Human cytomegalovirus portal vertex, virion configuration 1 (VC1)
マップデータ
Post-processed map used for real space refinement.
試料
ウイルス: Human herpesvirus 5 strain AD169 (ヘルペスウイルス)
タンパク質・ペプチド: Large tegument protein deneddylase
タンパク質・ペプチド: Inner tegument protein
タンパク質・ペプチド: Capsid vertex component 2
タンパク質・ペプチド: Capsid vertex component 1
タンパク質・ペプチド: Major capsid protein
タンパク質・ペプチド: Small capsomere-interacting protein
タンパク質・ペプチド: Triplex capsid protein 1
タンパク質・ペプチド: Triplex capsid protein 2
タンパク質・ペプチド: Large structural phosphoprotein
キーワード
tegument / portal / DNA packaging / intracellular transport / VIRUS
機能・相同性
機能・相同性情報
microtubule-dependent intracellular transport of viral material towards cell periphery / host cell viral assembly compartment / T=16 icosahedral viral capsid / viral tegument / viral DNA genome packaging / viral capsid assembly / host cell cytoplasmic vesicle / virion assembly / chromosome organization / viral process ...microtubule-dependent intracellular transport of viral material towards cell periphery / host cell viral assembly compartment / T=16 icosahedral viral capsid / viral tegument / viral DNA genome packaging / viral capsid assembly / host cell cytoplasmic vesicle / virion assembly / chromosome organization / viral process / viral penetration into host nucleus / host cell / viral capsid / host cell Golgi apparatus / symbiont-mediated perturbation of host ubiquitin-like protein modification / ubiquitinyl hydrolase 1 / host cell cytoplasm / cysteine-type deubiquitinase activity / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; システインプロテアーゼ / host cell perinuclear region of cytoplasm / symbiont entry into host cell / host cell nucleus / structural molecule activity / proteolysis / DNA binding / identical protein binding 類似検索 - 分子機能
Herpesvirus tegument protein U30 / Herpes virus tegument protein U30 / Inner tegument protein / Herpesvirus UL11/UL32 / Herpesvirus large structural phosphoprotein UL32 / Small capsid protein, Herpesviridae / Small capsid protein of Herpesviridae / Large tegument protein deneddylase / Herpesvirus tegument ubiquitin-specific protease (htUSP) domain profile. / Herpesvirus large tegument protein, USP domain ...Herpesvirus tegument protein U30 / Herpes virus tegument protein U30 / Inner tegument protein / Herpesvirus UL11/UL32 / Herpesvirus large structural phosphoprotein UL32 / Small capsid protein, Herpesviridae / Small capsid protein of Herpesviridae / Large tegument protein deneddylase / Herpesvirus tegument ubiquitin-specific protease (htUSP) domain profile. / Herpesvirus large tegument protein, USP domain / Herpesvirus tegument protein, N-terminal conserved region / Herpesvirus capsid vertex component 1 / Herpesvirus UL17 protein / Herpesvirus UL25 / Herpesvirus UL25 family / Herpesvirus capsid shell protein 1 / Herpesvirus capsid shell protein VP19C / Herpesvirus capsid protein 2 / Herpesvirus VP23 like capsid protein / Herpesvirus major capsid protein / Herpesvirus major capsid protein, upper domain superfamily / Herpes virus major capsid protein / Papain-like cysteine peptidase superfamily 類似検索 - ドメイン・相同性
Large structural phosphoprotein / Capsid vertex component 2 / Triplex capsid protein 2 / Major capsid protein / Triplex capsid protein 1 / Inner tegument protein / Large tegument protein deneddylase / Capsid vertex component 1 / Small capsomere-interacting protein 類似検索 - 構成要素
National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)
DE028583
米国
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS)
T32AR071307
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
P30 AI152501
米国
James B. Pendleton Charitable Trust (to the UCLA AIDS Institute)
米国
McCarthy Family Foundation (to the UCLA AIDS Institute)
米国
National Institutes of Health/Office of the Director
1S10RR23057
米国
National Institutes of Health/Office of the Director
1S10OD018111
米国
National Science Foundation (NSF, United States)
DBI-1338135
米国
National Science Foundation (NSF, United States)
DMR-1548924
米国
引用
ジャーナル: Sci Adv / 年: 2024 タイトル: The incredible bulk: Human cytomegalovirus tegument architectures uncovered by AI-empowered cryo-EM. 著者: Jonathan Jih / Yun-Tao Liu / Wei Liu / Z Hong Zhou / 要旨: The compartmentalization of eukaryotic cells presents considerable challenges to the herpesvirus life cycle. The herpesvirus tegument, a bulky proteinaceous aggregate sandwiched between ...The compartmentalization of eukaryotic cells presents considerable challenges to the herpesvirus life cycle. The herpesvirus tegument, a bulky proteinaceous aggregate sandwiched between herpesviruses' capsid and envelope, is uniquely evolved to address these challenges, yet tegument structure and organization remain poorly characterized. We use deep-learning-enhanced cryogenic electron microscopy to investigate the tegument of human cytomegalovirus virions and noninfectious enveloped particles (NIEPs; a genome packaging-aborted state), revealing a portal-biased tegumentation scheme. We resolve atomic structures of portal vertex-associated tegument (PVAT) and identify multiple configurations of PVAT arising from layered reorganization of pUL77, pUL48 (large tegument protein), and pUL47 (inner tegument protein) assemblies. Analyses show that pUL77 seals the last-packaged viral genome end through electrostatic interactions, pUL77 and pUL48 harbor a head-linker-capsid-binding motif conducive to PVAT reconfiguration, and pUL47/48 dimers form 45-nm-long filaments extending from the portal vertex. These results provide a structural framework for understanding how herpesvirus tegument facilitates and evolves during processes spanning viral genome packaging to delivery.