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- EMDB-43108: Reconstruction of human Slo1 in complex with human LRRC26 in EDTA -

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Basic information

Entry
Database: EMDB / ID: EMD-43108
TitleReconstruction of human Slo1 in complex with human LRRC26 in EDTA
Map dataunsharpened map
Sample
  • Complex: Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits
    • Protein or peptide: Calcium-activated potassium channel subunit alpha-1
    • Protein or peptide: Leucine-rich repeat-containing protein 26
KeywordsSlo1 / BK / maxiK / LRRC26 / Gamma1 / closed / resting VSD / channel / TRANSPORT PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.01 Å
AuthorsPal K / Kallure GS / Chowdhury S
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM145719 United States
CitationJournal: To Be Published
Title: Reconstruction of human Slo1 in complex with human LRRC26 in EDTA
Authors: Pal K / Kallure GS / Chowdhury S
History
DepositionDec 11, 2023-
Header (metadata) releaseDec 25, 2024-
Map releaseDec 25, 2024-
UpdateDec 25, 2024-
Current statusDec 25, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_43108.png

Downloads & links

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Map

FileDownload / File: emd_43108.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationunsharpened map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.08 Å/pix.
x 256 pix.
= 276.48 Å		1.08 Å/pix.
x 256 pix.
= 276.48 Å		1.08 Å/pix.
x 256 pix.
= 276.48 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.08 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.09
Minimum - Maximum-0.09628243 - 0.3408447
Average (Standard dev.)0.0063600615 (±0.021539712)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions256256256
Spacing256256256
CellA=B=C: 276.48 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: Sharpened map

Fileemd_43108_additional_1.map
AnnotationSharpened map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map A

Fileemd_43108_half_map_1.map
AnnotationHalf map A
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half map B

Fileemd_43108_half_map_2.map
AnnotationHalf map B
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits

EntireName: Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits
Components
  • Complex: Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits
    • Protein or peptide: Calcium-activated potassium channel subunit alpha-1
    • Protein or peptide: Leucine-rich repeat-containing protein 26

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Supramolecule #1: Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits

SupramoleculeName: Hetero-octameric complex of hSlo1 (BK) channel and hLRRC26 subunits
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Calcium-activated potassium channel subunit alpha-1

MacromoleculeName: Calcium-activated potassium channel subunit alpha-1 / type: protein_or_peptide / ID: 1 / Enantiomer: DEXTRO
Source (natural)Organism: Homo sapiens (human)
SequenceString: MDALIIPVTM EVPCDSRGQR MWWAFLASSM VTFFGGLFII LLWRTLKYLW TVCCHCGGKT KEAQKINNGS SQADGTLKPV DEKEEAVAAE VGWMTSVKDW AGVMISAQTL TGRVLVVLVF ALSIGALVIY FIDSSNPIES CQNFYKDFTL QIDMAFNVFF LLYFGLRFIA ...String:
MDALIIPVTM EVPCDSRGQR MWWAFLASSM VTFFGGLFII LLWRTLKYLW TVCCHCGGKT KEAQKINNGS SQADGTLKPV DEKEEAVAAE VGWMTSVKDW AGVMISAQTL TGRVLVVLVF ALSIGALVIY FIDSSNPIES CQNFYKDFTL QIDMAFNVFF LLYFGLRFIA ANDKLWFWLE VNSVVDFFTV PPVFVSVYLN RSWLGLRFLR ALRLIQFSEI LQFLNILKTS NSIKLVNLLS IFISTWLTAA GFIHLVENSG DPWENFQNNQ ALTYWECVYL LMVTMSTVGY GDVYAKTTLG RLFMVFFILG GLAMFASYVP EIIELIGNRK KYGGSYSAVS GRKHIVVCGH ITLESVSNFL KDFLHKDRDD VNVEIVFLHN ISPNLELEAL FKRHFTQVEF YQGSVLNPHD LARVKIESAD ACLILANKYC ADPDAEDASN IMRVISIKNY HPKIRIITQM LQYHNKAHLL NIPSWNWKEG DDAICLAELK LGFIAQSCLA QGLSTMLANL FSMRSFIKIE EDTWQKYYLE GVSNEMYTEY LSSAFVGLSF PTVCELCFVK LKLLMIAIEY KSANRESRIL INPGNHLKIQ EGTLGFFIAS DAKEVKRAFF YCKACHDDIT DPKRIKKCGC KRLEDEQPST LSPKKKQRNG GMRNSPNTSP KLMRHDPLLI PGNDQIDNMD SNVKKYDSTG MFHWCAPKEI EKVILTRSEA AMTVLSGHVV VCIFGDVSSA LIGLRNLVMP LRASNFHYHE LKHIVFVGSI EYLKREWETL HNFPKVSILP GTPLSRADLR AVNINLCDMC VILSANQNNI DDTSLQDKEC ILASLNIKSM QFDDSIGVLQ ANSQGFTPPG MDRSSPDNSP VHGMLRQPSI TTGVNIPIIT ELVNDTNVQF LDQDDDDDPD TELYLTQPFA CGTAFAVSVL DSLMSATYFN DNILTLIRTL VTGGATPELE ALIAEENALR GGYSTPQTLA NRDRCRVAQL ALLDGPFADL GDGGCYGDLF CKALKTYNML CFGIYRLRDA HLSTPSQCTK RYVITNPPYE FELVPTDLIF CLMQFDSNSL EVLFQ

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Macromolecule #2: Leucine-rich repeat-containing protein 26

MacromoleculeName: Leucine-rich repeat-containing protein 26 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
SequenceString: MRGPSWSRPR PLLLLLLLLS PWPVWAQVSA TASPSGSLGA PDCPEVCTCV PGGLASCSAL SLPAVPPGLS LRLRALLLDH NRVRALPPGA FAGAGALQRL DLRENGLHSV HVRAFWGLGA LQLLDLSANQ LEALAPGTFA PLRALRNLSL AGNRLARLEP AALGALPLLR ...String:
MRGPSWSRPR PLLLLLLLLS PWPVWAQVSA TASPSGSLGA PDCPEVCTCV PGGLASCSAL SLPAVPPGLS LRLRALLLDH NRVRALPPGA FAGAGALQRL DLRENGLHSV HVRAFWGLGA LQLLDLSANQ LEALAPGTFA PLRALRNLSL AGNRLARLEP AALGALPLLR SLSLQDNELA ALAPGLLGRL PALDALHLRG NPWGCGCALR PLCAWLRRHP LPASEAETVL CVWPGRLTLS PLTAFSDAAF SHCAQPLALR DLAVVYTLGP ASFLVSLASC LALGSGLTAC RARRRRLRTA ALRPPRPPDP NPDPDPHGCA SPADPGSPAA AAQAAAAGLE VLFQ

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 72.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.7 µm / Nominal defocus min: 0.9 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.01 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 340094
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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