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- EMDB-51911: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guide... -

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Basic information

Entry
Database: EMDB / ID: EMD-51911
TitleLate-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA
Map dataMain map of the mammalian late-stage 48S initiation complex with eIF3 (LS48S-eIF3 IC)
Sample
  • Complex: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA of 15 nt length, purification method of biotinylated DNA-oligo pull-down
    • RNA: x 3 types
    • Protein or peptide: x 48 types
  • Ligand: x 1 types
Keywords48S late-stage initiation complex / Programmable mRNA translation initiation / trans-RNA / activation of mRNA translation / TRANSLATION
Function / homology
Function and homology information


regulation of macromolecule metabolic process / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / eukaryotic initiation factor eIF2 binding / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / viral translational termination-reinitiation / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane ...regulation of macromolecule metabolic process / Translation initiation complex formation / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / eukaryotic initiation factor eIF2 binding / Major pathway of rRNA processing in the nucleolus and cytosol / GTP hydrolysis and joining of the 60S ribosomal subunit / viral translational termination-reinitiation / L13a-mediated translational silencing of Ceruloplasmin expression / SRP-dependent cotranslational protein targeting to membrane / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / eukaryotic translation initiation factor 3 complex, eIF3e / cap-dependent translational initiation / eukaryotic translation initiation factor 3 complex, eIF3m / IRES-dependent viral translational initiation / translation reinitiation / eukaryotic translation initiation factor 2 complex / formation of cytoplasmic translation initiation complex / eukaryotic translation initiation factor 3 complex / multi-eIF complex / translation factor activity, RNA binding / eukaryotic 43S preinitiation complex / formation of translation preinitiation complex / mRNA cap binding / eukaryotic 48S preinitiation complex / cellular response to chemical stress / protein-synthesizing GTPase / metal-dependent deubiquitinase activity / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / regulation of translational initiation / GDP-dissociation inhibitor activity / Formation of the ternary complex, and subsequently, the 43S complex / laminin receptor activity / Ribosomal scanning and start codon recognition / Translation initiation complex formation / Formation of a pool of free 40S subunits / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ubiquitin ligase inhibitor activity / positive regulation of signal transduction by p53 class mediator / GTP hydrolysis and joining of the 60S ribosomal subunit / 90S preribosome / L13a-mediated translational silencing of Ceruloplasmin expression / phagocytic cup / laminin binding / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / translation regulator activity / ribosomal small subunit export from nucleus / gastrulation / translation initiation factor binding / MDM2/MDM4 family protein binding / translation initiation factor activity / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / GTPase activator activity / response to endoplasmic reticulum stress / positive regulation of translation / ribosome assembly / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / PML body / spindle / fibrillar center / cytoplasmic stress granule / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / regulation of translation / ribosome binding / virus receptor activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / perikaryon / cytoplasmic translation / cysteine-type deubiquitinase activity / cell differentiation / tRNA binding / mitochondrial inner membrane / rRNA binding / postsynaptic density / structural constituent of ribosome / ribosome / translation / ribonucleoprotein complex / cell division / DNA repair / mRNA binding / GTPase activity / apoptotic process / synapse / dendrite
Similarity search - Function
RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Possible Fer4-like domain in RNase L inhibitor, RLI / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F ...RLI, domain 1 / RLI1 / RNase L inhibitor RLI-like, possible metal-binding domain / Possible Fer4-like domain in RNase L inhibitor, RLI / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit 7 (eIF-3) / Eukaryotic translation initiation factor 3 subunit H / eIF3h, C-terminal / C-terminal region of eIF3h / Eukaryotic translation initiation factor 3 subunit F / Translation initiation factor 3 complex subunit L / RNA polymerase I-associated factor PAF67 / Eukaryotic translation initiation factor 3 subunit M / eIF3 subunit M, C-terminal helix domain / eIF3 subunit 6 N terminal domain / eIF3 subunit M, C-terminal helix / Eukaryotic translation initiation factor 3 subunit E, C-terminal / Eukaryotic translation initiation factor 3 subunit E / Eukaryotic translation initiation factor 3 subunit E, N-terminal / eIF3 subunit 6 N terminal domain / Eukaryotic translation initiation factor 3 subunit K / Translation initiation factor 3, subunit 12, N-terminal, eukaryotic / : / eIF3a, PCI domain, TPR-like region / Eukaryotic translation initiation factor 3 subunit A / Eukaryotic translation initiation factor 3 subunit C, N-terminal domain / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 3 subunit 8 N-terminus / Translation initiation factor IF2/IF5 domain / Translation initiation factor IF2/IF5, N-terminal / Translation initiation factor IF2/IF5, zinc-binding / Translation initiation factor IF2/IF5 / Domain found in IF2B/IF5 / domain present in translation initiation factor eIF2B and eIF5 / Eukaryotic translation initiation factor 3 subunit M eIF3m/COP9 signalosome complex subunit 7 COPS7 / eIF4-gamma/eIF5/eIF2-epsilon / Domain at the C-termini of GCD6, eIF-2B epsilon, eIF-4 gamma and eIF-5 / W2 domain / W2 domain profile. / Translation initiation factor 1A (eIF-1A), conserved site / Eukaryotic initiation factor 1A signature. / eukaryotic translation initiation factor 1A / Translation initiation factor 1A (eIF-1A) / Initiation factor eIF2 gamma, C-terminal / Initiation factor eIF2 gamma, domain 2 / Initiation factor eIF2 gamma, GTP-binding domain / Initiation factor eIF2 gamma, C terminal / Translation initiation factor 2, alpha subunit / Translation initiation factor 2, alpha subunit, middle domain superfamily / Translation initiation factor 2, alpha subunit, C-terminal / IF2a, S1-like domain / Eukaryotic translation initiation factor 2 alpha subunit / : / RNA-binding domain, S1, IF1 type / Translation initiation factor 1A / IF-1 / S1 domain IF1 type profile. / 4Fe-4S binding domain / CSN8/PSMD8/EIF3K / CSN8/PSMD8/EIF3K family / Rpn11/EIF3F, C-terminal / Maintenance of mitochondrial structure and function / : / motif in proteasome subunits, Int-6, Nip-1 and TRIP-15 / PCI domain / Proteasome component (PCI) domain / PCI domain profile. / 40S ribosomal protein SA / Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / JAB1/Mov34/MPN/PAD-1 ubiquitin protease / S1 domain profile. / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / : / Ribosomal protein S12e signature. / Ribosomal protein S12e / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S2, eukaryotic / Translation elongation factor EFTu-like, domain 2 / S27a-like superfamily / 40S Ribosomal protein S10 / Plectin/S10, N-terminal / Plectin/S10 domain / : / Ribosomal protein S7e signature.
Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / Eukaryotic translation initiation factor 3 subunit H / protein-synthesizing GTPase / ATP-binding cassette sub-family E member 1 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Eukaryotic translation initiation factor 3 subunit L / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 ...Small ribosomal subunit protein eS32 / Eukaryotic translation initiation factor 3 subunit H / protein-synthesizing GTPase / ATP-binding cassette sub-family E member 1 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS4 / Eukaryotic translation initiation factor 3 subunit L / Small ribosomal subunit protein eS12 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein RACK1 / Ubiquitin-ribosomal protein eS31 fusion protein / Eukaryotic translation initiation factor 3 subunit F / Eukaryotic translation initiation factor 3 subunit M / Eukaryotic translation initiation factor 3 subunit A / Small ribosomal subunit protein uS15 / Eukaryotic translation initiation factor 3 subunit D / Eukaryotic translation initiation factor 3 subunit E / Small ribosomal subunit protein eS7 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS11 / Eukaryotic translation initiation factor 2 subunit 1 / Eukaryotic translation initiation factor 3 subunit K / Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein / Small ribosomal subunit protein eS25 / Small ribosomal subunit protein eS26 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS8 / Eukaryotic translation initiation factor 5 / Small ribosomal subunit protein eS28 / Small ribosomal subunit protein eS8 / Small ribosomal subunit protein eS4 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein eS6 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS17 / 40S ribosomal protein S24 / Small ribosomal subunit protein eS17 / Small ribosomal subunit protein eS27 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS14 / Eukaryotic translation initiation factor 3 subunit C / Eukaryotic translation initiation factor 1A, X-chromosomal / Small ribosomal subunit protein eS21
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.9 Å
AuthorsNguyen TT / Hashem Y / Rocha REO / Boissier F / Qian SB / Jia L / Uematsu S / Gu Y / Shi S
Funding support United States, European Union, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)DP1GM142101 United States
Howard Hughes Medical Institute (HHMI)55108556 United States
European Research Council (ERC)101088541European Union
CitationJournal: Nat Biotechnol / Year: 2025
Title: Programmable initiation of mRNA translation by trans-RNA.
Authors: Longfei Jia / Tan-Trung Nguyen / Saori Uematsu / Yifei Gu / Shengcho Shi / Yaser Hashem / Shu-Bing Qian /
Abstract: Several approaches exist to silence genes, but few tools are available to activate individual mRNAs for translation inside cells. Guiding ribosomes to specific start codons without altering the ...Several approaches exist to silence genes, but few tools are available to activate individual mRNAs for translation inside cells. Guiding ribosomes to specific start codons without altering the original sequence remains a formidable task. Here we design capped trans-RNAs capable of directing ribosomes to specific initiation sites on individual mRNAs when the trans-cap is positioned near the target start codon. Structural and biochemical data suggest that the capped trans-RNA facilitates ribosome loading and scanning on the target mRNA through a synergistic mechanism involving alternative cap recognition. The trans-RNA also acts independently of the cap on the target mRNA, enabling translation of circular RNAs lacking internal ribosome entry sites. We apply trans-RNAs in vivo to achieve programmable alternative translation of endogenous genes in mouse liver. Finally, we provide the evidence for the existence of natural transcripts that, similarly to exogenous trans-RNAs, activate translation of endogenous mRNAs.
History
DepositionOct 25, 2024-
Header (metadata) releaseDec 10, 2025-
Map releaseDec 10, 2025-
UpdateDec 10, 2025-
Current statusDec 10, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51911.png

Downloads & links

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Map

FileDownload / File: emd_51911.map.gz / Format: CCP4 / Size: 476.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMain map of the mammalian late-stage 48S initiation complex with eIF3 (LS48S-eIF3 IC)
Projections & slices

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AxesZ (Sec.)Y (Row.)X (Col.)
0.93 Å/pix.
x 500 pix.
= 465. Å		0.93 Å/pix.
x 500 pix.
= 465. Å		0.93 Å/pix.
x 500 pix.
= 465. Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.93 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.15
Minimum - Maximum-0.6747698 - 1.156854
Average (Standard dev.)-0.00041077414 (±0.036055632)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions500500500
Spacing500500500
CellA=B=C: 465.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: EMhanced map of the mammalian late-stage 48S initiation...

Fileemd_51911_additional_1.map
AnnotationEMhanced map of the mammalian late-stage 48S initiation complex with eIF3 (LS48S-eIF3 IC)
Projections & Slices
AxesZYX

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Half map: Half map A of the mammalian late-stage 48S...

Fileemd_51911_half_map_1.map
AnnotationHalf map A of the mammalian late-stage 48S initiation complex with eIF3 (LS48S-eIF3 IC)
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AxesZYX

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Histogram

Histogram (log scale)

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Half map: Half map B of the mammalian late-stage 48S...

Fileemd_51911_half_map_2.map
AnnotationHalf map B of the mammalian late-stage 48S initiation complex with eIF3 (LS48S-eIF3 IC)
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Sample components

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Entire : Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guide...

EntireName: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA of 15 nt length, purification method of biotinylated DNA-oligo pull-down
Components
  • Complex: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA of 15 nt length, purification method of biotinylated DNA-oligo pull-down
    • RNA: Initiator methionylated tRNA
    • RNA: 18S ribosomal RNA
    • RNA: Linear mRNA
    • Protein or peptide: Eukaryotic translation initiation factor 2 subunit 1
    • Protein or peptide: protein-synthesizing GTPase
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Ribosomal protein S3
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: Ribosomal protein S5
    • Protein or peptide: Small ribosomal subunit protein eS6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: Small ribosomal subunit protein eS8
    • Protein or peptide: 40S ribosomal protein S9
    • Protein or peptide: Small ribosomal subunit protein eS10
    • Protein or peptide: 40S ribosomal protein S11
    • Protein or peptide: 40S ribosomal protein S12
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein uS11
    • Protein or peptide: 40S ribosomal protein uS19
    • Protein or peptide: Ribosomal protein S16
    • Protein or peptide: 40S ribosomal protein eS17
    • Protein or peptide: 40S ribosomal protein uS13
    • Protein or peptide: 40S ribosomal protein eS19
    • Protein or peptide: 40S ribosomal protein uS10
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: Ribosomal protein S15a
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: Ribosomal protein S27a
    • Protein or peptide: Small ribosomal subunit protein RACK1
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: Eukaryotic translation initiation factor 1A, X-chromosomal
    • Protein or peptide: ATP binding cassette subfamily E member 1
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit D
    • Protein or peptide: 40S ribosomal protein S25
    • Protein or peptide: Eukaryotic translation initiation factor 5
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit F
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit H
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit K
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit L
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit M
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit C
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit E
    • Protein or peptide: Eukaryotic translation initiation factor 3 subunit A
  • Ligand: IRON/SULFUR CLUSTER

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Supramolecule #1: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guide...

SupramoleculeName: Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA of 15 nt length, purification method of biotinylated DNA-oligo pull-down
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 1.5 MDa

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Macromolecule #1: Initiator methionylated tRNA

MacromoleculeName: Initiator methionylated tRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.721828 KDa
SequenceString:
GAGCAGAGUG GCGCAGCGGA AGCGUGCUGG GCCCAU(T6A)ACC CAGAGGUCGA UGGAUCGAAA CCAUCCUCUG CUACCA

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Macromolecule #2: 18S ribosomal RNA

MacromoleculeName: 18S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 601.015688 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC ...String:
UACCUGGUUG AUCCUGCCAG UAGCAUAUGC UUGUCUCAAA GAUUAAGCCA UGCAUGUCUA AGUACGCACG GCCGGUACAG UGAAACUGC GAAUGGCUCA UUAAAUCAGU UAUGGUUCCU UUGGUCGCUC GCUCCUCUCC UACUUGGAUA ACUGUGGUAA U UCUAGAGC UAAUACAUGC CGACGGCGCU GACUCCCUUU GUGUGGGAUG CGUGCAUUUA UCAGAUCAAA ACCAACCCGG UC AGCCUCC CCGCCGGCCG GGGGGGUGGG GCGGCGGCUU UGGUGACUCU AGAUAACCUC GGGCCGAUCG CAGCCCUCCG UGG CGGCGA CGACCCAUUC GAACGUCUGC CCUAUCAACU UUCGAUGGUA GUCGCCGUGC CUACCAUGGU GACCACGGGU GACG GGGAA UCAGGGUUCG AUUCCGGAGA GGGAGCCUGA GAAACGGCUA CCACAUCCAA GGAAGGCAGC AGGCGCGCAA AUUAC CCAC UCCCGACCCG GGGAGGUAGU GACGAAAAAU AACAAUACAG GACUCUUUCG AGGCCCUGUA AUUGGAAUGA GUCCAC UUU AAAUCCUUUA ACGAGGAUCC AUUGGAGGGC AAGUCUGGUC GCAGCAGCCG CGGUAAUUCC AGCUCCAAUA GCGUAUA UU AAAGUUGCUG CAGUUAAAAA GCUCGUAGUU GGAUCUUGUG GAGGGUGCGU AGCGGGCGGU CCGCCGCGAG GCGAGCCA C CGCCCGUCCC CGCCCCUUGC CUCUCGGCGC CCCCUCGAUG CUCUUAGCUG AGUGUCCCGC GGGGCCCGAA GCGUUUACU UUGAAAAAAU UAGAGUGUUC AAAGCAGGCC CGAGCCGCCU GGAUACCGCA GCUAGGAAUA AUGGAAUAGG ACCGCGGUUC UAUUUUGUU GGUUUUCGGA ACUGAGGCCA UGAUUAAGAG GGACGGCCGG GGGCAUUCGU AUUGCGCCGC UAGAGGUGAA A UUCUUGGA CCGGCGCAAG ACGGACCAGA GCGAAAGCAU UUGCCAAGAA UGUUUUCAUU AAUCAAGAAC GAAAGUCGGA GG UUCGAAG ACGAUCAGAU ACCGUCGUAG UUCCGACCAU AAACGAUGCC GACUGGCGAU GCGGCGGCGU UAUUCCCAUG ACC CGCCGG GCAGCUUCCG GGAAACCAAA GUCUUUGGGU UCCGGGGGGA GUAUGGUUGC AAAGCUGAAA CUUAAAGGAA UUGA CGGAA GGGCACCACC AGGAGUGGAG CCUGCGGCUU AAUUUGAC(I2T)C AACACGGGAA ACCUCACCCG GCCCGGACAC G GACAGGAU UGACAGAUUG AUAGCUCUUU CUCGAUUCUG UGGGUGGUGG UGCAUGGCCG UUCUUAGUUG GUGGAGCGAU UU GUCUGGU UAAUUCCGAU AACGAACGAG ACUCUGGCAU GCUAACUAGU UACGCGACCC CCGAGCGGUC GGCGUCCCCC AAC UUCUUA GAGGGACAAG UGGCGUUCAG CCACCCGAGA UUGAGCAAUA ACAGGUCUGU GAUGCCCUUA GAUGUCGGGG CUGC ACGCG CGCUACACUG ACUGGCUCAG CGUGUGCCUA CCCUACGCCG GCAGGCGCGG GUAACCCGUU GAACCCCAUU CGUGA UGGG GAUCGGGGAU UGCAAUUAUU CCCCAUGAAC GAGGAAUUCC CAGUAAGUGC GGGUCAUAAG CUUGCGUUGA UUAAGU CCC UGCCCUUUGU ACACACCGCC CGUCGCUACU ACCGAUUGGA UGGUUUAGUG AGGCCCUCGG AUCGGCCCGC CGGGGUC GG CCCACGGCCC UGGCGGAGCG CUGAGAAGAC GGUCGAACUU GACUAUCUAG AGGAAGUAAA AGUCGUAACA AGGUUUCC G UAGGUGAACC UGCGGAAGGA UCAUUA

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Macromolecule #3: Linear mRNA

MacromoleculeName: Linear mRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 51.665809 KDa
SequenceString:
GGGACAUUUG CUUCUGACAC AACAUGGAAU CGAUCAUCAA CUUCGAAAAG CUAUAGUAAU GUGUUCACUA GCAACCUCAA ACAGACACC GCCACCAUGG UGAGCGGCUG GCGGCUGUUC AAGAAGAUUA GCCCGCUCGA GAUGGUGAGC AAGGGCGAGG A

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Macromolecule #4: Eukaryotic translation initiation factor 2 subunit 1

MacromoleculeName: Eukaryotic translation initiation factor 2 subunit 1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 44.692543 KDa
SequenceString: MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL ...String:
MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRR VSPEEAIKCE DKFTKSKTVY SILRHVAEVL EYTKDEQLES LFQRTAWVFD DKYKRPGYGA YDAFKHAVSD P SILDSLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYGYEGIDAV KEALRAGLNC STETMPIKIN LIAPPRYVMT TT TLERTEG LSVLNQAMAV IKEKIEEKRG VFNVQMEQVW KTPRRKTLDL NEDEREVLIN NINRRLTPQA VKIRADIEVA CYG YEGIDA VKEALRAGLN CSTETMPIKI NLIAPPRYVM TTTTLERTEG LSVLNQAMAV IKEKIEEKRG VFNV

UniProtKB: Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 1

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Macromolecule #5: protein-synthesizing GTPase

MacromoleculeName: protein-synthesizing GTPase / type: protein_or_peptide / ID: 5 / Details: Uniprot ID: A0A5F9C3W2 / Number of copies: 1 / Enantiomer: LEVO / EC number: protein-synthesizing GTPase
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 52.499992 KDa
SequenceString: MRLPGQLPSS PGKMAGGEAG VTLGQPHLSR QDLATLDVTK LTPLSHEVIS RQATINIGTI GHVAHGKSTV VKAISGVHTV RFKNELERN ITIKLGYANA KIYKLDDPSC PRPECYRSCG SSTPDEFPTD IPGTKGNFKL VRHVSFVDCP GHDILMATML N GAAVMDAA ...String:
MRLPGQLPSS PGKMAGGEAG VTLGQPHLSR QDLATLDVTK LTPLSHEVIS RQATINIGTI GHVAHGKSTV VKAISGVHTV RFKNELERN ITIKLGYANA KIYKLDDPSC PRPECYRSCG SSTPDEFPTD IPGTKGNFKL VRHVSFVDCP GHDILMATML N GAAVMDAA LLLIAGNESC PQPQTSEHLA AIEIMKLKHI LILQNKIDLV KESQAKEQYE QILAFVQGTV AEGAPIIPIS AQ LKYNIEV VCEYIVKKIP VPPRDFTSEP RLIVIRSFDV NKPGCEVDDL KGGVAGGSIL KGVLKVGQEI EVRPGIVSKD SEG KLMCKP IFSKIVSLFA EHNDLQYAAP GGLIGVGTKI DPTLCRADRM VGQVLGAVGA LPEIFTELEI SYFLLRRLLG VRTE GDKKA AKVQKLSKNE VLMVNIGSLS TGGRVSAVKA DLGKIVLTNP VCTEVGEKIA LSRRVEKHWR LIGWGQIRRG VTIKP TVDD D

UniProtKB: protein-synthesizing GTPase

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Macromolecule #6: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 6 / Details: G1TLT8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 32.927988 KDa
SequenceString: MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA ...String:
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLK FAAATGATPI AGRFTPGTFT NQIQAAFREP RLLVVTDPRA DHQPLTEASY VNLPTIALCN TDSPLRYVDI A IPCNNKGA HSVGLMWWML AREVLRMRGT ISREHPWEVM PDLYFYRDPE EIEKEEQAAA EKAVTKEEFQ GEWTAPAPEF TA TQPEVAD WSEGVQVPSV PIQQFPTEDW SAQPATEDWS AAPTAQATEW VGTTTEWS

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #7: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 7 / Details: G1TN72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 29.928967 KDa
SequenceString: MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLHLFC VGFTKKCNNQ IRKTSYAQHQ Q VRQIRKKM ...String:
MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITE DVQGKNCLTN FHGMDLTRDK MCSMVKKWQT MIEAHVDVKT TDGYLLHLFC VGFTKKCNNQ IRKTSYAQHQ Q VRQIRKKM MEIMTREVQT NDLKEVVNKL IPDSIGKDIE KACQSIYPLH DVFVRKVKML KKPKFELGKL MELHGEGSSS GK ATGDETG AKVERADGYE PPVQESV

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #8: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 8 / Details: A0AAA9WW19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 29.099955 KDa
SequenceString: LGFGGAEGGG GGRLLGLGSG RGRGRGARGG KAEDKEWLPV TKLGRLVKDV KIKSLEEIYL FSLPIKESEI IDFFLGAALK DEVLKIMPV QKQTRAAQRT RFKAFVAIGD YNGHVGLGVK CSKEVATAIR GAIILAKLSI VPVRRGYWGN KIGKPHTVPC K VTGRCGSV ...String:
LGFGGAEGGG GGRLLGLGSG RGRGRGARGG KAEDKEWLPV TKLGRLVKDV KIKSLEEIYL FSLPIKESEI IDFFLGAALK DEVLKIMPV QKQTRAAQRT RFKAFVAIGD YNGHVGLGVK CSKEVATAIR GAIILAKLSI VPVRRGYWGN KIGKPHTVPC K VTGRCGSV LVHLIPAPRG TGIVSAPVPK KLLLMAGIDD CYTSAWSCTA TLGNFAKATF DAISKTYSYL TPDLWKETVF TK SPYQEFT DHLVKTHTRV SVQRTQAPAV ATT

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #9: Ribosomal protein S3

MacromoleculeName: Ribosomal protein S3 / type: protein_or_peptide / ID: 9 / Details: G1TNM3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 26.715344 KDa
SequenceString: MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV ...String:
MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAE KVATRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFIMES GAKGCEVVVS GKLRGQRAKS MKFVDGLMIH S GDPVNYYV DTAVRHVLLR QGVLGIKVKI MLPWDPSGKI GPKKPLPDHV SIVEPKDEIL PTTPISEQKG GKPEPPAMPQ PV PTA

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #10: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 10 / Details: G1TK17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 29.65892 KDa
SequenceString: MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ...String:
MARGPKKHLK RVAAPKHWML DKLTSVFAPR PSTGPHKLRE CLPLIIFLRN KLKYALTGDE VKKICMQRFI KIDGKVRADI TYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKMNDT I QIDLETGK ITDFIKFDTG NLCMVTGGAN LGRIGVITNR ERHPGSFDVV HVKDANGNSF ATRLSNIFVI GKGNKPWISL PR GKGIRLT IAEERDKRLA AKQSSG

UniProtKB: Small ribosomal subunit protein eS4

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Macromolecule #11: Ribosomal protein S5

MacromoleculeName: Ribosomal protein S5 / type: protein_or_peptide / ID: 11 / Details: G1TFM5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.913453 KDa
SequenceString: MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT ...String:
MTEWETAAPA VAETPDIKLF GKWSTDDVQI NDISLQDYIA VKEKYAKYLP HSAGRYAAKR FRKAQCPIVE RLTNSMMMHG RNNGKKLMT VRIVKHAFEI IHLLTGENPL QVLVNAIINS GPREDSTRIG RAGTVRRQAV DVSPLRRVNQ AIWLLCTGAR E AAFRNIKT IAECLADELI NAAKGSSNSY AIKKKDELER VAKSNR

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #12: Small ribosomal subunit protein eS6

MacromoleculeName: Small ribosomal subunit protein eS6 / type: protein_or_peptide / ID: 12 / Details: G1TM55 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 28.755895 KDa
SequenceString: MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV TRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK ...String:
MKLNISFPAT GCQKLIEVDD ERKLRTFYEK RMATEVAADA LGEEWKGYVV RISGGNDKQG FPMKQGVLTH GRVRLLLSKG HSCYRPRRT GERKRKSVRG CIVDANLSVL NLVIVKKGEK DIPGLTDTTV TRRLGPKRAS RIRKLFNLSK EDDVRQYVVR K PLNKEGKK PRTKAPKIQR LVTPRVLQHK RRRIALKKQR TKKNKEEAAE YAKLLAKRMK EAKEKRQEQI AKRRRLSSLR AS TSKSESS QK

UniProtKB: Small ribosomal subunit protein eS6

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Macromolecule #13: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 13 / Details: G1SVB0 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.168914 KDa
SequenceString: MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH ...String:
MFSSSAKIVK PNGEKPDEFE SGISQALLEL EMNSDLKAQL RELNITAAKE IEVGGGRKAI IIFVPVPQLK SFQKIQVRLV RELEKKFSG KHVVFIAQRR ILPKPTRKSR TKNKQKRPRS RTLTAVHDAI LEDLVFPSEI VGKRIRVKLD GSRLIKVHLD K AQQNNVEH KVETFSGVYK KLTGKDVNFE FPEFQL

UniProtKB: Small ribosomal subunit protein eS7

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Macromolecule #14: Small ribosomal subunit protein eS8

MacromoleculeName: Small ribosomal subunit protein eS8 / type: protein_or_peptide / ID: 14 / Details: G1TJW1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 24.163244 KDa
SequenceString: MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVGVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ ...String:
MGISRDNWHK RRKTGGKRKP YHKKRKYELG RPAANTKIGP RRIHTVGVRG GNKKYRALRL DVGNFSWGSE CCTRKTRIID VVYNASNNE LVRTKTLVKN CIVLIDSTPY RQWYESHYAL PLGRKKGAKL TPEEEEILNK KRSKKIQKKY DERKKNAKIS S LLEEQFQQ GKLLACIASR PGQCGRADGY VLEGKELEFY LRKIKARKGK

UniProtKB: Small ribosomal subunit protein eS8

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Macromolecule #15: 40S ribosomal protein S9

MacromoleculeName: 40S ribosomal protein S9 / type: protein_or_peptide / ID: 15 / Details: B7NZS8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 22.641564 KDa
SequenceString: MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG ...String:
MPVARSWVCR KTYVTPRRPF EKSRLDQELK LIGEYGLRNK REVWRVKFTL AKIRKAAREL LTLDEKDPRR LFEGNALLRR LVRIGVLDE GKMKLDYILG LKIEDFLERR LQTQVFKLGL AKSIHHARVL IRQRHIRVRK QVVNIPSFIV RLDSQKHIDF S LRSPYGGG RPGRVKRKNA KKGQGGAGAG DDEEED

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #16: Small ribosomal subunit protein eS10

MacromoleculeName: Small ribosomal subunit protein eS10 / type: protein_or_peptide / ID: 16 / Details: G1U0Y5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.156865 KDa
SequenceString:
MLMPKKNRIA IYELLFKEGV MVAKKDVHMP KHPELADKNV PNLHVMKAMQ SLKSRGYVKE QFAWRHFYWY LTNEGIQYLR DYLHLPPEI VPATLRRSRP ETGRPRPKGL EGERPARLTR GEADRDTYRR SAVPPGADKK AEAGAGSATE

UniProtKB: Small ribosomal subunit protein eS10

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Macromolecule #17: 40S ribosomal protein S11

MacromoleculeName: 40S ribosomal protein S11 / type: protein_or_peptide / ID: 17 / Details: G1TRM4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.468826 KDa
SequenceString:
MADIQTERAY QKQPTIFQNK KRVLLGETGK EKLPRYYKNI GLGFKTPKEA IEGTYIDKKC PFTGNVSIRG RILSGVVTKM KMQRTIVIR RDYLHYIRKY NRFEKRHKNM SVHLSPCFRD VQIGDIVTVG ECRPLSKTVR FNVLKVTKAA GTKKQFQKF

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #18: 40S ribosomal protein S12

MacromoleculeName: 40S ribosomal protein S12 / type: protein_or_peptide / ID: 18 / Details: G1SFR8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.538987 KDa
SequenceString:
MAEEGIAAGG VMDVNTALQE VLKTALIHDG LARGIREAAK ALDKRQAHLC VLASNCDEPM YVKLVEALCA EHQINLIKVD DNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK

UniProtKB: Small ribosomal subunit protein eS12

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Macromolecule #19: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 19 / Details: G1SP51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.259389 KDa
SequenceString:
MGRMHAPGKG LSQSALPYRR SVPTWLKLTS DDVKEQIYKL AKKGLTPSQI GVILRDSHGV AQVRFVTGNK ILRILKSKGL APDLPEDLY HLIKKAVAVR KHLERNRKDK DAKFRLILIE SRIHRLARYY KTKRVLPPNW KYESSTASAL VA

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #20: 40S ribosomal protein uS11

MacromoleculeName: 40S ribosomal protein uS11 / type: protein_or_peptide / ID: 20 / Details: G1T1F0 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.302772 KDa
SequenceString:
MAPRKGKEKK EEQVISLGPQ VAEGENVFGV CHIFASFNDT FVHVTDLSGK ETICRVTGGM KVKADRDESS PYAAMLAAQD VAQRCKELG ITALHIKLRA TGGNRTKTPG PGAQSALRAL ARSGMKIGRI EDVTPIPSDS TRRKGGRRGR RL

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #21: 40S ribosomal protein uS19

MacromoleculeName: 40S ribosomal protein uS19 / type: protein_or_peptide / ID: 21 / Details: G1U0Q2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.049182 KDa
SequenceString:
MAEVEQKKKR TFRKFTYRGV DLDQLLDMSY EQLMQLYSAR QRRRLSRGLR RKQHSLLKRL RKAKKEAPPM EKPEVVKTHL RDMIILPEM VGSMVGVYNG KTFNQVEIKP EMIGHYLGEF SITYKPVKHG RPGIGATHSS RFIPLK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #22: Ribosomal protein S16

MacromoleculeName: Ribosomal protein S16 / type: protein_or_peptide / ID: 22 / Details: G1SGX4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.477377 KDa
SequenceString:
MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK ERFAGVDIRV RVKGGGHVAQ IYAIRQSIS KALVAYYQKY VDEASKKEIK DILIQYDRTL LVADPRRCES KKFGGPGARA RYQKSYR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #23: 40S ribosomal protein eS17

MacromoleculeName: 40S ribosomal protein eS17 / type: protein_or_peptide / ID: 23 / Details: G1TU13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.552119 KDa
SequenceString:
MGRVRTKTVK KAARVIIEKY YTRLGNDFHT NKRVCEEIAI IPSKKLRNKI AGYVTHLMKR IQRGPVRGIS IKLQEEERER RDNYVPEVS ALDQEIIEVD PDTKEMLKLL DFGSLSNLQV TQPTVGMNFK TPRGAV

UniProtKB: Small ribosomal subunit protein eS17

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Macromolecule #24: 40S ribosomal protein uS13

MacromoleculeName: 40S ribosomal protein uS13 / type: protein_or_peptide / ID: 24 / Details: G1TPG3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 17.759777 KDa
SequenceString:
MSLVIPEKFQ HILRVLNTNI DGRRKIAFAI TAIKGVGRRY AHVVLRKADI DLTKRAGELT EDEVERVITI MQNPRQYKIP DWFLNRQKD VKDGKYSQVL ANGLDNKLRE DLERLKKIRA HRGLRHFWGL RVRGQHTKTT GRRGRTVGVS KKK

UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #25: 40S ribosomal protein eS19

MacromoleculeName: 40S ribosomal protein eS19 / type: protein_or_peptide / ID: 25 / Details: G1TN62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.235796 KDa
SequenceString:
MPGVTVKDVN QQEFVRALAA FLKKSGKLKV PEWVDTVKLA KHKELAPYDE NWFYTRAAST ARHLYLRGGA GVGSMTKIYG GRQRNGVMP SHFSRGSKSV ARRVLQALEG LKMVEKDQDW GRKLTPQGQR DLDRIAGQVA AAKKKH

UniProtKB: Small ribosomal subunit protein eS19

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Macromolecule #26: 40S ribosomal protein uS10

MacromoleculeName: 40S ribosomal protein uS10 / type: protein_or_peptide / ID: 26 / Details: G1SIZ2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.398763 KDa
SequenceString:
MAFKDTGKTP VEPEVAIHRI RITLTSRNVK SLEKVCADLI RGAKEKNLKV KGPVRMPTKT LRITTRKTPC GEGSKTWDRF QMRIHKRLI DLHSPSEIVK QITSISIEPG VEVEVTIADA

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #27: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 27 / Details: G1TM82 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.124389 KDa
SequenceString:
MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF

UniProtKB: Small ribosomal subunit protein eS21

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Macromolecule #28: Ribosomal protein S15a

MacromoleculeName: Ribosomal protein S15a / type: protein_or_peptide / ID: 28 / Details: G1TG89 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.865555 KDa
SequenceString:
MVRMNVLADA LKSINNAEKR GKRQVLIRPC SKVIVRFLTV MMKHGYIGEF EIIDDHRAGK IVVNLTGRLN KCGVISPRFD VQLKDLEKW QNNLLPSRQF GFIVLTTSAG IMDHEEARRK HTGGKILGFF F

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #29: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 29 / Details: G1SZ47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.844666 KDa
SequenceString:
MGKCRGLRTA RKLRSHRRDQ KWHDKQYKKA HLGTALKANP FGGASHAKGI VLEKVGVEAK QPNSAIRKCV RVQLIKNGKK ITAFVPNDG CLNFIEENDE VLVAGFGRKG HAVGDIPGVR FKVVKVANVS LLALYKGKKE RPRS

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #30: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 30 / Details: G1TS40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 15.434267 KDa
SequenceString:
MNDTVTIRTR KFMTNRLLQR KQMVIDVLHP GKATVPKTEI QEKLAKMYKT TPDVIFVFGF RTHFGGGKTT GFGMIYDSLD YAKKNEPKH RLARHGLYEK KKTSRKQRKE RKNRMKKVRG TAKANVGAGK KPKE

UniProtKB: 40S ribosomal protein S24

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Macromolecule #31: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 31 / Details: G1TFE8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 12.961455 KDa
SequenceString:
MTKKRRNNGR AKKGRGHVQP IRCTNCACCV PKDKAIKKFV IRNIVEAAAV RDISEVSVFD AYVLPKLYVK LHYCVSCAIH SKVVRNRSR EARKDRTPPP RFRPAGAAPP PPPKPM

UniProtKB: Small ribosomal subunit protein eS26

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Macromolecule #32: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 32 / Details: G1TZ76 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 9.480186 KDa
SequenceString:
MPLAKDLLHP SPEEEKRKHK KKRLVQSPNS YFMDVKCPGC YKITTVFSHA QTVVLCVGCS TVLCQPTGGK ARLTEGCSFR RKQH

UniProtKB: Small ribosomal subunit protein eS27

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Macromolecule #33: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 33 / Details: G1TIB4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 7.855052 KDa
SequenceString:
MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE SEREARRLR

UniProtKB: Small ribosomal subunit protein eS28

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Macromolecule #34: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 34 / Details: G1U7M4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 6.690821 KDa
SequenceString:
MGHQQLYWSH PRKFGQGSRS CRVCSNRHGL IRKYGLNMCR QCFRQYAKDI GFIKLD

UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #35: Ribosomal protein S27a

MacromoleculeName: Ribosomal protein S27a / type: protein_or_peptide / ID: 35 / Details: G1SK22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 18.004041 KDa
SequenceString:
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGGAKKR KKKSYTTPK KNKHKRKKVK LAVLKYYKVD ENGKISRLRR ECPSDECGAG VFMASHFDRH YCGKCCLTYC FNKPEDK

UniProtKB: Ubiquitin-ribosomal protein eS31 fusion protein

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Macromolecule #36: Small ribosomal subunit protein RACK1

MacromoleculeName: Small ribosomal subunit protein RACK1 / type: protein_or_peptide / ID: 36 / Details: G1SJB4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 35.115652 KDa
SequenceString: MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC ...String:
MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR GHSHFVSDVV ISSDGQFALS GSWDGTLRL WDLTTGTTTR RFVGHTKDVL SVAFSSDNRQ IVSGSRDKTI KLWNTLGVCK YTVQDESHSE WVSCVRFSPN S SNPIIVSC GWDKLVKVWN LANCKLKTNH IGHTGYLNTV TVSPDGSLCA SGGKDGQAML WDLNEGKHLY TLDGGDIINA LC FSPNRYW LCAATGPSIK IWDLEGKIIV DELKQEVIST SSKAEPPQCT SLAWSADGQT LFAGYTDNLV RVWQVTIGTR

UniProtKB: Small ribosomal subunit protein RACK1

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Macromolecule #37: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 37 / Details: G1T8A2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 14.498884 KDa
SequenceString:
MQLFVRAQEL HTLEVTGRET VAQIKAHVAS LEGIAPEDQV VLLAGTPLED EATLGQCGVE ALSTLEVAGR MLGGKVHGSL ARVGKVRGQ TLKVAKQEKK KKRTGRAKRR MQYNRRFVNV VPTFGKKKGP NANS

UniProtKB: Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein

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Macromolecule #38: Eukaryotic translation initiation factor 1A, X-chromosomal

MacromoleculeName: Eukaryotic translation initiation factor 1A, X-chromosomal
type: protein_or_peptide / ID: 38
Details: Uniprot: G1SYS4, Eukaryotic translation initiation factor 4C. Many last papers called this protein as Eukaryotic translation initiation factor 1A
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 16.476395 KDa
SequenceString:
MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKA DVILKYNADE ARSLKAYGEL PEHAKINETD TFGPGDDDEI QFDDIGDDDE DTDDI

UniProtKB: Eukaryotic translation initiation factor 1A, X-chromosomal

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Macromolecule #39: ATP binding cassette subfamily E member 1

MacromoleculeName: ATP binding cassette subfamily E member 1 / type: protein_or_peptide / ID: 39 / Details: G1SG72 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 67.419258 KDa
SequenceString: MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCA NAFKLHRLPI PRPGEVLGLV GTNGIGKSTA LKILAGKQKP NLGKYDDPPD WQEILTYFRG SELQNYFTKI L EDDLKAII ...String:
MADKLTRIAI VNHDKCKPKK CRQECKKSCP VVRMGKLCIE VTPQSKIAWI SETLCIGCGI CIKKCPFGAL SIVNLPSNLE KETTHRYCA NAFKLHRLPI PRPGEVLGLV GTNGIGKSTA LKILAGKQKP NLGKYDDPPD WQEILTYFRG SELQNYFTKI L EDDLKAII KPQYVDQIPK AAKGTVGSIL DRKDETKTQA IVCQQLDLTH LKERNVEDLS GGELQRFACA VVCIQKADIF MF DEPSSYL DVKQRLKAAI TIRSLINPDR YIIVVEHDLS VLDYLSDFIC CLYGVPSAYG VVTMPFSVRE GINIFLDGYV PTE NLRFRD ASLVFKVAET ANEEEVKKMC MYKYPGMKKK MGEFELAIVA GEFTDSEIMV MLGENGTGKT TFIRMLAGRL KPDE GGEVP VLNVSYKPQK ISPKSTGSVR QLLHEKIRDA YTHPQFVTDV MKPLQIENII DQEVQTLSGG ELQRVALALC LGKPA DVYL IDEPSAYLDS EQRLMAARVV KRFILHAKKT AFVVEHDFIM ATYLADRVIV FDGIPSKNTV ANSPQTLLAG MNKFLS QLE ITFRRDPNNY RPRINKLNSI KDVEQKKSGN YFFLDD

UniProtKB: ATP-binding cassette sub-family E member 1

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Macromolecule #40: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 40 / Details: A0A087WNH4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

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Macromolecule #41: Eukaryotic translation initiation factor 3 subunit D

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit D / type: protein_or_peptide / ID: 41 / Details: G1SQV5 (162-526) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 64.959684 KDa
SequenceString: MVQGRGSEKM AKFLTPVIQD NPSGWGPCAV PEQFRDMPYQ PFSKGDRLGK VADWTGATYQ DKRYTNKYSS QFGGGSQYAY FHEEDETSF QLVDTARTQK TAYQRNRMRF AQRNLRRDKD RRNMLQFNLQ TLPKSAKQKE RERIRLQKKF QKQFGVRQKW D QKSQKPRD ...String:
MVQGRGSEKM AKFLTPVIQD NPSGWGPCAV PEQFRDMPYQ PFSKGDRLGK VADWTGATYQ DKRYTNKYSS QFGGGSQYAY FHEEDETSF QLVDTARTQK TAYQRNRMRF AQRNLRRDKD RRNMLQFNLQ TLPKSAKQKE RERIRLQKKF QKQFGVRQKW D QKSQKPRD SSVEVRSDWE VKEEMDFPQL LKMRYLEVSE PQDIECCGAL EYYDKAFDRI TTRSEKPLRS IKRIFHTVTT TD DPVIRKL AKTQGNVFAT DAILATLMSC TRSVYSWDIV VQRVGSKLFF DKRDNSDFDL LTVSETANEP PQDEGNSFNS PRN LAMEAT YINHNFSQQC LRMGKERYNF PNPNPFVEDD MDKNEIASVA YRYRRWKLGD DIDLIVRCEH DGVMTGANGE VSFI NIKTL NEWDSRHCNG VDWRQKLDSQ RGAVIATELK NNSYKLARWT CCALLAGSEY LKLGYVSRYH VKDSSRHVIL GTQQF KPNE FASQINLSVE NAWGILRCVI DICMKLEEGK YLILKDPNKQ VIRVYSLPDG TFSSDEDDDD EEEEEEEEEE EET

UniProtKB: Eukaryotic translation initiation factor 3 subunit D

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Macromolecule #42: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 42 / Details: G1TDB3 (41-115) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 13.645028 KDa
SequenceString:
PPKDDKKKKD AGKSAKKDKD PVNKSGGKAK KKKWSKGKVR DKLNNLVLFD KATYDKLCKE VPNYKLITPA VVSERLKIRG SLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA AGEDA

UniProtKB: Small ribosomal subunit protein eS25

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Macromolecule #43: Eukaryotic translation initiation factor 5

MacromoleculeName: Eukaryotic translation initiation factor 5 / type: protein_or_peptide / ID: 43
Details: G1TGW1 (2019-12-11 v2) (430 aa) and A0A5F9C5D3 (2019-12-11 v1) (398 aa). Different the C-ter of two protein isoform from the position 354
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 49.13152 KDa
SequenceString: MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDML DGFIKKFVLC PECENPETDL HVNPKKQTIG NSCKACGYRG MLDTHHKLCT FILKNPPENS DSATGKKEKE K KNRKGKDK ...String:
MSVNVNRSVS DQFYRYKMPR LIAKVEGKGN GIKTVIVNMV DVAKALNRPP TYPTKYFGCE LGAQTQFDVK NDRYIVNGSH EANKLQDML DGFIKKFVLC PECENPETDL HVNPKKQTIG NSCKACGYRG MLDTHHKLCT FILKNPPENS DSATGKKEKE K KNRKGKDK ENGSVSSSET PPPPPPNEIS PPPHAVEEEE DDDWGEDTTE EAQRRRMDEI SDHAKVLTLS DDLERTVEER VN ILFDFVK KKKEEGVIDS SDKEIVAEAE RLDVKAMGPL VLTEVLFNEK IREQIKKYRR HFLRFCHNNK KAQRYLLHGL ECV VAMHQA QLISKIPHIL KEMYDADLLE EEVIISWSEK ASKKYVSKEL AKEIRVKAEP FIKWLKEAEE ESSGGEEDDE DENI EVVYS KTASVPKVET VKSDNKDDDI DIDAI

UniProtKB: Eukaryotic translation initiation factor 5

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Macromolecule #44: Eukaryotic translation initiation factor 3 subunit F

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit F / type: protein_or_peptide / ID: 44 / Details: G1SLC2 (364 aa) (86-357) A0A5F9CZR4 (366 aa) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 37.84673 KDa
SequenceString: MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK ...String:
MATPAVSASA PPATPAATPA AAAAPASAPA AAPAPTPAPA PAAAPAAGAA PAAAADPAAA AAAAGTGQTP ASAQAPAQTS APSLPGPAL PGPFPGGRVV RLHPVILASI VDSYERRNEG AARVIGTLLG TVDKHSVEVT NCFSVPHNES EDEVAVDMEF A KNMYELHK KVSPNELILG WYATGHDITE HSVLIHEYYS REAPNPIHLT VDTSLQNGRM SIKAYVSTSM GVPGRTMGVM FT PLTVKYA YYDTERIGVD LIMKTCFSPN RVIGLSSDLQ QVGGASARIQ DALSTVLQYA EDVLSGKVSA DNTVGRFLMS LVN QVPKIV PDDFETMLNS NINDLLMVTY LANLTQSQIA LNEKLVNL

UniProtKB: Eukaryotic translation initiation factor 3 subunit F

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Macromolecule #45: Eukaryotic translation initiation factor 3 subunit H

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit H / type: protein_or_peptide / ID: 45 / Details: A0A1U7U441 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 39.955324 KDa
SequenceString: MASRKEGAGP NAVPSSSATG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR ...String:
MASRKEGAGP NAVPSSSATG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTE DDADFDEVQY QMEMMRSLRH VNIDHLHVGW YQSTYYGSFV TRALLDSQFS YQHAIEESVV LIYDPIKTAQ G SLSLKAYR LTPKLMEVCK EKDFSPEALK KANITFEHMF EEVPIVIKNS HLINVLMWEL EKKSAVADKH ELLSLASSNH LG KNLQLLM DRVDEMSQDI VKYNTYMRNT SKQQQQKHQY QQRRQQENMQ RQSRGEPPLP EEDLSKLFKP PQPPARMDSL LIA GQINTY CQNIKEFTAQ NLGKLFMAQA LQEYNN

UniProtKB: Eukaryotic translation initiation factor 3 subunit H

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Macromolecule #46: Eukaryotic translation initiation factor 3 subunit K

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit K / type: protein_or_peptide / ID: 46 / Details: G1T3L2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 25.129709 KDa
SequenceString: MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS ...String:
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMI DQAHQEERPI RQILYLGDLL ETCHFQAFWQ ALDENMDLLE GITGFEDSVR KFICHVVGIT YQHIDRWLLA E MLGDLTDS QLKMWMSKYG WSADESGQIF ICSQEESIKP KNIVEKIDFD SVSSIMASSQ

UniProtKB: Eukaryotic translation initiation factor 3 subunit K

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Macromolecule #47: Eukaryotic translation initiation factor 3 subunit L

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit L / type: protein_or_peptide / ID: 47 / Details: G1SED9 (607 aa) (181-552) A0A5F9DP35 (466 aa) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 71.001477 KDa
SequenceString: PAGGCAQTSA FPAGPRRAEL PAPRRTRGSL SSLSPAVFAS AAAMSYPADD YESEAVYDPY AYPSDYDMHT GDPKQDLAYE RQYEQQTYQ VIPEVIRNFI QYFHKTVSDL IDQKVYELQA SRVSSDVIDQ KVYEIQDIYE NSWTKLTERF FKNTPWPEAE T IAPQVGND ...String:
PAGGCAQTSA FPAGPRRAEL PAPRRTRGSL SSLSPAVFAS AAAMSYPADD YESEAVYDPY AYPSDYDMHT GDPKQDLAYE RQYEQQTYQ VIPEVIRNFI QYFHKTVSDL IDQKVYELQA SRVSSDVIDQ KVYEIQDIYE NSWTKLTERF FKNTPWPEAE T IAPQVGND AVFLILYKEL YYRHIYAKVS GGPSLAQRFE SYYNYCNLFN YILNADGPAP LELPNQWLWD IIDEFIYQFQ SF SQYRCKT AKKSEEEIDF LRSNPKIWNV HSVLNVLHSL VDKSNINRQL EVYTSGGDPE SVAGEYGRHS LYKMLGYFSL VGL LRLHSL LGDYYQAIKV LENIELNKKS MYSRVPECQV TTYYYVGFAY LMMRRYQDAI RVFANILLYI QRTKSMFQRT TYKY EMINK QNEQMHALLA IALTMYPMRI DESIHLQLRE KYGDKMLRMQ KGDPQVYEEL FSYSCPKFLS PVVPNYDSVH PNYHK EPFL QQLKVFSDEV QQQAQLSTIR SFLKLYTTMP VAKLAGFLDL TEQEFRIQLL VFKHKMKNLV WTSGISALDG EFQSAS EVD FYIDKDMIHI ADTKVARRYG DFFIRQIHKF EELNRTLKKM GQRP

UniProtKB: Eukaryotic translation initiation factor 3 subunit L

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Macromolecule #48: Eukaryotic translation initiation factor 3 subunit M

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit M / type: protein_or_peptide / ID: 48 / Details: G1SLW8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 42.555832 KDa
SequenceString: MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL ...String:
MSVPAFIDIS EEDQAAELRA YLKSKGAEIS EENSEGGLHV DLAQIIEACD VCLKEDDKDV ESVMNSVVSL LLILEPDKQE ALIESLCEK LVKFREGERP SLRLQLLSNL FHGMDKNTPV RYTVYCSLIK VAASCGAIQY IPTELDQVRK WISDWNLTTE K KHTLLRLL YEALVDCKKS DAASKVMVEL LGSYTEDNAS QARVDAHRCI VRALKDPNAF LFDHLLTLKP VKFLEGELIH DL LTIFVSA KLASYVKFYQ NNKDFIDSLG LLHEQNMAKM RLLTFMGMAV ENKEISFDTM QQELQIGADD VEAFVIDAVR TKM VYCKID QTQRKVVVSH STHRTFGKQQ WQQLYDTLNA WKQNLNKVKN SLLSLSDT

UniProtKB: Eukaryotic translation initiation factor 3 subunit M

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Macromolecule #49: Eukaryotic translation initiation factor 3 subunit C

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit C / type: protein_or_peptide / ID: 49
Details: G1U971 (913 aa) A0A5F9CMJ3 (892 aa). The difference is in N-terminal of two version of protein Eukaryotic translation initiation factor 3 subunit C (A0A5F9CMJ3 - 892 aa - position 1-10 vs ...Details: G1U971 (913 aa) A0A5F9CMJ3 (892 aa). The difference is in N-terminal of two version of protein Eukaryotic translation initiation factor 3 subunit C (A0A5F9CMJ3 - 892 aa - position 1-10 vs G1U971 - 913 aa - position position 1-31)
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 105.676172 KDa
SequenceString: MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITNY K QNPEQSAD ...String:
MSRFFTTGSD SESESSLSGE ELVTKPVGGN YGKQPLLLSE DEEDTKRVVR SAKDKRFEEL TNLIRTIRNA MKIRDVTKCL EEFELLGKA YGKAKSIVDK EGVPRFYIRI LADLEDYLNE LWEDKEGKKK MNKNNAKALS TLRQKIRKYN RDFESHITNY K QNPEQSAD EDAEKNEEDS EGSSDEDEDE DGVSAAAFLK KKAEAPSGES RKFLKKMEDE DEDSEDSEDD EEWDTGSTSS DS DSEEEEG KQTALASRFL KKAPTTEEDK KAAEKKREDK AKKKHDRKSK RLDEEEEDNE GGEWERVRGG VPLVKEKPKM FAK GTEITH AVVIKKLNEI LQARGKKGTD RAAQIELLQL LVQIASENNL GEGVIVKIKF NIIASLYDYN PNLATYMKPE MWQK CLDCI NELMDILFAN PNIFVGENIL EESENLQNVD QPLRVRGCIL TLVERMDEEF TKIMQNTDPH SQEYVEHLKD EAQVC AIIE RVQRYLEEKG TTEEICRVYL RRILHTYYKF DYKAHQRQLT PPEGSSKSEQ DQAENEGEDS AVLMERLCKY IYAKDR TDR IRTCAILCHI YHHALHSRWY QARDLMLMSH LQDNIQHADP PVQILYNRTM VQLGICAFRQ GLTKDAHNAL LDIQSSG RA KELLGQGLLL RSLQERNQEQ EKVERRRQVP FHLHINLELL ECVYLVSAML LEIPYMAAHE SDARRRMISK QFHHQLRV G ERQPLLGPPE SMREHVVAAS KAMKMGDWKT CHSFIINEKM NGKVWDLFPE ADKVRTMLVR KIQEESLRTY LFTYSSVYD SISMETLSDM FELDLPTVHS IISKMIINEE LMASLDQPTQ TVVMHRTEPT AQQNLALQLA EKLGSLVENN ERVFDHKQGT YGGYFRDQK DGYRKNEGYM RRGGYRQQQS QTAY

UniProtKB: Eukaryotic translation initiation factor 3 subunit C

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Macromolecule #50: Eukaryotic translation initiation factor 3 subunit E

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit E / type: protein_or_peptide / ID: 50 / Details: G1SUC8 (4-422) / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 54.199863 KDa
SequenceString: MLKIFTCNSH SDLPTNLMAE YDLTTRIAHF LDRHLVFPLL EFLSVKEIYN EKELLQGKLD LLSDTNMVDF AMDVYKNLYS DDIPHALRE KRTTVVAQLK QLQAETEPIV KMFEDPETTR QMQSTRDGRM LFDYLADKHG FRQEYLDTLY RYAKFQYECG N YSGAAEYL ...String:
MLKIFTCNSH SDLPTNLMAE YDLTTRIAHF LDRHLVFPLL EFLSVKEIYN EKELLQGKLD LLSDTNMVDF AMDVYKNLYS DDIPHALRE KRTTVVAQLK QLQAETEPIV KMFEDPETTR QMQSTRDGRM LFDYLADKHG FRQEYLDTLY RYAKFQYECG N YSGAAEYL YFFRVLVPAT DRNALSSLWG KLASEILMQN WDAAMEDLTR LKETIDNNSV SSPLQSLQQR TWLIHWSLFV FF NHPKGRD NIIDLFLYQP QYLNAIQTMC PHILRYLTTA VITNKDVRKR RQVLKDLVKV IQQESYTYKD PITEFVECLY VNF DFDGAQ KKLRECESVL VNDFFLVACL EDFIENARLF IFETFCRIHQ CISINMLADK LNMTPEEAER WIVNLIRNAR LDAK IDSKL GHVVMGNNAV SPYQQVIEKT KSLSFRSQML AMNIEKKLNQ NSRSEAPNWA TQDSGFY

UniProtKB: Eukaryotic translation initiation factor 3 subunit E

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Macromolecule #51: Eukaryotic translation initiation factor 3 subunit A

MacromoleculeName: Eukaryotic translation initiation factor 3 subunit A / type: protein_or_peptide / ID: 51
Details: G1SMZ5 (1362 aa) A0A5F9C991 (1350 aa) A0A5F9CMG0 (1343 aa) A0A5F9C3S1 (1351 aa) The difference is in N-terminal of two version of protein Eukaryotic translation initiation factor 3 subunit A
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 164.902656 KDa
SequenceString: MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS ...String:
MPAYFQRPEN ALKRANEFLE VGKKQPALDV LYDVMKSKKH RTWQKIHEPI MLKYLELCVD LRKSHLAKEG LYQYKNICQQ VNIKSLEDV VRAYLKLAEE KTEAAKEESQ QMVLDIEDLD NIQTPESVLL SAVSGEDTQD RTDRLLLTPW VKFLWESYRQ C LDLLRNNS RVERLYHDIA QQAFKFCLQY TRKAEFRKLC DNLRMHLSQI QRHHNQSTAI NLNNPESQSM HLETRLVQLD SA ISMELWQ EAFKAVEDIH GLFSLSKKPP KPQLMANYYN KVSTVFWKSG NALFHASTLH RLYHLSREMR KNLTQDEMQR MST RVLLAT LSIPITPERT DIARLLDMDG IIVEKQRRLA TLLGLQAPPT RIGLINDMVR FNVLQYVVPE VKDLYNWLEV EFNP LKLCE RVTKVLNWVR EQPEKEPELQ QYVPQLQSNT ILRLLQQVAQ IYQSIEFSRL TSLVPFVDAF QLERAIVDAA RHCDL QVRI DHTSRTLSFG SDLNYATRED APIGPHLQSM PSEQIRNQLT AMSSVLAKAL EVIKPAHILQ EKEEQHQLAV TAYLKN SRK EHQRILARRQ TIEERKERLE SLNIQREKEE LEQREAELQK VRKAEEERLR QEAKEREKER ILQEHEQIKK KTVRERL EQ IKKTELGAKA FKDIDIEDLE ELDPDFIMAK QVEQLEKEKK ELQERLKNQE KKIDYFERAK RLEEIPLIKS AYEEQRIK D MDLWEQQEEE RITTMQLERE KALEHKNRMS RMLEDRDLFV MRLKAARQSV YEEKLKQFEE RLAEERHNRL EERKRQRKE ERRITYYREK EEEEQRRAEE QMLKEREERE RAERAKREEE LREYQERVKK LEEVERKKRQ RELEIEERER RREEERRLGE DPLSRRDSR WGDRDSEGTW RKGPEADSEW RRGPPEKEWR RGEGRDDERS HRRDEERPRR VGDDEDRESS LRPDDDRTLR R GMEDDRGP RRGPDEERFS RRGLEDERPS WRSTDDDRPP RRIGEEDRGS WRHAEDDRPP RRGLDDDRGS WRTADEDRGP RR MMDEDRG PRRGGADDER PSWRSADDDR GPRRGIDDDR GPRRGLDDDR GPWRNADDDR IPRRGADDDR GPWRNMDDDR MSR RADDDR ISRRGEDSRP GPWRPFVKPG GWREKEKARE ESWGPPRESR PPEEREWERE RDRDNPDREE NDKDPERERD RERD GDREE RFRRPRDEGG WRRGPAEEAS SWRDSSRRDD RDRDDRRRER DDRRDLRDRR DLRDDRDRRG PPLRSERDEV SSWRR ADDR KDDRAEERDP PRRVPPPVLS RDRDRDRDRD RDRDRERERE REREGEKEKT SWRAEKDRES LRRTKNETDE DGWTTV RR

UniProtKB: Eukaryotic translation initiation factor 3 subunit A

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Macromolecule #52: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 52 / Number of copies: 2 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.4 mg/mL
BufferpH: 7.4
Component:
ConcentrationFormulaName
10.0 mMHEPES-KOHHEPES Buffer
5.0 mMMg(OAc)2Magnesium Acetate
50.0 mMKOAcPotassium Acetate
10.0 mMNH4ClAmmonium Chloride
2.0 mMDTTDithiothreitol

Details: 10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec. / Details: glow-discharged at 2.5 mA for 20 s
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
Details: The sample was incubated on the grid for 30 s and then blotted with filter paper from both sides for 1.5 s with the blot force 5.
Detailslate-stage 48S initiation translation complex with eIF3 (LS48S-eIF3 IC) was dissolved in 20 microlitre buffer containing 10 mM HEPES-KOH pH 7.4, 5 mM Mg(OAc)2, 50 mM KOAc, 10 mM NH4Cl, 2 mM DTT and Roche cOmplete protease inhibitor cocktail

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Electron microscopy

MicroscopeFEI TALOS ARCTICA
TemperatureMin: 90.0 K / Max: 95.0 K
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-40 / Number grids imaged: 1 / Number real images: 9524 / Average exposure time: 6.0 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 30.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 59000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 4) / Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.9 Å / Resolution method: OTHER / Software - Name: cryoSPARC (ver. 4.5.1) / Number images used: 1000
Initial angle assignmentType: COMMON LINE
Final angle assignmentType: PROJECTION MATCHING
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChainDetails

8p09

source_name: PDB, initial_model_type: experimental model

6yam

source_name: PDB, initial_model_type: experimental model

6fyx

source_name: PDB, initial_model_type: experimental model

tgw1

chain_id: o, residue_range: 1-147, source_name: AlphaFold, initial_model_type: in silico modelEukaryotic translation initiation factor 5 (N-terminal domain)
DetailsThe atomic model of LS48S IC complex (PDB: 8P09) was initially rigid body fitted in density using ChimeraX and further model building was done for mRNA, eIF5 N-terminal domain, eIF2gamma, eIF3d and eIF3 octamer in ChimeraX. Densities for eIF5 N-terminal domain, eIF2gamma, eIF3d and eIF3 octamer were observed in the map and therefore were included in the model. The 3D structure of the eIF5-N terminal domain was generated by Alphafold2 (PDB: G1TGW1). It was then accommodated into the model by aligning structurally to its counterparts in the yeast atomic model of the py48S-eIF5N pre-initiation complex (PDB: 6FYX). IF3d and eIF3 octamer were taken from the atomic model of LS48S IC complex with eIF3 proteins (PDB: 6YAM) and placed into density by rigid-body fitting. The atomic models with and without eIF3 proteins were inspected visually and performed stereochemical, torsional, angle refinement and optimization by using ISOLDE v1.6. Figures featuring cryo-EM densities and atomic models were visualized by using ChimeraX.
RefinementProtocol: RIGID BODY FIT
Output model

PDB-9h74:
Late-stage 48S Initiation Complex with eIF3 (LS48S-eIF3 IC) guided by the trans-RNA

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