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- EMDB-39462: Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of M... -

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Basic information

Entry
Database: EMDB / ID: EMD-39462
TitleCryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis
Map dataMycobacterium smegmatis MetAP1c-30S subunit complex
Sample
  • Complex: Mycobacterium smegmatis 30S subunit in complex with methionine aminopeptidase type 1c
    • Organelle or cellular component: Methionine aminopeptidase Type1c protein
      • Protein or peptide: x 1 types
    • RNA: x 1 types
    • Protein or peptide: x 18 types
KeywordsMethionine aminopeptidase type1c / anti-association factor / inter-subunit face / RIBOSOME
Function / homology
Function and homology information


methionyl aminopeptidase / initiator methionyl aminopeptidase activity / metalloaminopeptidase activity / transition metal ion binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / small ribosomal subunit rRNA binding / tRNA binding ...methionyl aminopeptidase / initiator methionyl aminopeptidase activity / metalloaminopeptidase activity / transition metal ion binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / small ribosomal subunit rRNA binding / tRNA binding / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / proteolysis / RNA binding / zinc ion binding / cytosol / cytoplasm
Similarity search - Function
Methionine aminopeptidase subfamily 1 signature. / Peptidase M24A, methionine aminopeptidase, subfamily 1 / Peptidase M24, methionine aminopeptidase / Peptidase M24 / Metallopeptidase family M24 / Creatinase/aminopeptidase-like / Ribosomal protein S14, type Z / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S19, bacterial-type ...Methionine aminopeptidase subfamily 1 signature. / Peptidase M24A, methionine aminopeptidase, subfamily 1 / Peptidase M24, methionine aminopeptidase / Peptidase M24 / Metallopeptidase family M24 / Creatinase/aminopeptidase-like / Ribosomal protein S14, type Z / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S11, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S4, bacterial-type / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein S14/S29 / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S12, bacterial-type / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / K Homology domain / K homology RNA-binding domain / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / Ribosomal protein S10, conserved site / Ribosomal protein S10 signature. / Ribosomal protein S14, conserved site / Ribosomal protein S14 signature. / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal / Ribosomal protein S3, C-terminal domain / Ribosomal protein S3, C-terminal domain superfamily / Ribosomal protein S15/S19, conserved site / Ribosomal protein S19 signature. / Ribosomal protein S10 / Ribosomal protein S19/S15 / Ribosomal protein S19/S15, superfamily / Ribosomal protein S19 / : / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S7, conserved site / Ribosomal protein S7 signature. / Ribosomal protein S17, conserved site / Ribosomal protein S17 signature. / K homology domain superfamily, prokaryotic type / Ribosomal protein S5 / Ribosomal protein S13, conserved site / Ribosomal protein S13 signature. / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S13 / 30s ribosomal protein S13, C-terminal / Ribosomal protein S13/S18 / Ribosomal protein S5, C-terminal / Ribosomal protein S13 family profile. / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein S8 signature. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4, conserved site / Ribosomal protein S15 signature. / Ribosomal protein S4 signature. / Ribosomal protein S4/S9 N-terminal domain / Ribosomal protein S4/S9, N-terminal / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S4/S9 / K homology domain-like, alpha/beta / Ribosomal protein S8 / Ribosomal protein S8 superfamily / Ribosomal protein S8 / S4 RNA-binding domain profile. / Ribosomal protein S13-like, H2TH / Ribosomal S11, conserved site / Ribosomal protein S11 signature. / S4 RNA-binding domain / S4 domain / RNA-binding S4 domain
Similarity search - Domain/homology
Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS5 ...Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS14B / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS9 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein bS18B / Methionine aminopeptidase
Similarity search - Component
Biological speciesMycolicibacterium smegmatis MC2 155 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.7 Å
AuthorsBanerjee A / Srinivasan K / Sengupta J
Funding support India, 3 items
OrganizationGrant numberCountry
Science and Engineering Research Board (SERB)SPF/2021/000141 India
Science and Engineering Research Board (SERB)CRG/2019/001788 India
Council of Scientific & Industrial Research (CSIR)MLP-139 India
CitationJournal: To Be Published
Title: Cryo-EM map of 30S ribosomal subunit in complex with MetAP1c of Mycobacterium smegmatis
Authors: Banerjee A / Srinivasan K / Sengupta J
History
DepositionMar 15, 2024-
Header (metadata) releaseMar 19, 2025-
Map releaseMar 19, 2025-
UpdateMar 19, 2025-
Current statusMar 19, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_39462.png

Downloads & links

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Map

FileDownload / File: emd_39462.map.gz / Format: CCP4 / Size: 163.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMycobacterium smegmatis MetAP1c-30S subunit complex
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 350 pix.
= 374.5 Å		1.07 Å/pix.
x 350 pix.
= 374.5 Å		1.07 Å/pix.
x 350 pix.
= 374.5 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.005
Minimum - Maximum-0.00917657 - 0.067586705
Average (Standard dev.)0.00016011044 (±0.0031258774)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions350350350
Spacing350350350
CellA=B=C: 374.50003 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Mycobacterium smegmatis MetAP1c-30S subunit complex

Fileemd_39462_half_map_1.map
AnnotationMycobacterium smegmatis MetAP1c-30S subunit complex
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Mycobacterium smegmatis MetAP1c-30S subunit complex

Fileemd_39462_half_map_2.map
AnnotationMycobacterium smegmatis MetAP1c-30S subunit complex
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Mycobacterium smegmatis 30S subunit in complex with methionine am...

EntireName: Mycobacterium smegmatis 30S subunit in complex with methionine aminopeptidase type 1c
Components
  • Complex: Mycobacterium smegmatis 30S subunit in complex with methionine aminopeptidase type 1c
    • Organelle or cellular component: Methionine aminopeptidase Type1c protein
      • Protein or peptide: Methionine aminopeptidase
    • RNA: 16S rRNA
    • Protein or peptide: Small ribosomal subunit protein uS3
    • Protein or peptide: Small ribosomal subunit protein uS4
    • Protein or peptide: Small ribosomal subunit protein uS5
    • Protein or peptide: Small ribosomal subunit protein bS6
    • Protein or peptide: 30S ribosomal protein S7
    • Protein or peptide: Small ribosomal subunit protein uS8
    • Protein or peptide: Small ribosomal subunit protein uS9
    • Protein or peptide: Small ribosomal subunit protein uS10
    • Protein or peptide: Small ribosomal subunit protein uS11
    • Protein or peptide: Small ribosomal subunit protein uS12
    • Protein or peptide: Small ribosomal subunit protein uS14B
    • Protein or peptide: Small ribosomal subunit protein uS15
    • Protein or peptide: Small ribosomal subunit protein bS16
    • Protein or peptide: Small ribosomal subunit protein uS17
    • Protein or peptide: Small ribosomal subunit protein bS18B
    • Protein or peptide: Small ribosomal subunit protein bS20
    • Protein or peptide: Small ribosomal subunit protein uS19
    • Protein or peptide: Small ribosomal subunit protein uS13

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Supramolecule #1: Mycobacterium smegmatis 30S subunit in complex with methionine am...

SupramoleculeName: Mycobacterium smegmatis 30S subunit in complex with methionine aminopeptidase type 1c
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)

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Supramolecule #2: Methionine aminopeptidase Type1c protein

SupramoleculeName: Methionine aminopeptidase Type1c protein / type: organelle_or_cellular_component / ID: 2 / Parent: 1 / Macromolecule list: #19
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)

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Macromolecule #1: 16S rRNA

MacromoleculeName: 16S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 489.828562 KDa
SequenceString: UGGAGAGUUU GAUCCUGGCU CAGGACGAAC GCUGGCGGCG UGCUUAACAC AUGCAAGUCG AACGGAAAGG CCCUUUCGGG GGUACUCGA GUGGCGAACG GGUGAGUAAC ACGUGGGUGA UCUGCCCUGC ACUUUGGGAU AAGCCUGGGA AACUGGGUCU A AUACCGAA ...String:
UGGAGAGUUU GAUCCUGGCU CAGGACGAAC GCUGGCGGCG UGCUUAACAC AUGCAAGUCG AACGGAAAGG CCCUUUCGGG GGUACUCGA GUGGCGAACG GGUGAGUAAC ACGUGGGUGA UCUGCCCUGC ACUUUGGGAU AAGCCUGGGA AACUGGGUCU A AUACCGAA UACACCCUGC UGGUCGCAUG GCCUGGUAGG GGAAAGCUUU UGCGGUGUGG GAUGGGCCCG CGGCCUAUCA GC UUGUUGG UGGGGUGAUG GCCUACCAAG GCGACGACGG GUAGCCGGCC UGAGAGGGUG ACCGGCCACA CUGGGACUGA GAU ACGGCC CAGACUCCUA CGGGAGGCAG CAGUGGGGAA UAUUGCACAA UGGGCGCAAG CCUGAUGCAG CGACGCCGCG UGAG GGAUG ACGGCCUUCG GGUUGUAAAC CUCUUUCAGC ACAGACGAAG CGCAAGUGAC GGUAUGUGCA GAAGAAGGAC CGGCC AACU ACGUGCCAGC AGCCGCGGUA AUACGUAGGG UCCGAGCGUU GUCCGGAAUU ACUGGGCGUA AAGAGCUCGU AGGUGG UUU GUCGCGUUGU UCGUGAAAAC UCACAGCUUA ACUGUGGGCG UGCGGGCGAU ACGGGCAGAC UAGAGUACUG CAGGGGA GA CUGGAAUUCC UGGUGUAGCG GUGGAAUGCG CAGAUAUCAG GAGGAACACC GGUGGCGAAG GCGGGUCUCU GGGCAGUA A CUGACGCUGA GGAGCGAAAG CGUGGGGAGC GAACAGGAUU AGAUACCCUG GUAGUCCACG CCGUAAACGG UGGGUACUA GGUGUGGGUU UCCUUCCUUG GGAUCCGUGC CGUAGCUAAC GCAUUAAGUA CCCCGCCUGG GGAGUACGGC CGCAAGGCUA AAACUCAAA GGAAUUGACG GGGGCCCGCA CAAGCGGCGG AGCAUGUGGA UUAAUUCGAU GCAACGCGAA GAACCUUACC U GGGUUUGA CAUGCACAGG ACGCCGGCAG AGAUGUCGGU UCCCUUGUGG CCUGUGUGCA GGUGGUGCAU GGCUGUCGUC AG CUCGUGU CGUGAGAUGU UGGGUUAAGU CCCGCAACGA GCGCAACCCU UGUCUCAUGU UGCCAGCACG UUAUGGUGGG GAC UCGUGA GAGACUGCCG GGGUCAACUC GGAGGAAGGU GGGGAUGACG UCAAGUCAUC AUGCCCCUUA UGUCCAGGGC UUCA CACAU GCUACAAUGG CCGGUACAAA GGGCUGCGAU GCCGUGAGGU GGAGCGAAUC CUUUCAAAGC CGGUCUCAGU UCGGA UCGG GGUCUGCAAC UCGACCCCGU GAAGUCGGAG UCGCUAGUAA UCGCAGAUCA GCAACGCUGC GGUGAAUACG UUCCCG GGC CUUGUACACA CCGCCCGUCA CGUCAUGAAA GUCGGUAACA CCCGAAGCCG GUGGCCUAAC CCUUGUGGAG GGAGCCG UC GAAGGUGGGA UCGGCGAUUG GGACGAAGUC GUAACAAGGU AGCCGUACCG GAAGGUGCGG CUGGAUCA

GENBANK: GENBANK: CP000480.1

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Macromolecule #2: Small ribosomal subunit protein uS3

MacromoleculeName: Small ribosomal subunit protein uS3 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.805396 KDa
SequenceString: MGQKINPHGF RLGITTEWKS RWYADKQYKD YVKEDVAIRK LLATGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY ...String:
MGQKINPHGF RLGITTEWKS RWYADKQYKD YVKEDVAIRK LLATGLERAG IADVEIERTR DRVRVDIHTA RPGIVIGRRG TEADRIRAD LEKLTGKQVQ LNILEVKNPE SQAQLVAQGV AEQLSNRVAF RRAMRKAIQS AMRQPNVKGI RVQCSGRLGG A EMSRSEFY REGRVPLHTL RADIDYGLYE AKTTFGRIGV KVWIYKGDIV GG

UniProtKB: Small ribosomal subunit protein uS3

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Macromolecule #3: Small ribosomal subunit protein uS4

MacromoleculeName: Small ribosomal subunit protein uS4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 23.28459 KDa
SequenceString: ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV ...String:
ARYTGPATRK SRRLGVDLVG GDQSFEKRPY PPGQHGRARI KESEYRQQLQ EKQKARFSYG VMEKQFRRYY EEANRQPGKT GDNLLRILE SRLDNVVYRA GLARTRRMAR QLVSHGHFLV NGVKVDIPSY RVSQYDIIDV KEKSLNTLPF QIARETAGER P IPSWLQVV GERQRILVHQ LPERAQIDVP LTEQLIVELY SK

UniProtKB: Small ribosomal subunit protein uS4

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Macromolecule #4: Small ribosomal subunit protein uS5

MacromoleculeName: Small ribosomal subunit protein uS5 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 20.443531 KDa
SequenceString: RGRRDDRGGR GRDGGDKSNY IERVVSINRV SKVVKGGRRF SFTALVIVGD GKGMVGVGYG KAKEVPAAIA KGVEEARKNF FRVPLIGST ITHPVQGEAA AGVVMLRPAS PGTGVIAGGA ARAVLECAGV HDILAKSLGS DNAINVVHAT VAALKLLQRP E EVAARRGL ...String:
RGRRDDRGGR GRDGGDKSNY IERVVSINRV SKVVKGGRRF SFTALVIVGD GKGMVGVGYG KAKEVPAAIA KGVEEARKNF FRVPLIGST ITHPVQGEAA AGVVMLRPAS PGTGVIAGGA ARAVLECAGV HDILAKSLGS DNAINVVHAT VAALKLLQRP E EVAARRGL PIEDVAPAGM LKARRESEAL AAAAAREGSA

UniProtKB: Small ribosomal subunit protein uS5

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Macromolecule #5: Small ribosomal subunit protein bS6

MacromoleculeName: Small ribosomal subunit protein bS6 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.991637 KDa
SequenceString:
MRPYEIMVIL DPTLDERTVA PSLETFLNVI RKDGGTVDKV DIWGRRRLAY EIAKHAEGIY AVIDVKAEPA TVSELDRQLN LNESVLRTK VLRTDKH

UniProtKB: Small ribosomal subunit protein bS6

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Macromolecule #6: 30S ribosomal protein S7

MacromoleculeName: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 17.660375 KDa
SequenceString:
MPRKGPAPKR PLVNDPVYGS QLVTQLVNKV LLEGKKSLAE RIVYGALEQA REKTGTDPVV TLKRALDNVK PALEVRSRRV GGATYQVPV EVRPDRSTTL ALRWLVNFSR QRREKTMVER LANEILDASN GLGASVKRRE DTHKMAEANR AFAHYRW

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #7: Small ribosomal subunit protein uS8

MacromoleculeName: Small ribosomal subunit protein uS8 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.26034 KDa
SequenceString:
MTDPIADFLT RLRNANSAYH DEVTLPHSKL KANIAEILKR EGYISDYRTE DARVGKSLVV QLKYGPSRER SIAGLRRVSK PGLRVYAKS TNLPRVLGGL GVAIISTSSG LLTDRQAARQ GVGGEVLAYV W

UniProtKB: Small ribosomal subunit protein uS8

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Macromolecule #8: Small ribosomal subunit protein uS9

MacromoleculeName: Small ribosomal subunit protein uS9 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 14.093392 KDa
SequenceString:
PIQTVGRRKE AVVRVRLVPG TGQFNLDGRT LENYFPNKVH QQLIKAPLVT VDRVDQFDIY AHLDGGGPSG QAGALRLAIA RALILVQPE DRPALKKAGF LTRDPRAIER KKYGLKKARK APQYSKR

UniProtKB: Small ribosomal subunit protein uS9

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Macromolecule #9: Small ribosomal subunit protein uS10

MacromoleculeName: Small ribosomal subunit protein uS10 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 11.066859 KDa
SequenceString:
KIRIRLKAYD HEAIDASARK IVETVTRTGA SVVGPVPLPT EKNVYCVIRS PHKYKDSREH FEMRTHKRLI DILDPTPKTV DALMRIDLP ASVDVNIQ

UniProtKB: Small ribosomal subunit protein uS10

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Macromolecule #10: Small ribosomal subunit protein uS11

MacromoleculeName: Small ribosomal subunit protein uS11 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.38004 KDa
SequenceString:
KKNVPHGAAH IKSTFNNTIV SITDPQGNVI AWASSGHVGF KGSRKSTPFA AQLAAENAAR KAQEHGVKKV DVFVKGPGSG RETAIRSLQ AAGLEVGTIS DVTPQPHNGC RPPKRRRV

UniProtKB: Small ribosomal subunit protein uS11

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Macromolecule #11: Small ribosomal subunit protein uS12

MacromoleculeName: Small ribosomal subunit protein uS12 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.678093 KDa
SequenceString:
PTIQQLVRKG RRDKIAKVKT AALKGSPQRR GVCTRVYTTT PKKPNSALRK VARVKLTSQV EVTAYIPGEG HNLQEHSMVL VRGGRVKDL PGVRYKIIRG SLDTQGVKNR KQARSRYGAK KEK

UniProtKB: Small ribosomal subunit protein uS12

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Macromolecule #12: Small ribosomal subunit protein uS14B

MacromoleculeName: Small ribosomal subunit protein uS14B / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 6.845213 KDa
SequenceString:
AKKALVHKAN KKPKFAVRAY TRCNKCGRPH SVYRKFGLCR ICLREMAHAG ELPGVQKSSW

UniProtKB: Small ribosomal subunit protein uS14B

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Macromolecule #13: Small ribosomal subunit protein uS15

MacromoleculeName: Small ribosomal subunit protein uS15 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.079706 KDa
SequenceString:
ALTAEQKKEI LGQYGLHDTD TGSPEAQVAL LTKRIQDLTE HLKVHKHDHH SRRGLLLLVG RRRRLLKYVA QVDVARYRSL IERLGLR

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #14: Small ribosomal subunit protein bS16

MacromoleculeName: Small ribosomal subunit protein bS16 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 12.635652 KDa
SequenceString:
AVKIKLTRLG KIRNPQYRII VADARTRRDG RAIEVIGRYH PKEEPSLIQI DSERAQYWLG VGAQPTEPVL ALLKITGDWQ KFKGLPGAE GTLKVKEPKP SKLDLFNAAL AEAE

UniProtKB: Small ribosomal subunit protein bS16

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Macromolecule #15: Small ribosomal subunit protein uS17

MacromoleculeName: Small ribosomal subunit protein uS17 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 10.423152 KDa
SequenceString:
GPKYTPAAEK PRGRRKTAIG YVVSDKMQKT IVVELEDRKS HPLYGKIIRT TKKVKAHDEN GEAGIGDRVS LMETRPLSAT KRWRLVEIL EKA

UniProtKB: Small ribosomal subunit protein uS17

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Macromolecule #16: Small ribosomal subunit protein bS18B

MacromoleculeName: Small ribosomal subunit protein bS18B / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 7.338635 KDa
SequenceString:
KTRKCVFCSK KGQTIDYKDT ALLRTYISER GKIRARRVTG NCVQHQRDIA VAVKNAREVA LLPF

UniProtKB: Small ribosomal subunit protein bS18B

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Macromolecule #17: Small ribosomal subunit protein bS20

MacromoleculeName: Small ribosomal subunit protein bS20 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.35383 KDa
SequenceString:
NIKSQIKRIR TNERRRLRNQ SVKSSLRTAI RGFREAVDAG DKDKASELLH ATSRKLDKAA SKGVIHPNQA ANKKSALALA LNKL

UniProtKB: Small ribosomal subunit protein bS20

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Macromolecule #18: Small ribosomal subunit protein uS19

MacromoleculeName: Small ribosomal subunit protein uS19 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 9.167668 KDa
SequenceString:
KKGPFVDDHL LKKVDVQNEK NTKQVIKTWS RRSTIIPDFI GHTFAVHDGR KHVPVFVTEA MVGHKLGEFA PTRTFKGHIK

UniProtKB: Small ribosomal subunit protein uS19

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Macromolecule #19: Methionine aminopeptidase

MacromoleculeName: Methionine aminopeptidase / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO / EC number: methionyl aminopeptidase
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 32.354391 KDa
Recombinant expressionOrganism: Escherichia coli BL21(DE3) (bacteria)
SequenceString: MSVRSALRPG VLSPTLPVPK SIPRPEYAWK PTVQEGSEPW VQTPEVIEKM RVAGRIAAGA LAEAGRAVAP GVTTDELDRI AHEYMIDHG AYPSTLGYKG FPKSCCTSLN EIICHGIPDS TVIEDGDIVN IDVTAYIDGV HGDTNATFLA GDVSEEHRLL V ERTHEATM ...String:
MSVRSALRPG VLSPTLPVPK SIPRPEYAWK PTVQEGSEPW VQTPEVIEKM RVAGRIAAGA LAEAGRAVAP GVTTDELDRI AHEYMIDHG AYPSTLGYKG FPKSCCTSLN EIICHGIPDS TVIEDGDIVN IDVTAYIDGV HGDTNATFLA GDVSEEHRLL V ERTHEATM RAIKAVKPGR ALSVVGRVIE AYANRFGYNV VRDFTGHGIG TTFHNGLVVL HYDQPAVETV LEPGMTFTIE PM INLGTLD YEIWDDGWTV ATTDGKWTAQ FEHTLVVTED GAEILTQLAA ALQHHHHHH

UniProtKB: Methionine aminopeptidase

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Macromolecule #20: Small ribosomal subunit protein uS13

MacromoleculeName: Small ribosomal subunit protein uS13 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Mycolicibacterium smegmatis MC2 155 (bacteria)
Molecular weightTheoretical: 13.44556 KDa
SequenceString:
ARLVGVDLPR DKRMEIALTY IYGIGRTRSN EILAATGIDK NMRTKDLTDD QVTVLRDYIE GNLKVEGDLR REVQADIRRK IEIGCYQGL RHRRGLPVRG QRTKTNARTR KGPKRTIA

UniProtKB: Small ribosomal subunit protein uS13

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 20.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.3000000000000003 µm / Nominal defocus min: 1.8 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5zeu

Final reconstructionResolution.type: BY AUTHOR / Resolution: 4.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 50296
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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