[English] 日本語
Yorodumi
- EMDB-51914: Hexagonally Ordered Nanofibrils Easily Yield by dual Amphiphilic ... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: EMDB / ID: EMD-51914
TitleHexagonally Ordered Nanofibrils Easily Yield by dual Amphiphilic Self-Assembling Peptide
Map dataMap 3A around the atomic model
Sample
  • Complex: Nanofibrils from dual amphiphilic synthetic peptide
    • Protein or peptide: Peptide LYS-VAL-LYS-VAL-SER-GLN-ILE-ASN-MET
Keywordssynthetic peptide / peptide nanofibril / amyloid / HONEY ASAP / PROTEIN FIBRIL
Biological speciesother sequences (unknown)
Methodhelical reconstruction / cryo EM / Resolution: 2.96 Å
AuthorsMazzotta F / Baptista LA / Schmidt M / Gacanin J / Lieberwirth I / Weil T / Landfester K
Funding supportEuropean Union, 1 items
OrganizationGrant numberCountry
H2020 Marie Curie Actions of the European Commission860914European Union
CitationJournal: To Be Published
Title: Hexagonally Ordered Nanofibrils Easily Yield by dual Amphiphilic Self-Assembling Peptide: HONEY ASAP
Authors: Mazzotta F / Baptista LA / Schmidt M / Lieberwirth I / Landfester K
History
DepositionOct 28, 2024-
Header (metadata) releaseMay 6, 2026-
Map releaseMay 6, 2026-
UpdateMay 6, 2026-
Current statusMay 6, 2026Processing site: PDBe / Status: Released

-
Structure visualization

Supplemental images

emd_51914.png

Downloads & links

-
Map

FileDownload / File: emd_51914.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMap 3A around the atomic model
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.89 Å/pix.
x 350 pix.
= 310.625 Å		0.89 Å/pix.
x 350 pix.
= 310.625 Å		0.89 Å/pix.
x 350 pix.
= 310.625 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.8875 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0022
Minimum - Maximum-0.017639467 - 0.017730994
Average (Standard dev.)0.000006619054 (±0.00027589005)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions350350350
Spacing350350350
CellA=B=C: 310.625 Å
α=β=γ: 90.0 °

-
Supplemental data

-
Mask #1

Fileemd_51914_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Additional map: #1

Fileemd_51914_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: #2

Fileemd_51914_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Half map: #1

Fileemd_51914_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

-
Sample components

-
Entire : Nanofibrils from dual amphiphilic synthetic peptide

EntireName: Nanofibrils from dual amphiphilic synthetic peptide
Components
  • Complex: Nanofibrils from dual amphiphilic synthetic peptide
    • Protein or peptide: Peptide LYS-VAL-LYS-VAL-SER-GLN-ILE-ASN-MET

-
Supramolecule #1: Nanofibrils from dual amphiphilic synthetic peptide

SupramoleculeName: Nanofibrils from dual amphiphilic synthetic peptide / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: other sequences (unknown)

-
Macromolecule #1: Peptide LYS-VAL-LYS-VAL-SER-GLN-ILE-ASN-MET

MacromoleculeName: Peptide LYS-VAL-LYS-VAL-SER-GLN-ILE-ASN-MET / type: protein_or_peptide / ID: 1 / Number of copies: 54 / Enantiomer: LEVO
Source (natural)Organism: other sequences (unknown)
Molecular weightTheoretical: 1.048299 KDa
SequenceString:
KVKVSQINM

-
Experimental details

-
Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation statehelical array

-
Sample preparation

Concentration1 mg/mL
BufferpH: 7.4
GridModel: Quantifoil R2/1 / Material: COPPER / Mesh: 400 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

-
Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOCONTINUUM (6k x 4k) / Number grids imaged: 1 / Average electron dose: 39.28839 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 4.77 Å
Applied symmetry - Helical parameters - Δ&Phi: -0.64 °
Applied symmetry - Helical parameters - Axial symmetry: C6 (6 fold cyclic)
Resolution.type: BY AUTHOR / Resolution: 2.96 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0) / Number images used: 108623
CTF correctionSoftware - Name: MotionCorr2 / Type: PHASE FLIPPING ONLY
Startup modelType of model: OTHER / Details: Featureless Cylinder created with Relion
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more