Journal: Elife / Year: 2018 Title: Cryo-EM structure of respiratory complex I at work. Authors: Kristian Parey / Ulrich Brandt / Hao Xie / Deryck J Mills / Karin Siegmund / Janet Vonck / Werner Kühlbrandt / Volker Zickermann / Abstract: Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 ...Mitochondrial complex I has a key role in cellular energy metabolism, generating a major portion of the proton motive force that drives aerobic ATP synthesis. The hydrophilic arm of the L-shaped ~1 MDa membrane protein complex transfers electrons from NADH to ubiquinone, providing the energy to drive proton pumping at distant sites in the membrane arm. The critical steps of energy conversion are associated with the redox chemistry of ubiquinone. We report the cryo-EM structure of complete mitochondrial complex I from the aerobic yeast both in the deactive form and after capturing the enzyme during steady-state activity. The site of ubiquinone binding observed during turnover supports a two-state stabilization change mechanism for complex I.
History
Deposition
Apr 19, 2018
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Header (metadata) release
May 23, 2018
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Map release
Oct 10, 2018
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Update
Nov 6, 2019
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Current status
Nov 6, 2019
Processing site: PDBe / Status: Released
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Structure visualization
Movie
Surface view with section colored by density value
Cryogen name: ETHANE / Chamber humidity: 70 % / Chamber temperature: 291 K / Instrument: FEI VITROBOT MARK IV
Details
4 uM of complex I incubated with 400 uM DBQ was mixed in the ratio 1:1 with 2 uM bo3-type ubiquinol oxidase, TMV (0.13 mg/ml)and 4 mM NADH
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Electron microscopy
Microscope
FEI TITAN KRIOS
Image recording
Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: COUNTING / Number grids imaged: 2 / Number real images: 5964 / Average exposure time: 81.0 sec. / Average electron dose: 35.7 e/Å2
Electron beam
Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
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