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- EMDB-50944: Cryo electron tomogram of Streptomyces coelicolor - ScoDelta4242 ... -

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Basic information

Entry
Database: EMDB / ID: EMD-50944
TitleCryo electron tomogram of Streptomyces coelicolor - ScoDelta4242 complemented membrane protein CisA strain - Ghost cells
Map data
Sample
  • Complex: The contractile injection system in Streptomyces coelicolor
KeywordsContractile injection system / cryoET / STRUCTURAL PROTEIN
Biological speciesStreptomyces coelicolor A3(2) (bacteria)
Methodelectron tomography / cryo EM
AuthorsCasu B / Sallmen JW / Hass PE / Afanasyev P / Xu J / Schlimpert S / Pilhofer M
Funding support Switzerland, European Union, United Kingdom, 4 items
OrganizationGrant numberCountry
Swiss National Science Foundation31003A_179255 Switzerland
European Research Council (ERC)679209European Union
Biotechnology and Biological Sciences Research Council (BBSRC)BB/T015349/1 United Kingdom
Biotechnology and Biological Sciences Research Council (BBSRC)BB/X01097X/1 United Kingdom
CitationJournal: To Be Published
Title: Firing and cellular function of the Streptomyces coelicolor contractile injection system require the membrane protein CisA
Authors: Casu B / Sallmen JW / Hass PE / Afanasyev P / Xu J / Schlimpert S / Pilhofer M
History
DepositionJul 8, 2024-
Header (metadata) releaseJun 18, 2025-
Map releaseJun 18, 2025-
UpdateJun 18, 2025-
Current statusJun 18, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_50944.png

Downloads & links

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Map

FileDownload / File: emd_50944.map.gz / Format: CCP4 / Size: 340.3 MB / Type: IMAGE STORED AS SIGNED BYTE
Voxel sizeX=Y=Z: 10.74 Å
Density
Minimum - Maximum-128.0 - 127.0
Average (Standard dev.)-3.6228225 (±22.868728999999998)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin209-208121
Dimensions14401024242
Spacing10241440242
CellA: 10997.76 Å / B: 15465.6 Å / C: 2599.0798 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : The contractile injection system in Streptomyces coelicolor

EntireName: The contractile injection system in Streptomyces coelicolor
Components
  • Complex: The contractile injection system in Streptomyces coelicolor

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Supramolecule #1: The contractile injection system in Streptomyces coelicolor

SupramoleculeName: The contractile injection system in Streptomyces coelicolor
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Streptomyces coelicolor A3(2) (bacteria)

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Experimental details

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Structure determination

Methodcryo EM
Processingelectron tomography
Aggregation statecell

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE-PROPANE / Instrument: FEI VITROBOT MARK IV
SectioningOther: NO SECTIONING
Fiducial markerManufacturer: cytodiagnostics / Diameter: 10 nm

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number real images: 61 / Average electron dose: 2.1 e/Å2 / Details: 61 tilt series with around 130 accumulated dose.
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 8.0 µm / Nominal defocus min: 8.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionSoftware - Name: IMOD / Number images used: 61
CTF correctionType: NONE

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