EMDB-4040: Structure of mammalian respiratory Complex I, class1

Basic information

• Entry: Database: EMDB / ID: EMD-4040
• Title: Structure of mammalian respiratory Complex I, class1

Sample

• Complex: Bovine respiratory Complex I
  • Protein or peptide: x 44 types
• Ligand: x 5 types

• Keywords: NADH:ubiquinone oxidoreductase / multienzyme complexes / Complex I / mitochondria / oxidoreductase

Function / homology

Function:

Complex I biogenesis / RHOG GTPase cycle / Respiratory electron transport / cellular response to oxygen levels / mitochondrial large ribosomal subunit binding / gliogenesis / neural precursor cell proliferation / [2Fe-2S] cluster assembly / oxygen sensor activity / Neutrophil degranulation / ubiquinone binding / Mitochondrial protein degradation / NADH:ubiquinone reductase (H+-translocating) / apoptotic mitochondrial changes / NADH dehydrogenase activity / mitochondrial ATP synthesis coupled electron transport / : / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / electron transport coupled proton transport / respiratory chain complex I / acyl binding / acyl carrier activity / NADH dehydrogenase (ubiquinone) activity / ATP synthesis coupled electron transport / quinone binding / ATP metabolic process / aerobic respiration / respiratory electron transport chain / reactive oxygen species metabolic process / neurogenesis / regulation of mitochondrial membrane potential / fatty acid binding / mitochondrial membrane / mitochondrial intermembrane space / fatty acid biosynthetic process / 2 iron, 2 sulfur cluster binding / NAD binding / FMN binding / 4 iron, 4 sulfur cluster binding / response to oxidative stress / mitochondrial inner membrane / oxidoreductase activity / mitochondrial matrix / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / apoptotic process / mitochondrion / nucleoplasm / metal ion binding / cytoplasm

Domain/homology:

Complex1_LYR-like / NADH-ubiquinone oxidoreductase 1 subunit C1 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH:ubiquinone oxidoreductase, ESSS subunit / ESSS subunit of NADH:ubiquinone oxidoreductase (complex I) / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1, NDUB1 / MNLL subunit / Tim17/Tim22/Tim23/Pmp24 family / NADH-ubiquinone oxidoreductase, subunit 10 / NADH-ubiquinone oxidoreductase subunit 10 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUC2 / NADH:ubiquinone oxidoreductase, subunit NDUFB4 / NADH-ubiquinone oxidoreductase subunit b14.5b (NDUFC2) / NADH-ubiquinone oxidoreductase B15 subunit (NDUFB4) / NADH dehydrogenase 1, beta subcomplex, subunit 6 / NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit / : / NADH:ubiquinone oxidoreductase chain 4, N-terminal / NADH:ubiquinone oxidoreductase, chain 2 / NADH dehydrogenase subunit 2, C-terminal / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / : / : / NADH-ubiquinone oxidoreductase chain 4, amino terminus / NADH dehydrogenase subunit 2 C-terminus / NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase 1 alpha subcomplex subunit 3 / Zinc finger, CHCC-type / Zinc-finger domain / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase subunit 5, C-terminal / NADH dehydrogenase subunit 5 C-terminus / NADH:ubiquinone oxidoreductase, NDUFS5-15kDa / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NADH:ubiquinone oxidoreductase, iron-sulphur subunit 5 / NDUFA6, LYR domain / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / Deoxynucleoside kinase domain / Deoxynucleoside kinase / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / : / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / SLBB domain / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH-plastoquinone oxidoreductase, chain 5 subgroup / NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ / NADH-quinone oxidoreductase, chain M/4 / NADH-ubiquinone oxidoreductase chain 4L/K / NADH:ubiquinone/plastoquinone oxidoreductase, chain 6 / NADH-ubiquinone/plastoquinone oxidoreductase chain 6 / Complex 1 LYR protein domain / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminal / NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus / Complex 1 protein (LYR family) / NAD(P)H-quinone oxidoreductase subunit D/H / NADH-quinone oxidoreductase, chain 5-like / NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site / NADH-ubiquinone oxidoreductase chain 4L/Mnh complex subunit C1-like / NADH-ubiquinone/plastoquinone oxidoreductase chain 4L / Respiratory chain NADH dehydrogenase 49 Kd subunit signature. / : / NADH-quinone oxidoreductase, subunit D / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase, 49 Kd subunit / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / NADH ubiquinone oxidoreductase, F subunit / NADH dehydrogenase, subunit C / NADH:ubiquinone oxidoreductase, 30kDa subunit, conserved site / Respiratory chain NADH dehydrogenase 30 Kd subunit signature. / NADH:ubiquinone oxidoreductase / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone oxidoreductase, 30kDa subunit / NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily / Respiratory-chain NADH dehydrogenase, 30 Kd subunit / NADH:quinone oxidoreductase/Mrp antiporter, membrane subunit / Proton-conducting membrane transporter

Component:

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH-ubiquinone oxidoreductase chain 1 / NADH-ubiquinone oxidoreductase chain 2 / NADH-ubiquinone oxidoreductase chain 3 / NADH-ubiquinone oxidoreductase chain 4L / NADH-ubiquinone oxidoreductase chain 4 / NADH-ubiquinone oxidoreductase chain 5 / NADH-ubiquinone oxidoreductase chain 6 / NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial / NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 / Acyl carrier protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 subunit C2 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

• Biological species: Bos taurus (cattle)
• Method: single particle reconstruction / cryo EM / Resolution: 4.27 Å
• Authors: Vinothkumar KR / Zhu J

Funding support

United Kingdom, 1 items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	U105184322/U105663141	United Kingdom

• Citation: Journal: Nature / Year: 2016; Title: Structure of mammalian respiratory complex I.; Authors: Jiapeng Zhu / Kutti R Vinothkumar / Judy Hirst / ; Abstract: Complex I (NADH:ubiquinone oxidoreductase), one of the largest membrane-bound enzymes in the cell, powers ATP synthesis in mammalian mitochondria by using the reducing potential of NADH to drive protons across the inner mitochondrial membrane. Mammalian complex I (ref. 1) contains 45 subunits, comprising 14 core subunits that house the catalytic machinery (and are conserved from bacteria to humans) and a mammalian-specific cohort of 31 supernumerary subunits. Knowledge of the structures and functions of the supernumerary subunits is fragmentary. Here we describe a 4.2-Å resolution single-particle electron cryomicroscopy structure of complex I from Bos taurus. We have located and modelled all 45 subunits, including the 31 supernumerary subunits, to provide the entire structure of the mammalian complex. Computational sorting of the particles identified different structural classes, related by subtle domain movements, which reveal conformationally dynamic regions and match biochemical descriptions of the 'active-to-de-active' enzyme transition that occurs during hypoxia. Our structures therefore provide a foundation for understanding complex I assembly and the effects of mutations that cause clinically relevant complex I dysfunctions, give insights into the structural and functional roles of the supernumerary subunits and reveal new information on the mechanism and regulation of catalysis.

History

Deposition	Jun 28, 2016	-
Header (metadata) release	Sep 7, 2016	-
Map release	Sep 7, 2016	-
Update	Nov 20, 2024	-
Current status	Nov 20, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_4040.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.33 Å

Density

Contour Level	By AUTHOR: 0.165 / Movie #1: 0.165
Minimum - Maximum	-0.3009948 - 1.3175166
Average (Standard dev.)	0.00006958923 (±0.026574211)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 360 360 360 Spacing 360 360 360
Cell	A=B=C: 478.80002 Å α=β=γ: 90.0 °

CCP4 map header:

mode	Image stored as Reals
Å/pix. X/Y/Z	1.33	1.33	1.33
M x/y/z	360	360	360
origin x/y/z	0.000	0.000	0.000
length x/y/z	478.800	478.800	478.800
α/β/γ	90.000	90.000	90.000
start NX/NY/NZ	-147	-147	-146
NX/NY/NZ	294	294	294
MAP C/R/S	1	2	3
start NC/NR/NS	0	0	0
NC/NR/NS	360	360	360
D min/max/mean	-0.301	1.318	0.000

Supplemental data

Half map: #1

• File: emd_4040_half_map_1.map

Half map: #2

• File: emd_4040_half_map_2.map

Sample components

Entire : Bovine respiratory Complex I

• Entire: Name: Bovine respiratory Complex I

Components

• Complex: Bovine respiratory Complex I
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial,NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 6
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4L
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 5
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 4
  • Protein or peptide: NADH-ubiquinone oxidoreductase chain 2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, NDUFA10,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, NDUFA10,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, NDUFA9
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NDUFS4
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, NDUFS6,NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial,NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Protein or peptide: Acyl carrier protein, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, NDUFA8
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3, NDUFA3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, NDUFB5
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6, NDUFB6
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, NDUFB2
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, NDUFB3
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, NDUFB8
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NDUFB4,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NDUFB4,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, NDUFB9
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12
  • Protein or peptide: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NDUFA7
  • Protein or peptide: NADH dehydrogenase [ubiquinone] flavoprotein 3, NDUFV3
• Ligand: IRON/SULFUR CLUSTER
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE
• Ligand: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: ZINC ION

Supramolecule #1: Bovine respiratory Complex I

• Supramolecule: Name: Bovine respiratory Complex I / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44; Details: Complex I is the first enzyme in the electron transport chain located in the inner membrane of mitochondria and in higher eukaryotes, it is a multi-subunit membrane protein complex, consisting of >40 subunits.
• Source (natural): Organism: Bos taurus (cattle) / Organ: Heart / Organelle: Mitochondria / Location in cell: Mitochondria
• Molecular weight: Theoretical: 1 MDa

Macromolecule #1: NADH-ubiquinone oxidoreductase chain 3

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 3 / type: protein_or_peptide / ID: 1 / Details: Residues 28-50 are disordered or poor density / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 13.058521 KDa

Sequence

String:

MNLMLALLTN FTLATLLVII AFWLPQLNVY SEKTSPYECG FDPMGSARLP FSMKFFLVAI TFLLFDLEIA LLLPLPWASQ TANLNTMLT MALFLIILLA VSLAYEWTQK GLEWTE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #2: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial; type: protein_or_peptide / ID: 2 ; Details: This chain coordinates an iron-sulphur cluster (SF4); Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 20.104531 KDa

Sequence

String:

PSSTQPAVSQ ARAVVPKPAA LPSSRGEYVV AKLDDLINWA RRSSLWPMTF GLACCAVEMM HMAAPRYDMD RFGVVFRASP RQSDVMIVA GTLTNKMAPA LRKVYDQMPE PRYVVSMGSC ANGGGYYHYS YSVVRGCDRI VPVDIYVPGC PPTAEALLYG I LQLQKKIK REKRLRIWYR R

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #3: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 26.464807 KDa

Sequence

String:

ESSAADTRPT VRPRNDVAHK QLSAFGEYVA EILPKYVQQV QVSCFNELEI CIHPDGVIPV LTFLRDHSNA QFKSLADLTA VDIPTRQNR FEIVYNLLSL RFNSRIRVKT YTDELTPIES SVPVYKAANW YEREIWDMFG VFFANHPDLR RILTDYGFEG H PFRKDFPL SGYVELRYDD EVKRVVAEPV ELAQEFRKFD LNSPWEAFPA YRQPPESLKL EAGDTKPEAK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial

Macromolecule #4: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial; type: protein_or_peptide / ID: 4 ; Details: Residues 51-60 not modelled 129 is R for Cambridge cows instead of Q; Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 49.165289 KDa

Sequence

String:

RQWQPDVEWA EQYGGAVMYP TKETAHWKPP PWNDVDPPKD TLVSNLTLNF GPQHPAAHGV LRLVMELSGE MVRKCDPHIG LLHRGTEKL IEYKTYLQAL PYFDRLDYVS MMCNEQAYSL AVEKLLNIRP PPRAQWIRVL FGEITRLLNH IMAVTTHALD I GAMTPFFW MFEEREKMFE FYERVSGARM HAAYVRPGGV HQDLPLGLMD DIYEFSKNFS LRIDELEEML TNNRIWRNRT VD IGIVTAE DALNYGFSGV MLRGSGIQWD LRKTQPYDVY DQVEFDVPIG SRGDCYDRYL CRVEEMRQSI RIISQCLNKM PPG EIKVDD AKVSPPKRAE MKTSMESLIH HFKLYTEGYQ VPPGATYTAI EAPKGEFGVY LVSDGSSRPY RCKIKAPGFA HLAG LDKMS KGHMLADVVA IIGTQDIVFG EVDR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Macromolecule #5: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; type: protein_or_peptide / ID: 5 ; Details: This chain coordinates an iron-sulphur cluster (FES); Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 23.840361 KDa

Sequence

String:

GAGGALFVHR DTPENNPETP FDFTPENYKR IEAIVKNYPE GHKAAAVLPV LDLAQRQNGW LPISAMNKVA EILQVPPMRV YEVATFYTM YNRKPVGKYH IQVCTTTPCM LRNSDSILEA IQKKLGIKVG ETTPDKLFTL IEVECLGACV NAPMVQINDN Y YEDLTPKD IEEIIDELKA GKIPKPGPRS GRFSCEPAGG LTSLTEPPKG PGFGVQAGL

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial

Macromolecule #6: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; type: protein_or_peptide / ID: 6 / Details: This chain coordinates SF4 and also binds FMN / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 48.562137 KDa

Sequence

String:

SGDTTAPKKT SFGSLKDEDR IFTNLYGRHD WRLKGAQSRG DWYKTKEILL KGPDWILGEV KTSGLRGRGG AGFPTGLKWS FMNKPSDGR PKYLVVNADE GEPGTCKDRE IIRHDPHKLV EGCLVGGRAM GARAAYIYIR GEFYNEASNL QVAIREAYEA G LIGKNACG SGYDFDVFVV RGAGAYICGE ETALIESIEG KQGKPRLKPP FPADVGVFGC PTTVANVETV AVSPTICRRG GA WFASFGR ERNSGTKLFN ISGHVNNPCT VEEEMSVPLK ELIEKHAGGV TGGWDNLLAV IPGGSSTPLI PKSVCETVLM DFD ALIQAQ TGLGTAAVIV MDRSTDIVKA IARLIEFYKH ESCGQCTPCR EGVDWMNKVM ARFVRGDARP AEIDSLWEIS KQIE GHTIC ALGDGAAWPV QGLIRHFRPE LEERMQQFAQ QHQARQAAF

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Macromolecule #7: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial,NADH...

• Macromolecule: Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial,NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; type: protein_or_peptide / ID: 7 ; Details: This chain coordinates 2xSF4 and 1FES. We are uncertain of the register 210-692; Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 71.847797 KDa

Sequence

String:

LIEVFVDGQS VMVEPGTTVL QACEKVGMQI PRFCYHERLS VAGNCRMCLV EIEKAPKVVA ACAMPVMKGW NILTNSEKTK KAREGVMEF LLANHPLDCP ICDQGGECDL QDQSMMFGSD RSRFLEGKRA VEDKNIGPLV KTIMTRCIQC TRCIRFASEI A GVDDLGTT GRGNDMQVGT YIEKMFMSEL SGNIIDICPV GALT(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

UniProtKB: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Macromolecule #8: NADH-ubiquinone oxidoreductase chain 1

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 1 / type: protein_or_peptide / ID: 8 / Details: Residues 201-217 are disordered / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 35.688773 KDa

Sequence

String:

MFMINILMLI IPILLAVAFL TLVERKVLGY MQLRKGPNVV GPYGLLQPIA DAIKLFIKEP LRPATSSASM FILAPIMALG LALTMWIPL PMPYPLINMN LGVLFMLAMS SLAVYSILWS GWASNSKYAL IGALRAVAQT ISYEVTLAII LLSVLLMSGS F TLSTLITT QEQMWLILPA WPLAMMWFIS TLAETNRAPF DLTEGESELV SGFNVEYAAG PFALFFMAEY ANIIMMNIFT AI LFLGTSH NPHMPELYTI NFTIKSLLLT MSFLWIRASY PRFRYDQLMH LLWKNFLPLT LALCMWHVSL PILTSGIPPQ T

UniProtKB: NADH-ubiquinone oxidoreductase chain 1

Macromolecule #9: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; type: protein_or_peptide / ID: 9 ; Details: This chain coordinates 2 iron-sulphur clusters (SF4); Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 20.219947 KDa

Sequence

String:

TYKYVNLREP SMDMKSVTDR AAQTLLWTEL IRGLGMTLSY LFREPATINY PFEKGPLSPR FRGEHALRRY PSGEERCIAC KLCEAVCPA QAITIEAEPR ADGSRRTTRY DIDMTKCIYC GFCQEACPVD AIVEGPNFEF STETHEELLY NKEKLLNNGD K WEAEIAAN IQADYLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial

Macromolecule #10: NADH-ubiquinone oxidoreductase chain 6

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 19.082479 KDa

Sequence

String:

MMLYIVFILS VIFVMGFVGF SSKPSPIYGG LGLIVSGGVG CGIVLNFGGS FLGLMVFLIY LGGMMVVFGY TTAMATEQYP EIWLSNKAV LGAFVTGLLM EFFMVYYVLK DKEVEVVFEF NGLGDWVIYD TGDSGFFSEE AMGIAALYSY GTWLVIVTGW S LLIGVVVI MEITRGN

UniProtKB: NADH-ubiquinone oxidoreductase chain 6

Macromolecule #11: NADH-ubiquinone oxidoreductase chain 4L

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4L / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 10.800186 KDa

Sequence

String:

MSMVYMNIMM AFTVSLVGLL MYRSHLMSSL LCLEGMMLSL FVMAALTILN SHFTLASMMP IILLVFAACE AALGLSLLVM VSNTYGTDY VQNLNLLQC

UniProtKB: NADH-ubiquinone oxidoreductase chain 4L

Macromolecule #12: NADH-ubiquinone oxidoreductase chain 5

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 5 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 68.327922 KDa

Sequence

String:

MNMFSSLSLV TLLLLTMPIM MMSFNTYKPS NYPLYVKTAI SYAFITSMIP TMMFIHSGQE LIISNWHWLT IQTLKLSLSF KMDYFSMMF IPVALFVTWS IMEFSMWYMY SDPNINKFFK YLLLFLITML ILVTANNLFQ LFIGWEGVGI MSFLLIGWWY G RADANTAA LQAILYNRIG DIGFILAMAW FLTNLNTWDL QQIFMLNPSD SNMPLIGLAL AATGKSAQFG LHPWLPSAME GP TPVSALL HSSTMVVAGI FLLIRFYPLT ENNKYIQSIT LCLGAITTLF TAMCALTQND IKKIIAFSTS SQLGLMMVTI GIN QPYLAF LHICTHAFFK AMLFMCSGSI IHSLNDEQDI RKMGGLFKAM PFTTTALIVG SLALTGMPFL TGFYSKDLII EAAN TSYTN AWALLMTLIA TSFTAIYSTR IIFFALLGQP RFPTLVNINE NNPLLINSIK RLLIGSLFAG YIISNNIPPT TIPQM TMPY YLKTTALIVT ILGFILALEI SNMTKNLKYH YPSNAFKFST LLGYFPTIMH RLAPYMNLSM SQKSASSLLD LIWLEA ILP KTISLAQMKA STLVTNQKGL IKLYFLSFLI TILISMILFN FHE

UniProtKB: NADH-ubiquinone oxidoreductase chain 5

Macromolecule #13: NADH-ubiquinone oxidoreductase chain 4

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 4 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 52.13075 KDa

Sequence

String:

MLKYIIPTIM LMPLTWLSKN NMIWVNSTAH SLLISFTSLL LMNQFGDNSL NFSLLFFSDS LSTPLLILTM WLLPLMLMAS QHHLSKENL TRKKLFITML ISLQLFLIMT FTAMELILFY ILFEATLVPT LIIITRWGNQ TERLNAGLYF LFYTLAGSLP L LVALIYIQ NTVGSLNFLM LQYWVQPVHN SWSNVFMWLA CMMAFMVKMP LYGLHLWLPK AHVEAPIAGS MVLAAVLLKL GG YGMLRIT LILNPMTDFM AYPFIMLSLW GMIMTSSICL RQTDLKSLIA YSSVSHMALV IVAILIQTPW SYMGATALMI AHG LTSSML FCLANSNYER IHSRTMILAR GLQTLLPLMA TWWLLASLTN LALPPTINLI GELFVVMSTF SWSNITIILM GVNM VITAL YSLYMLIMTQ RGKYTYHINN ISPSFTRENA LMSLHILPLL LLTLNPKIIL GPLY

UniProtKB: NADH-ubiquinone oxidoreductase chain 4

Macromolecule #14: NADH-ubiquinone oxidoreductase chain 2

• Macromolecule: Name: NADH-ubiquinone oxidoreductase chain 2 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO / EC number: NADH:ubiquinone reductase (H+-translocating)
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 39.27493 KDa

Sequence

String:

MNPIIFIIIL LTIMLGTIIV MISSHWLLVW IGFEMNMLAI IPIMMKNHNP RATEASTKYF LTQSTASMLL MMAVIINLMF SGQWTVMKL FNPMASMLMT MALAMKLGMA PFHFWVPEVT QGIPLSSGLI LLTWQKLAPM SVLYQIFPSI NLNLILTLSV L SILIGGWG GLNQTQLRKI MAYSSIAHMG WMTAVLPYNP TMTLLNLIIY IIMTSTMFTM FMANSTTTTL SLSHTWNKTP IM TVLILAT LLSMGGLPPL SGFMPKWMII QEMTKNNSII LPTFMAITAL LNLYFYMRLT YSTTLTMFPS TNNMKMKWQF PLM KKMTFL PTMVVLSTMM LPLTPMLSVL E

UniProtKB: NADH-ubiquinone oxidoreductase chain 2

Macromolecule #15: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, ND...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, NDUFA10,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, NDUFA10,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; type: protein_or_peptide / ID: 15 / Details: We are uncertain of the register 5-90 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 34.256562 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)GNSYRLQAW LYASRLLQYA DALEHLLSTG QGVVLERSIY SDFVFLEAMY R QGFIRKQC VDHYNQVKKV TICEYLPPHV VVYVDVPVPE VQSRIQKKGN PHEMKITSAY LQDIENAYKG TFLPEMSEKC EV LQYSAWE AQDAEKVVED IEYLKYDKGP WLDQNDRNLH KLRMLVQDKL EVLNYTSIPV FLPEVTVGAH QSDQVFQEFT ELP GRKYRA GYNEDVGDKW IWLK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial

Macromolecule #16: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, NDUFA9

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, NDUFA9; type: protein_or_peptide / ID: 16 ; Details: Residue range 187-199, 253-279 not modelled and none of the residues are assigned as the density doesn't allow it. The density for NAP is prominent and has been modelled.; Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 25.54935 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #17: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NDUFS4

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, NDUFS4; type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 9.634868 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

Macromolecule #18: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, NDUFS6,NAD...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, NDUFS6,NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial,NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial; type: protein_or_peptide / ID: 18 / Details: This chain coordinates a zinc atom / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 8.316853 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)CDG GGGALGHPRV YINLDKETKT GTCGYCGLQF RQ

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #19: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 11.097824 KDa

Sequence

String:

MAAAAAIRGV RGKLGLREIR IHLCQRSPGS QGVRDFIEKR YVELKKANPD LPILIRECSD VQPKLWARYA FGQEKNVSLN NFSADQVTR ALENVLSSKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #20: Acyl carrier protein, mitochondrial

• Macromolecule: Name: Acyl carrier protein, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 10.119541 KDa

Sequence

String:

SDAPPLTLEG IKDRVLYVLK LYDKIDPEKL SVNSHFMKDL GLDSLDQVEI IMAMEDEFGF EIPDIDAEKL MCPQEIVDYI ADKKDVYE

UniProtKB: Acyl carrier protein, mitochondrial

Macromolecule #21: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 13.203414 KDa

Sequence

String:

AGLLKKTTGL VGLAVCETPH ERLKILYTKI LDVLGHIPKN AAYRKYTEQI TNEKLSMVKA EPDVKKLEER LQGGQIEEVI LQAENELSL ARKMIQWKPW EPLVEEPPAS QWKWPI

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Macromolecule #22: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6; type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 15.083544 KDa

Sequence

String:

MAASGLRQAA VAASTSVKPI FSRDMNEAKR RVRELYRAWY REVPNTVHLF QLDISVKQGR DKVREMFKKN AHITDPRVVD LLVIKGKME LEETIKVWKQ RTHVMRFFHE TEAPRPKDFL SKFYVGHDP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #23: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8,NADH...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, NDUFA8; type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 18.115223 KDa

Sequence

String:

MPGIVELPSL EDLKVQEVKV SSSVLKAAAH HYGAQCDKPN KEFMLCRWEE KDPRRCLEEG KLVNQCALEF FRQIKRHCAE PFTEYWTCI DYSGLQLFRR CRKQQAQFDE CVLDK(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8

Macromolecule #24: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11; type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 14.772021 KDa

Sequence

String:

MAKTVLRQYW DIPEGTECHR KTYATTSIGG AAGLVVSAYS VALKTPTSFL EGVARTGRYT FTAAAIGAIF GLTSCISAQV REKPDDPLN YLIGGCAGGL ILGARTRSYG IGAAACAYMG LTAALVKMGQ LEGWQVFAEP KV

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11

Macromolecule #25: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, ND...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, NDUFA13; type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 14.1359 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)GYS MFAVGIGALL FGYWSMMKWN RERRRLQ IE DFEARIALMP LLQAEKDRRV LQMLRENLEE EATVMKD(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #26: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 8.117445 KDa

Sequence

String:

MWFEVLPGIA VMGVCLFIPG MATARIHRFS NGGKEKRVAH YPYQWYLMER DRRVSGVNRS YVSKGLENID

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #27: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3,NADH...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3,NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3, NDUFA3; type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 7.860488 KDa

Sequence

String:

AERVAAFLKN VWAKEPVLVA SFAIAGLAVI LPTLSPYTKY SLMIN(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3

Macromolecule #28: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 5.836698 KDa

Sequence

String:

KFYIQEPPHG SPNWLKVGLT LGTSVFLWIY LIKQHNEDVL EYKRRNGLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial

Macromolecule #29: NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydro...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2,NADH dehydrogenase [ubiquinone] 1 subunit C2,NADH dehydrogenase [ubiquinone] 1 subunit C2, NDUFC2; type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 11.9883 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)PRL AFVGFLGYCS GLIDNAIRRR PVLLAGL HR QLLYITSFVF VGYYLLKRQD YMYAVRDHDM FSYIKSH(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 subunit C2

Macromolecule #30: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 12.563475 KDa

Sequence

String:

PFFDVQKRLG VDLDRWMTIQ SAEQPHKIPS RCHAFEKEWI ECAHGIGSIR AEKECKIEFE DFRECLLRQK TMKRLHAIRR QREKLIKEG KYTPPPHHSG QEEPRS

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Macromolecule #31: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1; type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 6.978148 KDa

Sequence

String:

MNLLQVVRDH WVHVLVPMGF VFGYYLDRKN DEKLTAFRNK SLLYKRELKP NEEVTWK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1

Macromolecule #32: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mit...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial; type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 14.469059 KDa

Sequence

String:

ESSSSRAVIA PSTLAGKRPS EPTLRWQEDP EPEDENLYEK NPDSHGYDKD PAVDIWNMRV VFFFGFSIVL VLGSTFVAYL PDYRMQEWA RREAERLVKY REAHGLPIME SNCFDPSKIQ LPEDED

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

Macromolecule #33: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mito...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, NDUFB5; type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 12.614555 KDa

Sequence

String:

RLFIIKPSGF YDKRFLKLLR FYILLTGIPV AIGITLINV(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Macromolecule #34: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6,NADH ...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6, NDUFB6; type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 10.234333 KDa

Sequence

String:

EEKLRLQQLR ELRRRWLKDQ ELSPREP(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6

Macromolecule #35: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, NDUFB2

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, NDUFB2; type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 4.443468 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

Macromolecule #36: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, NDUFB3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, NDUFB3; type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 6.315777 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #37: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, NDUFB8

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, NDUFB8; type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 10.060393 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

Macromolecule #38: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NDUF...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NDUFB4,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4, NDUFB4,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 13.415493 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)QAERLAIR SRLKREYQLQ YYDPSRRGVI EDPALVRWTY ARSANIYPNF RPNTKTS LL GALFGIGPLV FWYYVFKTDR DRKEKLIQEG KLDRTFNISY

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4

Macromolecule #39: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH ...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9, NDUFB9; type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 19.246094 KDa

Sequence

String:

THQQKVLRLY KRALRHLESW CIHRDKYRYF ACLLRARFDE HKNEKDMVKA TQLLREAEEE FWHGQHPQPY IFPESPGGTS YERYECYKV PEWCLDDWHP SEKAMYPDYF AKREQWKKLR RESWEREV(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #40: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 16.297649 KDa

Sequence

String:

GAHLARRYLG DASVEPDPLR MPTFPPDYGF PERKEREMVA TQQEMNDAQL VLQQRDYCAH YLIRFLKCKR DSFPNFLACK HERHDWDYC EHLDYVKRMK EFERERRLLQ RKKRREQREA DMAKGLGPGE VAPEVAL

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #41: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDU...

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10,NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, NDUFB10; type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 16.906949 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)CQEK DVLCMFEAEM QWRRDYKVDQ EIVNIIQERL KACQQR EGE SHRQNCAKEL EQFTQVVKAY QDRY(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Macromolecule #42: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12; type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 17.115508 KDa

Sequence

String:

MELLQVLKRG LQQVSGHGGL RGYLRVLFRA NDVRVGTLVG EDKYGNKYYE DNKQFFGRHR WVIYTTEMNG KNTFWDVDGS MVPPEWHRW LHCMTDDPPT VKPPTARKFI WTNHKFNLSG TPQQYVPYST TRKKIQEWVP PSTPYK

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Macromolecule #43: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NDUFA7

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7, NDUFA7; type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 7.42214 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

Macromolecule #44: NADH dehydrogenase [ubiquinone] flavoprotein 3, NDUFV3

• Macromolecule: Name: NADH dehydrogenase [ubiquinone] flavoprotein 3, NDUFV3; type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Bos taurus (cattle)
• Molecular weight: Theoretical: 2.996685 KDa

Sequence

String:

(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

Macromolecule #45: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 45 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #46: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 46 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #47: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 47 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Macromolecule #48: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / type: ligand / ID: 48 / Number of copies: 1 / Formula: NAP
• Molecular weight: Theoretical: 743.405 Da

Chemical component information

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #49: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 49 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 3.5 mg/mL
• Buffer: pH: 8 / Component - Concentration: 150.0 mM / Component - Formula: NaCl / Component - Name: sodium chloride / Details: 20 mM TRIS-HCL, 150 mM NaCl, 0.03% CYMAL-7
• Grid: Model: Quantifoil / Material: GOLD / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 75 sec. / Pretreatment - Atmosphere: AIR; Details: Holey carbon grids were placed on a filter paper in a glass petridish and pumped for around 5 minutes to remove any residual moisture. The glow of the plasma was observed and glow discharge when it was purple.
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: HOMEMADE PLUNGER; Details: The specimen was vitrified in an environmental plunge-freeze apparatus present in a cold room, blot for 12-15 seconds after the diameter of the blotted meniscus ceases to spread and plunged..
• Details: Enzyme was purified from bovine heart mitochondria. The detergent used for the final step is cymal-7.

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Temperature: Min: 87.5 K / Max: 87.5 K
• Image recording: Film or detector model: FEI FALCON II (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Digitization - Frames/image: 1-34 / Average exposure time: 2.0 sec. / Average electron dose: 35.0 e/Ų; Details: Images were collected in movie mode at 17 frames per second. Thus each frame has ~1.0 e/A2
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 70.0 µm / Calibrated defocus max: 5.5 µm / Calibrated defocus min: 1.8 µm / Calibrated magnification: 105263 / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal magnification: 59000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Details: Each image was exposed for 2 seconds resulting in a total dose of ~35 e/A2s. The frames were captures with a in-house protocol at 17 frames/second.
• Particle selection: Number selected: 139456 / Details: All particles were picked manually

Startup model

Type of model: EMDB MAP
EMDB ID:

2676

Details: The map of bovine complex I was low pass filtered to 60A before refinement

• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.27 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Software - details: Maximum Likelihood / Number images used: 48033
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: OTHER / Software - Name: RELION (ver. 1.4) / Software - details: Maximum Likelihood
• Final 3D classification: Number classes: 8 / Software - Name: RELION (ver. 1.4) / Software - details: Maximum Likelihood; Details: The data set with particles after 2D classification had 115,966 particles. After the angular assignment, movie refinement, B-factor weighting and refinement, the particles were subjected to 3D classification with number of classes set to 8. Three classes were identified to contain high-resolution features and the map deposited with this is one of the classes.

Atomic model buiding 1

• Refinement: Space: RECIPROCAL / Protocol: OTHER / Overall B value: 83.049 / Target criteria: Maximum likelihood

Output model

PDB-5ldw:
Structure of mammalian respiratory Complex I, class1