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データを開く
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基本情報
登録情報 | ![]() | ||||||||||||
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タイトル | 60S ribosomal subunit in complex with E3-UFM1 ligase and RQC machinery components NEMF and LTN1 (Composite map) | ||||||||||||
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![]() | 60S / UFMylation / ER / RQC / RIBOSOME | ||||||||||||
機能・相同性 | ![]() positive regulation of metallopeptidase activity / : / positive regulation of I-kappaB phosphorylation / alpha-aminoacyl-tRNA binding / UFM1 ligase activity / UFM1-modified protein reader activity / positive regulation of reticulophagy / regulation of phosphatase activity / apoptotic nuclear changes / definitive erythrocyte differentiation ...positive regulation of metallopeptidase activity / : / positive regulation of I-kappaB phosphorylation / alpha-aminoacyl-tRNA binding / UFM1 ligase activity / UFM1-modified protein reader activity / positive regulation of reticulophagy / regulation of phosphatase activity / apoptotic nuclear changes / definitive erythrocyte differentiation / CAT tailing / UFM1 transferase activity / negative regulation of protein serine/threonine kinase activity / positive regulation of protein localization to endoplasmic reticulum / protein K69-linked ufmylation / positive regulation of proteolysis involved in protein catabolic process / negative regulation of protein kinase activity by regulation of protein phosphorylation / protein ufmylation / positive regulation of plasma cell differentiation / RQC complex / negative regulation of IRE1-mediated unfolded protein response / regulation of proteasomal ubiquitin-dependent protein catabolic process / negative regulation of T cell mediated immune response to tumor cell / protein localization to endoplasmic reticulum / positive regulation of cell cycle G1/S phase transition / negative regulation of T cell activation / regulation of intracellular estrogen receptor signaling pathway / eukaryotic 80S initiation complex / negative regulation of protein neddylation / positive regulation of proteasomal protein catabolic process / regulation of G1 to G0 transition / axial mesoderm development / negative regulation of formation of translation preinitiation complex / mitotic G2/M transition checkpoint / ribosomal protein import into nucleus / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / 90S preribosome assembly / nuclear export / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / ribosome disassembly / 転移酵素; アシル基を移すもの; アミノアシル基を移すもの / regulation of canonical NF-kappaB signal transduction / mitogen-activated protein kinase binding / GAIT complex / A band / positive regulation of DNA damage response, signal transduction by p53 class mediator / TORC2 complex binding / alpha-beta T cell differentiation / reticulophagy / G1 to G0 transition / regulation of cyclin-dependent protein serine/threonine kinase activity / ribosome-associated ubiquitin-dependent protein catabolic process / cartilage development / regulation of neuron differentiation / middle ear morphogenesis / exit from mitosis / translation at presynapse / optic nerve development / cytoplasmic side of rough endoplasmic reticulum membrane / retinal ganglion cell axon guidance / negative regulation of ubiquitin protein ligase activity / negative regulation of protein import into nucleus / response to L-glutamate / homeostatic process / response to aldosterone / negative regulation of NF-kappaB transcription factor activity / macrophage chemotaxis / mitotic G2 DNA damage checkpoint signaling / negative regulation of PERK-mediated unfolded protein response / lung morphogenesis / male meiosis I / ribosomal large subunit binding / Protein hydroxylation / ubiquitin-like protein ligase binding / Peptide chain elongation / Selenocysteine synthesis / negative regulation of protein phosphorylation / positive regulation of signal transduction by p53 class mediator / Formation of a pool of free 40S subunits / ubiquitin ligase inhibitor activity / Eukaryotic Translation Termination / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / blastocyst development / negative regulation of ubiquitin-dependent protein catabolic process / cellular response to actinomycin D / Viral mRNA Translation / RHOA GTPase cycle / negative regulation of MAP kinase activity / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / protein localization to nucleus / GTP hydrolysis and joining of the 60S ribosomal subunit / hematopoietic stem cell differentiation / NF-kappaB binding / L13a-mediated translational silencing of Ceruloplasmin expression / ubiquitin-like ligase-substrate adaptor activity / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / protein-RNA complex assembly 類似検索 - 分子機能 | ||||||||||||
生物種 | ![]() | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.0 Å | ||||||||||||
![]() | Penchev I / Gumbin S / Becker T / Kopito R / Beckmann R | ||||||||||||
資金援助 | European Union, ![]() ![]()
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![]() | ![]() タイトル: UFMylation orchestrates spatiotemporal coordination of RQC at the ER. 著者: Ivan Penchev / Samantha Gumbin / Francesco Scavone / Otto Berninghausen / Thomas Becker / Ron Kopito / Roland Beckmann / ![]() ![]() 要旨: Degradation of arrest peptides from endoplasmic reticulum (ER) translocon-bound 60 ribosomal subunits via the ribosome-associated quality control (ER-RQC) pathway requires covalent modification of ...Degradation of arrest peptides from endoplasmic reticulum (ER) translocon-bound 60 ribosomal subunits via the ribosome-associated quality control (ER-RQC) pathway requires covalent modification of RPL26/uL24 on 60 ribosomal subunits with UFM1. However, the underlying mechanism that coordinates the UFMylation and RQC pathways remains elusive. Structural analysis of ER-RQC intermediates revealed concomitant binding and direct interaction of the UFMylation and RQC machineries on the 60. In the presence of an arrested peptidyl-transfer RNA, the RQC factor NEMF and the UFM1 E3 ligase (E3) form a direct interaction via the UFL1 subunit of E3, and UFL1 adopts a conformation distinct from that previously observed for posttermination 60. While this concomitant binding occurs on translocon-bound 60, LTN1 recruitment and arrest peptide degradation require UFMylation-dependent 60 dissociation from the translocon. These data reveal a mechanism by which the UFMylation cycle orchestrates ER-RQC. | ||||||||||||
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XML形式データ | ![]() | 8.8 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 9gy4MC C: 同じ文献を引用 ( M: このマップから作成された原子モデル |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 0.727 Å | ||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : 60S ribosomal subunit in complex with E3-UFM1 ligase and RQC mach...
+超分子 #1: 60S ribosomal subunit in complex with E3-UFM1 ligase and RQC mach...
+超分子 #3: UFM1 E3 ligase complex
+超分子 #4: RQC machinery
+超分子 #2: 60S ribosomal subunit
+分子 #1: 28S rRNA
+分子 #2: 5S rRNA
+分子 #3: 5.8S rRNA
+分子 #9: tRNA
+分子 #4: CDK5 regulatory subunit-associated protein 3
+分子 #5: DDRGK domain-containing protein 1
+分子 #6: Ubiquitin-fold modifier 1
+分子 #7: E3 UFM1-protein ligase 1
+分子 #8: E3 ubiquitin-protein ligase listerin
+分子 #10: 60S ribosomal protein L3
+分子 #11: 60S ribosomal protein L4
+分子 #12: 60S ribosomal protein L5
+分子 #13: Large ribosomal subunit protein eL6
+分子 #14: Large ribosomal subunit protein uL30
+分子 #15: 60S ribosomal protein L7a
+分子 #16: 60S ribosomal protein L9
+分子 #17: Ribosomal protein uL16-like
+分子 #18: 60S ribosomal protein L11
+分子 #19: 60S ribosomal protein L13
+分子 #20: 60S ribosomal protein L14
+分子 #21: 60S ribosomal protein L15
+分子 #22: 60S ribosomal protein L13a
+分子 #23: 60S ribosomal protein L17
+分子 #24: 60S ribosomal protein L18
+分子 #25: 60S ribosomal protein L19
+分子 #26: 60S ribosomal protein L18a
+分子 #27: 60S ribosomal protein L21
+分子 #28: 60S ribosomal protein L22
+分子 #29: 60S ribosomal protein L23
+分子 #30: 60S ribosomal protein L24
+分子 #31: 60S ribosomal protein L23a
+分子 #32: 60S ribosomal protein L26
+分子 #33: 60S ribosomal protein L27
+分子 #34: 60S ribosomal protein L27a
+分子 #35: 60S ribosomal protein L29
+分子 #36: 60S ribosomal protein L30
+分子 #37: 60S ribosomal protein L31
+分子 #38: 60S ribosomal protein L32
+分子 #39: 60S ribosomal protein L35a
+分子 #40: 60S ribosomal protein L34
+分子 #41: 60S ribosomal protein L35
+分子 #42: 60S ribosomal protein L36
+分子 #43: 60S ribosomal protein L37
+分子 #44: 60S ribosomal protein L38
+分子 #45: 60S ribosomal protein L39
+分子 #46: Ubiquitin-60S ribosomal protein L40
+分子 #47: 60S ribosomal protein L36a
+分子 #48: 60S ribosomal protein L37a
+分子 #49: 60S ribosomal protein L28
+分子 #50: 60S ribosomal protein L10a
+分子 #51: Ribosome quality control complex subunit NEMF
+分子 #52: 60S ribosomal protein L8
+分子 #53: 60S acidic ribosomal protein P0
+分子 #54: Large ribosomal subunit protein uL11
+分子 #55: Nascent chain
+分子 #56: MAGNESIUM ION
+分子 #57: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | TFS KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 40.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: OTHER / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |