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- EMDB-45164: Structure of the Shigella flexneri bacteriophage Sf14 - tail helical -

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Basic information

Entry
Database: EMDB / ID: EMD-45164
TitleStructure of the Shigella flexneri bacteriophage Sf14 - tail helical
Map data
Sample
  • Virus: Shigella phage Sf14 (virus)
    • Protein or peptide: Putative structural protein
    • Protein or peptide: gp40
KeywordsSf14 / VIRUS
Function / homologyStructural protein ORF10, bacteriophage KPP10 / Protein of unknown function DUF3383 / Bacteriophage KPP10, Structural protein ORF10 / Protein of unknown function (DUF3383) / Uncharacterized protein / Putative structural protein
Function and homology information
Biological speciesShigella phage Sf14 (virus)
Methodhelical reconstruction / cryo EM / Resolution: 3.4 Å
AuthorsSubramanian S / Kerns HR / Braverman SG / Doore SM
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI170608 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)U24GM116789 United States
CitationJournal: To Be Published
Title: Structure of the Shigella flexneri bacteriophage Sf14 tail baseplate
Authors: Subramanian S / Kerns HR / Braverman SG / Doore SM
History
DepositionMay 31, 2024-
Header (metadata) releaseFeb 5, 2025-
Map releaseFeb 5, 2025-
UpdateFeb 5, 2025-
Current statusFeb 5, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_45164.png

Downloads & links

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Map

FileDownload / File: emd_45164.map.gz / Format: CCP4 / Size: 824 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.82 Å/pix.
x 600 pix.
= 489.6 Å		0.82 Å/pix.
x 600 pix.
= 489.6 Å		0.82 Å/pix.
x 600 pix.
= 489.6 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.816 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.28
Minimum - Maximum-0.63445324 - 1.5039212
Average (Standard dev.)0.001136184 (±0.07593158)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-299-299-299
Dimensions600600600
Spacing600600600
CellA=B=C: 489.59998 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_45164_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_45164_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_45164_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Shigella phage Sf14

EntireName: Shigella phage Sf14 (virus)
Components
  • Virus: Shigella phage Sf14 (virus)
    • Protein or peptide: Putative structural protein
    • Protein or peptide: gp40

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Supramolecule #1: Shigella phage Sf14

SupramoleculeName: Shigella phage Sf14 / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all / NCBI-ID: 2024304 / Sci species name: Shigella phage Sf14 / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: No / Virus empty: No
Host (natural)Organism: Shigella flexneri Y (bacteria)

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Macromolecule #1: Putative structural protein

MacromoleculeName: Putative structural protein / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Shigella phage Sf14 (virus)
Molecular weightTheoretical: 48.83477 KDa
Recombinant expressionOrganism: Shigella flexneri Y (bacteria)
SequenceString: MWNPIVNVDI TLNTAGTTRE GFGLPLFLAS TDNFEERIRG YTSLTEVAED FDESTAAYKA AKQLWSQTPK VTQLYIGRRT MQYTVSIPD TVAEGSEYSL TVAIGGGVSQ PFQYTAKEND TALIVLNEFK SQIEASPTIK DGVNASVTGT GASATMIITK A GDNDFVKV ...String:
MWNPIVNVDI TLNTAGTTRE GFGLPLFLAS TDNFEERIRG YTSLTEVAED FDESTAAYKA AKQLWSQTPK VTQLYIGRRT MQYTVSIPD TVAEGSEYSL TVAIGGGVSQ PFQYTAKEND TALIVLNEFK SQIEASPTIK DGVNASVTGT GASATMIITK A GDNDFVKV TSITPTTSIA ATTADTASAA LASIETYSTD WYFISAEDRT QQFVLAMASE IQARKKIFFT ANADVKALQG TD LTSATDV PAQLAKSKYT RTVCLWHHTA EFDYPEMAYI AYGAPYDAGS IAWGNAQLTG VAASLQPANQ RPLISIQKSA LDT RSCNFI DLDGGVPVVR RGITSGGEWI DIVRGVDWLE SDLKTSLRDL LINQKGGKIT YDDTGITRIR QVIETSLQRA VNRK FLSTY TVTVPKASQV ALADKKARIL KDITFHGILA GAILDVDLKG TVAYE

UniProtKB: Putative structural protein

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Macromolecule #2: gp40

MacromoleculeName: gp40 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Shigella phage Sf14 (virus)
Molecular weightTheoretical: 16.204168 KDa
Recombinant expressionOrganism: Shigella flexneri Y (bacteria)
SequenceString:
MAMYQQYSPK DVVCSWNGIA IEGFAPDSFL RLQRTSPLVT PVVGAGGQVA LTRNADKTGT IEIELMQTSL SNQMLSAIQA KQDDMELEE DISSNFVIYD PSGSVLATGI NAWLQELPQI ELGRDQNSKT WIFGCEKLDY TSTIPASSV

UniProtKB: Uncharacterized protein

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Experimental details

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Structure determination

Methodcryo EM
Processinghelical reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Component:
ConcentrationFormulaName
10.0 mMTristris(hydroxymethyl)aminomethane
10.0 mMMgCl2magnesium chloride
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 33.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Helical parameters - Δz: 37.062 Å
Applied symmetry - Helical parameters - Δ&Phi: 25.75 °
Applied symmetry - Helical parameters - Axial symmetry: C6 (6 fold cyclic)
Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 23422
Startup modelType of model: NONE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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