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- EMDB-53457: Combined map of singlet microtubule with 2 luminal protofilament -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-53457
TitleCombined map of singlet microtubule with 2 luminal protofilament
Map datacombined map (14pf-Microtubule 2 luminal protofilaments)
Sample
  • Complex: microtubule assembled by MAP6d1
    • Complex: tubulin
Keywordsmicrotubule / neuron / protein / CELL CYCLE
Biological speciesBos taurus (domestic cattle)
Methodsubtomogram averaging / cryo EM / Resolution: 12.5 Å
AuthorsGOPAL D / Wu J / Delaroche J / Bosc C / De Andrade M / Denarier E / Effantin G / Andrieux A / Gory-Faure S / Serre L / Arnal I
Funding support France, 1 items
OrganizationGrant numberCountry
Agence Nationale de la Recherche (ANR) France
CitationJournal: To Be Published
Title: The Mn-motif protein MAP6d1 assembles ciliary doublet microtubules
Authors: Gopal D / Wu J / Delaroche J / Bosc C / De Andrade M / Denarier E / Effantin G / Andrieux A / Gory-Faure S / Serre L / Arnal I
History
DepositionApr 18, 2025-
Header (metadata) releaseJul 9, 2025-
Map releaseJul 9, 2025-
UpdateJul 9, 2025-
Current statusJul 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53457.png

Downloads & links

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Map

FileDownload / File: emd_53457.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcombined map (14pf-Microtubule 2 luminal protofilaments)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.7 Å/pix.
x 192 pix.
= 518.4 Å		2.7 Å/pix.
x 192 pix.
= 518.4 Å		2.7 Å/pix.
x 192 pix.
= 518.4 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.7 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 2700.0
Minimum - Maximum1.0 - 4856.0
Average (Standard dev.)2422.058300000000145 (±396.722699999999975)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions192192192
Spacing192192192
CellA=B=C: 518.4 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : microtubule assembled by MAP6d1

EntireName: microtubule assembled by MAP6d1
Components
  • Complex: microtubule assembled by MAP6d1
    • Complex: tubulin

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Supramolecule #1: microtubule assembled by MAP6d1

SupramoleculeName: microtubule assembled by MAP6d1 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3
Source (natural)Organism: Bos taurus (domestic cattle)

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Supramolecule #2: tubulin

SupramoleculeName: tubulin / type: complex / ID: 2 / Parent: 1
Source (natural)Organism: Bos taurus (domestic cattle)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statefilament

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Sample preparation

BufferpH: 6.7
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 2.87 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 3.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 12.5 Å / Resolution method: FSC 0.5 CUT-OFF / Software - Name: EMAN2 / Number subtomograms used: 5881
ExtractionNumber tomograms: 252 / Number images used: 5881
CTF correctionSoftware - Name: EMAN2 / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Final angle assignmentType: NOT APPLICABLE

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