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- EMDB-43549: E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P ... -

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Open data

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Basic information

Entry

Database: EMDB / ID: EMD-43549

Title

E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon

Map data

E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon

Sample

Complex: E.coli 70S complex with mRNA and e*/E-tRNA
- RNA: x 4 types
- Protein or peptide: x 49 types
RNA: x 1 types
Ligand: x 3 types

Keywords

70S ribosome / tRNA / frameshift / RIBOSOME

Function / homology

Function and homology information

negative regulation of cytoplasmic translational initiation / DnaA-L2 complex / negative regulation of translational initiation / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / ribosome assembly / cytosolic ribosome assembly / transcription antitermination / translational initiation ...
Similarity search - Function

Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. ...Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein L5, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein S4, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / 30S ribosomal protein S17 / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S5, bacterial-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L20 signature. / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L35 / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal L28 family / Ribosomal protein L33 superfamily / Ribosomal protein S9, bacterial/plastid / Ribosomal protein L16 / Ribosomal protein L28/L24 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L30, bacterial-type / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / L28p-like / Ribosomal protein S15, bacterial-type / : / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein L27 / Ribosomal L27 protein
Similarity search - Domain/homology

Large ribosomal subunit protein uL15 / Small ribosomal subunit protein bS18 / Large ribosomal subunit protein bL36 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL24 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein uL4 ...
Similarity search - Component

Biological species

Escherichia coli (E. coli)

Method

single particle reconstruction / cryo EM / Resolution: 3.4 Å

Authors

Kimbrough EM / Dunham CM / Nguyen HA

Funding support

United States, 3 items

Organization	Grant number	Country
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R01GM093278	United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)	R35GM34931	United States
National Science Foundation (NSF, United States)	1808771	United States

Citation

Journal: To Be Published
Title: The influence of EF-P on mRNA reading frame maintenance
Authors: Kimbrough EM / Nguyen HA / Li H / Gamper H / Hou YM / Gonzalez RL / Dunham CM

History

Deposition	Jan 30, 2024	-
Header (metadata) release	Jul 30, 2025	-
Map release	Jul 30, 2025	-
Update	Jul 30, 2025	-
Current status	Jul 30, 2025	Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images	emd_43549.png

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Downloads & links

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EMDB archive

Map data	emd_43549.map.gz	410.9 MB		EMDB map data format
Header (meta data)	emd-43549-v30.xml emd-43549.xml	72.7 KB 72.7 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_43549_fsc.xml	18.1 KB	Display	FSC data file
Images	emd_43549.png	61.9 KB
Filedesc metadata	emd-43549.cif.gz	14 KB
Others	emd_43549_additional_1.map.gz emd_43549_half_map_1.map.gz emd_43549_half_map_2.map.gz	476.5 MB 411.7 MB 411 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-43549 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-43549	HTTPS FTP

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Validation report

Summary document	emd_43549_validation.pdf.gz	1.2 MB	Display	EMDB validaton report
Full document	emd_43549_full_validation.pdf.gz	1.2 MB	Display
Data in XML	emd_43549_validation.xml.gz	26.3 KB	Display
Data in CIF	emd_43549_validation.cif.gz	35.2 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43549 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-43549	HTTPS FTP

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Related structure data

Related structure data	8vv4MC 43535C 8vupC M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (55) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (37) #157 - Jan 2013 Transfer-Messenger RNA similarity (37) #121 - Jan 2010 70S Ribosomes similarity (12)

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Map

File

Download / File: emd_43549.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Annotation

E. coli 70S ribosome with unmodified Lys-tRNAPro(GGG) in the P/P conformation on a slippery CCC-C codon

Projections & slices

Image control

Size
Brightness
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Others	InvertY flip

Axes	Z (Sec.)	Y (Row.)	X (Col.)
	1.07 Å/pix. x 512 pix. = 547.379 Å	1.07 Å/pix. x 512 pix. = 547.379 Å	1.07 Å/pix. x 512 pix. = 547.379 Å
Surface
Projections
Slices (1/3)
Slices (1/2)
Slices (2/3)

Images are generated by Spider.

Voxel size

X=Y=Z: 1.0691 Å

Density

Histogram

Histogram (log scale)

Contour Level	By AUTHOR: 0.00281
Minimum - Maximum	-0.014840894 - 0.026294101
Average (Standard dev.)	0.000005700198 (±0.0012055261)

Symmetry

Space group: 1

Details

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Supplemental data

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Additional map: Additional Map

File

emd_43549_additional_1.map

Annotation

Additional Map

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

File

emd_43549_half_map_1.map

Annotation

Half map A

Projections & Slices

Axes	Z	Y	X
Projections
Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

File

emd_43549_half_map_2.map

Annotation

Half map B

Projections & Slices

Axes	Z	Y	X
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Slices (1/2)

Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : E.coli 70S complex with mRNA and e*/E-tRNA

Entire	Name: E.coli 70S complex with mRNA and e*/E-tRNA
Components	Complex: E.coli 70S complex with mRNA and e/E-tRNA RNA: 23S ribosomal RNA RNA: 16S ribosomal RNA RNA: 5S ribosomal RNA RNA: mRNA Protein or peptide: 50S ribosomal protein L2 Protein or peptide: 50S ribosomal protein L3 Protein or peptide: 50S ribosomal protein L4 Protein or peptide: 50S ribosomal protein L5 Protein or peptide: 50S ribosomal protein L6 Protein or peptide: Large ribosomal subunit protein bL9 Protein or peptide: 50S ribosomal protein L13 Protein or peptide: 50S ribosomal protein L14 Protein or peptide: 50S ribosomal protein L15 Protein or peptide: 50S ribosomal protein L16 Protein or peptide: Large ribosomal subunit protein bL17 Protein or peptide: 50S ribosomal protein L18 Protein or peptide: 50S ribosomal protein L19 Protein or peptide: 50S ribosomal protein L20 Protein or peptide: Ribosomal protein L21 Protein or peptide: 50S ribosomal protein L22 Protein or peptide: 50S ribosomal protein L23 Protein or peptide: 50S ribosomal protein L24 Protein or peptide: 50S ribosomal protein L25 Protein or peptide: Large ribosomal subunit protein bL27 Protein or peptide: 50S ribosomal protein L28 Protein or peptide: 50S ribosomal protein L29 Protein or peptide: Large ribosomal subunit protein uL30 Protein or peptide: 50S ribosomal protein L31 Protein or peptide: 50S ribosomal protein L32 Protein or peptide: Large ribosomal subunit protein bL33 Protein or peptide: 50S ribosomal protein L34 Protein or peptide: 50S ribosomal protein L35 Protein or peptide: 50S ribosomal protein L36 Protein or peptide: 30S ribosomal protein S2 Protein or peptide: 30S ribosomal protein S3 Protein or peptide: 30S ribosomal protein S4 Protein or peptide: 30S ribosomal protein S5 Protein or peptide: 30S ribosomal protein S6 Protein or peptide: 30S ribosomal protein S7 Protein or peptide: 30S ribosomal protein S8 Protein or peptide: Small ribosomal subunit protein uS9 Protein or peptide: 30S ribosomal protein S10 Protein or peptide: 30S ribosomal protein S11 Protein or peptide: Small ribosomal subunit protein uS12 Protein or peptide: 30S ribosomal protein S13 Protein or peptide: Small ribosomal subunit protein uS14 Protein or peptide: Small ribosomal subunit protein uS15 Protein or peptide: 30S ribosomal protein S16 Protein or peptide: Small ribosomal subunit protein uS17 Protein or peptide: 30S ribosomal protein S18 Protein or peptide: 30S ribosomal protein S19 Protein or peptide: 30S ribosomal protein S20 Protein or peptide: 30S ribosomal protein S21 RNA: tRNA^ProL (GGG) Ligand: MAGNESIUM ION Ligand: LYSINE Ligand*: water

Entire

Name: E.coli 70S complex with mRNA and e*/E-tRNA

Components

Complex: E.coli 70S complex with mRNA and e*/E-tRNA
- RNA: 23S ribosomal RNA
- RNA: 16S ribosomal RNA
- RNA: 5S ribosomal RNA
- RNA: mRNA
- Protein or peptide: 50S ribosomal protein L2
- Protein or peptide: 50S ribosomal protein L3
- Protein or peptide: 50S ribosomal protein L4
- Protein or peptide: 50S ribosomal protein L5
- Protein or peptide: 50S ribosomal protein L6
- Protein or peptide: Large ribosomal subunit protein bL9
- Protein or peptide: 50S ribosomal protein L13
- Protein or peptide: 50S ribosomal protein L14
- Protein or peptide: 50S ribosomal protein L15
- Protein or peptide: 50S ribosomal protein L16
- Protein or peptide: Large ribosomal subunit protein bL17
- Protein or peptide: 50S ribosomal protein L18
- Protein or peptide: 50S ribosomal protein L19
- Protein or peptide: 50S ribosomal protein L20
- Protein or peptide: Ribosomal protein L21
- Protein or peptide: 50S ribosomal protein L22
- Protein or peptide: 50S ribosomal protein L23
- Protein or peptide: 50S ribosomal protein L24
- Protein or peptide: 50S ribosomal protein L25
- Protein or peptide: Large ribosomal subunit protein bL27
- Protein or peptide: 50S ribosomal protein L28
- Protein or peptide: 50S ribosomal protein L29
- Protein or peptide: Large ribosomal subunit protein uL30
- Protein or peptide: 50S ribosomal protein L31
- Protein or peptide: 50S ribosomal protein L32
- Protein or peptide: Large ribosomal subunit protein bL33
- Protein or peptide: 50S ribosomal protein L34
- Protein or peptide: 50S ribosomal protein L35
- Protein or peptide: 50S ribosomal protein L36
- Protein or peptide: 30S ribosomal protein S2
- Protein or peptide: 30S ribosomal protein S3
- Protein or peptide: 30S ribosomal protein S4
- Protein or peptide: 30S ribosomal protein S5
- Protein or peptide: 30S ribosomal protein S6
- Protein or peptide: 30S ribosomal protein S7
- Protein or peptide: 30S ribosomal protein S8
- Protein or peptide: Small ribosomal subunit protein uS9
- Protein or peptide: 30S ribosomal protein S10
- Protein or peptide: 30S ribosomal protein S11
- Protein or peptide: Small ribosomal subunit protein uS12
- Protein or peptide: 30S ribosomal protein S13
- Protein or peptide: Small ribosomal subunit protein uS14
- Protein or peptide: Small ribosomal subunit protein uS15
- Protein or peptide: 30S ribosomal protein S16
- Protein or peptide: Small ribosomal subunit protein uS17
- Protein or peptide: 30S ribosomal protein S18
- Protein or peptide: 30S ribosomal protein S19
- Protein or peptide: 30S ribosomal protein S20
- Protein or peptide: 30S ribosomal protein S21
RNA: tRNA^ProL (GGG)
Ligand: MAGNESIUM ION
Ligand: LYSINE
Ligand: water

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Supramolecule #1: E.coli 70S complex with mRNA and e*/E-tRNA

Supramolecule	Name: E.coli 70S complex with mRNA and e/E-tRNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list*: #1-#4, #6-#54
Source (natural)	Organism: Escherichia coli (E. coli)

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Macromolecule #1: 23S ribosomal RNA

Macromolecule	Name: 23S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 941.32925 KDa
Sequence	String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG ...String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAUGUCGAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCAAUC AAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACUGU UUCGGCAAGG GGGUCAACC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACACG GCGGGUGCUA A CGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUGGGAA ACGAUGUGGG AA GGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCACUGG UCGAGUCGGC CUG CGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCUUAU GCGUUGUUGG GUAGGGGAGC GUUC UGUAA GCCUGCGAAG GUGUGCUGUG AGGCAUGCUG GAGGUAUCAG AAGUGCGAAU GCUGACAUAA GUAACGAUAA AGCGG GUGA AAAGCCCGCU CGCCGGAAGA CCAAGGGUUC CUGUCCAACG UUAAUCGGGG CAGGGUGAGU CGACCCCUAA GGCGAG GCC GAAAGGCGUA GUCGAUGGGA AACAGGUUAA UAUUCCUGUA CUUGGUGUUA CUGCGAAGGG GGGACGGAGA AGGCUAU GU UGGCCGGGCG ACGGUUGUCC CGGUUUAAGC GUGUAGGCUG GUUUUCCAGG CAAAUCCGGA AAAUCAAGGC UGAGGCGU G AUGACGAGGC ACUACGGUGC UGAAGCAACA AAUGCCCUGC UUCCAGGAAA AGCCUCUAAG CAUCAGGUAA CAUCAAAUC GUACCCCAAA CCGACACAGG UGGUCAGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGAAGGAAC UAGGCAAAAU GGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA GCUGAAAUCA GUCGAAGAUA C CAGCUGGC UGCAACUGUU UAUUAAAAAC ACAGCACUGU GCAAACACGA AAGUGGACGU AUACGGUGUG ACGCCUGCCC GG UGCCGGG AGGUUAAUUG AUGGGGUUAG CGCAAGCGAA GCUCUUGAUC GAAGCCCCGG UAAACGGCGG CCGUAACUAU AAC GGUCCU AAGGUAGCGA AAUUCCUUGU CGGGUAAGUU CCGACCUGCA CGAAUGGCGU AAUGAUGGCC AGGCUGUCUC CACC CGAGA CUCAGUGAAA UUGAACUCGC UGUGAAGAUG CAGUGUACCC GCGGCAAGAC GGAAAGACCC CGUAAACCUU UACUA UAGC UUGACACUGA ACAUUGAGCU UGAUGUGUAG GAUAGGUGGG AGGCUUUGAA GUGUGGACGC CAGUCUGCAU GGAGCC GAC CUUGAAAUAC CACCCUUUAA UGUUUGAUGU UCUAACGUUG ACCCGUAAUC CGGGUUGCGG ACAGUGUCUG GUGGGUA GU UUGACUGGGG CGGUCUCCUC CUAAAGAGUA ACGGAGGAGC ACGAAGGUUG GCUAAUCCUG GUCGGACAUC AGGAGGUU A GUGCAAUGGC AUAAGCCAGC UUGACUGCGA GCGUGACGGC GCGAGCAGGU GCGAAAGCAG GUCAUAGUGA UCCGGUGGU UCUGAAUGGA AGGGCCAUCG CUCAACGGAU AAAAGGUACU CCGGGGAUAA CAGGCUGAUA CCGCCCAAGA GUUCAUAUCG ACGGCGGUG UUUGGCACCU CGAUGUCGGC UCAUCACAUC CUGGGGCUGA AGUAGGUCCC AAGGGUAUGG CUGUUCGCCA U UUAAAGUG GUACGCGAGC UGGGUUUAGA ACGUCGUGAG ACAGUUCGGU CCCUAUCUGC CGUGGGCGCU GGAGAACUGA GG GGGGCUG CUCCUAGUAC GAGAGGACCG GAGUGGACGC AUCACUGGUG UUCGGGUUGU CAUGCCAAUG GCACUGCCCG GUA GCUAAA UGCGGAAGAG AUAAGUGCUG AAAGCAUCUA AGCACGAAAC UUGCCCCGAG AUGAGUUCUC CCUGACCCUU UAAG GGUCC UGAAGGAACG UUGAAGACGA CGACGUUGAU AGGCCGGGUG UGUAAGCGCA GCGAUGCGUU GAGCUAACCG GUACU AAUG AACCGUGAGG CUUAACCUU GENBANK: GENBANK: CP062245.1

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Macromolecule #2: 16S ribosomal RNA

Macromolecule	Name: 16S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 499.238 KDa
Sequence	String: AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG ...String: AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG CUAAUACCGC AUAACGUCGC AAGACCAAAG AGGGGGACCU UCGGGCCUCU UGCCAUCGGA UGUGCCCAGA UG GGAUUAG CUAGUAGGUG GGGUAACGGC UCACCUAGGC GACGAUCCCU AGCUGGUCUG AGAGGAUGAC CAGCCACACU GGA ACUGAG ACACGGUCCA GACUCCUACG GGAGGCAGCA GUGGGGAAUA UUGCACAAUG GGCGCAAGCC UGAUGCAGCC AUGC CGCGU GUAUGAAGAA GGCCUUCGGG UUGUAAAGUA CUUUCAGCGG GGAGGAAGGG AGUAAAGUUA AUACCUUUGC UCAUU GACG UUACCCGCAG AAGAAGCACC GGCUAACUCC G(PSU)GCCAGCAG CC(G7M)CGGUAAU ACGGAGGGUG CAAGCGUU A AUCGGAAUUA CUGGGCGUAA AGCGCACGCA GGCGGUUUGU UAAGUCAGAU GUGAAAUCCC CGGGCUCAAC CUGGGAACU GCAUCUGAUA CUGGCAAGCU UGAGUCUCGU AGAGGGGGGU AGAAUUCCAG GUGUAGCGGU GAAAUGCGUA GAGAUCUGGA GGAAUACCG GUGGCGAAGG CGGCCCCCUG GACGAAGACU GACGCUCAGG UGCGAAAGCG UGGGGAGCAA ACAGGAUUAG A UACCCUGG UAGUCCACGC CGUAAACGAU GUCGACUUGG AGGUUGUGCC CUUGAGGCGU GGCUUCCGGA GCUAACGCGU UA AGUCGAC CGCCUGGGGA GUACGGCCGC AAGGUUAAAA CUCAAAUGAA UUGACGGGGG CCCGCACAAG CGGUGGAGCA UGU GGUUUA AUUCGAU(2MG)(5MC)A ACGCGAAGAA CCUUACCUGG UCUUGACAUC CACGGAAGUU UUCAGAGAUG AGAAUG UGC CUUCGGGAAC CGUGAGACAG GUGCUGCAUG GCUGUCGUCA GCUCGUGUUG UGAAAUGUUG GGUUAAGUCC CGCAACG AG CGCAACCCUU AUCCUUUGUU GCCAGCGGUC CGGCCGGGAA CUCAAAGGAG ACUGCCAGUG AUAAACUGGA GGAAGGUG G GGAUGACGUC AAGUCAUCAU G(2MG)CCCUUACG ACCAGGGCUA CACACGUGCU ACAAUGGCGC AUACAAAGAG AAGCG ACCU CGCGAGAGCA AGCGGACCUC AUAAAGUGCG UCGUAGUCCG GAUUGGAGUC UGCAACUCGA CUCCAUGAAG UCGGAA UCG CUAGUAAUCG UGGAUCAGAA UGCCACGGUG AAUACGUUCC CGGGCCUUGU ACACACCG(4OC)C CGU(5MC)ACACC AUGGGAGUGG GUUGCAAAAG AAGUAGGUAG CUUAACCUUC GGGAGGGCGC UUACCACUUU GUGAUUCAUG ACUGGGGUGA AGUCG(UR3)AAC AAGGUAACCG UAGG(2MG)G(MA6)(MA6)CC UGCGGUUGGA UCACCUCCU GENBANK: GENBANK: CP029180.1

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Macromolecule #3: 5S ribosomal RNA

Macromolecule	Name: 5S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 38.79009 KDa
Sequence	String: UGCCUGGCGG CCGUAGCGCG GUGGUCCCAC CUGACCCCAU GCCGAACUCA GAAGUGAAAC GCCGUAGCGC CGAUGGUAGU GUGGGGUCU CCCCAUGCGA GAGUAGGGAA CUGCCAGGCA U GENBANK: GENBANK: CP024273.1

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Macromolecule #4: mRNA

Macromolecule	Name: mRNA / type: rna / ID: 4 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 5.700467 KDa
Sequence	String: CAUAAAAAUG CCCCGUUA

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Macromolecule #5: tRNA^ProL (GGG)

Macromolecule	Name: tRNA^ProL (GGG) / type: rna / ID: 5 / Number of copies: 1
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 24.876777 KDa
Sequence	String: CGGCACGUAG CGCAGCCUGG UAGCGCACCG UCAUGGGGUG UCGGGGGUCG GAGGUUCAAA UCCUCUCGUG CCGACCA GENBANK: GENBANK: CP053723.1

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Macromolecule #6: 50S ribosomal protein L2

Macromolecule	Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 29.923619 KDa
Sequence	String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD ...String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK UniProtKB: Large ribosomal subunit protein uL2

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Macromolecule #7: 50S ribosomal protein L3

Macromolecule	Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 22.277535 KDa
Sequence	String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE ...String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #8: 50S ribosomal protein L4

Macromolecule	Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 22.121566 KDa
Sequence	String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD ...String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #9: 50S ribosomal protein L5

Macromolecule	Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 20.333611 KDa
Sequence	String: MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #10: 50S ribosomal protein L6

Macromolecule	Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 18.932791 KDa
Sequence	String: MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #11: Large ribosomal subunit protein bL9

Macromolecule	Name: Large ribosomal subunit protein bL9 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.78902 KDa
Sequence	String: MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE UniProtKB: Large ribosomal subunit protein bL9

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Macromolecule #12: 50S ribosomal protein L13

Macromolecule	Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 16.050606 KDa
Sequence	String: MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #13: 50S ribosomal protein L14

Macromolecule	Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.565067 KDa
Sequence	String: MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #14: 50S ribosomal protein L15

Macromolecule	Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.008471 KDa
Sequence	String: MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #15: 50S ribosomal protein L16

Macromolecule	Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.312269 KDa
Sequence	String: MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV RMGKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #16: Large ribosomal subunit protein bL17

Macromolecule	Name: Large ribosomal subunit protein bL17 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 14.393657 KDa
Sequence	String: MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE UniProtKB: Large ribosomal subunit protein bL17

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Macromolecule #17: 50S ribosomal protein L18

Macromolecule	Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 12.794668 KDa
Sequence	String: MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF UniProtKB: UNIPROTKB: S1E9N8

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Macromolecule #18: 50S ribosomal protein L19

Macromolecule	Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.159278 KDa
Sequence	String: MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN UniProtKB: Large ribosomal subunit protein bL19

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Macromolecule #19: 50S ribosomal protein L20

Macromolecule	Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.528024 KDa
Sequence	String: MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA UniProtKB: Large ribosomal subunit protein bL20

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Macromolecule #20: Ribosomal protein L21

Macromolecule	Name: Ribosomal protein L21 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.586374 KDa
Sequence	String: MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA UniProtKB: Large ribosomal subunit protein bL21

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Macromolecule #21: 50S ribosomal protein L22

Macromolecule	Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 12.253359 KDa
Sequence	String: METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R UniProtKB: Large ribosomal subunit protein uL22

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Macromolecule #22: 50S ribosomal protein L23

Macromolecule	Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.22216 KDa
Sequence	String: MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E UniProtKB: UNIPROTKB: U9Y6C4

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Macromolecule #23: 50S ribosomal protein L24

Macromolecule	Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.33925 KDa
Sequence	String: MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #24: 50S ribosomal protein L25

Macromolecule	Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 10.713465 KDa
Sequence	String: MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA UniProtKB: Large ribosomal subunit protein bL25

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Macromolecule #25: Large ribosomal subunit protein bL27

Macromolecule	Name: Large ribosomal subunit protein bL27 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.015344 KDa
Sequence	String: AHKKAGGSTR NGRDSEAKRL GVKRFGGESV LAGSIIVRQR GTKFHAGANV GCGRDHTLFA KADGKVKFEV KGPKNRKFIS IEAE UniProtKB: Large ribosomal subunit protein bL27

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Macromolecule #26: 50S ribosomal protein L28

Macromolecule	Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.027551 KDa
Sequence	String: MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY UniProtKB: Large ribosomal subunit protein bL28

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Macromolecule #27: 50S ribosomal protein L29

Macromolecule	Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.286464 KDa
Sequence	String: MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #28: Large ribosomal subunit protein uL30

Macromolecule	Name: Large ribosomal subunit protein uL30 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.55482 KDa
Sequence	String: MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #29: 50S ribosomal protein L31

Macromolecule	Name: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.887117 KDa
Sequence	String: MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD VATGGRVDRF NKRFNIPGSK UniProtKB: Large ribosomal subunit protein bL31

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Macromolecule #30: 50S ribosomal protein L32

Macromolecule	Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.463445 KDa
Sequence	String: MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK UniProtKB: Large ribosomal subunit protein bL32

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Macromolecule #31: Large ribosomal subunit protein bL33

Macromolecule	Name: Large ribosomal subunit protein bL33 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 6.388631 KDa
Sequence	String: MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK UniProtKB: Large ribosomal subunit protein bL33

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Macromolecule #32: 50S ribosomal protein L34

Macromolecule	Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 5.397463 KDa
Sequence	String: MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK UniProtKB: Large ribosomal subunit protein bL34

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Macromolecule #33: 50S ribosomal protein L35

Macromolecule	Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 7.313032 KDa
Sequence	String: MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA UniProtKB: Large ribosomal subunit protein bL35

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Macromolecule #34: 50S ribosomal protein L36

Macromolecule	Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 4.37739 KDa
Sequence	String: MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG UniProtKB: Large ribosomal subunit protein bL36

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Macromolecule #35: 30S ribosomal protein S2

Macromolecule	Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 26.78167 KDa
Sequence	String: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH ...String: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #36: 30S ribosomal protein S3

Macromolecule	Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 26.031316 KDa
Sequence	String: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY ...String: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #37: 30S ribosomal protein S4

Macromolecule	Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 23.514199 KDa
Sequence	String: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP ...String: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #38: 30S ribosomal protein S5

Macromolecule	Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 17.629398 KDa
Sequence	String: MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #39: 30S ribosomal protein S6

Macromolecule	Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 15.727512 KDa
Sequence	String: MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #40: 30S ribosomal protein S7

Macromolecule	Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 20.055156 KDa
Sequence	String: MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #41: 30S ribosomal protein S8

Macromolecule	Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 14.146557 KDa
Sequence	String: MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRK DELPKVMAGL GIAVVSTSKG VMTDRAARQA GLGGEIICYV A UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #42: Small ribosomal subunit protein uS9

Macromolecule	Name: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 14.88627 KDa
Sequence	String: MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #43: 30S ribosomal protein S10

Macromolecule	Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.755597 KDa
Sequence	String: MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #44: 30S ribosomal protein S11

Macromolecule	Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.870975 KDa
Sequence	String: MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP FAAQVAAERC ADAVKEYGIK NLEVMVKGP GPGRESTIRA LNAAGFRITN ITDVTPIPHN GCRPPKKRRV UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #45: Small ribosomal subunit protein uS12

Macromolecule	Name: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.814249 KDa
Sequence	String: MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVK(0TD) LPGVRYHTVR GALDCSGVKD RKQARSKYGV KRPKA UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #46: 30S ribosomal protein S13

Macromolecule	Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 13.128467 KDa
Sequence	String: MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK UniProtKB: Small ribosomal subunit protein uS13

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Macromolecule #47: Small ribosomal subunit protein uS14

Macromolecule	Name: Small ribosomal subunit protein uS14 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 11.60656 KDa
Sequence	String: MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAM RGEIPGLKKA SW UniProtKB: Small ribosomal subunit protein uS14

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Macromolecule #48: Small ribosomal subunit protein uS15

Macromolecule	Name: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 10.290816 KDa
Sequence	String: MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV SQRRKLLDYL KRKDVARYTQ LIERLGLRR UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #49: 30S ribosomal protein S16

Macromolecule	Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.207572 KDa
Sequence	String: MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #50: Small ribosomal subunit protein uS17

Macromolecule	Name: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.724491 KDa
Sequence	String: MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL UniProtKB: Small ribosomal subunit protein uS17

+
Macromolecule #51: 30S ribosomal protein S18

Macromolecule	Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.005472 KDa
Sequence	String: MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ UniProtKB: Small ribosomal subunit protein bS18

+
Macromolecule #52: 30S ribosomal protein S19

Macromolecule	Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 10.455355 KDa
Sequence	String: MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV FVTDEMVGHK LGEFAPTRTY RGHAADKKA KKK UniProtKB: Small ribosomal subunit protein uS19

+
Macromolecule #53: 30S ribosomal protein S20

Macromolecule	Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 9.708464 KDa
Sequence	String: MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR QAAKGLIHKN KAARHKANLT AQINKLA UniProtKB: Small ribosomal subunit protein bS20

+
Macromolecule #54: 30S ribosomal protein S21

Macromolecule	Name: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli (E. coli)
Molecular weight	Theoretical: 8.524039 KDa
Sequence	String: MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y UniProtKB: Small ribosomal subunit protein bS21

+
Macromolecule #55: MAGNESIUM ION

+
Macromolecule #56: LYSINE

+
Macromolecule #57: water

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer	pH: 7.6
Vitrification	Cryogen name: ETHANE

-
Electron microscopy

Microscope	FEI TITAN KRIOS
Image recording	Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 56.07 e/Å²
Electron beam	Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.6 µm
Experimental equipment	Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

CTF correction	Type: NONE
Startup model	Type of model: PDB ENTRY PDB model - PDB ID: 8urm
Final reconstruction	Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 29634
Initial angle assignment	Type: MAXIMUM LIKELIHOOD
Final angle assignment	Type: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

+
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Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 55 / Number of copies: 424 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

Macromolecule	Name: LYSINE / type: ligand / ID: 56 / Number of copies: 1 / Formula: LYS
Molecular weight	Theoretical: 147.195 Da
Chemical component information	ChemComp-LYS: LYSINE

Macromolecule	Name: water / type: ligand / ID: 57 / Number of copies: 560 / Formula: HOH
Molecular weight	Theoretical: 18.015 Da
Chemical component information	ChemComp-HOH: WATER

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-Sample components

+Entire : E.coli 70S complex with mRNA and e*/E-tRNA

+Supramolecule #1: E.coli 70S complex with mRNA and e*/E-tRNA

+Macromolecule #1: 23S ribosomal RNA

+Macromolecule #2: 16S ribosomal RNA

+Macromolecule #3: 5S ribosomal RNA

+Macromolecule #4: mRNA

+Macromolecule #5: tRNA^ProL (GGG)

+Macromolecule #6: 50S ribosomal protein L2

+Macromolecule #7: 50S ribosomal protein L3

+Macromolecule #8: 50S ribosomal protein L4

+Macromolecule #9: 50S ribosomal protein L5

+Macromolecule #10: 50S ribosomal protein L6

+Macromolecule #11: Large ribosomal subunit protein bL9

+Macromolecule #12: 50S ribosomal protein L13

+Macromolecule #13: 50S ribosomal protein L14

+Macromolecule #14: 50S ribosomal protein L15

+Macromolecule #15: 50S ribosomal protein L16

+Macromolecule #16: Large ribosomal subunit protein bL17

+Macromolecule #17: 50S ribosomal protein L18

+Macromolecule #18: 50S ribosomal protein L19

+Macromolecule #19: 50S ribosomal protein L20

+Macromolecule #20: Ribosomal protein L21

+Macromolecule #21: 50S ribosomal protein L22

+Macromolecule #22: 50S ribosomal protein L23

+Macromolecule #23: 50S ribosomal protein L24

+Macromolecule #24: 50S ribosomal protein L25

+Macromolecule #25: Large ribosomal subunit protein bL27

+Macromolecule #26: 50S ribosomal protein L28

+Macromolecule #27: 50S ribosomal protein L29

+Macromolecule #28: Large ribosomal subunit protein uL30

+Macromolecule #29: 50S ribosomal protein L31

+Macromolecule #30: 50S ribosomal protein L32

+Macromolecule #31: Large ribosomal subunit protein bL33

+Macromolecule #32: 50S ribosomal protein L34

+Macromolecule #33: 50S ribosomal protein L35

+Macromolecule #34: 50S ribosomal protein L36

+Macromolecule #35: 30S ribosomal protein S2

+Macromolecule #36: 30S ribosomal protein S3

+Macromolecule #37: 30S ribosomal protein S4

+Macromolecule #38: 30S ribosomal protein S5

+Macromolecule #39: 30S ribosomal protein S6

+Macromolecule #40: 30S ribosomal protein S7

+Macromolecule #41: 30S ribosomal protein S8

+Macromolecule #42: Small ribosomal subunit protein uS9

+Macromolecule #43: 30S ribosomal protein S10

+Macromolecule #44: 30S ribosomal protein S11

+Macromolecule #45: Small ribosomal subunit protein uS12

+Macromolecule #46: 30S ribosomal protein S13

+Macromolecule #47: Small ribosomal subunit protein uS14

+Macromolecule #48: Small ribosomal subunit protein uS15

+Macromolecule #49: 30S ribosomal protein S16

+Macromolecule #50: Small ribosomal subunit protein uS17

+Macromolecule #51: 30S ribosomal protein S18

+Macromolecule #52: 30S ribosomal protein S19

+Macromolecule #53: 30S ribosomal protein S20

+Macromolecule #54: 30S ribosomal protein S21

+Macromolecule #55: MAGNESIUM ION

+Macromolecule #56: LYSINE

+Macromolecule #57: water

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options

+
Open data

-
Basic information

-
Structure visualization

-
Downloads & links

-
EMDB archive

-
Validation report

-
Related structure data

-
Links

-
Map

Image control

-
Supplemental data

-
Additional map: Additional Map

-
Half map: Half map A

-
Half map: Half map B

-
Sample components

+
Entire : E.coli 70S complex with mRNA and e*/E-tRNA

+
Supramolecule #1: E.coli 70S complex with mRNA and e*/E-tRNA

+
Macromolecule #1: 23S ribosomal RNA

+
Macromolecule #2: 16S ribosomal RNA

+
Macromolecule #3: 5S ribosomal RNA

+
Macromolecule #4: mRNA

+
Macromolecule #5: tRNA^ProL (GGG)

+
Macromolecule #6: 50S ribosomal protein L2

+
Macromolecule #7: 50S ribosomal protein L3

+
Macromolecule #8: 50S ribosomal protein L4

+
Macromolecule #9: 50S ribosomal protein L5

+
Macromolecule #10: 50S ribosomal protein L6

+
Macromolecule #11: Large ribosomal subunit protein bL9

+
Macromolecule #12: 50S ribosomal protein L13

+
Macromolecule #13: 50S ribosomal protein L14

+
Macromolecule #14: 50S ribosomal protein L15

+
Macromolecule #15: 50S ribosomal protein L16

+
Macromolecule #16: Large ribosomal subunit protein bL17

+
Macromolecule #17: 50S ribosomal protein L18

+
Macromolecule #18: 50S ribosomal protein L19

+
Macromolecule #19: 50S ribosomal protein L20

+
Macromolecule #20: Ribosomal protein L21

+
Macromolecule #21: 50S ribosomal protein L22

+
Macromolecule #22: 50S ribosomal protein L23

+
Macromolecule #23: 50S ribosomal protein L24

+
Macromolecule #24: 50S ribosomal protein L25

+
Macromolecule #25: Large ribosomal subunit protein bL27

+
Macromolecule #26: 50S ribosomal protein L28

+
Macromolecule #27: 50S ribosomal protein L29

+
Macromolecule #28: Large ribosomal subunit protein uL30

+
Macromolecule #29: 50S ribosomal protein L31

+
Macromolecule #30: 50S ribosomal protein L32

+
Macromolecule #31: Large ribosomal subunit protein bL33

+
Macromolecule #32: 50S ribosomal protein L34

+
Macromolecule #33: 50S ribosomal protein L35

+
Macromolecule #34: 50S ribosomal protein L36

+
Macromolecule #35: 30S ribosomal protein S2

+
Macromolecule #36: 30S ribosomal protein S3

+
Macromolecule #37: 30S ribosomal protein S4

+
Macromolecule #38: 30S ribosomal protein S5

+
Macromolecule #39: 30S ribosomal protein S6

+
Macromolecule #40: 30S ribosomal protein S7

+
Macromolecule #41: 30S ribosomal protein S8

+
Macromolecule #42: Small ribosomal subunit protein uS9

+
Macromolecule #43: 30S ribosomal protein S10

+
Macromolecule #44: 30S ribosomal protein S11

+
Macromolecule #45: Small ribosomal subunit protein uS12

+
Macromolecule #46: 30S ribosomal protein S13

+
Macromolecule #47: Small ribosomal subunit protein uS14

+
Macromolecule #48: Small ribosomal subunit protein uS15

+
Macromolecule #49: 30S ribosomal protein S16

+
Macromolecule #50: Small ribosomal subunit protein uS17

+
Macromolecule #51: 30S ribosomal protein S18

+
Macromolecule #52: 30S ribosomal protein S19

+
Macromolecule #53: 30S ribosomal protein S20

+
Macromolecule #54: 30S ribosomal protein S21

+
Macromolecule #55: MAGNESIUM ION

+
Macromolecule #56: LYSINE

+
Macromolecule #57: water