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- EMDB-51555: Sub-tomogram average of Salmonella flagellar tip -

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Basic information

Entry
Database: EMDB / ID: EMD-51555
TitleSub-tomogram average of Salmonella flagellar tip
Map dataThe map of Salmonella flagellar tip
Sample
  • Organelle or cellular component: Salmonella flagellar tip
KeywordsFliD / FliC / Salmonella enterica / flagella / filament / cap / TRANSPORT PROTEIN
Biological speciesSalmonella enterica (bacteria)
Methodelectron tomography / cryo EM
AuthorsAl-Otaibi NS / Mann D / Bergeron JRC
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Biotechnology and Biological Sciences Research Council (BBSRC)BB/R009759/2 United Kingdom
CitationJournal: To Be Published
Title: Structure of the complete extracellular bacterial flagellum reveals mechanism for flagellin incorporation
Authors: Qin K / Einenkel R / Al-Otaibi NS / Schmidt J / Mann D / Drobnic T / Cohen EJ / Gonzalez-Rodriguez N / Harrowell J / Shmakova E / Beeby M / Erhardt M / Bergeron JRC
History
DepositionSep 16, 2024-
Header (metadata) releaseApr 9, 2025-
Map releaseApr 9, 2025-
UpdateApr 9, 2025-
Current statusApr 9, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51555.png

Downloads & links

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Map

FileDownload / File: emd_51555.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe map of Salmonella flagellar tip
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
3.63 Å/pix.
x 256 pix.
= 929.28 Å		3.63 Å/pix.
x 256 pix.
= 929.28 Å		3.63 Å/pix.
x 256 pix.
= 929.28 Å

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 3.63 Å
Density

Histogram

Histogram (log scale)
Minimum - Maximum-0.39587814 - 2.9243197
Average (Standard dev.)0.018605722 (±0.22437936)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 929.28 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Salmonella flagellar tip

EntireName: Salmonella flagellar tip
Components
  • Organelle or cellular component: Salmonella flagellar tip

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Supramolecule #1: Salmonella flagellar tip

SupramoleculeName: Salmonella flagellar tip / type: organelle_or_cellular_component / ID: 1 / Parent: 0
Source (natural)Organism: Salmonella enterica (bacteria)

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation statefilament

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE
SectioningOther: NO SECTIONING
Fiducial markerManufacturer: Nanoprobes / Diameter: 5 nm

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 2.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 8.0 µm / Nominal defocus min: 2.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionNumber images used: 68

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