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- EMDB-52566: Local Refinement of human M4 muscarinic acetylcholine receptor G ... -

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Basic information

Entry
Database: EMDB / ID: EMD-52566
TitleLocal Refinement of human M4 muscarinic acetylcholine receptor G protein complex bound to selective PAM Emraclidine
Map data
Sample
  • Complex: M4 mAChR bound to PAM Emraclidine in complex with dominant negative Galpha-il, Gbetal, Gamma2, and scFv16
KeywordsGPCR / MUSCARINIC / POSITIVE ALLOSTERIC MODULATOR / AGONIST / MEMBRANE PROTEIN / COMPLEX
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsTrabuco M / Sawicka M / Botte M / Vacca S
Funding support Switzerland, 1 items
OrganizationGrant numberCountry
Other private Switzerland
CitationJournal: To Be Published
Title: The Distinct Pharmacological Profile of Emraclidine, a Selective Positive Allosteric Modulator of the M4 Muscarinic Acetylcholine Receptor
Authors: Pin S / Wonsey-Quinn A / Trabuco M / Toh MF / Te L / Klein E / Catipay G / Smith DL / Gupta K / Hatch J / Nguyen H / Iredale P
History
DepositionJan 16, 2025-
Header (metadata) releaseJan 28, 2026-
Map releaseJan 28, 2026-
UpdateJan 28, 2026-
Current statusJan 28, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_52566.png

Downloads & links

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Map

FileDownload / File: emd_52566.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 360 pix.
= 298.8 Å		0.83 Å/pix.
x 360 pix.
= 298.8 Å		0.83 Å/pix.
x 360 pix.
= 298.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.83 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.29
Minimum - Maximum-1.2039858 - 1.8447794
Average (Standard dev.)-0.00029071796 (±0.018045586)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 298.8 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_52566_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_52566_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : M4 mAChR bound to PAM Emraclidine in complex with dominant negati...

EntireName: M4 mAChR bound to PAM Emraclidine in complex with dominant negative Galpha-il, Gbetal, Gamma2, and scFv16
Components
  • Complex: M4 mAChR bound to PAM Emraclidine in complex with dominant negative Galpha-il, Gbetal, Gamma2, and scFv16

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Supramolecule #1: M4 mAChR bound to PAM Emraclidine in complex with dominant negati...

SupramoleculeName: M4 mAChR bound to PAM Emraclidine in complex with dominant negative Galpha-il, Gbetal, Gamma2, and scFv16
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 157 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration8 mg/mL
BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

7trp

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 319415
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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