Entire : Wild-type human CFTR solubilized in digitonin and cholesterol-hem...
Entire
Name: Wild-type human CFTR solubilized in digitonin and cholesterol-hemisuccinate plus T2a nanobody in the presence of 2 mM MgATP
Components
Complex: Wild-type human CFTR solubilized in digitonin and cholesterol-hemisuccinate plus T2a nanobody in the presence of 2 mM MgATP
Organelle or cellular component: Wild type human Cystic Fibrosis Transmembrane Conductance Regulator (hCFTR)
Organelle or cellular component: T2a nanobody
Protein or peptide: T2a nanobody
Protein or peptide: Wild type human Cystic Fibrosis Transmembrane Conductance Regulator (hCFTR)
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Supramolecule #1: Wild-type human CFTR solubilized in digitonin and cholesterol-hem...
Supramolecule
Name: Wild-type human CFTR solubilized in digitonin and cholesterol-hemisuccinate plus T2a nanobody in the presence of 2 mM MgATP type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Molecular weight
Theoretical: 15.8 KDa
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Supramolecule #2: Wild type human Cystic Fibrosis Transmembrane Conductance Regulat...
Model: Quantifoil R0.6/1 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Support film - Film thickness: 50 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 25 sec. / Pretreatment - Atmosphere: OTHER / Pretreatment - Pressure: 0.026000000000000002 kPa Details: Gatan Solaris Plasma Cleaner 950, 15 watts cleaning power, O2/H2 at 27.5/6.4 sccm
Vitrification
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV
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Electron microscopy
Microscope
FEI TITAN KRIOS
Temperature
Min: 85.0 K / Max: 90.0 K
Specialist optics
Energy filter - Name: GIF Quantum ER / Energy filter - Slit width: 20 eV
Image recording
Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 11110 / Average exposure time: 2.5 sec. / Average electron dose: 58.06 e/Å2 Details: Movies comprised 50 frames collected in 2.5 seconds.
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Number classes used: 1 / Applied symmetry - Point group: C1 (asymmetric) / Algorithm: BACK PROJECTION / Resolution.type: BY AUTHOR / Resolution: 2.99 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.3.1) / Number images used: 102624
Initial angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.3.1)
Final angle assignment
Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.3.1)
Final 3D classification
Number classes: 16 / Avg.num./class: 29000 / Software - Name: cryoSPARC (ver. 4.3.1) Details: Final cycle of iterative heterorefinement against 11 protein volumes and 4 dummy volumes.
FSC plot (resolution estimation)
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