Entry Database : EMDB / ID : EMD-39365 Downloads & linksTitle Cryo-EM structure of ADGRG2-Gs complex with NTF nanobody Map data Details SampleComplex : Complex of ADGRG2 with nanonody and G protein trimerProtein or peptide : Guanine nucleotide-binding protein G(s) subunit alphaProtein or peptide : Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1Protein or peptide : Guanine nucleotide-binding protein subunit gammaProtein or peptide : Nanobody-35Protein or peptide : Adhesion G-protein coupled receptor G2Protein or peptide : ScFv-16 Show more 2 items Show lessLigand : 3-BETA-HYDROXY-5-ANDROSTEN-17-ONE Details Keywords GPCR / MEMBRANE PROTEINFunction / homology Function and homology informationFunction Domain/homology Component
Olfactory Signaling Pathway / Sensory perception of sweet, bitter, and umami (glutamate) taste / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / sensory perception of chemical stimulus / Activation of the phototransduction cascade / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / beta-2 adrenergic receptor binding / Activation of G protein gated Potassium channels / G-protein activation ... Olfactory Signaling Pathway / Sensory perception of sweet, bitter, and umami (glutamate) taste / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / sensory perception of chemical stimulus / Activation of the phototransduction cascade / mu-type opioid receptor binding / corticotropin-releasing hormone receptor 1 binding / beta-2 adrenergic receptor binding / Activation of G protein gated Potassium channels / G-protein activation / G beta:gamma signalling through PI3Kgamma / Prostacyclin signalling through prostacyclin receptor / G beta:gamma signalling through PLC beta / ADP signalling through P2Y purinoceptor 1 / Thromboxane signalling through TP receptor / Presynaptic function of Kainate receptors / G beta:gamma signalling through CDC42 / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / G alpha (12/13) signalling events / Glucagon-type ligand receptors / G beta:gamma signalling through BTK / ADP signalling through P2Y purinoceptor 12 / Adrenaline,noradrenaline inhibits insulin secretion / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Ca2+ pathway / Thrombin signalling through proteinase activated receptors (PARs) / G alpha (z) signalling events / Extra-nuclear estrogen signaling / G alpha (s) signalling events / G alpha (q) signalling events / G alpha (i) signalling events / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells / Vasopressin regulates renal water homeostasis via Aquaporins / spermatid development / D1 dopamine receptor binding / adenylate cyclase-activating adrenergic receptor signaling pathway / insulin-like growth factor receptor binding / ionotropic glutamate receptor binding / adenylate cyclase activator activity / G protein-coupled receptor activity / G-protein beta/gamma-subunit complex binding / adenylate cyclase-activating G protein-coupled receptor signaling pathway / photoreceptor disc membrane / cellular response to catecholamine stimulus / adenylate cyclase-activating dopamine receptor signaling pathway / cellular response to prostaglandin E stimulus / G-protein beta-subunit binding / heterotrimeric G-protein complex / sensory perception of taste / signaling receptor complex adaptor activity / retina development in camera-type eye / GTPase binding / phospholipase C-activating G protein-coupled receptor signaling pathway / cell surface receptor signaling pathway / cell population proliferation / apical plasma membrane / G protein-coupled receptor signaling pathway / GTPase activity / synapse / GTP binding / protein-containing complex binding / metal ion binding / membrane / cytosol / cytoplasm Similarity search - Function GAIN domain superfamily / GPCR proteolysis site, GPS, motif / : / GPS motif / GAIN-B domain profile. / G-protein-coupled receptor proteolytic site domain / G-protein coupled receptors family 2 signature 2. / GPCR, family 2, secretin-like, conserved site / GPCR, family 2, secretin-like / 7 transmembrane receptor (Secretin family) ... GAIN domain superfamily / GPCR proteolysis site, GPS, motif / : / GPS motif / GAIN-B domain profile. / G-protein-coupled receptor proteolytic site domain / G-protein coupled receptors family 2 signature 2. / GPCR, family 2, secretin-like, conserved site / GPCR, family 2, secretin-like / 7 transmembrane receptor (Secretin family) / GPCR, family 2-like / G-protein coupled receptors family 2 profile 2. / G-protein alpha subunit, group S / Guanine nucleotide binding protein (G-protein), alpha subunit / G protein alpha subunit, helical insertion / G-protein alpha subunit / G-alpha domain profile. / G protein alpha subunit / G-protein, gamma subunit / G-protein gamma subunit domain profile. / G-protein gamma-like domain / G-protein gamma-like domain superfamily / GGL domain / G protein gamma subunit-like motifs / GGL domain / G protein beta WD-40 repeat protein / Guanine nucleotide-binding protein, beta subunit / G-protein, beta subunit / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology Guanine nucleotide-binding protein subunit gamma / Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 / Guanine nucleotide-binding protein G(s) subunit alpha / Adhesion G-protein coupled receptor G2 Similarity search - ComponentBiological species Bos taurus (domestic cattle) / Spodoptera (butterflies/moths)Method single particle reconstruction / cryo EM / Resolution : 2.9 Å Details AuthorsHuang SM / Sheng-chao G Funding support China, 1 items Details Hide detailsOrganization Grant number Country National Natural Science Foundation of China (NSFC) China
CitationJournal : Nat.Chem.Biol. / Year : 2025Title : Development of an allosteric adhesion GPCR nanobody with therapeutic potentialAuthors: Zheng Y / Jiang D / Lu Y / Zhang C / Huang SM / Lin H / Zhang D / Guo S / Han J / Chen J / He Y / Zhang M / Gao Y / Guo Y / Wei R / Xia M / Qin Y / Liu Z / Yang F / Ge S / Yi F / Yu X / Lin ... Authors : Zheng Y / Jiang D / Lu Y / Zhang C / Huang SM / Lin H / Zhang D / Guo S / Han J / Chen J / He Y / Zhang M / Gao Y / Guo Y / Wei R / Xia M / Qin Y / Liu Z / Yang F / Ge S / Yi F / Yu X / Lin H / Xiao P / Sun JP / Feng S History Deposition Mar 4, 2024 - Header (metadata) release Aug 13, 2025 - Map release Aug 13, 2025 - Update Aug 13, 2025 - Current status Aug 13, 2025 Processing site : PDBj / Status : Released
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