+データを開く
-基本情報
登録情報 | データベース: EMDB / ID: EMD-4427 | ||||||||||||
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タイトル | The structure of a di-ribosome (disome) as a unit for RQC and NGD quality control pathways recognition. | ||||||||||||
マップデータ | |||||||||||||
試料 |
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キーワード | ribosome / disome / di-ribosome / stalling / TRANSLATION | ||||||||||||
機能・相同性 | 機能・相同性情報 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / RMTs methylate histone arginines / positive regulation of translational fidelity / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process ...maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, LSU-rRNA,5S) / negative regulation of glucose mediated signaling pathway / negative regulation of translational frameshifting / Negative regulators of DDX58/IFIH1 signaling / RMTs methylate histone arginines / positive regulation of translational fidelity / Protein methylation / mTORC1-mediated signalling / Protein hydroxylation / ribosome-associated ubiquitin-dependent protein catabolic process / GDP-dissociation inhibitor activity / nonfunctional rRNA decay / hexon binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / pre-mRNA 5'-splice site binding / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / preribosome, small subunit precursor / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal scanning and start codon recognition / response to cycloheximide / Major pathway of rRNA processing in the nucleolus and cytosol / mRNA destabilization / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / negative regulation of mRNA splicing, via spliceosome / preribosome, large subunit precursor / regulation of amino acid metabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / G-protein alpha-subunit binding / positive regulation of protein kinase activity / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / regulation of translational fidelity / Ub-specific processing proteases / protein-RNA complex assembly / ribosomal subunit export from nucleus / ribosomal small subunit export from nucleus / translation regulator activity / translational termination / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / DNA-(apurinic or apyrimidinic site) endonuclease activity / maturation of LSU-rRNA / cellular response to amino acid starvation / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / ribosome assembly / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / translational initiation / macroautophagy / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / cytoplasmic stress granule / protein tag activity / rRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosome biogenesis / viral capsid / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / positive regulation of protein phosphorylation / G protein-coupled receptor signaling pathway / translation / negative regulation of gene expression / response to antibiotic / mRNA binding / ubiquitin protein ligase binding / host cell nucleus / nucleolus / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / nucleus / metal ion binding / cytosol / cytoplasm 類似検索 - 分子機能 | ||||||||||||
生物種 | Saccharomyces cerevisiae (パン酵母) | ||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.3 Å | ||||||||||||
データ登録者 | Tesina P / Cheng J | ||||||||||||
資金援助 | ドイツ, 日本, 3件
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引用 | ジャーナル: EMBO J / 年: 2019 タイトル: Collided ribosomes form a unique structural interface to induce Hel2-driven quality control pathways. 著者: Ken Ikeuchi / Petr Tesina / Yoshitaka Matsuo / Takato Sugiyama / Jingdong Cheng / Yasushi Saeki / Keiji Tanaka / Thomas Becker / Roland Beckmann / Toshifumi Inada / 要旨: Ribosome stalling triggers quality control pathways targeting the mRNA (NGD: no-go decay) and the nascent polypeptide (RQC: ribosome-associated quality control). RQC requires Hel2-dependent uS10 ...Ribosome stalling triggers quality control pathways targeting the mRNA (NGD: no-go decay) and the nascent polypeptide (RQC: ribosome-associated quality control). RQC requires Hel2-dependent uS10 ubiquitination and the RQT complex in yeast. Here, we report that Hel2-dependent uS10 ubiquitination and Slh1/Rqt2 are crucial for RQC and NGD induction within a di-ribosome (disome) unit, which consists of the leading stalled ribosome and the following colliding ribosome. Hel2 preferentially ubiquitinated a disome over a monosome on a quality control inducing reporter mRNA in an translation reaction. Cryo-EM analysis of the disome unit revealed a distinct structural arrangement suitable for recognition and modification by Hel2. The absence of the RQT complex or uS10 ubiquitination resulted in the elimination of NGD within the disome unit. Instead, we observed Hel2-mediated cleavages upstream of the disome, governed by initial Not4-mediated monoubiquitination of eS7 and followed by Hel2-mediated K63-linked polyubiquitination. We propose that Hel2-mediated ribosome ubiquitination is required both for canonical NGD (NGD) and RQC coupled to the disome and that RQC-uncoupled NGD outside the disome (NGD) can occur in a Not4-dependent manner. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | EMマップ: SurfViewMolmilJmol/JSmol |
添付画像 |
-ダウンロードとリンク
-EMDBアーカイブ
マップデータ | emd_4427.map.gz | 260.1 MB | EMDBマップデータ形式 | |
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ヘッダ (付随情報) | emd-4427-v30.xml emd-4427.xml | 96.4 KB 96.4 KB | 表示 表示 | EMDBヘッダ |
FSC (解像度算出) | emd_4427_fsc.xml | 17.9 KB | 表示 | FSCデータファイル |
画像 | emd_4427.png | 52.5 KB | ||
Filedesc metadata | emd-4427.cif.gz | 18.5 KB | ||
アーカイブディレクトリ | http://ftp.pdbj.org/pub/emdb/structures/EMD-4427 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-4427 | HTTPS FTP |
-検証レポート
文書・要旨 | emd_4427_validation.pdf.gz | 461.2 KB | 表示 | EMDB検証レポート |
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文書・詳細版 | emd_4427_full_validation.pdf.gz | 460.8 KB | 表示 | |
XML形式データ | emd_4427_validation.xml.gz | 16.2 KB | 表示 | |
CIF形式データ | emd_4427_validation.cif.gz | 22.5 KB | 表示 | |
アーカイブディレクトリ | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4427 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-4427 | HTTPS FTP |
-関連構造データ
-リンク
EMDBのページ | EMDB (EBI/PDBe) / EMDataResource |
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「今月の分子」の関連する項目 |
-マップ
ファイル | ダウンロード / ファイル: emd_4427.map.gz / 形式: CCP4 / 大きさ: 494.2 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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投影像・断面図 | 画像のコントロール
画像は Spider により作成 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 1.4996 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
-試料の構成要素
+全体 : Cryo-EM structure of a native stalled di-ribosome (disome) comple...
+超分子 #1: Cryo-EM structure of a native stalled di-ribosome (disome) comple...
+分子 #1: 25S ribosomal RNA
+分子 #2: 5S ribosomal RNA
+分子 #3: 5.8S ribosomal RNA
+分子 #18: 18S ribosomal RNA
+分子 #80: P-site tRNA
+分子 #81: E-site tRNA
+分子 #82: A/P hybrid tRNA
+分子 #83: P/E hybrid tRNA
+分子 #84: mRNA
+分子 #4: 60S ribosomal protein L2-A
+分子 #5: 60S ribosomal protein L3
+分子 #6: 60S ribosomal protein L4-A
+分子 #7: 60S ribosomal protein L5
+分子 #8: 60S ribosomal protein L6-A
+分子 #9: 60S ribosomal protein L7-A
+分子 #10: 60S ribosomal protein L8-A
+分子 #11: 60S ribosomal protein L9-A
+分子 #12: 60S ribosomal protein L10
+分子 #13: 60S ribosomal protein L11-B
+分子 #14: 60S ribosomal protein L13-A
+分子 #15: 60S ribosomal protein L14-A
+分子 #16: 60S ribosomal protein L15-A
+分子 #17: 60S ribosomal protein L16-A
+分子 #19: 40S ribosomal protein S0-A
+分子 #20: 40S ribosomal protein S1-A
+分子 #21: 40S ribosomal protein S2
+分子 #22: 40S ribosomal protein S3
+分子 #23: 40S ribosomal protein S4-A
+分子 #24: 40S ribosomal protein S5
+分子 #25: 40S ribosomal protein S6-A
+分子 #26: 40S ribosomal protein S7-A
+分子 #27: 40S ribosomal protein S8-A
+分子 #28: 40S ribosomal protein S9-A
+分子 #29: 40S ribosomal protein S10-A
+分子 #30: 40S ribosomal protein S11-A
+分子 #31: 40S ribosomal protein S12
+分子 #32: 40S ribosomal protein S13
+分子 #33: 40S ribosomal protein S14-B
+分子 #34: 40S ribosomal protein S15
+分子 #35: 40S ribosomal protein S16-A
+分子 #36: 40S ribosomal protein S17-A
+分子 #37: 40S ribosomal protein S18-A
+分子 #38: 40S ribosomal protein S19-A
+分子 #39: 40S ribosomal protein S20
+分子 #40: 40S ribosomal protein S21-A
+分子 #41: 40S ribosomal protein S22-A
+分子 #42: 40S ribosomal protein S23-A
+分子 #43: 40S ribosomal protein S24-A
+分子 #44: 40S ribosomal protein S25-A
+分子 #45: 40S ribosomal protein S26-B
+分子 #46: 40S ribosomal protein S27-A
+分子 #47: 40S ribosomal protein S28-A
+分子 #48: 40S ribosomal protein S29-A
+分子 #49: 40S ribosomal protein S30-A
+分子 #50: Ubiquitin-40S ribosomal protein S31
+分子 #51: Guanine nucleotide-binding protein subunit beta-like protein
+分子 #52: 60S ribosomal protein L17-A
+分子 #53: 60S ribosomal protein L18-A
+分子 #54: 60S ribosomal protein L19-A
+分子 #55: 60S ribosomal protein L20-A
+分子 #56: 60S ribosomal protein L21-A
+分子 #57: 60S ribosomal protein L22-A
+分子 #58: 60S ribosomal protein L23-A
+分子 #59: 60S ribosomal protein L24-A
+分子 #60: 60S ribosomal protein L25
+分子 #61: 60S ribosomal protein L26-A
+分子 #62: 60S ribosomal protein L27-A
+分子 #63: 60S ribosomal protein L28
+分子 #64: 60S ribosomal protein L29
+分子 #65: 60S ribosomal protein L30
+分子 #66: 60S ribosomal protein L31-A
+分子 #67: 60S ribosomal protein L32
+分子 #68: 60S ribosomal protein L33-A
+分子 #69: 60S ribosomal protein L34-A
+分子 #70: 60S ribosomal protein L35-A
+分子 #71: 60S ribosomal protein L36-A
+分子 #72: 60S ribosomal protein L37-A
+分子 #73: 60S ribosomal protein L38
+分子 #74: 60S ribosomal protein L39
+分子 #75: Ubiquitin-60S ribosomal protein L40
+分子 #76: 60S ribosomal protein L41-B
+分子 #77: 60S ribosomal protein L42-A
+分子 #78: 60S ribosomal protein L43-A
+分子 #79: 60S acidic ribosomal protein P0
+分子 #85: ZINC ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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解析 | 単粒子再構成法 |
試料の集合状態 | particle |
-試料調製
緩衝液 | pH: 7.2 |
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凍結 | 凍結剤: ETHANE |
-電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON II (4k x 4k) 平均電子線量: 2.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |