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Yorodumi- EMDB-53137: Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6... -
+ Open data
Open data
- Basic information
Basic information
| Entry |  | ||||||||||||||||||
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| Title | Cryo-EM structure of the PlPVC1 baseplate, 6-fold symmetrized (C6), in extended state | ||||||||||||||||||
|  Map data | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||||||||
|  Sample | 
 | ||||||||||||||||||
|  Keywords | Contractile injection system / VIRUS LIKE PARTICLE | ||||||||||||||||||
| Function / homology |  Function and homology information | ||||||||||||||||||
| Biological species |  Photorhabdus luminescens (bacteria) | ||||||||||||||||||
| Method | single particle reconstruction / cryo EM / Resolution: 2.68 Å | ||||||||||||||||||
|  Authors | Marin-Arraiza L / Taylor NMI | ||||||||||||||||||
| Funding support |  Denmark,  Spain, 5 items 
 | ||||||||||||||||||
|  Citation |  Journal: Nat Commun / Year: 2025 Title: Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states Authors: Marin-Arraiza L / Roa-Eguiara A / Pape T / Sofos N / Hendriks IA / Lund Nielsen M / Steiner-Rebrova EM / Taylor NMI | ||||||||||||||||||
| History | 
 | 
- Structure visualization
Structure visualization
| Supplemental images | 
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- Downloads & links
Downloads & links
-EMDB archive
| Map data |  emd_53137.map.gz | 661.7 MB |  EMDB map data format | |
|---|---|---|---|---|
| Header (meta data) |  emd-53137-v30.xml  emd-53137.xml | 28 KB 28 KB | Display Display |  EMDB header | 
| Images |  emd_53137.png | 46.3 KB | ||
| Filedesc metadata |  emd-53137.cif.gz | 8.5 KB | ||
| Others |  emd_53137_half_map_1.map.gz  emd_53137_half_map_2.map.gz | 1.2 GB 1.2 GB | ||
| Archive directory |  http://ftp.pdbj.org/pub/emdb/structures/EMD-53137  ftp://ftp.pdbj.org/pub/emdb/structures/EMD-53137 | HTTPS FTP | 
-Validation report
| Summary document |  emd_53137_validation.pdf.gz | 1 MB | Display |  EMDB validaton report | 
|---|---|---|---|---|
| Full document |  emd_53137_full_validation.pdf.gz | 1 MB | Display | |
| Data in XML |  emd_53137_validation.xml.gz | 23 KB | Display | |
| Data in CIF |  emd_53137_validation.cif.gz | 27.6 KB | Display | |
| Arichive directory |  https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53137  ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-53137 | HTTPS FTP | 
-Related structure data
| Related structure data |  9qglMC  9qgmC  9qgnC  9qgoC  9qgpC M: atomic model generated by this map C: citing same article ( | 
|---|---|
| Similar structure data | Similarity search - Function & homology  F&H Search | 
- Links
Links
| EMDB pages |  EMDB (EBI/PDBe) /  EMDataResource | 
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- Map
Map
| File |  Download / File: emd_53137.map.gz / Format: CCP4 / Size: 1.3 GB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||||||||||||||||||||||||||
| Projections & slices | Image control
 
 Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
| Voxel size | X=Y=Z: 1.2 Å | ||||||||||||||||||||||||||||||||||||
| Density | 
 | ||||||||||||||||||||||||||||||||||||
| Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
| Details | EMDB XML: 
 | 
-Supplemental data
-Half map: Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized,...
| File | emd_53137_half_map_1.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||
| Projections & Slices | 
 | ||||||||||||
| Density Histograms | 
-Half map: Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized,...
| File | emd_53137_half_map_2.map | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Annotation | Cryo-EM structure of the PlPVC baseplate, 6-fold symmetrized, in extended state | ||||||||||||
| Projections & Slices | 
 | ||||||||||||
| Density Histograms | 
- Sample components
Sample components
+Entire : PlPVC baseplate (C6) in extended state
+Supramolecule #1: PlPVC baseplate (C6) in extended state
+Macromolecule #1: Phage tail protein
+Macromolecule #2: Phage tail sheath family protein
+Macromolecule #3: Phage tail protein
+Macromolecule #4: Contractile injection system tube protein N-terminal domain-conta...
+Macromolecule #5: Photorhabdus luminescens subsp. laumondii TTO1 complete genome se...
+Macromolecule #6: Photorhabdus luminescens subsp. laumondii TTO1 complete genome se...
+Macromolecule #7: Baseplate protein J-like domain-containing protein
+Macromolecule #8: Phage tail sheath family protein
+Macromolecule #9: Phage tail sheath family protein
-Experimental details
-Structure determination
| Method | cryo EM | 
|---|---|
|  Processing | single particle reconstruction | 
| Aggregation state | particle | 
- Sample preparation
Sample preparation
| Buffer | pH: 7.4 | 
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| Vitrification | Cryogen name: ETHANE | 
- Electron microscopy
Electron microscopy
| Microscope | TFS KRIOS | 
|---|---|
| Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 41.0 e/Å2 | 
| Electron beam | Acceleration voltage: 300 kV / Electron source:  FIELD EMISSION GUN | 
| Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.6 µm | 
| Experimental equipment |  Model: Titan Krios / Image courtesy: FEI Company | 
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