National Health and Medical Research Council (NHMRC, Australia)
APP1197376
オーストラリア
Australian Research Council (ARC)
DP230102150
オーストラリア
Australian Research Council (ARC)
LE200100045
オーストラリア
Australian Research Council (ARC)
LE120100090
オーストラリア
引用
ジャーナル: Sci Adv / 年: 2025 タイトル: High-affinity PQQ import is widespread in Gram-negative bacteria. 著者: Fabian Munder / Marcos Voutsinos / Klaus Hantke / Hari Venugopal / Rhys Grinter / 要旨: Pyrroloquinoline quinone (PQQ) is a soluble redox cofactor used by diverse bacteria. Many Gram-negative bacteria that encode PQQ-dependent enzymes do not produce it and instead obtain it from the ...Pyrroloquinoline quinone (PQQ) is a soluble redox cofactor used by diverse bacteria. Many Gram-negative bacteria that encode PQQ-dependent enzymes do not produce it and instead obtain it from the environment. To achieve this, uses the TonB-dependent transporter PqqU as a high-affinity PQQ importer. Here, we show that PqqU binds PQQ with high affinity and determine the high-resolution structure of the PqqU-PQQ complex, revealing that PqqU undergoes conformational changes in PQQ binding to capture the cofactor in an internal cavity. We show that these conformational changes preclude the binding of a bacteriophage, which targets PqqU as a cell surface receptor. Guided by the PqqU-PQQ structure, we identify amino acids essential for PQQ import and leverage this information to map the presence of PqqU across Gram-negative bacteria. This reveals that PqqU is encoded by Gram-negative bacteria from at least 22 phyla occupying diverse habitats, indicating that PQQ is an important cofactor for bacteria that adopt diverse lifestyles and metabolic strategies.