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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Cryo-EM of tri-pilus from S. islandicus REY15A | |||||||||
![]() | Cryo-EM map of tri-pilus | |||||||||
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![]() | helical symmetry / type iv pili / archaeal pilus / filament / PROTEIN FIBRIL | |||||||||
Function / homology | membrane / DUF973 family protein![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.47 Å | |||||||||
![]() | Eastep GN / Liu J / Rich-New ST / Egelman EH / Krupovic M / Wang F | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM of tri-pilus from S. islandicus REY15A Authors: Eastep GN / Liu J / Rich-New ST / Egelman EH / Krupovic M / Wang F | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 9.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13 KB 13 KB | Display Display | ![]() |
Images | ![]() | 112.8 KB | ||
Filedesc metadata | ![]() | 4.6 KB | ||
Others | ![]() ![]() | 115.9 MB 115.9 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 14.2 KB | Display | |
Data in CIF | ![]() | 16.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tibMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Cryo-EM map of tri-pilus | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.08 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map A
File | emd_41283_half_map_1.map | ||||||||||||
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Annotation | Half Map A | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map B
File | emd_41283_half_map_2.map | ||||||||||||
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Annotation | Half Map B | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : tri-pilus
Entire | Name: tri-pilus |
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Components |
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-Supramolecule #1: tri-pilus
Supramolecule | Name: tri-pilus / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: DUF973 family protein
Macromolecule | Name: DUF973 family protein / type: protein_or_peptide / ID: 1 / Number of copies: 3 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 14.053037 KDa |
Sequence | String: MNMISRRKNA KALSGAVTAL ILVIASVIIA LVVVGFAFGL FGAFTGQGTV TQVGTATLSA GTGTLTVTLK NTGAATQVTG AIINGNAAS VSGQVTISAG QNTYSISLGG ISSSTLQNLV GSTISLTLQL SNGQTVTVSA IITS UniProtKB: DUF973 family protein |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | helical reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Applied symmetry - Helical parameters - Δz: 15.8 Å Applied symmetry - Helical parameters - Δ&Phi: -39.65 ° Applied symmetry - Helical parameters - Axial symmetry: C1 (asymmetric) Resolution.type: BY AUTHOR / Resolution: 3.47 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 240930 |
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Startup model | Type of model: NONE |
Final angle assignment | Type: NOT APPLICABLE |