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- EMDB-41849: Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/200... -
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Open data
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Basic information
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Title | Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/2006(H1N1) influenza virus hemagglutinin | |||||||||||||||
![]() | A/Solomon Islands/3/2006(H1N1) hemagglutinin in complex with 18A5 Fab | |||||||||||||||
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![]() | antibody / hemagglutinin / influenza / ANTIVIRAL PROTEIN | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.81 Å | |||||||||||||||
![]() | Lei R / Wu NC | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: An explainable language model for antibody epitope prediction using curated influenza hemagglutinin antibodies Authors: Wang Y / Lv H / Lei R / Yeung Y / Shen IR / Choi D / Teo Q / Tan TJC / Gopal AB / Chen X / Graham CS / Wu NC | |||||||||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | A/Solomon Islands/3/2006(H1N1) hemagglutinin in complex with 18A5 Fab | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.48 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/200...
Entire | Name: Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/2006(H1N1) influenza virus hemagglutinin |
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Components |
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-Supramolecule #1: Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/200...
Supramolecule | Name: Structure of 310-18A5 Fab in complex with A/Solomon Islands/3/2006(H1N1) influenza virus hemagglutinin type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | filament |
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Sample preparation
Concentration | 0.05 mg/mL | |||||||||
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Buffer | pH: 8 Component:
Details: 20 mM Tris-HCl, 100 mM NaCl, pH 8.0 | |||||||||
Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 200 | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV | |||||||||
Details | with 7.5 uM LMNG |
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Electron microscopy
Microscope | TFS GLACIOS |
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Specialist optics | Phase plate: VOLTA PHASE PLATE |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 52.76 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 0.6 µm |
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Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 4.81 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 39446 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Refinement | Protocol: AB INITIO MODEL |
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