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- EMDB-43036: Myxococcus xanthus HEnc-K417N(A) protein shell with icosahedral T... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Myxococcus xanthus HEnc-K417N(A) protein shell with icosahedral T=3 symmetry | |||||||||
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![]() | encapsulin / nanocompartment / nanoparticle / cage / STRUCTURAL PROTEIN | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.91 Å | |||||||||
![]() | Hernandez C / Jenkins MC / Kopylov M | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Heterologous Prime-Boost with Immunologically Orthogonal Protein Nanoparticles for Peptide Immunofocusing Authors: Bhattacharya S / Jenkins MC / Keshavarz-Joud P / Bourque AR / White K / Alvarez Barkane AM / Bryksin A / Hernandez C / Kopylov M / Finn MG | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 30.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.6 KB 12.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 8.4 KB | Display | ![]() |
Images | ![]() | 109.5 KB | ||
Masks | ![]() | 64 MB | ![]() | |
Filedesc metadata | ![]() | 4.3 KB | ||
Others | ![]() ![]() | 58.9 MB 58.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 802 KB | Display | ![]() |
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Full document | ![]() | 801.5 KB | Display | |
Data in XML | ![]() | 15.9 KB | Display | |
Data in CIF | ![]() | 21.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 2.408 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_43036_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_43036_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Myxococcus xanthus EncA protein modified with a his-tag and K417N...
Entire | Name: Myxococcus xanthus EncA protein modified with a his-tag and K417N(A) insertion: icosahedral T1 shell |
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Components |
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-Supramolecule #1: Myxococcus xanthus EncA protein modified with a his-tag and K417N...
Supramolecule | Name: Myxococcus xanthus EncA protein modified with a his-tag and K417N(A) insertion: icosahedral T1 shell type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 6.094 MDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 4.2 mg/mL |
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Buffer | pH: 8.5 |
Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV / Details: 10 second wait, 1 second blot. |
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Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 1 / Number real images: 2971 / Average exposure time: 1.0 sec. / Average electron dose: 50.0 e/Å2 Details: NYSBC session m23aug21f, px size 1.204 A, 25 frames |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm |