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データを開く
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基本情報
| 登録情報 | ![]() | |||||||||
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| タイトル | CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME WITH P/E-site tRNA AND mRNA : LM14Cs1H3 sKO STRAIN | |||||||||
マップデータ | ||||||||||
試料 |
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キーワード | CRYO-EM / LEISHMANIA MAJOR / 80S / RIBOSOME / LM14Cs1H3 / snoRNA | |||||||||
| 機能・相同性 | 機能・相同性情報ciliary transition zone / ciliary plasm / nuclear lumen / negative regulation of translational frameshifting / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / maturation of LSU-rRNA / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / rescue of stalled cytosolic ribosome ...ciliary transition zone / ciliary plasm / nuclear lumen / negative regulation of translational frameshifting / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / maturation of LSU-rRNA / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / rescue of stalled cytosolic ribosome / protein kinase C binding / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / maintenance of translational fidelity / modification-dependent protein catabolic process / protein tag activity / rRNA processing / kinase activity / ribosome biogenesis / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / protein ubiquitination / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleoplasm / nucleus / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
| 生物種 | Leishmania major strain Friedlin (大形リーシュマニア) | |||||||||
| 手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.5 Å | |||||||||
データ登録者 | Rajan KS / Yonath A | |||||||||
| 資金援助 | イスラエル, European Union, 2件
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引用 | ジャーナル: J Med Chem / 年: 2025タイトル: -Alkoxy-Substituted Anisomycins with Potent Anti-Trypanosomiasis Activity and Expanded Modes of Action. 著者: Kajumee Bora Bhowal / Anh Minh Thao Nguyen / Ana Victoria Ibarra-Meneses / Andressa Brito Lira / K Shanmugha Rajan / Jesus D Castaño / Francis Beaudry / Anat Bashan / Christopher Fernandez- ...著者: Kajumee Bora Bhowal / Anh Minh Thao Nguyen / Ana Victoria Ibarra-Meneses / Andressa Brito Lira / K Shanmugha Rajan / Jesus D Castaño / Francis Beaudry / Anat Bashan / Christopher Fernandez-Prada / Martin Olivier / Ada Yonath / William D Lubell / ![]() 要旨: Neglected tropical diseases caused by trypanosomatid parasites present a major public healthcare issue, partly due to emerging resistance. Attachment of ω-alkynyl chains characteristic of the lipid ...Neglected tropical diseases caused by trypanosomatid parasites present a major public healthcare issue, partly due to emerging resistance. Attachment of ω-alkynyl chains characteristic of the lipid tails of antiparasitic peptides to the -position of anisomycin gave ethers exhibiting potent activity, rivalling that of the parent ribosomal inhibitor, especially against resistant strains. Single-particle cryoelectron microscopy analysis revealed that -propargyl anisomycin binds to the highly conserved peptidyl transferase center of the ribosome similar to the parent inhibitor. Thermal proteomic profiling and gene ontology analysis demonstrated that -propargyl anisomycin exhibited a broader mode of action, including activity against glycosome-associated proteins. Alkynyl substituents improved antiparasitic activity against resistant strains, likely by enlarging the mode of action, offering a novel path toward therapy against trypanosomatid infections. | |||||||||
| 履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
| マップデータ | emd_52247.map.gz | 67.3 MB | EMDBマップデータ形式 | |
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| ヘッダ (付随情報) | emd-52247-v30.xml emd-52247.xml | 112.5 KB 112.5 KB | 表示 表示 | EMDBヘッダ |
| 画像 | emd_52247.png | 61.3 KB | ||
| Filedesc metadata | emd-52247.cif.gz | 22.2 KB | ||
| アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-52247 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-52247 | HTTPS FTP |
-検証レポート
| 文書・要旨 | emd_52247_validation.pdf.gz | 457 KB | 表示 | EMDB検証レポート |
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| 文書・詳細版 | emd_52247_full_validation.pdf.gz | 456.6 KB | 表示 | |
| XML形式データ | emd_52247_validation.xml.gz | 8.4 KB | 表示 | |
| CIF形式データ | emd_52247_validation.cif.gz | 9.8 KB | 表示 | |
| アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-52247 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-52247 | HTTPS FTP |
-関連構造データ
| 関連構造データ | ![]() 9hl9MC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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| 類似構造データ | 類似検索 - 機能・相同性 F&H 検索 |
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リンク
| EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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| 「今月の分子」の関連する項目 |
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マップ
| ファイル | ダウンロード / ファイル: emd_52247.map.gz / 形式: CCP4 / 大きさ: 1000 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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| 投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
| ボクセルのサイズ | X=Y=Z: 0.8245 Å | ||||||||||||||||||||||||||||||||||||
| 密度 |
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| 対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
| 詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : 80S RIBOSOME WITH P/E-site tRNA AND mRNA
+超分子 #1: 80S RIBOSOME WITH P/E-site tRNA AND mRNA
+分子 #1: LSUa_rRNA
+分子 #2: LSUb_rRNA
+分子 #3: SR1_rRNA
+分子 #4: SR2_rRNA
+分子 #5: SR4_rRNA
+分子 #6: SR6_rRNA
+分子 #7: 5.8S_rRNA
+分子 #8: 5S_rRNA
+分子 #51: P-site_tRNA
+分子 #52: E-site_tRNA
+分子 #53: mRNA
+分子 #88: SSU_rRNA
+分子 #9: Putative 60S ribosomal protein L2
+分子 #10: Putative ribosomal protein L3
+分子 #11: Putative ribosomal protein L1a
+分子 #12: 60S ribosomal protein L11
+分子 #13: Putative 60S ribosomal protein L9
+分子 #14: Putative 60S ribosomal protein L6
+分子 #15: 60S ribosomal protein L7a
+分子 #16: Putative 60S ribosomal protein L13a
+分子 #17: Putative 60S ribosomal protein L13
+分子 #18: Putative 60S ribosomal protein L23
+分子 #19: Putative 40S ribosomal protein L14
+分子 #20: Putative 60S ribosomal protein L27A/L29
+分子 #21: Ribosomal protein L15
+分子 #22: Putative 60S ribosomal protein L10
+分子 #23: Putative 60S ribosomal protein L5
+分子 #24: 60S ribosomal protein L18
+分子 #25: Putative 60S ribosomal protein L19
+分子 #26: 60S ribosomal protein L18a
+分子 #27: Putative 60S ribosomal protein L21
+分子 #28: Putative 60S ribosomal protein L17
+分子 #29: Putative 60S ribosomal protein L22
+分子 #30: Putative 60S ribosomal protein L23a
+分子 #31: Putative 60S ribosomal protein L26
+分子 #32: Putative ribosomal protein L24
+分子 #33: 60S ribosomal protein L27
+分子 #34: Putative 60S ribosomal protein L28
+分子 #35: Putative 60S ribosomal protein L35
+分子 #36: 60S ribosomal protein L29
+分子 #37: Putative 60S ribosomal protein L7
+分子 #38: 60S ribosomal protein L30
+分子 #39: Putative 60S ribosomal subunit protein L31
+分子 #40: 60S ribosomal protein L32
+分子 #41: Putative ribosomal protein l35a
+分子 #42: Putative 60S ribosomal protein L34
+分子 #43: Putative 60S Ribosomal protein L36
+分子 #44: Ribosomal protein L37
+分子 #45: Putative ribosomal protein L38
+分子 #46: Putative 60S ribosomal protein L39
+分子 #47: Ubiquitin-60S ribosomal protein L40
+分子 #48: Putative 60S ribosomal protein L41
+分子 #49: 60S ribosomal protein L37a
+分子 #50: Putative 60S ribosomal protein L44
+分子 #54: Small ribosomal subunit protein eS1
+分子 #55: 40S ribosomal protein SA
+分子 #56: Putative 40S ribosomal protein S3
+分子 #57: Putative 40S ribosomal protein S9
+分子 #58: 40S ribosomal protein S4
+分子 #59: 40S ribosomal protein S2
+分子 #60: 40S ribosomal protein S6
+分子 #61: 40S ribosomal protein S5
+分子 #62: 40S ribosomal protein S7
+分子 #63: Putative 40S ribosomal protein S15A
+分子 #64: 40S ribosomal protein S8
+分子 #65: Putative 40S ribosomal protein S16
+分子 #66: Putative ribosomal protein S20
+分子 #67: Putative 40S ribosomal protein S10
+分子 #68: 40S ribosomal protein S14
+分子 #69: Putative 40S ribosomal protein S23
+分子 #70: 40S ribosomal protein S12
+分子 #71: Putative 40S ribosomal protein S18
+分子 #72: Putative ribosomal protein S29
+分子 #73: Putative 40S ribosomal protein S13
+分子 #74: Ribosomal protein S17 family protein
+分子 #75: Putative 40S ribosomal protein S17
+分子 #76: Putative 40S ribosomal protein S15
+分子 #77: 40S ribosomal protein S19-like protein
+分子 #78: Putative 40S ribosomal protein S21
+分子 #79: 40S ribosomal protein S24
+分子 #80: 40S ribosomal protein S25
+分子 #81: 40S ribosomal protein S26
+分子 #82: Putative 40S ribosomal protein S27-1
+分子 #83: Putative 40S ribosomal protein S33
+分子 #84: 40S ribosomal protein S30
+分子 #85: Ubiquitin-60S ribosomal protein L40
+分子 #86: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #87: Putative RNA binding protein
+分子 #89: POTASSIUM ION
+分子 #90: SODIUM ION
+分子 #91: MAGNESIUM ION
+分子 #92: [(2R,3S,4S)-4-oxidanyl-2-[(4-prop-2-ynoxyphenyl)methyl]pyrrolidin...
+分子 #93: ZINC ION
+分子 #94: water
-実験情報
-構造解析
| 手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
| 試料の集合状態 | particle |
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試料調製
| 緩衝液 | pH: 7.6 |
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| 凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
| 顕微鏡 | TFS KRIOS |
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| 撮影 | フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) 平均電子線量: 1.0018 e/Å2 |
| 電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
| 電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
| 実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
ムービー
コントローラー
万見について




キーワード
Leishmania major strain Friedlin (大形リーシュマニア)
データ登録者
イスラエル, European Union, 2件
引用










Z (Sec.)
Y (Row.)
X (Col.)





















解析
FIELD EMISSION GUN

