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- EMDB-43960: Tail-Baseplate of P1 bacteriophage -

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Open data


ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-43960
TitleTail-Baseplate of P1 bacteriophage
Map datamain baseplate map
Sample
  • Virus: Escherichia phage P1 (virus)
KeywordsPhage Baseplate / VIRUS
Function / homologyGp26 / Gp22 / BplB / Tub / BplA / Gp16 / PmgG / Pep44b
Function and homology information
Biological speciesEscherichia phage P1 (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 5.87 Å
AuthorsSen A
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: To Be Published
Title: Map of Tail-Baseplate of P1 bacteriophage
Authors: Sen A
History
DepositionMar 6, 2024-
Header (metadata) releaseSep 10, 2025-
Map releaseSep 10, 2025-
UpdateSep 10, 2025-
Current statusSep 10, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

Downloads & links

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Map

FileDownload / File: emd_43960.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationmain baseplate map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.39 Å/pix.
x 512 pix.
= 711.117 Å
1.39 Å/pix.
x 512 pix.
= 711.117 Å
1.39 Å/pix.
x 512 pix.
= 711.117 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.3889 Å
Density
Contour LevelBy AUTHOR: 0.22
Minimum - Maximum-0.3620384 - 0.65931654
Average (Standard dev.)-0.0000863025 (±0.039484117)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 711.1168 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: baseplate map half A

Fileemd_43960_half_map_1.map
Annotationbaseplate map half A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Half map: baseplate map half B

Fileemd_43960_half_map_2.map
Annotationbaseplate map half B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

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Sample components

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Entire : Escherichia phage P1

EntireName: Escherichia phage P1 (virus)
Components
  • Virus: Escherichia phage P1 (virus)

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Supramolecule #1: Escherichia phage P1

SupramoleculeName: Escherichia phage P1 / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 2886926 / Sci species name: Escherichia phage P1 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS GLACIOS
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 30.0 e/Å2
Electron beamAcceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER

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Image processing

Particle selectionNumber selected: 68526
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionApplied symmetry - Point group: C6 (6 fold cyclic) / Resolution.type: BY AUTHOR / Resolution: 5.87 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 12800
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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