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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Aminopeptidase N close state | |||||||||
![]() | Aminopeptidase N close state | |||||||||
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![]() | human / kidney / Aminopeptidase N / MEMBRANE PROTEIN / HYDROLASE | |||||||||
Function / homology | ![]() alanyl aminopeptidase activity / membrane alanyl aminopeptidase / peptide catabolic process / endoplasmic reticulum-Golgi intermediate compartment / metalloaminopeptidase activity / aminopeptidase activity / Metabolism of Angiotensinogen to Angiotensins / angiotensin maturation / peptide binding / secretory granule membrane ...alanyl aminopeptidase activity / membrane alanyl aminopeptidase / peptide catabolic process / endoplasmic reticulum-Golgi intermediate compartment / metalloaminopeptidase activity / aminopeptidase activity / Metabolism of Angiotensinogen to Angiotensins / angiotensin maturation / peptide binding / secretory granule membrane / metallopeptidase activity / signaling receptor activity / virus receptor activity / angiogenesis / cell differentiation / lysosomal membrane / external side of plasma membrane / Neutrophil degranulation / proteolysis / extracellular space / extracellular exosome / zinc ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.96 Å | |||||||||
![]() | Lyu M / Zhang Z / Tringides M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Aminopeptidase N close state Authors: Lyu M / Zhang Z | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 63.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.7 KB 15.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 10.5 KB | Display | ![]() |
Images | ![]() | 60.2 KB | ||
Filedesc metadata | ![]() | 5.9 KB | ||
Others | ![]() ![]() | 116.1 MB 116.1 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 19.2 KB | Display | |
Data in CIF | ![]() | 25 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9camMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||
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Annotation | Aminopeptidase N close state | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map B
File | emd_45396_half_map_1.map | ||||||||||||
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Annotation | Half Map B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map A
File | emd_45396_half_map_2.map | ||||||||||||
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Annotation | Half Map A | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Aminopeptidase N
Entire | Name: Aminopeptidase N |
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Components |
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-Supramolecule #1: Aminopeptidase N
Supramolecule | Name: Aminopeptidase N / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Aminopeptidase N
Macromolecule | Name: Aminopeptidase N / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: membrane alanyl aminopeptidase |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 109.641078 KDa |
Sequence | String: MAKGFYISKS LGILGILLGV AAVCTIIALS VVYSQEKNKN ANSSPVASTT PSASATTNPA SATTLDQSKA WNRYRLPNTL KPDSYRVTL RPYLTPNDRG LYVFKGSSTV RFTCKEATDV IIIHSKKLNY TLSQGHRVVL RGVGGSQPPD IDKTELVEPT E YLVVHLKG ...String: MAKGFYISKS LGILGILLGV AAVCTIIALS VVYSQEKNKN ANSSPVASTT PSASATTNPA SATTLDQSKA WNRYRLPNTL KPDSYRVTL RPYLTPNDRG LYVFKGSSTV RFTCKEATDV IIIHSKKLNY TLSQGHRVVL RGVGGSQPPD IDKTELVEPT E YLVVHLKG SLVKDSQYEM DSEFEGELAD DLAGFYRSEY MEGNVRKVVA TTQMQAADAR KSFPCFDEPA MKAEFNITLI HP KDLTALS NMLPKGPSTP LPEDPNWNVT EFHTTPKMST YLLAFIVSEF DYVEKQASNG VLIRIWARPS AIAAGHGDYA LNV TGPILN FFAGHYDTPY PLPKSDQIGL PDFNAGAMEN WGLVTYRENS LLFDPLSSSS SNKERVVTVI AHELAHQWFG NLVT IEWWN DLWLNEGFAS YVEYLGADYA EPTWNLKDLM VLNDVYRVMA VDALASSHPL STPASEINTP AQISELFDAI SYSKG ASVL RMLSSFLSED VFKQGLASYL HTFAYQNTIY LNLWDHLQEA VNNRSIQLPT TVRDIMNRWT LQMGFPVITV DTSTGT LSQ EHFLLDPDSN VTRPSEFNYV WIVPITSIRD GRQQQDYWLI DVRAQNDLFS TSGNEWVLLN LNVTGYYRVN YDEENWR KI QTQLQRDHSA IPVINRAQII NDAFNLASAH KVPVTLALNN TLFLIEERQY MPWEAALSSL SYFKLMFDRS EVYGPMKN Y LKKQVTPLFI HFRNNTNNWR EIPENLMDQY SEVNAISTAC SNGVPECEEM VSGLFKQWME NPNNNPIHPN LRSTVYCNA IAQGGEEEWD FAWEQFRNAT LVNEADKLRA ALACSKELWI LNRYLSYTLN PDLIRKQDAT STIISITNNV IGQGLVWDFV QSNWKKLFN DYGGGSFSFS NLIQAVTRRF STEYELQQLE QFKKDNEETG FGSGTRALEQ ALEKTKANIK WVKENKEVVL Q WFTENSK UniProtKB: Aminopeptidase N |
-Macromolecule #4: 2-acetamido-2-deoxy-beta-D-glucopyranose
Macromolecule | Name: 2-acetamido-2-deoxy-beta-D-glucopyranose / type: ligand / ID: 4 / Number of copies: 7 / Formula: NAG |
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Molecular weight | Theoretical: 221.208 Da |
Chemical component information | ![]() ChemComp-NAG: |
-Macromolecule #5: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 5 / Number of copies: 2 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 38.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 81000 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |