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- EMDB-53835: Cryo-EM structure of hSA3, a mutated hyper-soluble human serum al... -

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Basic information

Entry
Database: EMDB / ID: EMD-53835
TitleCryo-EM structure of hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression
Map data
Sample
  • Complex: hSA3
    • Protein or peptide: hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression
Keywordsligand and peptide binding protein / PROTEIN TRANSPORT
Biological speciessynthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.0 Å
AuthorsDe Felice S / Cendron L
Funding support Italy, 1 items
OrganizationGrant numberCountry
Italian Ministry of Health Italy
CitationJournal: To Be Published
Title: Cryo-EM structure of hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression, in complex with NbAlb1
Authors: De Felice S / Huber ST / Cendron L
History
DepositionMay 16, 2025-
Header (metadata) releaseMay 27, 2026-
Map releaseMay 27, 2026-
UpdateMay 27, 2026-
Current statusMay 27, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53835.png

Downloads & links

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Map

FileDownload / File: emd_53835.map.gz / Format: CCP4 / Size: 11.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.67 Å/pix.
x 144 pix.
= 240.768 Å		1.67 Å/pix.
x 144 pix.
= 240.768 Å		1.67 Å/pix.
x 144 pix.
= 240.768 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.672 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0289
Minimum - Maximum-0.06675833 - 0.30441445
Average (Standard dev.)0.0010841595 (±0.008615968)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions144144144
Spacing144144144
CellA=B=C: 240.768 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_53835_msk_1.map
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Additional map: #1

Fileemd_53835_additional_1.map
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Additional map: #2

Fileemd_53835_additional_2.map
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Half map: #1

Fileemd_53835_half_map_1.map
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Half map: #2

Fileemd_53835_half_map_2.map
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Sample components

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Entire : hSA3

EntireName: hSA3
Components
  • Complex: hSA3
    • Protein or peptide: hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression

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Supramolecule #1: hSA3

SupramoleculeName: hSA3 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 66 kDa/nm

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Macromolecule #1: hSA3, a mutated hyper-soluble human serum albumin designed for ba...

MacromoleculeName: hSA3, a mutated hyper-soluble human serum albumin designed for bacterial expression
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 68.510547 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: DAHKSEVAHR FKDLGEENFK ALVLIAFAQY LQKCPFEELV KMVKEVTEFA KKCVADETAE NCDKSLHTLF GDKLCQVATL RETYGEMAD CCAKQDPERH ECFLQHKDDN PNLPRLERPE PDVMCKAFHD NEETFLNKYL YEIARRHPYF YAPELLYFAK R YKKAFTEC ...String:
DAHKSEVAHR FKDLGEENFK ALVLIAFAQY LQKCPFEELV KMVKEVTEFA KKCVADETAE NCDKSLHTLF GDKLCQVATL RETYGEMAD CCAKQDPERH ECFLQHKDDN PNLPRLERPE PDVMCKAFHD NEETFLNKYL YEIARRHPYF YAPELLYFAK R YKKAFTEC CQAEDKAACL LPKLDALREE GKESSAKQRH KCAILQKFGE RAFKAWAIAR LSQRFPKAPF EEISKLVTDL TK VHKECCH GDMLECMDDR AKLAKYICEN QDSISSKLKE CCEKPLLERS HCIAEVENDD KPEDLPPLAA DFVESKDVCK NYA EAKDVF LARFLYEYAR RHPDYSVTLL LRIAKTYEDT LEKCCKAEDP HECYAKVEEE FKKLVEETQN LIKQNCELFE KLGE YYFQN ALLIRYTKKM PQVPTDTLVE LTRNLGKVGS KCCKLPEEKR LPCIEDYLSI VLNQLCVLHE KTPVNDRVTK CCTES LVNR RHCFSALEVD ETYVPKEFNA ETFTFHADLC TLSEEERQIM KQKFLVELVK HKPKATEEQL KAVMDDFTAF VEKCCK AED PETCFAEEGS KLIAKSQAAL GHHHHHH

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300
VitrificationCryogen name: ETHANE
DetailshSA3 was complexed with MgbAlb1 and the binary complex was isolated by Size exclusion chromatography. The density corresponding to NbAlb1 was not interpreted due to its low resolution.

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 316547
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING / Software - Name: cryoSPARC (ver. 4.4)
FSC plot (resolution estimation)

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