Journal: Nat Struct Mol Biol / Year: 2025 Title: Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers. Authors: Tianyang Liu / Luyan Cao / Miroslav Mladenov / Guillaume Romet-Lemonne / Michael Way / Carolyn A Moores / Abstract: Branched actin networks nucleated by the Arp2/3 complex have critical roles in various cellular processes, from cell migration to intracellular transport. However, when activated by WISH/DIP/SPIN90- ...Branched actin networks nucleated by the Arp2/3 complex have critical roles in various cellular processes, from cell migration to intracellular transport. However, when activated by WISH/DIP/SPIN90-family proteins, Arp2/3 nucleates linear actin filaments. Here we found that human SPIN90 is a dimer that can nucleate bidirectional actin filaments. To understand the basis for this, we determined a 3-Å-resolution structure of human SPIN90-Arp2/3 complex nucleating actin filaments. Our structure shows that SPIN90 dimerizes through a three-helix bundle and interacts with two Arp2/3 complexes. Each SPIN90 molecule binds both Arp2/3 complexes to promote their activation. Our analysis demonstrates that single-filament nucleation by Arp2/3 is mechanistically more like branch formation than previously appreciated. The dimerization domain in SPIN90 orthologs is conserved in metazoans, suggesting that this mode of bidirectional nucleation is a common strategy to generate antiparallel actin filaments.
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Homo sapiens (human) / 
Sus scrofa (pig) / 
Amanita phalloides (death cap)
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emd_52580.png
http://ftp.pdbj.org/pub/emdb/structures/EMD-52580
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unidentified baculovirus
Escherichia coli (E. coli)
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Electron microscopy
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