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- EMDB-40602: cryoEM map for design HE0537, a D4 symmetric homo-oligomer design... -
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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | cryoEM map for design HE0537, a D4 symmetric homo-oligomer designed with RFdiffusion. | ||||||||||||
![]() | Refined cryoEM map for design HE0537 | ||||||||||||
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![]() | De novo / computationally designed / Dihedral / diffusion model / RFdiffusion / BIOSYNTHETIC PROTEIN | ||||||||||||
Biological species | synthetic construct (others) | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.06 Å | ||||||||||||
![]() | Courbet A / Baker D / Watson J / Juergens D / Eisenach E | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Broadly applicable protein design with RoseTTAFold Diffusion Authors: Juergens D / Watson J / Eisenach HE / Courbet A | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 56.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.3 KB 13.3 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 8.5 KB | Display | ![]() |
Images | ![]() | 21.4 KB | ||
Masks | ![]() | 64 MB | ![]() | |
Filedesc metadata | ![]() | 4 KB | ||
Others | ![]() ![]() | 59.3 MB 59.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 729.3 KB | Display | ![]() |
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Full document | ![]() | 728.8 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 21.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Refined cryoEM map for design HE0537 | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.883 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Half map A of cryoEM map for design HE0537
File | emd_40602_half_map_1.map | ||||||||||||
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Annotation | Half map A of cryoEM map for design HE0537 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map B of cryoEM map for design HE0537
File | emd_40602_half_map_2.map | ||||||||||||
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Annotation | Half map B of cryoEM map for design HE0537 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : HE0537
Entire | Name: HE0537 |
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Components |
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-Supramolecule #1: HE0537
Supramolecule | Name: HE0537 / type: complex / ID: 1 / Parent: 0 Details: De novo D4 symmetric homooligomer designed with RFdiffusion |
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Source (natural) | Organism: synthetic construct (others) |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 10 mg/mL | |||||||||
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Buffer | pH: 8.4 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK III |
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Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 1116 / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm |