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- EMDB-44559: Apo form Mre11-Rad50 complex -

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Basic information

Entry
Database: EMDB / ID: EMD-44559
TitleApo form Mre11-Rad50 complex
Map dataSharpen Map
Sample
  • Complex: Apo form yeast Mre11-Rad50 complex
    • Protein or peptide: DNA repair protein RAD50
    • Protein or peptide: Double-strand break repair protein MRE11
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MANGANESE (II) ION
KeywordsMRN / Mre11 / Rad50 / Nbs1 / DNA binding / DNA damage / DNA repair / DNA BINDING PROTEIN
Function / homology
Function and homology information


DNA double-strand break processing involved in repair via synthesis-dependent strand annealing / nucleoside monophosphate phosphorylation / Cytosolic sensors of pathogen-associated DNA / meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / mitochondrial double-strand break repair via homologous recombination / DNA Damage/Telomere Stress Induced Senescence / Sensing of DNA Double Strand Breaks / Mre11 complex / meiotic DNA double-strand break formation / ascospore formation ...DNA double-strand break processing involved in repair via synthesis-dependent strand annealing / nucleoside monophosphate phosphorylation / Cytosolic sensors of pathogen-associated DNA / meiotic DNA double-strand break formation involved in reciprocal meiotic recombination / mitochondrial double-strand break repair via homologous recombination / DNA Damage/Telomere Stress Induced Senescence / Sensing of DNA Double Strand Breaks / Mre11 complex / meiotic DNA double-strand break formation / ascospore formation / R-loop processing / Hydrolases; Acting on acid anhydrides / chromosome organization involved in meiotic cell cycle / single-stranded DNA endodeoxyribonuclease activity / homologous chromosome pairing at meiosis / AMP kinase activity / double-stranded telomeric DNA binding / maintenance of DNA trinucleotide repeats / G-quadruplex DNA binding / 3'-5'-DNA exonuclease activity / DNA double-strand break processing / single-stranded telomeric DNA binding / telomere maintenance via recombination / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / double-strand break repair via break-induced replication / reciprocal meiotic recombination / mitotic intra-S DNA damage checkpoint signaling / telomeric DNA binding / mitotic G2 DNA damage checkpoint signaling / telomere maintenance via telomerase / 3'-5' exonuclease activity / telomere maintenance / condensed nuclear chromosome / DNA endonuclease activity / meiotic cell cycle / double-strand break repair via homologous recombination / base-excision repair / double-strand break repair via nonhomologous end joining / site of double-strand break / double-strand break repair / manganese ion binding / double-stranded DNA binding / endonuclease activity / molecular adaptor activity / Hydrolases; Acting on ester bonds / chromosome, telomeric region / DNA repair / regulation of transcription by RNA polymerase II / ATP hydrolysis activity / mitochondrion / nucleoplasm / ATP binding / metal ion binding / nucleus
Similarity search - Function
DNA repair protein Rad50, eukaryotes / DNA double-strand break repair protein Mre11 / Mre11, DNA-binding / Mre11, capping domain / Mre11 DNA-binding presumed domain / Mre11 DNA-binding presumed domain / Rad50 zinc hook motif / RAD50, zinc hook / Rad50 zinc-hook domain profile. / Mre11 nuclease, N-terminal metallophosphatase domain ...DNA repair protein Rad50, eukaryotes / DNA double-strand break repair protein Mre11 / Mre11, DNA-binding / Mre11, capping domain / Mre11 DNA-binding presumed domain / Mre11 DNA-binding presumed domain / Rad50 zinc hook motif / RAD50, zinc hook / Rad50 zinc-hook domain profile. / Mre11 nuclease, N-terminal metallophosphatase domain / SbcC/RAD50-like, Walker B motif / Rad50/SbcC-type AAA domain / AAA domain / Calcineurin-like phosphoesterase domain, ApaH type / Calcineurin-like phosphoesterase / Metallo-dependent phosphatase-like / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
DNA repair protein RAD50 / Double-strand break repair protein MRE11
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsYu Y / Patel DJ
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM136278 United States
CitationJournal: To Be Published
Title: cryo EM structure of Apo form Mre11-Rad50 complex
Authors: Yu Y / Patel DJ
History
DepositionApr 22, 2024-
Header (metadata) releaseAug 6, 2025-
Map releaseAug 6, 2025-
UpdateAug 6, 2025-
Current statusAug 6, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_44559.png

Downloads & links

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Map

FileDownload / File: emd_44559.map.gz / Format: CCP4 / Size: 83.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpen Map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 280 pix.
= 303.24 Å		1.08 Å/pix.
x 280 pix.
= 303.24 Å		1.08 Å/pix.
x 280 pix.
= 303.24 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.083 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.25
Minimum - Maximum-1.4634956 - 2.5473688
Average (Standard dev.)-0.0004075265 (±0.045769263)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions280280280
Spacing280280280
CellA=B=C: 303.24 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Half Map A

Fileemd_44559_half_map_1.map
AnnotationHalf Map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half Map B

Fileemd_44559_half_map_2.map
AnnotationHalf Map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Apo form yeast Mre11-Rad50 complex

EntireName: Apo form yeast Mre11-Rad50 complex
Components
  • Complex: Apo form yeast Mre11-Rad50 complex
    • Protein or peptide: DNA repair protein RAD50
    • Protein or peptide: Double-strand break repair protein MRE11
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: MANGANESE (II) ION

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Supramolecule #1: Apo form yeast Mre11-Rad50 complex

SupramoleculeName: Apo form yeast Mre11-Rad50 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 460 KDa

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Macromolecule #1: DNA repair protein RAD50

MacromoleculeName: DNA repair protein RAD50 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO / EC number: Hydrolases; Acting on acid anhydrides
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 152.797688 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA TTGDLPPNSK GGVFIHDPKI TGEKDIRAQV KLAFTSANG LNMIVTRNIQ LLMKKTTTTF KTLEGQLVAI NNSGDRSTLS TRSLELDAQV PLYLGVPKAI LEYVIFCHQE D SLWPLSEP ...String:
MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA TTGDLPPNSK GGVFIHDPKI TGEKDIRAQV KLAFTSANG LNMIVTRNIQ LLMKKTTTTF KTLEGQLVAI NNSGDRSTLS TRSLELDAQV PLYLGVPKAI LEYVIFCHQE D SLWPLSEP SNLKKKFDEI FQAMKFTKAL DNLKSIKKDM SVDIKLLKQS VEHLKLDKDR SKAMKLNIHQ LQTKIDQYNE EV SEIESQL NEITEKSDKL FKSNQDFQKI LSKVENLKNT KLSISDQVKR LSNSIDILDL SKPDLQNLLA NFSKVLMDKN NQL RDLETD ISSLKDRQSS LQSLSNSLIR RQGELEAGKE TYEKNRNHLS SLKEAFQHKF QGLSNIENSD MAQVNHEMSQ FKAF ISQDL TDTIDQFAKD IQLKETNLSD LIKSITVDSQ NLEYNKKDRS KLIHDSEELA EKLKSFKSLS TQDSLNHELE NLKTY KEKL QSWESENIIP KLNQKIEEKN NEMIILENQI EKFQDRIMKT NQQADLYAKL GLIKKSINTK LDELQKITEK LQNDSR IRQ VFPLTQEFQR ADLEMDFQKL FINMQKNIAI NNKKMHELDR RYTNALYNLN TIEKDLQDNQ KSKEKVIQLL SENLPED CT IDEYNDVLEE TELSYKTALE NLKMHQTTLE FNRKALEIAE RDSCCYLCSR KFENESFKSK LLQELKTKTD ANFEKTLK D TVQNEKEYLH SLRLLEKHII TLNSINEKID NSQKCLEKAK EETKTSKSKL DELEVDSTKL KDEKELAESE IRPLIEKFT YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ RKMNDSLREL RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSL TQKQNIDDSI RSKRENINDI DSRVKELEAR IISLKNKKDE AQSVLDKVKN ERDIQVRNKQ KTVADINRLI D RFQTIYNE VVDFEAKGFD ELQTTIKELE LNKAQMLELK EQLDLKSNEV NEEKRKLADS NNEEKNLKQN LELIELKSQL QH IESEISR LDVQNAEAER DKYQEESLRL RTRFEKLSSE NAGKLGEMKQ LQNQIDSLTH QLRTDYKDIE KNYHKEWVEL QTR SFVTDD IDVYSKALDS AIMKYHGLKM QDINRIIDEL WKRTYSGTDI DTIKIRSDEV SSTVKGKSYN YRVVMYKQDV ELDM RGRCS AGQKVLASII IRLALSETFG ANCGVIALDQ PTTNLDEENI ESLAKSLHNI INMRRHQKNF QLIVITHDEK FLGHM NAAA FTDHFFKVKR DDRQKSQIEW VDINRVTY

UniProtKB: DNA repair protein RAD50

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Macromolecule #2: Double-strand break repair protein MRE11

MacromoleculeName: Double-strand break repair protein MRE11 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Molecular weightTheoretical: 79.607297 KDa
Recombinant expressionOrganism: Saccharomyces cerevisiae (brewer's yeast)
SequenceString: MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELEL LSDPSQVFHY DEFTNVNYED PNFNISIPVF GISGNHDDAS GDSLLCPMDI LHATGLINHF GKVIESDKIK V VPLLFQKG ...String:
MDYPDPDTIR ILITTDNHVG YNENDPITGD DSWKTFHEVM MLAKNNNVDM VVQSGDLFHV NKPSKKSLYQ VLKTLRLCCM GDKPCELEL LSDPSQVFHY DEFTNVNYED PNFNISIPVF GISGNHDDAS GDSLLCPMDI LHATGLINHF GKVIESDKIK V VPLLFQKG STKLALYGLA AVRDERLFRT FKDGGVTFEV PTMREGEWFN LMCVHQNHTG HTNTAFLPEQ FLPDFLDMVI WG HEHECIP NLVHNPIKNF DVLQPGSSVA TSLCEAEAQP KYVFILDIKY GEAPKMTPIP LETIRTFKMK SISLQDVPHL RPH DKDATS KYLIEQVEEM IRDANEETKQ KLADDGEGDM VAELPKPLIR LRVDYSAPSN TQSPIDYQVE NPRRFSNRFV GRVA NGNNV VQFYKKRSPV TRSKKSGING TSISDRDVEK LFSESGGELE VQTLVNDLLN KMQLSLLPEV GLNEAVKKFV DKDEK TALK EFISHEISNE VGILSTNEEF LRTDDAEEMK ALIKQVKRAN SVRPTPPKEN DETNFAFNGN GLDSFRSSNR EVRTGS PDI TQSHVDNESR ITHISQAESS KPTSKPKRVR TATKKKIPAF SDSTVISDAE NELGDNNDAQ DDVDIDENDI IMVSTDE ED ASYGLLNGRK TKTKTRPAAS TKTASRRGKG RASRTPKTDI LGSLLAKKRK YDYKDDDDKH HHHH

UniProtKB: Double-strand break repair protein MRE11

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Macromolecule #3: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 2 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #4: MANGANESE (II) ION

MacromoleculeName: MANGANESE (II) ION / type: ligand / ID: 4 / Number of copies: 4 / Formula: MN
Molecular weightTheoretical: 54.938 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
Details: 50 mM Hepes, pH 7.5, 200 mM NaCl, 2 mM DTT, 2% Glycerol, 0.025% (w/v) CHAPSO (3-([3-Cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate)
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 51.22 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 232521
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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