ジャーナル: Dev Cell / 年: 2024 タイトル: Waves of regulated protein expression and phosphorylation rewire the proteome to drive gametogenesis in budding yeast. 著者: Rahel Wettstein / Jannik Hugener / Ludovic Gillet / Yi Hernández-Armenta / Adrian Henggeler / Jingwei Xu / Julian van Gerwen / Florian Wollweber / Meret Arter / Ruedi Aebersold / Pedro ...著者: Rahel Wettstein / Jannik Hugener / Ludovic Gillet / Yi Hernández-Armenta / Adrian Henggeler / Jingwei Xu / Julian van Gerwen / Florian Wollweber / Meret Arter / Ruedi Aebersold / Pedro Beltrao / Martin Pilhofer / Joao Matos / 要旨: Sexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a ...Sexually reproducing eukaryotes employ a developmentally regulated cell division program-meiosis-to generate haploid gametes from diploid germ cells. To understand how gametes arise, we generated a proteomic census encompassing the entire meiotic program of budding yeast. We found that concerted waves of protein expression and phosphorylation modify nearly all cellular pathways to support meiotic entry, meiotic progression, and gamete morphogenesis. Leveraging this comprehensive resource, we pinpointed dynamic changes in mitochondrial components and showed that phosphorylation of the FF-ATP synthase complex is required for efficient gametogenesis. Furthermore, using cryoET as an orthogonal approach to visualize mitochondria, we uncovered highly ordered filament arrays of Ald4, a conserved aldehyde dehydrogenase that is highly expressed and phosphorylated during meiosis. Notably, phosphorylation-resistant mutants failed to accumulate filaments, suggesting that phosphorylation regulates context-specific Ald4 polymerization. Overall, this proteomic census constitutes a broad resource to guide the exploration of the unique sequence of events underpinning gametogenesis.
集束イオンビーム - 装置: OTHER / 集束イオンビーム - イオン: OTHER / 集束イオンビーム - 電圧: 30 / 集束イオンビーム - 電流: 0.05 / 集束イオンビーム - 時間: 1500 / 集束イオンビーム - 温度: 123 K / 集束イオンビーム - Initial thickness: 4000 / 集束イオンビーム - 最終 厚さ: 200 集束イオンビーム - 詳細: The value given for _em_focused_ion_beam.instrument is Crossbeam 550 FIB-1567 SEM. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.
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電子顕微鏡法
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FEI TITAN KRIOS
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フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.96 e/Å2