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- EMDB-43227: DNA origami colloid for self-assembly of tubules: (10,0) monomer -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | DNA origami colloid for self-assembly of tubules: (10,0) monomer | |||||||||
![]() | Primary map of the (10,0) monomer. | |||||||||
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![]() | DNA origami / synthetic construct / self-assembly / DNA | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 20.88 Å | |||||||||
![]() | Videbaek TE / Rogers WB | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Economical routes to size-specific assembly of self-closing structures Authors: Videbaek TE / Hayakawa D / Grason GM / Hagan MF / Fraden S / Rogers WB | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 162.3 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.7 KB 13.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13 KB | Display | ![]() |
Images | ![]() | 62.7 KB | ||
Masks | ![]() | 178 MB | ![]() | |
Filedesc metadata | ![]() | 3.9 KB | ||
Others | ![]() ![]() | 140.7 MB 140.8 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 20.1 KB | Display | |
Data in CIF | ![]() | 26.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Primary map of the (10,0) monomer. | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.023 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Half-map of the structure.
File | emd_43227_half_map_1.map | ||||||||||||
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Annotation | Half-map of the structure. | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half-map of the structure.
File | emd_43227_half_map_2.map | ||||||||||||
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Annotation | Half-map of the structure. | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : DNA origami colloid for self-assembly of tubules: (10,0) monomer
Entire | Name: DNA origami colloid for self-assembly of tubules: (10,0) monomer |
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Components |
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-Supramolecule #1: DNA origami colloid for self-assembly of tubules: (10,0) monomer
Supramolecule | Name: DNA origami colloid for self-assembly of tubules: (10,0) monomer type: complex / ID: 1 / Parent: 0 |
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Molecular weight | Theoretical: 5.2 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Grid | Model: C-flat-1.2/1.3 / Material: COPPER / Mesh: 400 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK I |
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Electron microscopy
Microscope | TFS TUNDRA |
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Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 775 / Average exposure time: 1.4 sec. / Average electron dose: 69.8 e/Å2 |
Electron beam | Acceleration voltage: 100 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 1.6 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 0.5 µm |
Sample stage | Cooling holder cryogen: NITROGEN |