ジャーナル: Nat Commun / 年: 2025 タイトル: Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets. 著者: Aditya Chhatre / Ludek Stepanek / Adrian Pascal Nievergelt / Gonzalo Alvarez Viar / Stefan Diez / Gaia Pigino / 要旨: Cilia assembly and function rely on the bidirectional transport of components between the cell body and ciliary tip via Intraflagellar Transport (IFT) trains. Anterograde and retrograde IFT trains ...Cilia assembly and function rely on the bidirectional transport of components between the cell body and ciliary tip via Intraflagellar Transport (IFT) trains. Anterograde and retrograde IFT trains travel along the B- and A-tubules of microtubule doublets, respectively, ensuring smooth traffic flow. However, the mechanism underlying this segregation remains unclear. Here, we test whether tubulin detyrosination (enriched on B-tubules) and tyrosination (enriched on A-tubules) have a role in IFT logistics. We report that knockout of tubulin detyrosinase VashL in Chlamydomonas reinhardtii causes frequent IFT train stoppages and impaired ciliary growth. By reconstituting IFT train motility on de-membranated axonemes and synthetic microtubules, we show that anterograde and retrograde trains preferentially associate with detyrosinated and tyrosinated microtubules, respectively. We propose that tubulin tyrosination/detyrosination is crucial for spatial segregation and collision-free IFT train motion, highlighting the significance of the tubulin code in ciliary transport.
想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 22.56 Å / 解像度の算出法: OTHER 詳細: We hit Nyquist at 11.28A pixel size (binned tomograms) 使用したサブトモグラム数: 841
抽出
トモグラム数: 6 / 使用した粒子像数: 841
最終 角度割当
タイプ: OTHER / 詳細: Cross correlation coefficient (PEET software)