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- EMDB-52192: Cryo-EM composite structure of 70S ribosome of marine cold bacter... -

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Basic information

Entry
Database: EMDB / ID: EMD-52192
TitleCryo-EM composite structure of 70S ribosome of marine cold bacterium Pseudoalteromonas translucida (P. haloplanktis)TAC125.
Map data
Sample
  • Complex: 70S Ribosome
    • Protein or peptide: x 48 types
    • RNA: x 4 types
  • RNA: x 1 types
  • Ligand: x 2 types
KeywordsRibosome / Cryo-EM / Composite structure / cold bacteria / translation
Function / homology
Function and homology information


assembly of large subunit precursor of preribosome / large ribosomal subunit / transferase activity / ribosomal small subunit assembly / ribosome binding / ribosome biogenesis / ribosomal small subunit biogenesis / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit ...assembly of large subunit precursor of preribosome / large ribosomal subunit / transferase activity / ribosomal small subunit assembly / ribosome binding / ribosome biogenesis / ribosomal small subunit biogenesis / 5S rRNA binding / ribosomal large subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / tRNA binding / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / cytosol / cytoplasm
Similarity search - Function
: / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily ...: / Ribosomal protein L25, long-form / Ribosomal protein L25, beta domain / Ribosomal protein L25, C-terminal / Ribosomal protein TL5, C-terminal domain / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein L9 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L9, C-terminal domain superfamily / : / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L28/L24 superfamily / Ribosomal protein L32p, bacterial type / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L9 / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S19, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S5, bacterial-type / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / : / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L20 signature. / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein L14P, bacterial-type / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein L33 / Ribosomal protein L2, bacterial/organellar-type / Ribosomal L28 family / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L28/L24 / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein L16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily
Similarity search - Domain/homology
Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 ...Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS20 / Large ribosomal subunit protein bL19 / Small ribosomal subunit protein bS16 / Large ribosomal subunit protein uL16 / Small ribosomal subunit protein uS3 / Large ribosomal subunit protein uL22 / Small ribosomal subunit protein uS19 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Small ribosomal subunit protein uS10 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein bL27 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein uL13 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein bL32 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein bS6 / Small ribosomal subunit protein bS21 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Small ribosomal subunit protein uS4 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL18 / Small ribosomal subunit protein uS5 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL15 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL25 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL35 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS12
Similarity search - Component
Biological speciesPseudoalteromonas translucida TAC125 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.36 Å
AuthorsSingh V / Godsora BKJ / Emmerich AG / Majumdar S / Sanyal S
Funding support Sweden, United States, 9 items
OrganizationGrant numberCountry
Swedish Research Council2023-05237 Sweden
Swedish Research Council2018-05498 Sweden
Swedish Research Council2016-06264 Sweden
Swedish Research Council2018-05498 Sweden
Swedish Research Council2018-05946 Sweden
Wenner-Gren FoundationUPD2023-0185 United States
Wenner-Gren FoundationUPD2017:0238 United States
Wenner-Gren FoundationUPD2018-0306 United States
Knut and Alice Wallenberg FoundationKAW 2017.0055 Sweden
CitationJournal: To Be Published
Title: Cryo-EM composite structure of 70S ribosome of marine cold bacterium Pseudoalteromonas translucida (P. haloplanktis)TAC125.
Authors: Singh V / Godsora BKJ / Emmerich AG / Majumdar S / Sanyal S
History
DepositionNov 26, 2024-
Header (metadata) releaseJul 15, 2026-
Map releaseJul 15, 2026-
UpdateJul 15, 2026-
Current statusJul 15, 2026Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_52192.png

Downloads & links

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Map

FileDownload / File: emd_52192.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.86 Å/pix.
x 512 pix.
= 440.32 Å		0.86 Å/pix.
x 512 pix.
= 440.32 Å		0.86 Å/pix.
x 512 pix.
= 440.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 4.5
Minimum - Maximum-7.7158256 - 33.362169999999999
Average (Standard dev.)-0.009551111 (±1.0184252)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 440.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : 70S Ribosome

EntireName: 70S Ribosome
Components
  • Complex: 70S Ribosome
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS14
    • RNA: 16S rRNA
    • RNA: 5S rRNA
    • Protein or peptide: Small ribosomal subunit protein uS2
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS13
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Small ribosomal subunit protein bS18
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Small ribosomal subunit protein bS21
    • Protein or peptide: Large ribosomal subunit protein bL33
    • Protein or peptide: Large ribosomal subunit protein bL34
    • Protein or peptide: Large ribosomal subunit protein bL35
    • Protein or peptide: Large ribosomal subunit protein bL36
    • Protein or peptide: Large ribosomal subunit protein uL2
    • Protein or peptide: Large ribosomal subunit protein uL3
    • Protein or peptide: Large ribosomal subunit protein uL4
    • Protein or peptide: Large ribosomal subunit protein uL6
    • Protein or peptide: Large ribosomal subunit protein bL9
    • Protein or peptide: Large ribosomal subunit protein uL13
    • Protein or peptide: Large ribosomal subunit protein uL14
    • Protein or peptide: Large ribosomal subunit protein uL15
    • Protein or peptide: Large ribosomal subunit protein uL16
    • Protein or peptide: Large ribosomal subunit protein bL17
    • Protein or peptide: Large ribosomal subunit protein uL18
    • Protein or peptide: Large ribosomal subunit protein bL19
    • Protein or peptide: Large ribosomal subunit protein bL20
    • Protein or peptide: Large ribosomal subunit protein bL21
    • Protein or peptide: Large ribosomal subunit protein uL22
    • Protein or peptide: Large ribosomal subunit protein uL23
    • Protein or peptide: Large ribosomal subunit protein uL24
    • Protein or peptide: Large ribosomal subunit protein bL25
    • Protein or peptide: Large ribosomal subunit protein bL27
    • Protein or peptide: Large ribosomal subunit protein bL28
    • Protein or peptide: Large ribosomal subunit protein uL29
    • Protein or peptide: Large ribosomal subunit protein uL30
    • Protein or peptide: Large ribosomal subunit protein bL32
    • Protein or peptide: Large ribosomal subunit protein uL5
    • RNA: P-site tRNA-fMet
    • RNA: mRNA
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Small ribosomal subunit protein uS7
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein uS9
  • RNA: 23S rRNA
  • Ligand: MAGNESIUM ION
  • Ligand: water

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Supramolecule #1: 70S Ribosome

SupramoleculeName: 70S Ribosome / type: complex / ID: 1 / Parent: 0
Macromolecule list: #3, #14, #1, #25, #2, #4, #6, #8, #11-#13, #15, #17-#18, #20-#21, #26-#53, #22-#23, #5, #16, #7, #10, #19, #9
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)

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Macromolecule #1: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 494.937156 KDa
SequenceString: AGAGUUUGAU CAUGGCUCAG AUUGAACGCU GGCGGCAGGC CUAACACAUG CAAGUCGAGC GGUAACAGAG AGGUGCUUGC ACCUUUGCU GACGAGCGGC GGACGGGUGA GUAAUGCUUG GGAACAUGCC UUGAGGUGGG GGACAACAGU UGGAAACGAC U GCUAAUAC ...String:
AGAGUUUGAU CAUGGCUCAG AUUGAACGCU GGCGGCAGGC CUAACACAUG CAAGUCGAGC GGUAACAGAG AGGUGCUUGC ACCUUUGCU GACGAGCGGC GGACGGGUGA GUAAUGCUUG GGAACAUGCC UUGAGGUGGG GGACAACAGU UGGAAACGAC U GCUAAUAC CGCAUAAUGU CUACGGACCA AAGGGGGCUU CGGCUCUCGC CUUUAGAUUG GCCCAAGUGG GAUUAGCUAG UU GGUGAGG UAAUGGCUCA CCAAGGCGAC GAUCCCUAGC UGGUUUGAGA GGAUGAUCAG CCACACUGGG ACUGAGACAC GGC CCAGAC UCCUACGGGA GGCAGCAGUG GGGAAUAUUG CACAAUGGGC GCAAGCCUGA UGCAGCCAUG CCGCGUGUGU GAAG AAGGC CUUCGGGUUG UAAAGCACUU UCAGUCAGGA GGAAAGGUUA GCAGUUAAUA CCUGCUAGCU GUGACGUUAC UGACA GAAG AAGCACCGGC UAACUCCGUG CCAGCAGCCG CGGUAAUACG GAGGGUGCGA GCGUUAAUCG GAAUUACUGG GCGUAA AGC GUACGCAGGC GGUUUGUUAA GCGAGAUGUG AAAGCCCCGG GCUCAACCUG GGAACUGCAU UUCGAACUGG CAAACUA GA GUGUGAUAGA GGGUGGUAGA AUUUCAGGUG UAGCGGUGAA AUGCGUAGAG AUCUGAAGGA AUACCGAUGG CGAAGGCA G CCACCUGGGU CAACACUGAC GCUCAUGUAC GAAAGCGUGG GGAGCAAACG GGAUUAGAUA CCCCGGUAGU CCACGCCGU AAACGAUGUC UACUAGAAGC UCGGAGCCUC GGUUCUGUUU UUCAAAGCUA ACGCAUUAAG UAGACCGCCU GGGGAGUACG GCCGCAAGG UUAAAACUCA AAUGAAUUGA CGGGGGCCCG CACAAGCGGU GGAGCAUGUG GUUUAAUUCG AU(2MG) (5MC)AACGC GAAGAACCUU ACCUACACUU GACAUACAGA GAACUUACCA GAGAUGGUUU GGUGCCUUCG GGAACUCUGA UACAGGUGC UGCAUGGCUG UCGUCAGCUC GUGUUGUGAG AUGUUGGGUU AAGUCCCGCA ACGAGCGCAA CCCCUAUCCU U AGUUGCUA GCAGGUAAUG CUGAGAACUC UAAGGAGACU GCCGGUGAUA AACCGGAGGA AGGUGGGGAC GACGUCAAGU CA UCAUGGC CCUUACGUGU AGGGCUACAC ACGUGCUACA AUGGCGCAUA CAGAGUGCUG CGAACUCGCG AGAGUAAGCG AAU CACUUA AAGUGCGUCG UAGUCCGGAU UGGAGUCUGC AACUCGACUC CAUGAAGUCG GAAUCGCUAG UAAUCGCGUA UCAG AAUGA CGCGGUGAAU ACGUUCCCGG GCCUUGUACA CACCG(4OC)CCGU CACACCAUGG GAGUGGGUUG CUCCAGAAGU A GAUAGUCU AACCCUCGGG AGGACGUUUA CCACGGAGUG AUUCAUGACU GGGGUGAAGU CG(UR3)AACAAGG UAGCCCUA G G(2MG)GA(MA6)CCUGG GGCUGGAUCA CCUCCUU

GENBANK: GENBANK: CR954246.1

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Macromolecule #22: P-site tRNA-fMet

MacromoleculeName: P-site tRNA-fMet / type: rna / ID: 22 / Number of copies: 1
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 5.771507 KDa
SequenceString:
GUAGGGCUCA UAACCCGA

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Macromolecule #23: mRNA

MacromoleculeName: mRNA / type: rna / ID: 23 / Number of copies: 1
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 1.264826 KDa
SequenceString:
AAUG

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Macromolecule #24: 23S rRNA

MacromoleculeName: 23S rRNA / type: rna / ID: 24 / Number of copies: 1
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 935.834438 KDa
SequenceString: GGUUAAGUGA CUAAGCGUAC ACGGUGGAUG CCUUGGCAGU UGGAGGCGAU GAAGGACGUA UUAACUUGCG AUAAGCCUAG UCAAGCUAG UAAAAAGCAC UUGAGACUAG GAUUUCCGAA UGGGGAAACC CACCUGCUUG CAGGUAUCGU UAACUGAAUA C AUAGGUUA ...String:
GGUUAAGUGA CUAAGCGUAC ACGGUGGAUG CCUUGGCAGU UGGAGGCGAU GAAGGACGUA UUAACUUGCG AUAAGCCUAG UCAAGCUAG UAAAAAGCAC UUGAGACUAG GAUUUCCGAA UGGGGAAACC CACCUGCUUG CAGGUAUCGU UAACUGAAUA C AUAGGUUA ACGAGGCGAA CGCGGAGAAC UGAAACAUCU AAGUACCCGU AGGAAAAGAA AUCAACCGAG AUUCCGAAAG UA GCGGCGA GCGAAAUCGG AACAGCCCUU AAGCUUAUUA UGUGUUAAUG GAAGGCUGCU GGAAAGCGCC ACGAUACAGG GUG AUAGUC CCGUACAUGA AAAGACAUUU UAAGUGAAAU CGAGUAGGUC GGAGCACGUG AAACUUUGAC UGAAUAUAGG UGGA CCAUC AUCUAAGGCU AAAUACUCCC AACUGACCGA UAGUGAACCA GUACCGUGAG GGAAAGGCGA AAAGAACCCC UGUGA GGGG AGUGAAAUAG AACCUGAAAC CGUGUACGUA CAAGCAGUAG GAGCCCACUU GUUGGGUGAC UGCGUACCUU UUGUAU AAU GGGUCAGCGA CUUAUAUUUU GUAGCGAGGU UAACCGAUUA GGGUAGCCGU AGGGAAACCG AGUCUUAACU GGGCGAA UA GUUGCAAGGU AUAGACCCGA AACCCGGUGA UCUAGCCAUG AGCAGGUUGA AGGUUGAGUA ACAUCAACUG GAGGACCG A ACCCACUAAC GUUGAAAAGU UAGGGGAUGA CUUGUGGUUA GGAGUGAAAG GCUAAUCAAA CCGGGAGAUA GCUGGUUCU CCCCGAAAUC UAUUUAGGUA GAGCCUCGGA CGAAUACUUA CGGGGGUAGA GCACUGUUAA GGCUAGGGGG UCAUCCCGAC UUACCAACC CUUUGCAAAC UCCGAAUACC GUAAAGUAAU AUCCGGGAGA CACACGGCGG GUGCUAACGU CCGUCGUGAA G AGGGAAAC AACCCAGACC GCCAGCUAAG GUCCCAAAGU CAUAGUUAAG UGGGAAACGA UGUGGAAAGG CCCAGACAGC CA GGAGGUU GGCUUAGAAG CAGCCAUCCU UUAAAGAAAG CGUAAUAGCU CACUGGUCGA GUCGGUCUGC GCGGAAGAUG UAA CGGGGC UAAACUAUGC ACCGAAGCUG CGGAUUCAUC UUAGGAUGAG UGGUAGGGGA GCGUUCUGUA AGCCGUUGAA GGUG UACCG GGAGGUAUGC UGGAGGUAUC AGAAGUGCGA AUGCUGACAU GAGUAACGAU AAUGGGAGUG AAAAACUCCC ACGCC GGAA GACCAAGGGU UCCUAUCCCA UGUUAAUCAG GGUAGGGUAA GUCGACCCCU AAGGCGAGGC CGAAAGGCGU AGUCGA UGG GAAACAGAUU AAUAUUUCUG UACUCGAUAU AAUUGCGAUG GGGGGACGGA GCAGGCUAAG CAAGCAUGGC GUUGGUA GU CCAUGUGAAA GUGAGUAGGG CGUUUGUUUA GGUAAAUCCG GACGAACACU AAACCUGAGA CACGAGACGA GUCACUAA G GUGAUGAAGU UGCUGAUGCC AUACUUCCAG GAAAAGCCUC UAAGCUUCAG AUUAUAUGGA AUCGUACCCC AAACCGACA CAGGUGGUCA GGUAGAGAAU ACUAAGGCGC UUGAGAGAAC UCGGGUGAAG GAACUAGGCA AAAUCGUACC GUAACUUCGG GAGAAGGUA CGCUCCUAUC UGUGAUGAGA CUUGCUCUCU AAGCGGACGG GAGCCGCAGU GACCAGGUGG CUGGGACUGU U UAUUAAAA ACACAGCACU GUGCAAAAUC GCAAGAUGAC GUAUACGGUG UGACACCU(2MG)C CCGGUGCCGG AAGGUUAA U UGAUGGGGUU AUCCUUAGGG AGAAGCUCUU GAUCGAAGCC CCGGUAAACG GCGGCCGUAA CUAUAACGGU CCUAAGGUA GCGAAA(5MU)UCC UUGUCGGGUA AGUUCCGACC UGCACGAAUG GUGUAACCAU GGCCACGCUG UCUCCACCCG AGACUC AGU GAAAUUGAAA UCGCAGUGAA GAUGCUGUGU ACCCGCGGCU AGACGGAAAG ACCCCGU(G7M)AA CCUUUACUAC AGC UUGGCA CUGAACAUUG AACCUACAUG UGUAGGAUAG GUGGGAGACU UUGAAGAUGA GACGCUAGUU UUGUUGGAGU CAAC CUUGA AAUACCACCC UUGUAGUUUU GAUGUUCUAA CGUUGGCCCC UGAAUCGGGG UUACGGACAG UGCCUGGUGG GUAGU UUGA CUG(OMG)GGCGGU CUCCUCCUAA AGAGUAACGG AGGAGCACGA AGGUUGGCUA AGUACGGUCG GACAUCGUAC GG UUAGUGU AAUGGUAGAA GCCAGCUUAA CUGCGAGACA GACACGUCGA GCAGGUACGA AAGUAGGUCA UAGUGAUCCG GUG GUUCUG AAUGGAAGGG CCAUCGCUCA ACGGAUAAAA GGUACUCCG(2MG) GGA(H2U)AACAGG CUGAUACCGC CCAAGA GUU CAUAUCGACG GCGGUGUUUG GCA(OMC)CUCG(2MA)U GUCGGCUCAU CACAUCCUGG GGCUGAAGUC GGUCCCAAG GGUAUGGC(OMU)G UUCGCCAUUU AAAGUGGUAC GCGAGCUGGG UUUAGAACGU CGUGAGACAG UUCGGUCCCU AUCUGC CGU GGGCGUUUGA GAAUUGAGAG GGGUUGCUCC UAGUACGAGA GGACCGGAGU GAACGAACCG CUGGUGUUCG GGUUGUC AU GCCAAUGGCA UUGCCCGGUA GCUACGUUCG GAACUGAUAA GCGCUGAAAG CAUCUAAGCG CGAAGCAGGC CUCGAGAU G AUUUCUCACU AGAGCUAUAA GCUCUCUGAA GGGCCGUUGG AGACUACAAC GUUGAUAGGC AAGAUGUGGA AGUGCUGUG AGGCAUUAAG CUAACUUGUA CUAAUUACCC GUGAGGCUUA ACCAU

GENBANK: GENBANK: CR954246.1

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Macromolecule #25: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 25 / Number of copies: 1
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 37.68141 KDa
SequenceString:
UGCUUGGUAA CAAUAGCGUU UUGGACCCAC CUGACCCCAU GCCGAACUCA GUAGUGAAAC GAAACAGCGC CGAUGAUAGU GUAGCAUUU GCUAUGUGAA AGUAGGACAU UGCCAGGC

GENBANK: GENBANK: NR_103283.1

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Macromolecule #2: Small ribosomal subunit protein uS2

MacromoleculeName: Small ribosomal subunit protein uS2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 26.878674 KDa
SequenceString: MANVSMRDML QAGVHFGHQA RYWNPKMKPF IFGTRNRVHI INLEQTVPMF NTALKFLSGA AANKGKVLFV GTKRAASEAV KESAIASDQ YYVNHRWLGG MLTNWKTVRQ SIKRLKDLEI QSQDGTFEKL TKKETLMLNR EMEKLEKSLG GIKNMGGLPD A LFVIDADH ...String:
MANVSMRDML QAGVHFGHQA RYWNPKMKPF IFGTRNRVHI INLEQTVPMF NTALKFLSGA AANKGKVLFV GTKRAASEAV KESAIASDQ YYVNHRWLGG MLTNWKTVRQ SIKRLKDLEI QSQDGTFEKL TKKETLMLNR EMEKLEKSLG GIKNMGGLPD A LFVIDADH EHIAIREANN LGIPVVAIVD TNSNPDGVDF IVPGNDDAIR AVSLYTNAVA TAITEGRENT LVAQAEKDDF VE AE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 25.78018 KDa
SequenceString: MGQKVHPTGI RLGISKPWVS TWYASSKDFS AQLFGDHKVR TFLTKELKTA SVSKIVIERP AKSIRVTIHT ARPGVVIGKK GEDVEKLRQ AVTKIAGVPA QINISEVRKP ELDAQLVADG IASQLERRVM FRRAMKRSVQ NAMRIGSKGI KVEVSGRLGG A EIARTEWY ...String:
MGQKVHPTGI RLGISKPWVS TWYASSKDFS AQLFGDHKVR TFLTKELKTA SVSKIVIERP AKSIRVTIHT ARPGVVIGKK GEDVEKLRQ AVTKIAGVPA QINISEVRKP ELDAQLVADG IASQLERRVM FRRAMKRSVQ NAMRIGSKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYATSE ALTTYGIIGV KVWIFKGEVI GGLPLVQEQE KPAKRAPKKA KKSAK

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #4: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 23.525191 KDa
SequenceString: MARYLGPKLK LSRREGTDLF LKSGVRAIDS KCKLETAPGQ HGARKGRLSD YGLQLREKQK VRRIYGVLEK QFRNYYKEAA RLKGNTGEN LLQLLEQRLD NVVYRMGFAS TRAEARQLVS HKAILVNGRV VNVPSYVIAP EDTVVIREKS KTQARIIAAL E LAEQREKP ...String:
MARYLGPKLK LSRREGTDLF LKSGVRAIDS KCKLETAPGQ HGARKGRLSD YGLQLREKQK VRRIYGVLEK QFRNYYKEAA RLKGNTGEN LLQLLEQRLD NVVYRMGFAS TRAEARQLVS HKAILVNGRV VNVPSYVIAP EDTVVIREKS KTQARIIAAL E LAEQREKP TWVEVDGKKL EGSFKRLPER SDLSADINEQ LIVELYSK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #5: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 17.735525 KDa
SequenceString:
MANVEAKQQQ PDLAEKLIAV NRVSKVVKGG RIFSFTALTV VGDGAGKVGF GYGKAREVPA AIQKAMEKAR RNMINVDLNG NTLQHPVKG RHAGSQVFMK PASEGTGIIA GGAMRAVLEV TGVQNVLSKC YGSTNPINVV RATISALENM NSPQSIAAKR G LRIDEILG

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #6: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.013841 KDa
SequenceString:
MRHYEIVFMV HPDQSEQVTG MIERYTGSIT EAGGVIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPTEV ISELETTFRY NDAVLRNLV MRTKNAVTEA SPLVREEKKE APAA

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #7: Small ribosomal subunit protein uS7

MacromoleculeName: Small ribosomal subunit protein uS7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 17.627541 KDa
SequenceString:
MPRRRVIGQR KILPDPKFGS ELLAKFVNIV MLDGKKSTAE KIVYGALNVA AEKSGKSHLE IFETALDNIR PQVEVKSRRV GGSTYQVPV EVRPVRRNAL GMRWLVDAAR KRGEKSMGLR LAQEIVDAAD NKGTAVKKRE DVHRMAEANK AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #8: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 14.082479 KDa
SequenceString:
MSLQDPIADL FTRIRNGQSA KKVSVTMPNS KLKVAVAKVL KNEGYIADFA INGDVKPELS IELKYFEGKA VIENIQRVSR PGLRIYKRR DELPKVMGGL GIAIVSTSKG LMTDRAARSA GVGGEIIGFV A

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #9: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 14.638953 KDa
SequenceString:
MANQYYGTGR RKSSSARVFL RPGTGNIVIN KRSLEEYFGR ETARMVVHQA LELVDMTEKF DLHITVSGGG TTGQAGAIRH GITRALMEF DESLRPQLRK AGFVTRDARK VERKKVGLKK ARKRPQFSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #10: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.752508 KDa
SequenceString:
MSNQRIRIRL KAFDHRLIDQ STAEIVETAK RTGAQVRGPI PLPTRFERFT VLTSPHVNKD ARDQYEIRTH KRLIDIVEPT DKTVDALMR LDLAAGVDVQ ISLG

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #11: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.71873 KDa
SequenceString:
MAKTPIRRKK VKKQVADGMA HVHASFNNTI VTITDRQGNA LSWATAGGSG FRGSRKSTPF AAQVAAERAG TAAQEYGLKN LEVFIKGPG PGRESAVRAL NAAGYRITNI TDVTPIPHNG CRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #12: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.699979 KDa
SequenceString:
MATINQLVRK PRRSKVTKSN SAALKACPQK RGVCTRVYTT TPKKPNSALR KVARVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVKD LPGVRFHTVR GALDCAGVSD RRQARSKYGT KRPKG

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #13: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.142433 KDa
SequenceString:
MARIAGINVP DHKHAVIGLT AIYGIGKTRS KAILAATGIA ETTKIGELSD ETLDVLRDAV GKYTVEGDLR REVTLNIKRL MDLGCYRGL RHRRSLPLRG QRTKTNARTR KGPRKPIKR

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #14: Small ribosomal subunit protein uS14

MacromoleculeName: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.518493 KDa
SequenceString:
MAKNSMKARE AKRTKLVAQF AEKRTALKAI ISDVNTSEDD RWDAVLKLQA LPRDSSPVRQ RNRCNITGRP HGFLRKFGMS RIKVREAAM RGEIPGLKKA SW

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #15: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 10.296922 KDa
SequenceString:
MSLSNQEKID IIAKFARAEG DTGSPEVQVA LLTFDINKLQ GHFADHKHDF HSRRGLLRKV SQRRKLLDYL KGKDITRYTA LIKELGLRR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #16: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 9.271763 KDa
SequenceString:
MVTIRLQRGG AKKRPFYQIV VADSRFSRDG RFIEKVGFFN PIAAGQEEKI RLDLPRVEHW VGQGASLSDR VAKLVKDARK AA

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #17: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 9.789394 KDa
SequenceString:
MSDKIRTLQG RVISDKMEKS FTIAIARYVK HPIYGKFIRR TTKLHVHDEN NEVQAGDVVT IRECAPISKS KSWTFVAVIE RPKQA

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #18: Small ribosomal subunit protein bS18

MacromoleculeName: Small ribosomal subunit protein bS18 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 8.86136 KDa
SequenceString:
MARYFRRRKF CRFKAEGVQQ IDYKDLATLR NYVTESGKIV PSRITGTSAK YQRQLATAIK RARYLALLPY TDLHK

UniProtKB: Small ribosomal subunit protein bS18

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Macromolecule #19: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 10.969804 KDa
SequenceString:
MRNSHATFSQ EGSFYRPTLL KKVEKALESG DKKPIKTWSR RSMIIPNMIG LTIAVHNGRQ HVPVFITDEM IGHKLGEFAP TRTYRGHAA DKKAKKR

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #20: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 9.659411 KDa
SequenceString:
MANIKSAKKR AITSEKNRQH NASRRSMMRT YFKKVIVAIE AGDKEAAQQA FSVATPILDR YATKGLIHKN KAARHKSRIA AKIKAL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #21: Small ribosomal subunit protein bS21

MacromoleculeName: Small ribosomal subunit protein bS21 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 8.589221 KDa
SequenceString:
MPVIKVRENE PFDVALRRFK RSCEKAGILS EVRRREHYEK PTAERKRKKA AAVKRHMKKL SRDNARRVKL Y

UniProtKB: Small ribosomal subunit protein bS21

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Macromolecule #26: Large ribosomal subunit protein bL33

MacromoleculeName: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 6.13137 KDa
SequenceString:
MRDKIRLVST AGTGFFYTTD KNKRNMPEKM EIKKFDPKIR KHVLFKEAKI K

UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #27: Large ribosomal subunit protein bL34

MacromoleculeName: Large ribosomal subunit protein bL34 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 5.109244 KDa
SequenceString:
MKRTFQPSVL KRKRAHGFRA RMATVNGRKV LARRRAKGRK VLSA

UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #28: Large ribosomal subunit protein bL35

MacromoleculeName: Large ribosomal subunit protein bL35 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 7.717437 KDa
SequenceString:
MAYKLKSHRG AAKRFKKTAS GGFKRKQAHL RHILTKKTSK RKLHLRPKMM VHKNDHGLVS RMLPFA

UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #29: Large ribosomal subunit protein bL36

MacromoleculeName: Large ribosomal subunit protein bL36 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 5.00621 KDa
SequenceString:
MKVLSSLKSA KQRAGCQVVK RKGRVFVICK ENPRFKAVQG MKKK

UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #30: Large ribosomal subunit protein uL2

MacromoleculeName: Large ribosomal subunit protein uL2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 29.956568 KDa
SequenceString: MALQKCKPTS AGRRHLVKVV NSDLHKGKPY APLLEKNSKS GGRNNNGRIT VRHIGGGHKQ HYRLIDFKRT KDGIPATVER LEYDPNRSA NIALVLYADG ERRYIIAPKG LKAGDAIQSG VDAPIKPGNA LPMRNMPVGS TVHNVELKPG KGAQIARSAG A YVQILARD ...String:
MALQKCKPTS AGRRHLVKVV NSDLHKGKPY APLLEKNSKS GGRNNNGRIT VRHIGGGHKQ HYRLIDFKRT KDGIPATVER LEYDPNRSA NIALVLYADG ERRYIIAPKG LKAGDAIQSG VDAPIKPGNA LPMRNMPVGS TVHNVELKPG KGAQIARSAG A YVQILARD GQYVTLRLRS GEVRKVEADC RATLGEVGNA EHMLRSLGKA GANRWRGIRP TVRGVAMNPV DHPHGGGEGR TS GGRHPVS PWGKPTKGAK TRKNKRTDKF IVRRRTK

UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #31: Large ribosomal subunit protein uL3

MacromoleculeName: Large ribosomal subunit protein uL3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 22.214338 KDa
SequenceString: MALGLVGRKV GMTRIFTEDG VSIPVTVIEA TPNRIAQIKS EATDGYNALQ VTAGTKKASR VNKASAGHFA KAGVEAGRGL WEFRLNGGE GDFEVGAELT VELFNEINKV DVTGTSKGKG FQGGVKRWNF SMQDATHGNS LSHRAPGSIG QNQSPGKVFK G KKMAGHMG ...String:
MALGLVGRKV GMTRIFTEDG VSIPVTVIEA TPNRIAQIKS EATDGYNALQ VTAGTKKASR VNKASAGHFA KAGVEAGRGL WEFRLNGGE GDFEVGAELT VELFNEINKV DVTGTSKGKG FQGGVKRWNF SMQDATHGNS LSHRAPGSIG QNQSPGKVFK G KKMAGHMG AERVTTQNLE LVRVDAERNL LLVKGAVPGA IGGDVIVKPA VKA

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #32: Large ribosomal subunit protein uL4

MacromoleculeName: Large ribosomal subunit protein uL4 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 21.86215 KDa
SequenceString: MELAIKDASG GLEVSEATFG REFNEALVHQ VVVAYAAGAR QGTRAQKTRS EVSGGGKKPW AQKGTGRARA GTIRSPIWRS GGVSFAAKP QDHSQKVNRK MYRGAIKSIL SELVRQERLI VVESFALAAP KTKELVAKLK ELELKDVLIV TEEVDENLFL S ARNLYKVD ...String:
MELAIKDASG GLEVSEATFG REFNEALVHQ VVVAYAAGAR QGTRAQKTRS EVSGGGKKPW AQKGTGRARA GTIRSPIWRS GGVSFAAKP QDHSQKVNRK MYRGAIKSIL SELVRQERLI VVESFALAAP KTKELVAKLK ELELKDVLIV TEEVDENLFL S ARNLYKVD TRDVAGIDPV SLIAFDKVLI TAAAVKQLEE ALA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #33: Large ribosomal subunit protein uL6

MacromoleculeName: Large ribosomal subunit protein uL6 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 19.233113 KDa
SequenceString:
MSRIAKAPIS VPAGVEVTLK GQEITVKGKN GELTRTINNA VEVKVEENVI TTLPREGVTD AWAQAGTARA LINNMIVGTH EGYEKKLQL VGVGYRAAAK GKTLDLTLGF SHPVHFAVPE GITIETPSQT EVLVKGVDKQ LVGQTAANIR AYRKPEPYKG K GVRYSDEN VRRKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #34: Large ribosomal subunit protein bL9

MacromoleculeName: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 15.745044 KDa
SequenceString:
MQVILLDKIA NLGGLGDQVV VKSGFARNFL FPQGKAVPAT KANIETFDAR RAELEAKIAD QLVAAQARAD KLEALAEVTL VSKAGDEGK LFGSIGTRDI ADAISAVGVE VAKSEVRLPL GTIRETGEFD VSIAVHSEVT ATIKVIVIAE A

UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #35: Large ribosomal subunit protein uL13

MacromoleculeName: Large ribosomal subunit protein uL13 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 15.972583 KDa
SequenceString:
MKTFVAKPET VKRDWYVVDA EGKTLGRIAT EIALRLRGKH KVEYTPHVDT GDYIIVINAE KVTVTGNKFK NKVYYSHSGF PGGLKSTTF DKLQAAKPEM IIEKAVKGML PRGPLGRAMY RKLKVYTGTE HNHAAQQPQV LDI

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #36: Large ribosomal subunit protein uL14

MacromoleculeName: Large ribosomal subunit protein uL14 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.443841 KDa
SequenceString:
MIQMQTQLEV ADNSGARKVQ CIKVLGGSHR RYAAIGDIIK VSVKEAIPRG KVKKGDVKNA VVVRTKKGVR RPDGSLIRFD SNAAVILND NLQPIGTRIF GPVTRELRND KFMKIVSLAP EVL

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #37: Large ribosomal subunit protein uL15

MacromoleculeName: Large ribosomal subunit protein uL15 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 15.012455 KDa
SequenceString:
MHLNTLSPAA GSKTAPKRVG RGIGSGLGKT GGRGHKGQKS RSGGKVRVGF EGGQMPMQRR LPKFGFTSRK SLVSAEINLY EIAKVEGDV VDLSALQAAG LVKKNVLFVK VVKSGEVSRA VTVKGLRATK GAREAIEAAG GKVED

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #38: Large ribosomal subunit protein uL16

MacromoleculeName: Large ribosomal subunit protein uL16 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 15.694476 KDa
SequenceString:
MLQPKRTKFR KTHKGRNRGL AQNGNKVSFG TFGLKATGRG RMTARQIEAA RRAMTRHVKR QGKIWIRVFP DKPITNKPLE VRMGKGKGS VEYWVAEIQP GKVLYEMEGV SEELAREAFD LAARKLPFKT TFVTRTVM

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #39: Large ribosomal subunit protein bL17

MacromoleculeName: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 14.875089 KDa
SequenceString:
MRHRKSGRQL NRNSSHRKAM FSNMAGSLVK HEIIKTTLPK AKELRRVIEP LITLAKTDSV ANRRLAFART RDNEVVGKLF SEIGPRNAD RPGGYTRILK CGFRTGDNAP MAYIELVGRP ATSEAVQEDA QSAE

UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #40: Large ribosomal subunit protein uL18

MacromoleculeName: Large ribosomal subunit protein uL18 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 12.633553 KDa
SequenceString:
MDKKTARLRR AKRTRRNYIE QGTTRLVIHR TPRHIYAQVV TAEGTVLAAA STVEKAICET VKGTGNVAAA QAVGKAVAER AADKGIEKI AFDRSGFKYH GRVKALADAA REAGLQF

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #41: Large ribosomal subunit protein bL19

MacromoleculeName: Large ribosomal subunit protein bL19 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.356467 KDa
SequenceString:
MAKVNQNIIK ALEDEQLKTD VPAFGAGDTV VVQVKVKEGA KERLQAFEGV VIAKRNRGLH SAFTVRKISN GEGVERVFQT HSPLIDSVT VKRRGAVRRA KLYYLRERSG KSARIREKLN

UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #42: Large ribosomal subunit protein bL20

MacromoleculeName: Large ribosomal subunit protein bL20 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 13.593929 KDa
SequenceString:
MARVKRGVVA RARHKKVLKQ AKGYYGARSR VYRVAFQAVT KAGQYAYRDR RAKKRTFRQL WIARINAASR QNGLSYSRFI SGLKKTSVE IDRKILADIA VYDQVAFAAL VEKAKEGLAA A

UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #43: Large ribosomal subunit protein bL21

MacromoleculeName: Large ribosomal subunit protein bL21 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.513281 KDa
SequenceString:
MYAVFQSGGK QHRVTEGQTI RLEKLDVETG AAVEFDSVLL VADGEKIEIG VPFVNGGKIT AEVVSHGRGE KIKVVKFRRR KHSRKQMGH RQWFTEVKIT GISA

UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #44: Large ribosomal subunit protein uL22

MacromoleculeName: Large ribosomal subunit protein uL22 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 12.056074 KDa
SequenceString:
MQALAKHKFA SGSAQKARLV ADQIRGLPVD RALEILAYSP KKAAVLVKKV LESAIANAEH NEGADIDELR ITTIFVDDGP TMKRIMPRA KGRADRILKR TSHITVMVSD S

UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #45: Large ribosomal subunit protein uL23

MacromoleculeName: Large ribosomal subunit protein uL23 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.154875 KDa
SequenceString:
MIREERLLKV IVAPHISEKS TIAAEENNTI VFKVAKDSTK AEVKAAVEKL FEVEVTGVRT LNVKGKTKRT GARFGRRNDW KKAYVTLKE GSELDFVGGA E

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #46: Large ribosomal subunit protein uL24

MacromoleculeName: Large ribosomal subunit protein uL24 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.411287 KDa
SequenceString:
MAAKIRRDDE VIVLAGKDKG KRGKVLSVVT ESGRLFVEGI NLIKKHQKPV PQLNQPGGIV EKEASIDVSN VAIYNSETSK ADRVGFKIE DGKKLRIFKS TGKTI

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #47: Large ribosomal subunit protein bL25

MacromoleculeName: Large ribosomal subunit protein bL25 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 22.37227 KDa
SequenceString: MSNKTYVYNA ELREQTGTGA SRRLRREDKV PAIVYGANKE ATSITLDHKD VIKAQESEGF YTQILTLNIG GESVETILKD IQRHPFKPK VTHLDFQRID ANQKLHTIVP IHFVGEDVVE KLGTTVVRQL TEIEISCFPK AIPDFVTVDV SKLVAGDSLH I SDVALPAG ...String:
MSNKTYVYNA ELREQTGTGA SRRLRREDKV PAIVYGANKE ATSITLDHKD VIKAQESEGF YTQILTLNIG GESVETILKD IQRHPFKPK VTHLDFQRID ANQKLHTIVP IHFVGEDVVE KLGTTVVRQL TEIEISCFPK AIPDFVTVDV SKLVAGDSLH I SDVALPAG VSSIDLAKGP GHDQSILIVK ANKSAPTEDE AEDAAE

UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #48: Large ribosomal subunit protein bL27

MacromoleculeName: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 9.270611 KDa
SequenceString:
MAHKKAAGST RNGRDSESKR LGVKRFGGES VLAGSIIVRQ RGTRFHPGTN VGIGKDHTIF AKADGKVQFE QKGPLNRKYV TIVTE

UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #49: Large ribosomal subunit protein bL28

MacromoleculeName: Large ribosomal subunit protein bL28 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 11.106158 KDa
SequenceString:
MALGKIKLEL SYWRYIDMAK VCQVTGKRPA VGNHRSHARN ATKRRFLPNL QTHRFWVESE KRFVTLRTTT KGMRIIDKKG IDTVLTEIR ARGVKV

UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #50: Large ribosomal subunit protein uL29

MacromoleculeName: Large ribosomal subunit protein uL29 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 7.195311 KDa
SequenceString:
MKASELKDKS VEELNTELLG LLREQFNMRM QASTGQLAQT HTLRTVRRDI ARVKTVINQK AGQ

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #51: Large ribosomal subunit protein uL30

MacromoleculeName: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 6.675873 KDa
SequenceString:
MANQTVKVTQ VKSSIGRLPK HKATLRGLGL RRINHTVELE DTACVRGMIN QVSYMVKVEG

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #52: Large ribosomal subunit protein bL32

MacromoleculeName: Large ribosomal subunit protein bL32 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 6.280132 KDa
SequenceString:
MAVQKSKKSR ARRGMRRSHD AISGPSLTVD QTSGETHRRH HVTADGYYKG VQVISK

UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #53: Large ribosomal subunit protein uL5

MacromoleculeName: Large ribosomal subunit protein uL5 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pseudoalteromonas translucida TAC125 (bacteria)
Molecular weightTheoretical: 20.126396 KDa
SequenceString:
MAKLHEVYKD KVVAELQEKF GFSSVMQVPL IEKITLNMGV GEALADKKIL DNAVADLAAI SGQKPLITKA RKSVAGFKVR EGYPIGCKV TLRGERMWDF FERLVSIAIP RIRDFRGVSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGMDITITTS A KSDEEGRA LLEAFNFPFK K

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #54: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 54 / Number of copies: 289 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #55: water

MacromoleculeName: water / type: ligand / ID: 55 / Number of copies: 27 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
GridModel: Quantifoil / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE
DetailsNative ribosomes purified from log phase culture of Pseudoalteromonas haloplanktis TAC125 using conventional ultracentrifugation method.

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 1.25 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 5.0 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: cryoSPARC (ver. ver. v3.3.1+220118) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.36 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. ver. v3.3.1+220118) / Number images used: 360324
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. ver. v3.3.1+220118)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. ver. v3.3.1+220118)

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