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- EMDB-42785: Consensus olfactory receptor consOR51 in complex with -

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Basic information

Entry
Database: EMDB / ID: EMD-42785
TitleConsensus olfactory receptor consOR51 in complex with
Map data
Sample
  • Complex: consOR51-miniGs complex bound to Nb35
    • Other: consOR51
KeywordsOdorant / Olfaction / GPCR / Receptor / SIGNALING PROTEIN
Biological speciessynthetic construct (others)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsBillesboelle CB / Manglik A
Funding support United States, 3 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute on Deafness and Other Communication Disorders (NIH/NIDCD)R01DC020353 United States
The Vallee Foundation Inc. United States
Chan Zuckerberg Initiative United States
CitationJournal: To Be Published
Title: Engineered odorant receptors illuminate structural principles of odor discrimination
Authors: de March CA / Ma N / Billesboelle CB / Tewari J / del Torrent CL / van der Velden WJC / Faust B / Vaidehi N / Manglik A / Matsunami H
History
DepositionNov 11, 2023-
Header (metadata) releaseNov 20, 2024-
Map releaseNov 20, 2024-
UpdateNov 20, 2024-
Current statusNov 20, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_42785.png

Downloads & links

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Map

FileDownload / File: emd_42785.map.gz / Format: CCP4 / Size: 91.1 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.87 Å/pix.
x 288 pix.
= 251.424 Å		0.87 Å/pix.
x 288 pix.
= 251.424 Å		0.87 Å/pix.
x 288 pix.
= 251.424 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.873 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.8
Minimum - Maximum-1.1172005 - 2.0429146
Average (Standard dev.)0.0020380944 (±0.053516645)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions288288288
Spacing288288288
CellA=B=C: 251.42401 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #1

Fileemd_42785_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_42785_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : consOR51-miniGs complex bound to Nb35

EntireName: consOR51-miniGs complex bound to Nb35
Components
  • Complex: consOR51-miniGs complex bound to Nb35
    • Other: consOR51

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Supramolecule #1: consOR51-miniGs complex bound to Nb35

SupramoleculeName: consOR51-miniGs complex bound to Nb35 / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5
Source (natural)Organism: synthetic construct (others)
Molecular weightTheoretical: 100 KDa

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Macromolecule #1: consOR51

MacromoleculeName: consOR51 / type: other / ID: 1 / Classification: other
Source (natural)Organism: synthetic construct (others)
SequenceString: DYKDDDDASI DSINNSTSSA ATFLLTGIPG LEAAHIWISI PFCFMYLIAI LGNGTILFII RTEPSLHEPM YYFLSMLAAT DLGLSLSTL PTVLGIFWFN AREISFNACF AQMFFIHGFS FMESSVLLAM AFDRFVAICN PLRYASILTN TRVAKIGLAI L TRSFLLIL ...String:
DYKDDDDASI DSINNSTSSA ATFLLTGIPG LEAAHIWISI PFCFMYLIAI LGNGTILFII RTEPSLHEPM YYFLSMLAAT DLGLSLSTL PTVLGIFWFN AREISFNACF AQMFFIHGFS FMESSVLLAM AFDRFVAICN PLRYASILTN TRVAKIGLAI L TRSFLLIL PLPFLLKRLP YCHSNVLSHS YCLHQDVMKL ACADIRFNSI YGLFVVLSTM GLDSLLILFS YILILKTVLG IA SREERLK ALNTCVSHIC AVLVFYVPMI GLSLVHRFGK HVPPVVHVLM ANVYLLVPPV MNPIIYSVKT KQIRKRILRL FSL KKI
Recombinant expressionOrganism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 0.8 µm / Nominal magnification: 105000
Sample stageCooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 204513
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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