登録情報 データベース : EMDB / ID : EMD-52876 ダウンロードとリンクタイトル Open state without NUQM and with flavoprotein (classification state 3) of Pichia pastoris mitochondrial complex I in cMSP26 nanodiscs マップデータFinal consensus map used for model building and model refinement. Globally sharpened with RELION postprocess. 詳細 試料複合体 : Pichia Pastoris (Komagataella phaffi) mitochondrial complex I (NADH:ubiquinone oxidoreductase)リガンド : x 10種 詳細 キーワード NADH:ubiquinone oxidoreductase nanodisc membrane protein complex / MEMBRANE PROTEIN機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase complex / oxidoreductase activity, acting on NAD(P)H / NADH dehydrogenase activity / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly ... NADH:ubiquinone reductase (H+-translocating) / NADH dehydrogenase complex / oxidoreductase activity, acting on NAD(P)H / NADH dehydrogenase activity / acyl binding / ubiquinone binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / aerobic respiration / respiratory electron transport chain / electron transport chain / mitochondrial membrane / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / oxidoreductase activity / mitochondrial inner membrane / protein-containing complex binding / mitochondrion / metal ion binding / membrane 類似検索 - 分子機能 NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : ... NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / NADH dehydrogenase [ubiquinone] (complex I), 21kDa subunit, fungi / NADH-ubiquinone oxidoreductase, 21kDa subunit, C-terminal, fungi / NADH-ubiquinone oxidoreductase 9.5kDa subunit / C-terminal of NADH-ubiquinone oxidoreductase 21 kDa subunit / : / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 / NADH-ubiquinone oxidoreductase ASHI subunit (CI-ASHI or NDUFB8) / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / : / SLBB domain / : / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-quinone oxidoreductase, chain M/4 / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / NADH ubiquinone oxidoreductase, F subunit / NADH-quinone oxidoreductase, subunit D / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / Complex 1 LYR protein domain / NADH:ubiquinone oxidoreductase / Complex 1 protein (LYR family) / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / : / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / NADH:quinone oxidoreductase/Mrp antiporter, TM / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH:ubiquinone oxidoreductase, subunit 1, conserved site / Respiratory-chain NADH dehydrogenase subunit 1 signature 1. / Respiratory-chain NADH dehydrogenase subunit 1 signature 2. / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, subunit 1/F420H2 oxidoreductase subunit H / NADH dehydrogenase / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / 2Fe-2S iron-sulfur cluster binding domain / NAD-dependent epimerase/dehydratase / NuoE domain / NAD dependent epimerase/dehydratase family / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH-quinone oxidoreductase subunit E-like / NADH:ubiquinone oxidoreductase, 51kDa subunit, conserved site / Respiratory-chain NADH dehydrogenase 51 Kd subunit signature 2. / NADH-quinone oxidoreductase subunit E, N-terminal 類似検索 - ドメイン・相同性 Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Uncharacterized protein / NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) ... Subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Uncharacterized protein / NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NUCM (49 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUEM (39 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUFM (B13) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUGM (30 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUHM (24 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUIM (TYKY) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUJM (B14.7) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / BA75_00622T0 / NULM (ND4L) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUMM (13 kDa) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH-ubiquinone oxidoreductase / NUXM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / BA75_00589T0 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NIAM (ASHI) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NIDM (PDSW) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / BA75_05084T0 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NI8M (B8) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NI9M (B9) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NB2M (B12) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / BA75_04796T0 / NB5M (B15) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit / NESM (ESSS) subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / Acyl carrier protein / Acyl carrier protein / NUSM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUTM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) / NUUM subunit of mitochondrial NADH:ubiquinone oxidoreductase (Complex I) 類似検索 - 構成要素生物種 Komagataella pastoris (菌類)手法 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.34 Å 詳細 データ登録者Grba DN / Hirst J 資金援助 英国, 1件 詳細 詳細を隠すOrganization Grant number 国 Medical Research Council (MRC, United Kingdom) MC_UU_00015/2 英国
引用ジャーナル : Sci Adv / 年 : 2025タイトル : Global conformations of complex I are distinguished by the binding of a unique interdomain bridging subunit.著者 : Chris Seunggyu Lee / Daniel N Grba / John J Wright / Bozhidar S Ivanov / Judy Hirst / 要旨 : Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. ... Complex I (CI; NADH ubiquinone oxidoreductase) is central to energy generation and metabolic homeostasis in mammalian cells but contributes to adverse outcome pathways under challenging conditions. During ischemia, mammalian CI transitions from a turnover-ready, structurally "closed" state toward a dormant "open" state that prevents it from functioning in reverse during reperfusion to produce reactive oxygen species. Unfortunately, simpler, genetically tractable CI models do not recapitulate the same regulatory behavior, compromising mechanistic studies. Here, we report the structure of isolated CI from the yeast (-CI) and identify distinct closed and open states that resemble those of mammalian CI. Notably, a hitherto-unknown protein (NUQM) completes an interdomain bridge in only the closed state, implying that NUQM stabilizes it by restricting the conformational changes of opening. The direct correlation of NUQM binding with closed/open status in -CI provides opportunities for investigating regulatory mechanisms relevant to reversible catalysis and ischemia-reperfusion injury. 履歴 登録 2025年2月21日 - ヘッダ(付随情報) 公開 2025年10月15日 - マップ公開 2025年10月15日 - 更新 2025年10月15日 - 現状 2025年10月15日 処理サイト : PDBe / 状態 : 公開
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