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- EMDB-52484: CRYO-EM FOCUSED REFINEMENT MAP OF HCT15 POLYSOMES IN HYBRID-PRE STATE -

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Open data


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Basic information

Entry
Database: EMDB / ID: EMD-52484
TitleCRYO-EM FOCUSED REFINEMENT MAP OF HCT15 POLYSOMES IN HYBRID-PRE STATE
Map dataBody 1 of focused refinement
Sample
  • Complex: HCT15 POLYSOMES IN HYBRID-PRE STATE
KeywordsCRYO-EM / HCT15 cancer / POLYSOMES / HYBRID-PRE STATE / RIBOSOME
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsRajan KS / Yonath A
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
European Research Council (ERC)European Union
CitationJournal: To Be Published
Title: CRYO-EM FOCUSED REFINEMENT MAP OF HCT15 POLYSOMES IN HYBRID-PRE STATE
Authors: Rajan KS / Yonath A
History
DepositionJan 8, 2025-
Header (metadata) releaseJan 21, 2026-
Map releaseJan 21, 2026-
UpdateJan 21, 2026-
Current statusJan 21, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_52484.png

Downloads & links

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Map

FileDownload / File: emd_52484.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationBody 1 of focused refinement
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.79 Å/pix.
x 480 pix.
= 380.64 Å		0.79 Å/pix.
x 480 pix.
= 380.64 Å		0.79 Å/pix.
x 480 pix.
= 380.64 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.793 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01
Minimum - Maximum-0.042971563 - 0.036657084
Average (Standard dev.)-0.00044092428 (±0.0025229969)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 380.63998 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Half map 2 for Body 3 of focused refinement

Fileemd_52484_additional_1.map
AnnotationHalf map 2 for Body 3 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Body 2 of focused refinement

Fileemd_52484_additional_2.map
AnnotationBody 2 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Body 3 of focused refinement

Fileemd_52484_additional_3.map
AnnotationBody 3 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map 1 for Body 2 of focused refinement

Fileemd_52484_additional_4.map
AnnotationHalf map 1 for Body 2 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map 1 for Body 3 of focused refinement

Fileemd_52484_additional_5.map
AnnotationHalf map 1 for Body 3 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Additional map: Half map 2 for Body 2 of focused refinement

Fileemd_52484_additional_6.map
AnnotationHalf map 2 for Body 2 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 1 for Body 1 of focused refinement

Fileemd_52484_half_map_1.map
AnnotationHalf map 1 for Body 1 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map 2 for Body 1 of focused refinement

Fileemd_52484_half_map_2.map
AnnotationHalf map 2 for Body 1 of focused refinement
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : HCT15 POLYSOMES IN HYBRID-PRE STATE

EntireName: HCT15 POLYSOMES IN HYBRID-PRE STATE
Components
  • Complex: HCT15 POLYSOMES IN HYBRID-PRE STATE

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Supramolecule #1: HCT15 POLYSOMES IN HYBRID-PRE STATE

SupramoleculeName: HCT15 POLYSOMES IN HYBRID-PRE STATE / type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 1.022 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.7 µm / Nominal defocus min: 0.6 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

6y57

Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 15395
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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