPIEEE LDGAPLEDVD GIPIDATP I DDLDGVPIKS LDDDLDGVPL DATEDSKKNE PIFKVAPSKW EAVDESELEA QAVTTSKWEL FDQHEESEEE ENQNQEEES EDEEDTQSSK SEEHHLYSNP IKEEMTESKF SKYSEMSEEK RAKLREIELK VMKFQDELES GKRPKKPGQS FQEQVEHYRD KLLQREKEK ELERERERDK KDKEKLESRS KDKKEKDECT PTRKERKRRH STSPSPSRSS SGRRVKSPSP KSERSERSER S HKESSRSR SSHKDSPRDV SKKAKRSPSG SRTPKRSRRS RSRSPKKSGK KSRSQSRSPH RSHKKSKKNK H

UniProtKB: U2 snRNP-associated SURP motif-containing protein

Macromolecule #36: ATP-dependent RNA helicase DHX15

• Macromolecule: Name: ATP-dependent RNA helicase DHX15 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 91.065805 KDa

Sequence

String:

MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSA HSTHSTHSAH STHAGHAGHT SLPQCINPFT NLPHTPRYYD ILKKRLQLPV WEYKDRFTDI LVRHQSFVLV G ETGSGKTT QIPQWCVEYM RSLPGPKRGV ACTQPRRVAA MSVAQRVADE MDVMLGQEVG YSIRFEDCSS AKTILKYMTD GM LLREAMN DPLLERYGVI ILDEAHERTL ATDILMGVLK EVVRQRSDLK VIVMSATLDA GKFQIYFDNC PLLTIPGRTH PVE IFYTPE PERDYLEAAI RTVIQIHMCE EEEGDLLLFL TGQEEIDEAC KRIKREVDDL GPEVGDIKII PLYSTLPPQQ QQRI FEPPP PKKQNGAIGR KVVVSTNIAE TSLTIDGVVF VIDPGFAKQK VYNPRIRVES LLVTAISKAS AQQRAGRAGR TRPGK CFRL YTEKAYKTEM QDNTYPEILR SNLGSVVLQL KKLGIDDLVH FDFMDPPAPE TLMRALELLN YLAALNDDGD LTELGS MMA EFPLDPQLAK MVIASCDYNC SNEVLSITAM LSVPQCFVRP TEAKKAADEA KMRFAHIDGD HLTLLNVYHA FKQNHES VQ WCYDNFINYR SLMSADNVRQ QLSRIMDRFN LPRRSTDFTS RDYYINIRKA LVTGYFMQVA HLERTGHYLT VKDNQVVQ L HPSTVLDHKP EWVLYNEFVL TTKNYIRTCT DIKPEWLVKI APQYYDMSNF PQCEAKRQLD RIIAKLQSKE YSQY

UniProtKB: ATP-dependent RNA helicase DHX15

Macromolecule #38: Protein Red

• Macromolecule: Name: Protein Red / type: protein_or_peptide / ID: 38 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.731508 KDa

Sequence

String:

MPERDSEPFS NPLAPDGHDV DDPHSFHQSK LTNEDFRKLL MTPRAAPTSA PPSKSRHHEM PREYNEDEDP AARRRKKKSY YAKLRQQEI ERERELAEKY RDRAKERRDG VNKDYEETEL ISTTANYRAV GPTAEADKSA AEKRRQLIQE SKFLGGDMEH T HLVKGLDF ALLQKVRAEI ASKEKEEEEL MEKPQKETKK DEDPENKIEF KTRLGRNVYR MLFKSKAYER NELFLPGRMA YV VDLDDEY ADTDIPTTLI RSKADCPTME AQTTLTTNDI VISKLTQILS YLRQGTRNKK LKKKDKGKLE EKKPPEADMN IFE DIGDYV PSTTKTPRDK ERERYRERER DRERDRDRDR ERERERDRER ERERDREREE EKKRHSYFEK PKVDDEPMDV DKGP GSTKE LIKSINEKFA GSAGWEGTES LKKPEDKKQL GDFFGMSNSY AECYPATMDD MAVDSDEEVD YSKMDQGNKK GPLGR WDFD TQEEYSEYMN NKEALPKAAF QYGIKMSEGR KTRRFKETND KAELDRQWKK ISAIIEKRKK MEADGVEVKR PKY

UniProtKB: Protein Red

Macromolecule #39: WD40 repeat-containing protein SMU1

• Macromolecule: Name: WD40 repeat-containing protein SMU1 / type: protein_or_peptide / ID: 39 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 57.616742 KDa

Sequence

String:

MSIEIESSDV IRLIMQYLKE NSLHRALATL QEETTVSLNT VDSIESFVAD INSGHWDTVL QAIQSLKLPD KTLIDLYEQV VLELIELRE LGAARSLLRQ TDPMIMLKQT QPERYIHLEN LLARSYFDPR EAYPDGSSKE KRRAAIAQAL AGEVSVVPPS R LMALLGQA LKWQQHQGLL PPGMTIDLFR GKAAVKDVEE EKFPTQLSRH IKFGQKSHVE CARFSPDGQY LVTGSVDGFI EV WNFTTGK IRKDLKYQAQ DNFMMMDDAV LCMCFSRDTE MLATGAQDGK IKVWKIQSGQ CLRRFERAHS KGVTCLSFSK DSS QILSAS FDQTIRIHGL KSGKTLKEFR GHSSFVNEAT FTQDGHYIIS ASSDGTVKIW NMKTTECSNT FKSLGSTAGT DITV NSVIL LPKNPEHFVV CNRSNTVVIM NMQGQIVRSF SSGKREGGDF VCCALSPRGE WIYCVGEDFV LYCFSTVTGK LERTL TVHE KDVIGIAHHP HQNLIATYSE DGLLKLWKP

UniProtKB: WD40 repeat-containing protein SMU1

Macromolecule #40: Pre-mRNA-splicing factor RBM22

• Macromolecule: Name: Pre-mRNA-splicing factor RBM22 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 56.285566 KDa

Sequence

String:

EDADFPILCQ TCLGENPYIR MTKEKYGKEC KICARPFTVF RWCPGVRMRF KKTEVCQTCS KLKNVCQTCL LDLEYGLPIQ MATSLGSNT YNRQNWEDAD FPILCQTCLG ENPYIRMTKE KYGKECKICA RPFTVFRWCP GVRMRFKKTE VCQTCSKLKN V CQTCLLDL EYGLPIQVRD AGLSFKDDMP KSDVNKEYYT QNMEREISNS DGTRPVGMLG KATSTSDMLL KLARTTPYYK RN RPHICSF WVKGECKRGE ECPYRHEKPT DPDDPLADQN IKDRYYGIND PVADKLLKRA STMPRLDPPE DKTITTLYVG GLG DTITET DLRNHFYQFG EIRTITVVQR QQCAFIQFAT RQAAEVAAEK SFNKLIVNGR RLNVKWGRSQ AARGKEKEKD GTTD SGIKL EPVPGLPGAL PPPPAAEEEA SANYFNLPPS GPPAVVNIAL PPPPGIAPPP PPGFGPHMFH PMGPPPPFMR APGPI HYPS QDPQRMGAHA GKHSSP

Macromolecule #41: RING-type E3 ubiquitin-protein ligase PPIL2

• Macromolecule: Name: RING-type E3 ubiquitin-protein ligase PPIL2 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO / EC number: RING-type E3 ubiquitin transferase
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 58.910379 KDa

Sequence

String:

MGKRQHQKDK MYITCAEYTH FYGGKKPDLP QTNFRRLPFD HCSLSLQPFV YPVCTPDGIV FDLLNIVPWL KKYGTNPSNG EKLDGRSLI KLNFSKNSEG KYHCPVLFTV FTNNTHIVAV RTTGNVYAYE AVEQLNIKAK NFRDLLTDEP FSRQDIITLQ D PTNLDKFN VSNFYHVKNN MKIIDPDEEK AKQDPSYYLK NTNAETRETL QELYKEFKGD EILAATMKAP EKKKVDKLNA AH YSTGKVS ASFTSTAMVP ETTHEAAAID EDVLRYQFVK KKGYVRLHTN KGDLNLELHC DLTPKTCENF IRLCKKHYYD GTI FHRSIR NFVIQGGDPT GTGTGGESYW GKPFKDEFRP NLSHTGRGIL SMANSGPNSN RSQFFITFRS CAYLDKKHTI FGRV VGGFD VLTAMENVES DPKTDRPKEE IRIDATTVFV DPYEEADAQI AQERKTQLKV APETKVKSSQ PQAGSQGPQT FRQGV GKYI NPAATKRAAE EEPSTSATVP MSKKKPSRGF GDFSSW

UniProtKB: RING-type E3 ubiquitin-protein ligase PPIL2

Macromolecule #42: U6 snRNA-associated Sm-like protein LSm2

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm2 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.847495 KDa

Sequence

String:

MLFYSFFKSL VGKDVVVELK NDLSICGTLH SVDQYLNIKL TDISVTDPEK YPHMLSVKNC FIRGSVVRYV QLPADEVDTQ LLQDAARKE ALQQKQ

UniProtKB: U6 snRNA-associated Sm-like protein LSm2

Macromolecule #43: U6 snRNA-associated Sm-like protein LSm3

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm3 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.85939 KDa

Sequence

String:

MADDVDQQQT TNTVEEPLDL IRLSLDERIY VKMRNDRELR GRLHAYDQHL NMILGDVEET VTTIEIDEET YEEIYKSTKR NIPMLFVRG DGVVLVAPPL RVG

UniProtKB: U6 snRNA-associated Sm-like protein LSm3

Macromolecule #44: U6 snRNA-associated Sm-like protein LSm4

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm4 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 15.375775 KDa

Sequence

String:

MLPLSLLKTA QNHPMLVELK NGETYNGHLV SCDNWMNINL REVICTSRDG DKFWRMPECY IRGSTIKYLR IPDEIIDMVK EEVVAKGRG RGGLQQQKQQ KGRGMGGAGR GVFGGRGRGG IPGTGRGQPE KKPGRQAGKQ

UniProtKB: U6 snRNA-associated Sm-like protein LSm4

Macromolecule #45: U6 snRNA-associated Sm-like protein LSm5

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm5 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.945448 KDa

Sequence

String:

MAANATTNPS QLLPLELVDK CIGSRIHIVM KSDKEIVGTL LGFDDFVNMV LEDVTEFEIT PEGRRITKLD QILLNGNNIT MLVPGGEGP EV

UniProtKB: U6 snRNA-associated Sm-like protein LSm5

Macromolecule #46: U6 snRNA-associated Sm-like protein LSm6

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm6 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 9.139571 KDa

Sequence

String:

MSLRKQTPSD FLKQIIGRPV VVKLNSGVDY RGVLACLDGY MNIALEQTEE YVNGQLKNKY GDAFIRGNNV LYISTQKRRM

UniProtKB: U6 snRNA-associated Sm-like protein LSm6

Macromolecule #47: U6 snRNA-associated Sm-like protein LSm7

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm7 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 11.617363 KDa

Sequence

String:

MADKEKKKKE SILDLSKYID KTIRVKFQGG REASGILKGF DPLLNLVLDG TIEYMRDPDD QYKLTEDTRQ LGLVVCRGTS VVLICPQDG MEAIPNPFIQ QQDA

UniProtKB: U6 snRNA-associated Sm-like protein LSm7

Macromolecule #48: U6 snRNA-associated Sm-like protein LSm8

• Macromolecule: Name: U6 snRNA-associated Sm-like protein LSm8 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 10.410589 KDa

Sequence

String:

MTSALENYIN RTVAVITSDG RMIVGTLKGF DQTINLILDE SHERVFSSSQ GVEQVVLGLY IVRGDNVAVI GEIDEETDSA LDLGNIRAE PLNSVAH

UniProtKB: U6 snRNA-associated Sm-like protein LSm8

Macromolecule #49: SNW domain-containing protein 1

• Macromolecule: Name: SNW domain-containing protein 1 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 61.610703 KDa

Sequence

String:

MALTSFLPAP TQLSQDQLEA EEKARSQRSR QTSLVSSRRE PPPYGYRKGW IPRLLEDFGD GGAFPEIHVA QYPLDMGRKK KMSNALAIQ VDSEGKIKYD AIARQGQSKD KVIYSKYTDL VPKEVMNADD PDLQRPDEEA IKEITEKTRV ALEKSVSQKV A AAMPVRAA DKLAPAQYIR YTPSQQGVAF NSGAKQRVIR MVEMQKDPME PPRFKINKKI PRGPPSPPAP VMHSPSRKMT VK EQQEWKI PPCISNWKNA KGYTIPLDKR LAADGRGLQT VHINENFAKL AEALYIADRK AREAVEMRAQ VERKMAQKEK EKH EEKLRE MAQKARERRA GIKTHVEKED GEARERDEIR HDRRKERQHD RNLSRAAPDK RSKLQRNENR DISEVIALGV PNPR TSNEV QYDQRLFNQS KGMDSGFAGG EDEIYNVYDQ AWRGGKDMAQ SIYRPSKNLD KDMYGDDLEA RIKTNRFVPD KEFSG SDRR QRGREGPVQF EEDPFGLDKF LEEAKQHGGS KRPSDSSRPK EHEHEGKKRR KE

UniProtKB: SNW domain-containing protein 1

Macromolecule #50: Serine/arginine-rich splicing factor 1

• Macromolecule: Name: Serine/arginine-rich splicing factor 1 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 27.798752 KDa

Sequence

String:

MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFP RSGRGTGRGG GGGGGGGAPR GRYGPPSRRS ENRVVVSGLP PSGSWQDLKD HMREAGDVCY ADVYRDGTGV V EFVRKEDM TYAVRKLDNT KFRSHEGETA YIRVKVDGPR SPSYGRSRSR SRSRSRSRSR SNSRSRSYSP RRSRGSPRYS PR HSRSRSR T

UniProtKB: Serine/arginine-rich splicing factor 1

Macromolecule #51: Transcription elongation regulator 1

• Macromolecule: Name: Transcription elongation regulator 1 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 124.083 KDa

Sequence

String:

MAERGGDGGE SERFNPGELR MAQQQALRFR GPAPPPNAVM RGPPPLMRPP PPFGMMRGPP PPPRPPFGRP PFDPNMPPMP PPGGIPPPM GPPHLQRPPF MPPPMSSMPP PPGMMFPPGM PPVTAPGTPA LPPTEEIWVE NKTPDGKVYY YNARTRESAW T KPDGVKVI QQSELTPMLA AQAQVQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQ AQVQAQV QAQVQAQAVG ASTPTTSSPA PAVSTSTSSS TPSSTTSTTT TATSVAQTVS TPTTQDQTPS SAVSVATPTV SVS TPAPTA TPVQTVPQPH PQTLPPAVPH SVPQPTTAIP AFPPVMVPPF RVPLPGMPIP LPGVAMMQIV SCPYVKTVAT TKTG VLPGM APPIVPMIHP QVAIAASPAT LAGATAVSEW TEYKTADGKT YYYNNRTLES TWEKPQELKE KEKLEEKIKE PIKEP SEEP LPMETEEEDP KEEPIKEIKE EPKEEEMTEE EKAAQKAKPV ATAPIPGTPW CVVWTGDERV FFYNPTTRLS MWDRPD DLI GRADVDKIIQ EPPHKKGMEE LKKLRHPTPT MLSIQKWQFS MSAIKEEQEL MEEINEDEPV KAKKRKRDDN KDIDSEK EA AMEAEIKAAR ERAIVPLEAR MKQFKDMLLE RGVSAFSTWE KELHKIVFDP RYLLLNPKER KQVFDQYVKT RAEEERRE K KNKIMQAKED FKKMMEEAKF NPRATFSEFA AKHAKDSRFK AIEKMKDREA LFNEFVAAAR KKEKEDSKTR GEKIKSDFF ELLSNHHLDS QSRWSKVKDK VESDPRYKAV DSSSMREDLF KQYIEKIAKN LDSEKEKELE RQARIEASLR EREREVQKAR SEQTKEIDR EREQHKREEA IQNFKALLSD MVRSSDVSWS DTRRTLRKDH RWESGSLLER EEKEKLFNEH IEALTKKKRE H FRQLLDET SAITLTSTWK EVKKIIKEDP RCIKFSSSDR KKQREFEEYI RDKYITAKAD FRTLLKETKF ITYRSKKLIQ ES DQHLKDV EKILQNDKRY LVLDCVPEER RKLIVAYVDD LDRRGPPPPP TASEPTRRST K

UniProtKB: Transcription elongation regulator 1

Macromolecule #52: Microfibrillar-associated protein 1

• Macromolecule: Name: Microfibrillar-associated protein 1 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 52.050527 KDa

Sequence

String:

MSVPSALMKQ PPIQSTAGAV PVRNEKGEIS MEKVKVKRYV SGKRPDYAPM ESSDEEDEEF QFIKKAKEQE AEPEEQEEDS SSDPRLRRL QNRISEDVEE RLARHRKIVE PEVVGESDSE VEGDAWRMER EDSSEEEEEE IDDEEIERRR GMMRQRAQER K NEEMEVME VEDEGRSGEE SESESEYEEY TDSEDEMEPR LKPVFIRKKD RVTVQEREAE ALKQKELEQE AKRMAEERRK YT LKIVEEE TKKELEENKR SLAALDALNT DDENDEEEYE AWKVRELKRI KRDREDREAL EKEKAEIERM RNLTEEERRA ELR ANGKVI TNKAVKGKYK FLQKYYHRGA FFMDEDEEVY KRDFSAPTLE DHFNKTILPK VMQVKNFGRS GRTKYTHLVD QDTT SFDSA WGQESAQNTK FFKQKAAGVR DVFERPSAKK RKTT

UniProtKB: Microfibrillar-associated protein 1

Macromolecule #53: Pre-mRNA-processing factor 17

• Macromolecule: Name: Pre-mRNA-processing factor 17 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 65.61218 KDa

Sequence

String:

MSAAIAALAA SYGSGSGSES DSDSESSRCP LPAADSLMHL TKSPSSKPSL AVAVDSAPEV AVKEDLETGV HLDPAVKEVQ YNPTYETMF APEFGPENPF RTQQMAAPRN MLSGYAEPAH INDFMFEQQR RTFATYGYAL DPSLDNHQVS AKYIGSVEEA E KNQGLTVF ETGQKKTEKR KKFKENDASN IDGFLGPWAK YVDEKDVAKP SEEEQKELDE ITAKRQKKGK QEEEKPGEEK TI LHVKEMY DYQGRSYLHI PQDVGVNLRS TMPPEKCYLP KKQIHVWSGH TKGVSAVRLF PLSGHLLLSC SMDCKIKLWE VYG ERRCLR TFIGHSKAVR DICFNTAGTQ FLSAAYDRYL KLWDTETGQC ISRFTNRKVP YCVKFNPDED KQNLFVAGMS DKKI VQWDI RSGEIVQEYD RHLGAVNTIV FVDENRRFVS TSDDKSLRVW EWDIPVDFKY IAEPSMHSMP AVTLSPNGKW LACQS MDNQ ILIFGAQNRF RLNKKKIFKG HMVAGYACQV DFSPDMSYVI SGDGNGKLNI WDWKTTKLYS RFKAHDKVCI GAVWHP HET SKVITCGWDG LIKLWD

UniProtKB: Pre-mRNA-processing factor 17

Macromolecule #54: PHD finger-like domain-containing protein 5A

• Macromolecule: Name: PHD finger-like domain-containing protein 5A / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 12.427524 KDa

Sequence

String:

MAKHHPDLIF CRKQAGVAIG RLCEKCDGKC VICDSYVRPC TLVRICDECN YGSYQGRCVI CGGPGVSDAY YCKECTIQEK DRDGCPKIV NLGSSKTDLF YERKKYGFKK R

UniProtKB: PHD finger-like domain-containing protein 5A

Macromolecule #3: U2 snRNA

• Macromolecule: Name: U2 snRNA / type: rna / ID: 3 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 60.186445 KDa

Sequence

String:

AUCGCUUCUC GGCCUUUUGG CUAAGAUCAA GUGUAGUAUC UGUUCUUAUC AGUUUAAUAU CUGAUACGUC CUCUAUCCGA GGACAAUAU AUUAAAUGGA UUUUUGGAGC AGGGAGAUGG AAUAGGAGCU UGCUCCGUCC ACUCCACGCA UCGACCUGGU A UUGCAGUA CCUCCAGGAA CGGUGCACCC

GENBANK: GENBANK: X02665.1

Macromolecule #4: U6 snRNA

• Macromolecule: Name: U6 snRNA / type: rna / ID: 4 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 34.09827 KDa

Sequence

String:

GUGCUCGCUU CGGCAGCACA UAUACUAAAA UUGGAACGAU ACAGAGAAGA UUAGCAUGGC CCCUGCGCAA GGAUGACACG CAAAUUCGU GAAGCGUUCC AUAUUUU

GENBANK: GENBANK: NR_004394.1

Macromolecule #6: U5 snRNA

• Macromolecule: Name: U5 snRNA / type: rna / ID: 6 / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 37.254855 KDa

Sequence

String:

AUACUCUGGU UUCUCUUCAG AUCGCAUAAA UCUUUCGCCU UUUACUAAAG AUUUCCGUGG AGAGGAACAA CUCUGAGUCU UAACCCAAU UUUUUGAGGC CUUGCUUUGG CAAGGCUA

GENBANK: GENBANK: AC105129.4

Macromolecule #37: pre-mRNA

• Macromolecule: Name: pre-mRNA / type: rna / ID: 37 / Details: PM5-10 pre-mRNA with MS2 loop / Number of copies: 1
• Source (natural): Organism: Homo sapiens (human)
• Molecular weight: Theoretical: 132.5945 KDa

Sequence

String:

GAAUACAAGC UCAUCCGAUA UCCGUACACC AUCAGGGUAC GAGCUAGCCC AUGGCGUACA CCAUCAGGGU ACGACUAGUA GAUCUCGUA CACCAUCAGG GUACGGAAUU CUCUAGAGUC GAGGAGGACA UCUCAGCAAA AGAGAAGCUG CUGCGGGCGU C GGAGGACG AGCGGGACCG GGUGCUGGAG GAGCUGCACA AGGCAGAGGA CAGCCUGCUG GCUGCCGACG AGACCGCCGC CA AGGUAUG UAUCAAGCUU ACAAGACAGC UUUAAGGAGA CCAAUAGAAA CUGGGCAUGU GGAGACAGAG AAGACUCUUG GCC UCGAGA AACCUGUAAC UGGAAUGUGU GUGGAGUGUG ACUGAUAGAA CACUACCUGA UUCUUUUGUA UUUACUGACC UGUG UUUUU UUGCU

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Buffer: pH: 7.9
• Vitrification: Cryogen name: ETHANE
• Details: OTS-stalled spliceosome complex particles

Electron microscopy

• Microscope: TFS TITAN THEMIS
• Image recording: Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 39.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: NONE / Details: ab initio
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 8.5 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 55345
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD