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- EMDB-53051: Focused map of Complex I junction from Ovis aries in presence of ... -

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Basic information

Entry
Database: EMDB / ID: EMD-53051
TitleFocused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state
Map dataPost-processed map
Sample
  • Complex: Focused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state
KeywordsComplex I / inhibitor / respiration / proton pump / NADH:ubiquinone oxidoreductase / membrane protein / mitochondria / PROTON TRANSPORT
Biological speciesOvis aries (sheep)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.19 Å
AuthorsSazanov L / Petrova O
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Aging (NIH/NIA)2RF1AG055549-02A1 United States
CitationJournal: To Be Published
Title: Complex I from Ovis aries in presence of D1 inhibitor
Authors: Sazanov L / Petrova O
History
DepositionMar 7, 2025-
Header (metadata) releaseMar 18, 2026-
Map releaseMar 18, 2026-
UpdateMar 18, 2026-
Current statusMar 18, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_53051.png

Downloads & links

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Map

FileDownload / File: emd_53051.map.gz / Format: CCP4 / Size: 488.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationPost-processed map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.22 Å/pix.
x 504 pix.
= 614.88 Å		1.22 Å/pix.
x 504 pix.
= 614.88 Å		1.22 Å/pix.
x 504 pix.
= 614.88 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.22 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.14
Minimum - Maximum-0.21150869 - 0.7572007
Average (Standard dev.)0.00008254344 (±0.017339407)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions504504504
Spacing504504504
CellA=B=C: 614.88 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Focused refined map

Fileemd_53051_additional_1.map
AnnotationFocused refined map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_53051_half_map_1.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map

Fileemd_53051_half_map_2.map
AnnotationHalf map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Focused map of Complex I junction from Ovis aries in presence of ...

EntireName: Focused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state
Components
  • Complex: Focused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state

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Supramolecule #1: Focused map of Complex I junction from Ovis aries in presence of ...

SupramoleculeName: Focused map of Complex I junction from Ovis aries in presence of D1 inhibitor, Closed state
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Ovis aries (sheep)
Molecular weightTheoretical: 1 MDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration3 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R0.6/1 / Material: COPPER
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Number grids imaged: 1 / Number real images: 4167 / Average exposure time: 3.39 sec. / Average electron dose: 90.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 120000
Sample stageCooling holder cryogen: NITROGEN

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Image processing

Particle selectionNumber selected: 448410
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.19 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 50108
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
FSC plot (resolution estimation)

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