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- EMDB-43301: Constituent EM map: Focused refinement JSol+CSol+S100A1 of mouse ... -
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Open data
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Basic information
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Title | Constituent EM map: Focused refinement JSol+CSol+S100A1 of mouse RyR1 in complex with S100A1 (high-Ca2+/CFF/ATP dataset) | |||||||||
![]() | Constituent EM map: Focused refinement JSol/CSol/S100A1 of mouse RyR1 in complex with S100A1 (high-Ca/CFF/ATP dataset) | |||||||||
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![]() | Calcium / Ion Channel / Complex / MEMBRANE PROTEIN | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.29 Å | |||||||||
![]() | Weninger G / Marks AR | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the regulation of RyR1 by S100A1. Authors: Weninger G | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 255.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.6 KB 14.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 17 KB | Display | ![]() |
Images | ![]() | 30.9 KB | ||
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 474.3 MB 474.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 26 KB | Display | |
Data in CIF | ![]() | 34 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Constituent EM map: Focused refinement JSol/CSol/S100A1 of mouse RyR1 in complex with S100A1 (high-Ca/CFF/ATP dataset) | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.83 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map B
File | emd_43301_half_map_1.map | ||||||||||||
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Annotation | Half Map B | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map A
File | emd_43301_half_map_2.map | ||||||||||||
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Annotation | Half Map A | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Complex of RyR1 with Calstabin-1 and S100A1 (high-Ca2+/CFF/ATP)
Entire | Name: Complex of RyR1 with Calstabin-1 and S100A1 (high-Ca2+/CFF/ATP) |
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Components |
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-Supramolecule #1: Complex of RyR1 with Calstabin-1 and S100A1 (high-Ca2+/CFF/ATP)
Supramolecule | Name: Complex of RyR1 with Calstabin-1 and S100A1 (high-Ca2+/CFF/ATP) type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 / Details: 0.25 mM free Ca2+; 5 mM Caffeine; 10 mM ATP |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 8.5 mg/mL | |||||||||||||||||||||
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Buffer | pH: 7.4 Component:
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Grid | Model: Quantifoil R0.6/1 / Material: GOLD / Mesh: 300 | |||||||||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV | |||||||||||||||||||||
Details | 0.15 mmol/L S100A1-dimer |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 1 / Number real images: 12555 / Average electron dose: 58.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.2 µm / Nominal defocus min: 0.5 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |