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- EMDB-49237: Closed conformation of ArsA from L. ferriphilum in complex with M... -

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Basic information

Entry
Database: EMDB / ID: EMD-49237
TitleClosed conformation of ArsA from L. ferriphilum in complex with MgATP and arsenite at 1.5 minute time point
Map data
Sample
  • Complex: ArsA closed state in complex with MgATP and arsenite
    • Protein or peptide: Arsenite transporter ATPase-like protein,arsA
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ARSENIC
  • Ligand: water
KeywordsATPase / closed conformation / arsenite / arsenic / ATP / Intradimeric Walker A / HYDROLASE
Function / homology
Function and homology information


arsenite-transporting ATPase / ATPase-coupled arsenite transmembrane transporter activity / ATP hydrolysis activity / ATP binding
Similarity search - Function
Arsenical pump-driving ATPase / Arsenical pump ATPase, ArsA/GET3 / Anion-transporting ATPase-like domain / Anion-transporting ATPase / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
arsenite-transporting ATPase
Similarity search - Component
Biological speciesLeptospirillum ferriphilum ML-04 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsMahajan S / Rees DC / Clemons WM
Funding support United States, 1 items
OrganizationGrant numberCountry
Howard Hughes Medical Institute (HHMI) United States
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2025
Title: Nucleotide- and metalloid-driven conformational changes in the arsenite efflux ATPase ArsA
Authors: Mahajan S / Pall AE / Li YE / Stemmler TL / Rees DC / Clemons Jr WM
History
DepositionFeb 14, 2025-
Header (metadata) releaseAug 20, 2025-
Map releaseAug 20, 2025-
UpdateAug 20, 2025-
Current statusAug 20, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_49237.png

Downloads & links

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Map

FileDownload / File: emd_49237.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.65 Å/pix.
x 320 pix.
= 208. Å		0.65 Å/pix.
x 320 pix.
= 208. Å		0.65 Å/pix.
x 320 pix.
= 208. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.65 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.1
Minimum - Maximum-0.5865047 - 0.89973295
Average (Standard dev.)0.00009803251 (±0.01620097)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 208.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_49237_additional_1.map
Projections & Slices
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Half map: #2

Fileemd_49237_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_49237_half_map_2.map
Projections & Slices
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Slices (1/2)
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Histogram

Histogram (log scale)

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Sample components

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Entire : ArsA closed state in complex with MgATP and arsenite

EntireName: ArsA closed state in complex with MgATP and arsenite
Components
  • Complex: ArsA closed state in complex with MgATP and arsenite
    • Protein or peptide: Arsenite transporter ATPase-like protein,arsA
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: ARSENIC
  • Ligand: water

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Supramolecule #1: ArsA closed state in complex with MgATP and arsenite

SupramoleculeName: ArsA closed state in complex with MgATP and arsenite / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Details: ArsA incubated with 2 mM each of MgCl2, ATP and sodium arsenite for 1.5 min before vitrification
Source (natural)Organism: Leptospirillum ferriphilum ML-04 (bacteria)

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Macromolecule #1: Arsenite transporter ATPase-like protein,arsA

MacromoleculeName: Arsenite transporter ATPase-like protein,arsA / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Leptospirillum ferriphilum ML-04 (bacteria)
Molecular weightTheoretical: 63.972004 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MGMKFLQLPP RFMFFTGKGG VGKTSIACAT SIQLANAGKR VLLVSTDPAS NVGQVFGVDI GNRVTPIPAV PHLSALEIDP EAAASAYRE RLVGPVRGVL PDDVVKGIEE SLSGACTTEI AAFDEFTALL TNAVLTADYQ HIIFDTAPTG HTIRLLQLPG A WSGFLEAG ...String:
MGMKFLQLPP RFMFFTGKGG VGKTSIACAT SIQLANAGKR VLLVSTDPAS NVGQVFGVDI GNRVTPIPAV PHLSALEIDP EAAASAYRE RLVGPVRGVL PDDVVKGIEE SLSGACTTEI AAFDEFTALL TNAVLTADYQ HIIFDTAPTG HTIRLLQLPG A WSGFLEAG KGDASCLGPL AGLEKQRTQY KAAVEALADP LQTRLVLVAR AQQATLREVA RTHEELATIG IKQQHLVING IL PSAEAAN DPLAAAIHER EQTALKNIPA TLTSLPRDLV QLKPFNLVGL DALRQLLTDL PLQAHVAADA PIELDEPGMG DLV DGIEAD GHGLVMLMGK GGVGKTTLAA AIAVELAHRG LPVHLTTSDP AAHLTDTLEA SLDNLTVSRI DPHAETERYR QHVL ETKGA QLDAEGRALL EEDLHSPCTE EIAVFQAFSR IIREAGKKFV VMDTAPTGHT LLLLDATGAY HREVRRQMGN KGTHF TTPM MQLRDPNQTK VLVVTLAETT PVLEAAKLQA DLRRAGIEPW AWIINTSVAA ASAKSPLLRQ RAANELREIN AVANHH ADR YAVVPLLKEE PIGAERLRAL IHPQTHHHHH H

UniProtKB: arsenite-transporting ATPase

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Macromolecule #2: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 2 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #3: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 3 / Number of copies: 2 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #4: ARSENIC

MacromoleculeName: ARSENIC / type: ligand / ID: 4 / Number of copies: 1 / Formula: ARS
Molecular weightTheoretical: 74.922 Da
Chemical component information

ChemComp-ARS:
ARSENIC

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Macromolecule #5: water

MacromoleculeName: water / type: ligand / ID: 5 / Number of copies: 13 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration10 mg/mL
BufferpH: 7.5
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
DetailsArsA incubated with 2 mM each of MgCl2, ATP and sodium arsenite for 1.5 min before vitrification

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 70.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 130000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 124599
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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