[English] 日本語

Yorodumi

- EMDB-39171: E.coli transcription translation coupling complex in TTC-P state ... -

+
Open data

-
Basic information

Entry

Database: EMDB / ID: EMD-39171

Title

E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin

Map data

Sample

Complex: Ternary complex of transcription-translation coupling complex
- Protein or peptide: x 57 types
- RNA: x 6 types
- DNA: x 2 types
Ligand: x 1 types

Keywords

ribosome / RNA polymerase / translation / transcription / coupling / transcription-translation coupling / expressome

Function / homology

Function and homology information

RNA polymerase complex / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / protein complex oligomerization / bacterial-type flagellum assembly / ornithine decarboxylase inhibitor activity ...RNA polymerase complex / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / protein complex oligomerization / bacterial-type flagellum assembly / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / nitrate assimilation / positive regulation of ribosome biogenesis / translational termination / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / ribosome assembly / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / response to reactive oxygen species / DNA endonuclease activity / transcription antitermination / DNA-templated transcription initiation / cell motility / translational initiation / regulation of cell growth / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / ribosome biogenesis / large ribosomal subunit / regulation of translation / ribosome binding / transferase activity / response to heat / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / protein-containing complex assembly / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / protein domain specific binding / response to antibiotic / nucleotide binding / negative regulation of DNA-templated transcription / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm
Similarity search - Function

Transcription termination factor NusA, C-terminal duplication / Transcription termination factor NusA / Transcription factor NusA, N-terminal / KH domain, NusA-like / NusA, N-terminal domain superfamily / NusA N-terminal domain / NusA-like KH domain / Transcription termination/antitermination protein NusA, bacterial / : / Transcription antitermination protein, NusG ...Transcription termination factor NusA, C-terminal duplication / Transcription termination factor NusA / Transcription factor NusA, N-terminal / KH domain, NusA-like / NusA, N-terminal domain superfamily / NusA N-terminal domain / NusA-like KH domain / Transcription termination/antitermination protein NusA, bacterial / : / Transcription antitermination protein, NusG / Transcription antitermination protein, NusG, bacteria, conserved site / Transcription termination factor nusG signature. / NusG-like / Transcription termination factor nusG / NusG, N-terminal / In Spt5p, this domain may confer affinity for Spt4p. It possesses a RNP-like fold. / DNA repair Rad51/transcription factor NusA, alpha-helical / Type-1 KH domain profile. / NusG, N-terminal domain superfamily / Helix-hairpin-helix domain / S1 domain profile. / DNA-directed RNA polymerase, omega subunit / DNA-directed RNA polymerase, subunit beta-prime, bacterial type / DNA-directed RNA polymerase, beta subunit, external 1 domain superfamily / DNA-directed RNA polymerase, beta subunit, external 1 domain / RNA polymerase beta subunit external 1 domain / RNA polymerase, alpha subunit, C-terminal / Bacterial RNA polymerase, alpha chain C terminal domain / DNA-directed RNA polymerase, alpha subunit / DNA-directed RNA polymerase beta subunit, bacterial-type / Ribosomal protein L11, bacterial-type / Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / Ribosomal protein L31 type A / Ribosomal protein S21 superfamily / S1 domain / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein L31 signature. / Ribosomal protein S21 / Ribosomal protein L31 / Ribosomal protein L31 superfamily / Ribosomal protein L31 / Ribosomal protein L11, conserved site / Ribosomal protein L11 signature. / Ribosomal protein L16 signature 1. / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 2. / Ribosomal protein L16, conserved site / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / : / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal protein L11, N-terminal / Ribosomal protein L11, N-terminal domain / Ribosomal protein L11/L12 / Ribosomal protein L11, C-terminal / Ribosomal protein L11, C-terminal domain superfamily / Ribosomal protein L11/L12, N-terminal domain superfamily / Ribosomal protein L11/L12 / Ribosomal protein L11, RNA binding domain / Ribosomal protein L17 signature. / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L36 signature. / Ribosomal protein L32p, bacterial type / Ribosomal protein L28/L24 superfamily / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / : / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L28 / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L18, bacterial-type / : / Ribosomal protein L6, bacterial-type / Ribosomal protein S19, bacterial-type / Ribosomal protein S3, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein L9/RNase H1, N-terminal
Similarity search - Domain/homology

Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL31 ...Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL31 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / DNA-directed RNA polymerase subunit alpha / DNA-directed RNA polymerase subunit omega / DNA-directed RNA polymerase subunit beta' / DNA-directed RNA polymerase subunit beta / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Transcription termination/antitermination protein NusA / Transcription termination/antitermination protein NusG / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25
Similarity search - Component

Biological species

Escherichia coli K-12 (bacteria) / synthetic construct (others)

Method

single particle reconstruction / cryo EM / Resolution: 4.55 Å

Authors

Zhang J / Lu G / Wang C / Lin J

Funding support

China, 2 items

Citation

Journal: To be published
Title: E.coli transcription translation coupling complex in TTC-P state 1 (subclass1) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin
Authors: Zhang J / Lu G / Ebright RH / Lin J / Wang C

History

Deposition	Feb 20, 2024	-
Header (metadata) release	Aug 27, 2025	-
Map release	Aug 27, 2025	-
Update	Aug 27, 2025	-
Current status	Aug 27, 2025	Processing site: PDBj / Status: Released

-
Structure visualization

-
Downloads & links

-
EMDB archive

Map data	emd_39171.map.gz	30.9 MB		EMDB map data format
Header (meta data)	emd-39171-v30.xml emd-39171.xml	85.1 KB 85.1 KB	Display Display	EMDB header
FSC (resolution estimation)	emd_39171_fsc.xml	16.9 KB	Display	FSC data file
Images	emd_39171.png	110.8 KB
Masks	emd_39171_msk_1.map	421.9 MB		Mask map
Filedesc metadata	emd-39171.cif.gz	17.3 KB
Others	emd_39171_half_map_1.map.gz emd_39171_half_map_2.map.gz	338.2 MB 339.2 MB
Archive directory	http://ftp.pdbj.org/pub/emdb/structures/EMD-39171 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-39171	HTTPS FTP

-
Validation report

Summary document	emd_39171_validation.pdf.gz	814.8 KB	Display	EMDB validaton report
Full document	emd_39171_full_validation.pdf.gz	814.3 KB	Display
Data in XML	emd_39171_validation.xml.gz	24.6 KB	Display
Data in CIF	emd_39171_validation.cif.gz	33.2 KB	Display
Arichive directory	https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-39171 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-39171	HTTPS FTP

-
Related structure data

Related structure data	8ydhMC M: atomic model generated by this map C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

-
Links

EMDB pages	EMDB (EBI/PDBe) / EMDataResource
Related items in Molecule of the Month	#253 - Jan 2021 Expressome similarity (115) #157 - Jan 2013 Transfer-Messenger RNA similarity (80) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (79) #48 - Dec 2003 Catabolite Activator Protein similarity (12) #121 - Jan 2010 70S Ribosomes similarity (17) #172 - Apr 2014 RecA and Rad51 similarity (2) #55 - Jul 2004 DNA Ligase similarity (1) #10 - Oct 2000 Ribosome similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (3) #266 - Feb 2022 Oligosaccharyltransferase similarity (2) #241 - Jan 2020 Twenty Years of Molecules similarity (4) #86 - Feb 2007 Exosomes similarity (1)

EMDB pages

EMDB (EBI/PDBe) /

EMDataResource

Related items in Molecule of the Month

#253 - Jan 2021
Expressome
similarity (115)
#157 - Jan 2013
Transfer-Messenger RNA
similarity (80)
#262 - Oct 2021
Fifty Years of Open Access to PDB Structures
similarity (79)
#48 - Dec 2003
Catabolite Activator Protein
similarity (12)
#121 - Jan 2010
70S Ribosomes
similarity (17)
#172 - Apr 2014
RecA and Rad51
similarity (2)
#55 - Jul 2004
DNA Ligase
similarity (1)
#10 - Oct 2000
Ribosome
similarity (1)
#255 - Mar 2021
Cisplatin and DNA
similarity (3)
#266 - Feb 2022
Oligosaccharyltransferase
similarity (2)
#241 - Jan 2020
Twenty Years of Molecules
similarity (4)
#86 - Feb 2007
Exosomes
similarity (1)

-
Map

File

Download / File: emd_39171.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)

Voxel size

X=Y=Z: 1.57 Å

Density

Contour Level	By AUTHOR: 0.011
Minimum - Maximum	-0.08518651 - 0.1879091
Average (Standard dev.)	0.00031070237 (±0.004995839)

Symmetry

Space group: 1

Details

-
Supplemental data

-
Sample components

+
Entire : Ternary complex of transcription-translation coupling complex

Entire	Name: Ternary complex of transcription-translation coupling complex
Components	Complex: Ternary complex of transcription-translation coupling complex Protein or peptide: 50S ribosomal protein L31 Protein or peptide: 50S ribosomal protein L32 Protein or peptide: 50S ribosomal protein L33 Protein or peptide: 50S ribosomal protein L34 Protein or peptide: 50S ribosomal protein L35 Protein or peptide: 50S ribosomal protein L36 Protein or peptide: 30S ribosomal protein S2 Protein or peptide: 30S ribosomal protein S3 Protein or peptide: 30S ribosomal protein S4 Protein or peptide: 30S ribosomal protein S5 Protein or peptide: 30S ribosomal protein S6, fully modified isoform Protein or peptide: 30S ribosomal protein S7 Protein or peptide: 30S ribosomal protein S8 Protein or peptide: 30S ribosomal protein S9 Protein or peptide: 30S ribosomal protein S10 Protein or peptide: 30S ribosomal protein S11 Protein or peptide: 30S ribosomal protein S12 Protein or peptide: 30S ribosomal protein S13 Protein or peptide: 30S ribosomal protein S14 Protein or peptide: 30S ribosomal protein S15 Protein or peptide: 30S ribosomal protein S16 Protein or peptide: 30S ribosomal protein S17 Protein or peptide: 30S ribosomal protein S18 Protein or peptide: 30S ribosomal protein S19 Protein or peptide: 30S ribosomal protein S20 Protein or peptide: 30S ribosomal protein S21 Protein or peptide: 50S ribosomal protein L2 Protein or peptide: 50S ribosomal protein L3 Protein or peptide: 50S ribosomal protein L4 Protein or peptide: 50S ribosomal protein L5 Protein or peptide: 50S ribosomal protein L6 Protein or peptide: 50S ribosomal protein L9 Protein or peptide: 50S ribosomal protein L11 Protein or peptide: 50S ribosomal protein L13 Protein or peptide: 50S ribosomal protein L14 Protein or peptide: 50S ribosomal protein L15 Protein or peptide: 50S ribosomal protein L16 Protein or peptide: 50S ribosomal protein L17 Protein or peptide: 50S ribosomal protein L18 Protein or peptide: 50S ribosomal protein L19 Protein or peptide: 50S ribosomal protein L20 Protein or peptide: 50S ribosomal protein L21 Protein or peptide: 50S ribosomal protein L22 Protein or peptide: 50S ribosomal protein L23 Protein or peptide: 50S ribosomal protein L24 Protein or peptide: 50S ribosomal protein L25 Protein or peptide: 50S ribosomal protein L27 Protein or peptide: 50S ribosomal protein L28 Protein or peptide: 50S ribosomal protein L29 Protein or peptide: 50S ribosomal protein L30 RNA: 23S rRNA RNA: 5S rRNA RNA: 16S rRNA RNA: mRNA DNA: templete DNA strand DNA: non-templete DNA strand Protein or peptide: DNA-directed RNA polymerase subunit alpha Protein or peptide: DNA-directed RNA polymerase subunit beta' Protein or peptide: DNA-directed RNA polymerase subunit beta Protein or peptide: DNA-directed RNA polymerase subunit omega Protein or peptide: Transcription termination/antitermination protein NusA Protein or peptide: Transcription termination/antitermination protein NusG RNA: tRNA(Phe) RNA: tRNA(fMet) Protein or peptide: tRNA(fMet) Ligand: MAGNESIUM ION

Entire

Name: Ternary complex of transcription-translation coupling complex

Components

Complex: Ternary complex of transcription-translation coupling complex
- Protein or peptide: 50S ribosomal protein L31
- Protein or peptide: 50S ribosomal protein L32
- Protein or peptide: 50S ribosomal protein L33
- Protein or peptide: 50S ribosomal protein L34
- Protein or peptide: 50S ribosomal protein L35
- Protein or peptide: 50S ribosomal protein L36
- Protein or peptide: 30S ribosomal protein S2
- Protein or peptide: 30S ribosomal protein S3
- Protein or peptide: 30S ribosomal protein S4
- Protein or peptide: 30S ribosomal protein S5
- Protein or peptide: 30S ribosomal protein S6, fully modified isoform
- Protein or peptide: 30S ribosomal protein S7
- Protein or peptide: 30S ribosomal protein S8
- Protein or peptide: 30S ribosomal protein S9
- Protein or peptide: 30S ribosomal protein S10
- Protein or peptide: 30S ribosomal protein S11
- Protein or peptide: 30S ribosomal protein S12
- Protein or peptide: 30S ribosomal protein S13
- Protein or peptide: 30S ribosomal protein S14
- Protein or peptide: 30S ribosomal protein S15
- Protein or peptide: 30S ribosomal protein S16
- Protein or peptide: 30S ribosomal protein S17
- Protein or peptide: 30S ribosomal protein S18
- Protein or peptide: 30S ribosomal protein S19
- Protein or peptide: 30S ribosomal protein S20
- Protein or peptide: 30S ribosomal protein S21
- Protein or peptide: 50S ribosomal protein L2
- Protein or peptide: 50S ribosomal protein L3
- Protein or peptide: 50S ribosomal protein L4
- Protein or peptide: 50S ribosomal protein L5
- Protein or peptide: 50S ribosomal protein L6
- Protein or peptide: 50S ribosomal protein L9
- Protein or peptide: 50S ribosomal protein L11
- Protein or peptide: 50S ribosomal protein L13
- Protein or peptide: 50S ribosomal protein L14
- Protein or peptide: 50S ribosomal protein L15
- Protein or peptide: 50S ribosomal protein L16
- Protein or peptide: 50S ribosomal protein L17
- Protein or peptide: 50S ribosomal protein L18
- Protein or peptide: 50S ribosomal protein L19
- Protein or peptide: 50S ribosomal protein L20
- Protein or peptide: 50S ribosomal protein L21
- Protein or peptide: 50S ribosomal protein L22
- Protein or peptide: 50S ribosomal protein L23
- Protein or peptide: 50S ribosomal protein L24
- Protein or peptide: 50S ribosomal protein L25
- Protein or peptide: 50S ribosomal protein L27
- Protein or peptide: 50S ribosomal protein L28
- Protein or peptide: 50S ribosomal protein L29
- Protein or peptide: 50S ribosomal protein L30
- RNA: 23S rRNA
- RNA: 5S rRNA
- RNA: 16S rRNA
- RNA: mRNA
- DNA: templete DNA strand
- DNA: non-templete DNA strand
- Protein or peptide: DNA-directed RNA polymerase subunit alpha
- Protein or peptide: DNA-directed RNA polymerase subunit beta'
- Protein or peptide: DNA-directed RNA polymerase subunit beta
- Protein or peptide: DNA-directed RNA polymerase subunit omega
- Protein or peptide: Transcription termination/antitermination protein NusA
- Protein or peptide: Transcription termination/antitermination protein NusG
- RNA: tRNA(Phe)
- RNA: tRNA(fMet)
- Protein or peptide: tRNA(fMet)
Ligand: MAGNESIUM ION

+
Supramolecule #1: Ternary complex of transcription-translation coupling complex

Supramolecule	Name: Ternary complex of transcription-translation coupling complex type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#65
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 3 MDa

+
Macromolecule #1: 50S ribosomal protein L31

Macromolecule	Name: 50S ribosomal protein L31 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 7.887117 KDa
Sequence	String: MKKDIHPKYE EITASCSCGN VMKIRSTVGH DLNLDVCSKC HPFFTGKQRD VATGGRVDRF NKRFNIPGSK UniProtKB: Large ribosomal subunit protein bL31

+
Macromolecule #2: 50S ribosomal protein L32

Macromolecule	Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 6.463445 KDa
Sequence	String: MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK UniProtKB: Large ribosomal subunit protein bL32

+
Macromolecule #3: 50S ribosomal protein L33

Macromolecule	Name: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 6.388631 KDa
Sequence	String: MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK UniProtKB: Large ribosomal subunit protein bL33

+
Macromolecule #4: 50S ribosomal protein L34

Macromolecule	Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 5.397463 KDa
Sequence	String: MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK UniProtKB: Large ribosomal subunit protein bL34

+
Macromolecule #5: 50S ribosomal protein L35

Macromolecule	Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 7.313032 KDa
Sequence	String: MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA UniProtKB: Large ribosomal subunit protein bL35

+
Macromolecule #6: 50S ribosomal protein L36

Macromolecule	Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 4.37739 KDa
Sequence	String: MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG UniProtKB: Large ribosomal subunit protein bL36A

+
Macromolecule #7: 30S ribosomal protein S2

Macromolecule	Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 26.78167 KDa
Sequence	String: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH ...String: MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E UniProtKB: Small ribosomal subunit protein uS2

+
Macromolecule #8: 30S ribosomal protein S3

Macromolecule	Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 26.031316 KDa
Sequence	String: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY ...String: MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK UniProtKB: Small ribosomal subunit protein uS3

+
Macromolecule #9: 30S ribosomal protein S4

Macromolecule	Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 23.514199 KDa
Sequence	String: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP ...String: MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK UniProtKB: Small ribosomal subunit protein uS4

+
Macromolecule #10: 30S ribosomal protein S5

Macromolecule	Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 17.629398 KDa
Sequence	String: MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK UniProtKB: Small ribosomal subunit protein uS5

+
Macromolecule #11: 30S ribosomal protein S6, fully modified isoform

Macromolecule	Name: 30S ribosomal protein S6, fully modified isoform / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 15.727512 KDa
Sequence	String: MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE UniProtKB: Small ribosomal subunit protein bS6

+
Macromolecule #12: 30S ribosomal protein S7

Macromolecule	Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 20.055156 KDa
Sequence	String: MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N UniProtKB: Small ribosomal subunit protein uS7

+
Macromolecule #13: 30S ribosomal protein S8

Macromolecule	Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 14.146557 KDa
Sequence	String: MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRK DELPKVMAGL GIAVVSTSKG VMTDRAARQA GLGGEIICYV A UniProtKB: Small ribosomal subunit protein uS8

+
Macromolecule #14: 30S ribosomal protein S9

Macromolecule	Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 14.88627 KDa
Sequence	String: MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R UniProtKB: Small ribosomal subunit protein uS9

+
Macromolecule #15: 30S ribosomal protein S10

Macromolecule	Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 11.755597 KDa
Sequence	String: MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG UniProtKB: Small ribosomal subunit protein uS10

+
Macromolecule #16: 30S ribosomal protein S11

Macromolecule	Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.870975 KDa
Sequence	String: MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP FAAQVAAERC ADAVKEYGIK NLEVMVKGP GPGRESTIRA LNAAGFRITN ITDVTPIPHN GCRPPKKRRV UniProtKB: Small ribosomal subunit protein uS11

+
Macromolecule #17: 30S ribosomal protein S12

Macromolecule	Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.768157 KDa
Sequence	String: MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVKD LPGVRYHTVR GALDCSGVKD RKQARSKYGV KRPKA UniProtKB: Small ribosomal subunit protein uS12

+
Macromolecule #18: 30S ribosomal protein S13

Macromolecule	Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.128467 KDa
Sequence	String: MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK UniProtKB: Small ribosomal subunit protein uS13

+
Macromolecule #19: 30S ribosomal protein S14

Macromolecule	Name: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 11.60656 KDa
Sequence	String: MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAM RGEIPGLKKA SW UniProtKB: Small ribosomal subunit protein uS14

+
Macromolecule #20: 30S ribosomal protein S15

Macromolecule	Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 10.290816 KDa
Sequence	String: MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV SQRRKLLDYL KRKDVARYTQ LIERLGLRR UniProtKB: Small ribosomal subunit protein uS15

+
Macromolecule #21: 30S ribosomal protein S16

Macromolecule	Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.207572 KDa
Sequence	String: MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA UniProtKB: Small ribosomal subunit protein bS16

+
Macromolecule #22: 30S ribosomal protein S17

Macromolecule	Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.724491 KDa
Sequence	String: MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL UniProtKB: Small ribosomal subunit protein uS17

+
Macromolecule #23: 30S ribosomal protein S18

Macromolecule	Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.005472 KDa
Sequence	String: MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ UniProtKB: Small ribosomal subunit protein bS18

+
Macromolecule #24: 30S ribosomal protein S19

Macromolecule	Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 10.455355 KDa
Sequence	String: MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV FVTDEMVGHK LGEFAPTRTY RGHAADKKA KKK UniProtKB: Small ribosomal subunit protein uS19

+
Macromolecule #25: 30S ribosomal protein S20

Macromolecule	Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.708464 KDa
Sequence	String: MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR QAAKGLIHKN KAARHKANLT AQINKLA UniProtKB: Small ribosomal subunit protein bS20

+
Macromolecule #26: 30S ribosomal protein S21

Macromolecule	Name: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 8.524039 KDa
Sequence	String: MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y UniProtKB: Small ribosomal subunit protein bS21

+
Macromolecule #27: 50S ribosomal protein L2

Macromolecule	Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 29.923619 KDa
Sequence	String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD ...String: MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK UniProtKB: Large ribosomal subunit protein uL2

+
Macromolecule #28: 50S ribosomal protein L3

Macromolecule	Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 22.277535 KDa
Sequence	String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE ...String: MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN QTPGKVFKGK K MAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A UniProtKB: Large ribosomal subunit protein uL3

+
Macromolecule #29: 50S ribosomal protein L4

Macromolecule	Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 22.121566 KDa
Sequence	String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD ...String: MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA UniProtKB: Large ribosomal subunit protein uL4

+
Macromolecule #30: 50S ribosomal protein L5

Macromolecule	Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 20.333611 KDa
Sequence	String: MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K UniProtKB: Large ribosomal subunit protein uL5

+
Macromolecule #31: 50S ribosomal protein L6

Macromolecule	Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 18.932791 KDa
Sequence	String: MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK UniProtKB: Large ribosomal subunit protein uL6

+
Macromolecule #32: 50S ribosomal protein L9

Macromolecule	Name: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 15.78902 KDa
Sequence	String: MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE UniProtKB: Large ribosomal subunit protein bL9

+
Macromolecule #33: 50S ribosomal protein L11

Macromolecule	Name: 50S ribosomal protein L11 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 14.894362 KDa
Sequence	String: MAKKVQAYVK LQVAAGMANP SPPVGPALGQ QGVNIMEFCK AFNAKTDSIE KGLPIPVVIT VYADRSFTFV TKTPPAAVLL KKAAGIKSG SGKPNKDKVG KISRAQLQEI AQTKAADMTG ADIEAMTRSI EGTARSMGLV VED UniProtKB: Large ribosomal subunit protein uL11

+
Macromolecule #34: 50S ribosomal protein L13

Macromolecule	Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 16.050606 KDa
Sequence	String: MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI UniProtKB: Large ribosomal subunit protein uL13

+
Macromolecule #35: 50S ribosomal protein L14

Macromolecule	Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.565067 KDa
Sequence	String: MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL UniProtKB: Large ribosomal subunit protein uL14

+
Macromolecule #36: 50S ribosomal protein L15

Macromolecule	Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 15.008471 KDa
Sequence	String: MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE UniProtKB: Large ribosomal subunit protein uL15

+
Macromolecule #37: 50S ribosomal protein L16

Macromolecule	Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 15.312269 KDa
Sequence	String: MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV RMGKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM UniProtKB: Large ribosomal subunit protein uL16

+
Macromolecule #38: 50S ribosomal protein L17

Macromolecule	Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 14.393657 KDa
Sequence	String: MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE UniProtKB: Large ribosomal subunit protein bL17

+
Macromolecule #39: 50S ribosomal protein L18

Macromolecule	Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 12.794668 KDa
Sequence	String: MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF UniProtKB: Large ribosomal subunit protein uL18

+
Macromolecule #40: 50S ribosomal protein L19

Macromolecule	Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.159278 KDa
Sequence	String: MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN UniProtKB: Large ribosomal subunit protein bL19

+
Macromolecule #41: 50S ribosomal protein L20

Macromolecule	Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 13.528024 KDa
Sequence	String: MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA UniProtKB: Large ribosomal subunit protein bL20

+
Macromolecule #42: 50S ribosomal protein L21

Macromolecule	Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 11.586374 KDa
Sequence	String: MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA UniProtKB: Large ribosomal subunit protein bL21

+
Macromolecule #43: 50S ribosomal protein L22

Macromolecule	Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 12.253359 KDa
Sequence	String: METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R UniProtKB: Large ribosomal subunit protein uL22

+
Macromolecule #44: 50S ribosomal protein L23

Macromolecule	Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 11.22216 KDa
Sequence	String: MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E UniProtKB: Large ribosomal subunit protein uL23

+
Macromolecule #45: 50S ribosomal protein L24

Macromolecule	Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 11.33925 KDa
Sequence	String: MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK UniProtKB: Large ribosomal subunit protein uL24

+
Macromolecule #46: 50S ribosomal protein L25

Macromolecule	Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 10.713465 KDa
Sequence	String: MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA UniProtKB: Large ribosomal subunit protein bL25

+
Macromolecule #47: 50S ribosomal protein L27

Macromolecule	Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.14654 KDa
Sequence	String: MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE UniProtKB: Large ribosomal subunit protein bL27

+
Macromolecule #48: 50S ribosomal protein L28

Macromolecule	Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 9.027551 KDa
Sequence	String: MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY UniProtKB: Large ribosomal subunit protein bL28

+
Macromolecule #49: 50S ribosomal protein L29

Macromolecule	Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 7.286464 KDa
Sequence	String: MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #50: 50S ribosomal protein L30

Macromolecule	Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 6.55482 KDa
Sequence	String: MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE UniProtKB: Large ribosomal subunit protein uL30

+
Macromolecule #57: DNA-directed RNA polymerase subunit alpha

Macromolecule	Name: DNA-directed RNA polymerase subunit alpha / type: protein_or_peptide / ID: 57 / Number of copies: 2 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 36.55868 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE IDGVLHEYST KEGVQEDILE ILLNLKGLA VRVQGKDEVI LTLNKSGIGP VTAADITHDG DVEIVKPQHV ICHLTDENAS ISMRIKVQRG RGYVPASTRI H SEEDERPI ...String: MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE IDGVLHEYST KEGVQEDILE ILLNLKGLA VRVQGKDEVI LTLNKSGIGP VTAADITHDG DVEIVKPQHV ICHLTDENAS ISMRIKVQRG RGYVPASTRI H SEEDERPI GRLLVDACYS PVERIAYNVE AARVEQRTDL DKLVIEMETN GTIDPEEAIR RAATILAEQL EAFVDLRDVR QP EVKEEKP EFDPILLRPV DDLELTVRSA NCLKAEAIHY IGDLVQRTEV ELLKTPNLGK KSLTEIKDVL ASRGLSLGMR LEN WPPASI ADE UniProtKB: DNA-directed RNA polymerase subunit alpha

+
Macromolecule #58: DNA-directed RNA polymerase subunit beta'

Macromolecule	Name: DNA-directed RNA polymerase subunit beta' / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 155.366781 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR IFGPVKDYEC LCGKYKRLKH RGVICEKCG VEVTQTKVRR ERMGHIELAS PTAHIWFLKS LPSRIGLLLD MPLRDIERVL YFESYVVIEG GMTNLERQQI L TEEQYLDA ...String: MKDLLKFLKA QTKTEEFDAI KIALASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR IFGPVKDYEC LCGKYKRLKH RGVICEKCG VEVTQTKVRR ERMGHIELAS PTAHIWFLKS LPSRIGLLLD MPLRDIERVL YFESYVVIEG GMTNLERQQI L TEEQYLDA LEEFGDEFDA KMGAEAIQAL LKSMDLEQEC EQLREELNET NSETKRKKLT KRIKLLEAFV QSGNKPEWMI LT VLPVLPP DLRPLVPLDG GRFATSDLND LYRRVINRNN RLKRLLDLAA PDIIVRNEKR MLQEAVDALL DNGRRGRAIT GSN KRPLKS LADMIKGKQG RFRQNLLGKR VDYSGRSVIT VGPYLRLHQC GLPKKMALEL FKPFIYGKLE LRGLATTIKA AKKM VEREE AVVWDILDEV IREHPVLLNR APTLHRLGIQ AFEPVLIEGK AIQLHPLVCA AYNADFDGDQ MAVHVPLTLE AQLEA RALM MSTNNILSPA NGEPIIVPSQ DVVLGLYYMT RDCVNAKGEG MVLTGPKEAE RLYRSGLASL HARVKVRITE YEKDAN GEL VAKTSLKDTT VGRAILWMIV PKGLPYSIVN QALGKKAISK MLNTCYRILG LKPTVIFADQ IMYTGFAYAA RSGASVG ID DMVIPEKKHE IISEAEAEVA EIQEQFQSGL VTAGERYNKV IDIWAAANDR VSKAMMDNLQ TETVINRDGQ EEKQVSFN S IYMMADSGAR GSAAQIRQLA GMRGLMAKPD GSIIETPITA NFREGLNVLQ YFISTHGARK GLADTALKTA NSGYLTRRL VDVAQDLVVT EDDCGTHEGI MMTPVIEGGD VKEPLRDRVL GRVTAEDVLK PGTADILVPR NTLLHEQWCD LLEENSVDAV KVRSVVSCD TDFGVCAHCY GRDLARGHII NKGEAIGVIA AQSIGEPGTQ LTMRTFHIGG AASRAAAESS IQVKNKGSIK L SNVKSVVN SSGKLVITSR NTELKLIDEF GRTKESYKVP YGAVLAKGDG EQVAGGETVA NWDPHTMPVI TEVSGFVRFT DM IDGQTIT RQTDELTGLS SLVVLDSAER TAGGKDLRPA LKIVDAQGND VLIPGTDMPA QYFLPGKAIV QLEDGVQISS GDT LARIPQ ESGGTKDITG GLPRVADLFE ARRPKEPAIL AEISGIVSFG KETKGKRRLV ITPVDGSDPY EEMIPKWRQL NVFE GERVE RGDVISDGPE APHDILRLRG VHAVTRYIVN EVQDVYRLQG VKINDKHIEV IVRQMLRKAT IVNAGSSDFL EGEQV EYSR VKIANRELEA NGKVGATYSR DLLGITKASL ATESFISAAS FQETTRVLTE AAVAGKRDEL RGLKENVIVG RLIPAG TGY AYHQDRMRRR AAGEAPAAPQ VTAEDASASL AELLNAGLGG SDNE UniProtKB: DNA-directed RNA polymerase subunit beta'

+
Macromolecule #59: DNA-directed RNA polymerase subunit beta

Macromolecule	Name: DNA-directed RNA polymerase subunit beta / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 150.820875 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE AAFRSVFPIQ SYSGNSELQY VSYRLGEPVF DVQECQIRG VTYSAPLRVK LRLVIYEREA PEGTVKDIKE QEVYMGEIPL MTDNGTFVIN GTERVIVSQL HRSPGVFFDS D KGKTHSSG ...String: MVYSYTEKKR IRKDFGKRPQ VLDVPYLLSI QLDSFQKFIE QDPEGQYGLE AAFRSVFPIQ SYSGNSELQY VSYRLGEPVF DVQECQIRG VTYSAPLRVK LRLVIYEREA PEGTVKDIKE QEVYMGEIPL MTDNGTFVIN GTERVIVSQL HRSPGVFFDS D KGKTHSSG KVLYNARIIP YRGSWLDFEF DPKDNLFVRI DRRRKLPATI ILRALNYTTE QILDLFFEKV IFEIRDNKLQ ME LVPERLR GETASFDIEA NGKVYVEKGR RITARHIRQL EKDDVKLIEV PVEYIAGKVV AKDYIDESTG ELICAANMEL SLD LLAKLS QSGHKRIETL FTNDLDHGPY ISETLRVDPT NDRLSALVEI YRMMRPGEPP TREAAESLFE NLFFSEDRYD LSAV GRMKF NRSLLREEIE GSGILSKDDI IDVMKKLIDI RNGKGEVDDI DHLGNRRIRS VGEMAENQFR VGLVRVERAV KERLS LGDL DTLMPQDMIN AKPISAAVKE FFGSSQLSQF MDQNNPLSEI THKRRISALG PGGLTRERAG FEVRDVHPTH YGRVCP IET PEGPNIGLIN SLSVYAQTNE YGFLETPYRK VTDGVVTDEI HYLSAIEEGN YVIAQANSNL DEEGHFVEDL VTCRSKG ES SLFSRDQVDY MDVSTQQVVS VGASLIPFLE HDDANRALMG ANMQRQAVPT LRADKPLVGT GMERAVAVDS GVTAVAKR G GVVQYVDASR IVIKVNEDEM YPGEAGIDIY NLTKYTRSNQ NTCINQMPCV SLGEPVERGD VLADGPSTDL GELALGQNM RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELACVS RDTKLGPEEI TADIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTP KGETQLTPEE KLLRAIFGEK ASDVKDSSLR VPNGVSGTVI DVQVFTRDGV EKDKRALEIE EMQLKQAKKD L SEELQILE AGLFSRIRAV LVAGGVEAEK LDKLPRDRWL ELGLTDEEKQ NQLEQLAEQY DELKHEFEKK LEAKRRKITQ GD DLAPGVL KIVKVYLAVK RRIQPGDKMA GRHGNKGVIS KINPIEDMPY DENGTPVDIV LNPLGVPSRM NIGQILETHL GMA AKGIGD KINAMLKQQQ EVAKLREFIQ RAYDLGADVR QKVDLSTFSD EEVMRLAENL RKGMPIATPV FDGAKEAEIK ELLK LGDLP TSGQIRLYDG RTGEQFERPV TVGYMYMLKL NHLVDDKMHA RSTGSYSLVT QQPLGGKAQF GGQRFGEMEV WALEA YGAA YTLQEMLTVK SDDVNGRTKM YKNIVDGNHQ MEPGMPESFN VLLKEIRSLG INIELEDE UniProtKB: DNA-directed RNA polymerase subunit beta

+
Macromolecule #60: DNA-directed RNA polymerase subunit omega

Macromolecule	Name: DNA-directed RNA polymerase subunit omega / type: protein_or_peptide / ID: 60 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 10.249547 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MARVTVQDAV EKIGNRFDLV LVAARRARQM QVGGKDPLVP EENDKTTVIA LREIEEGLIN NQILDVRERQ EQQEQEAAEL QAVTAIAEG RR UniProtKB: DNA-directed RNA polymerase subunit omega

+
Macromolecule #61: Transcription termination/antitermination protein NusA

Macromolecule	Name: Transcription termination/antitermination protein NusA type: protein_or_peptide / ID: 61 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 54.932684 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNL GDYVEDQIES VTFDRITTQT AKQVIVQKVR EAERAMVVDQ FREHEGEIIT GVVKKVNRDN ISLDLGNNAE A VILREDML ...String: MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNL GDYVEDQIES VTFDRITTQT AKQVIVQKVR EAERAMVVDQ FREHEGEIIT GVVKKVNRDN ISLDLGNNAE A VILREDML PRENFRPGDR VRGVLYSVRP EARGAQLFVT RSKPEMLIEL FRIEVPEIGE EVIEIKAAAR DPGSRAKIAV KT NDKRIDP VGACVGMRGA RVQAVSTELG GERIDIVLWD DNPAQFVINA MAPADVASIV VDEDKHTMDI AVEAGNLAQA IGR NGQNVR LASQLSGWEL NVMTVDDLQA KHQAEAHAAI DTFTKYLDID EDFATVLVEE GFSTLEELAY VPMKELLEIE GLDE PTVEA LRERAKNALA TIAQAQEESL GDNKPADDLL NLEGVDRDLA FKLAARGVCT LEDLAEQGID DLADIEGLTD EKAGA LIMA ARNICWFGDE A UniProtKB: Transcription termination/antitermination protein NusA

+
Macromolecule #62: Transcription termination/antitermination protein NusG

Macromolecule	Name: Transcription termination/antitermination protein NusG type: protein_or_peptide / ID: 62 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 20.560523 KDa
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV EIRGGQRRKS ERKFFPGYVL VQMVMNDASW HLVRSVPRV MGFIGGTSDR PAPISDKEVD AIMNRLQQVG DKPRPKTLFE PGEMVRVNDG PFADFNGVVE EVDYEKSRLK V SVSIFGRA TPVELDFSQV EKA UniProtKB: Transcription termination/antitermination protein NusG

+
Macromolecule #65: tRNA(fMet)

Macromolecule	Name: tRNA(fMet) / type: protein_or_peptide / ID: 65 / Number of copies: 1 / Enantiomer: DEXTRO
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 703.707 Da
Recombinant expression	Organism: Escherichia coli BL21(DE3) (bacteria)
Sequence	String: (KBE)(DPP)SS(UAL)(5OH)

+
Macromolecule #51: 23S rRNA

Macromolecule	Name: 23S rRNA / type: rna / ID: 51 / Number of copies: 1
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 941.611438 KDa
Sequence	String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG ...String: GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACGC UUAGGCGUGU GACUGC GUA CCUUUUGUAU AAUGGGUCAG CGACUUAUAU UCUGUAGCAA GGUUAACCGA AUAGGGGAGC CGAAGGGAAA CCGAGUC UU AACUGGGCGU UAAGUUGCAG GGUAUAGACC CGAAACCCGG UGAUCUAGCC AUGGGCAGGU UGAAGGUUGG GUAACACU A ACUGGAGGAC CGAACCGACU AAUGUCGAAA AAUUAGCGGA UGACUUGUGG CUGGGGGUGA AAGGCCAAUC AAACCGGGA GAUAGCUGGU UCUCCCCGAA AGCUAUUUAG GUAGCGCCUC GUGAAUUCAU CUCCGGGGGU AGAGCACUGU UUCGGCAAGG GGGUCAUCC CGACUUACCA ACCCGAUGCA AACUGCGAAU ACCGGAGAAU GUUAUCACGG GAGACACACG GCGGGUGCUA A CGUCCGUC GUGAAGAGGG AAACAACCCA GACCGCCAGC UAAGGUCCCA AAGUCAUGGU UAAGUGGGAA ACGAUGUGGG AA GGCCCAG ACAGCCAGGA UGUUGGCUUA GAAGCAGCCA UCAUUUAAAG AAAGCGUAAU AGCUCACUGG UCGAGUCGGC CUG CGCGGA AGAUGUAACG GGGCUAAACC AUGCACCGAA GCUGCGGCAG CGACGCUUAU GCGUUGUUGG GUAGGGGAGC GUUC UGUAA GCCUGCGAAG GUGUGCUGUG AGGCAUGCUG GAGGUAUCAG AAGUGCGAAU GCUGACAUAA GUAACGAUAA AGCGG GUGA AAAGCCCGCU CGCCGGAAGA CCAAGGGUUC CUGUCCAACG UUAAUCGGGG CAGGGUGAGU CGACCCCUAA GGCGAG GCC GAAAGGCGUA GUCGAUGGGA AACAGGUUAA UAUUCCUGUA CUUGGUGUUA CUGCGAAGGG GGGACGGAGA AGGCUAU GU UGGCCGGGCG ACGGUUGUCC CGGUUUAAGC GUGUAGGCUG GUUUUCCAGG CAAAUCCGGA AAAUCAAGGC UGAGGCGU G AUGACGAGGC ACUACGGUGC UGAAGCAACA AAUGCCCUGC UUCCAGGAAA AGCCUCUAAG CAUCAGGUAA CAUCAAAUC GUACCCCAAA CCGACACAGG UGGUCAGGUA GAGAAUACCA AGGCGCUUGA GAGAACUCGG GUGAAGGAAC UAGGCAAAAU GGUGCCGUA ACUUCGGGAG AAGGCACGCU GAUAUGUAGG UGAGGUCCCU CGCGGAUGGA GCUGAAAUCA GUCGAAGAUA C CAGCUGGC UGCAACUGUU UAUUAAAAAC ACAGCACUGU GCAAACACGA AAGUGGACGU AUACGGUGUG ACGCCUGCCC GG UGCCGGA AGGUUAAUUG AUGGGGUUAG CGCAAGCGAA GCUCUUGAUC GAAGCCCCGG UAAACGGCGG CCGUAACUAU AAC GGUCCU AAGGUAGCGA AAUUCCUUGU CGGGUAAGUU CCGACCUGCA CGAAUGGCGU AAUGAUGGCC AGGCUGUCUC CACC CGAGA CUCAGUGAAA UUGAACUCGC UGUGAAGAUG CAGUGUACCC GCGGCAAGAC GGAAAGACCC CGUGAACCUU UACUA UAGC UUGACACUGA ACAUUGAGCC UUGAUGUGUA GGAUAGGUGG GAGGCUUUGA AGUGUGGACG CCAGUCUGCA UGGAGC CGA CCUUGAAAUA CCACCCUUUA AUGUUUGAUG UUCUAACGUU GACCCGUAAU CCGGGUUGCG GACAGUGUCU GGUGGGU AG UUUGACUGGG GCGGUCUCCU CCUAAAGAGU AACGGAGGAG CACGAAGGUU GGCUAAUCCU GGUCGGACAU CAGGAGGU U AGUGCAAUGG CAUAAGCCAG CUUGACUGCG AGCGUGACGG CGCGAGCAGG UGCGAAAGCA GGUCAUAGUG AUCCGGUGG UUCUGAAUGG AAGGGCCAUC GCUCAACGGA UAAAAGGUAC UCCGGGGAUA ACAGGCUGAU ACCGCCCAAG AGUUCAUAUC GACGGCGGU GUUUGGCACC UCGAUGUCGG CUCAUCACAU CCUGGGGCUG AAGUAGGUCC CAAGGGUAUG GCUGUUCGCC A UUUAAAGU GGUACGCGAG CUGGGUUUAG AACGUCGUGA GACAGUUCGG UCCCUAUCUG CCGUGGGCGC UGGAGAACUG AG GGGGGCU GCUCCUAGUA CGAGAGGACC GGAGUGGACG CAUCACUGGU GUUCGGGUUG UCAUGCCAAU GGCACUGCCC GGU AGCUAA AUGCGGAAGA GAUAAGUGCU GAAAGCAUCU AAGCACGAAA CUUGCCCCGA GAUGAGUUCU CCCUGACCCU UUAA GGGUC CUGAAGGAAC GUUGAAGACG ACGACGUUGA UAGGCCGGGU GUGUAAGCGC AGCGAUGCGU UGAGCUAACC GGUAC UAAU GAACCGUGAG GCUUAACCUU GENBANK: GENBANK: CP063991.1

+
Macromolecule #52: 5S rRNA

Macromolecule	Name: 5S rRNA / type: rna / ID: 52 / Number of copies: 1
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 38.813133 KDa
Sequence	String: UGCCUGGCGG CCGUAGCGCG GUGGUCCCAC CUGACCCCAU GCCGAACUCA GAAGUGAAAC GCCGUAGCGC CGAUGGUAGU GUGGGGUCU CCCCAUGCGA GAGUAGGGAA CUGCCAGGCA A GENBANK: GENBANK: NR_103249.1

+
Macromolecule #53: 16S rRNA

Macromolecule	Name: 16S rRNA / type: rna / ID: 53 / Number of copies: 1
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 499.690031 KDa
Sequence	String: AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG ...String: AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGA AGCUUGCUU CUUUGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG CUAAUACCGC AUAACGUCGC AAGACCAAAG AGGGGGACCU UCGGGCCUCU UGCCAUCGGA UGUGCCCAGA UG GGAUUAG CUAGUAGGUG GGGUAACGGC UCACCUAGGC GACGAUCCCU AGCUGGUCUG AGAGGAUGAC CAGCCACACU GGA ACUGAG ACACGGUCCA GACUCCUACG GGAGGCAGCA GUGGGGAAUA UUGCACAAUG GGCGCAAGCC UGAUGCAGCC AUGC CGCGU GUAUGAAGAA GGCCUUCGGG UUGUAAAGUA CUUUCAGCGG GGAGGAAGGG AGUAAAGUUA AUACCUUUGC UCAUU GACG UUACCCGCAG AAGAAGCACC GGCUAACUCC GUGCCAGCAG CCGCGGUAAU ACGGAGGGUG CAAGCGUUAA UCGGAA UUA CUGGGCGUAA AGCGCACGCA GGCGGUUUGU UAAGUCAGAU GUGAAAUCCC CGGGCUCAAC CUGGGAACUG CAUCUGA UA CUGGCAAGCU UGAGUCUCGU AGAGGGGGGU AGAAUUCCAG GUGUAGCGGU GAAAUGCGUA GAGAUCUGGA GGAAUACC G GUGGCGAAGG CGGCCCCCUG GACGAAGACU GACGCUCAGG UGCGAAAGCG UGGGGAGCAA ACAGGAUUAG AUACCCUGG UAGUCCACGC CGUAAACGAU GUCGACUUGG AGGUUGUGCC CUUGAGGCGU GGCUUCCGGA GCUAACGCGU UAAGUCGACC GCCUGGGGA GUACGGCCGC AAGGUUAAAA CUCAAAUGAA UUGACGGGGG CCCGCACAAG CGGUGGAGCA UGUGGUUUAA U UCGAUGCA ACGCGAAGAA CCUUACCUGG UCUUGACAUC CACGGAAGUU UUCAGAGAUG AGAAUGUGCC UUCGGGAACC GU GAGACAG GUGCUGCAUG GCUGUCGUCA GCUCGUGUUG UGAAAUGUUG GGUUAAGUCC CGCAACGAGC GCAACCCUUA UCC UUUGUU GCCAGCGGUC CGGCCGGGAA CUCAAAGGAG ACUGCCAGUG AUAAACUGGA GGAAGGUGGG GAUGACGUCA AGUC AUCAU GGCCCUUACG ACCAGGGCUA CACACGUGCU ACAAUGGCGC AUACAAAGAG AAGCGACCUC GCGAGAGCAA GCGGA CCUC AUAAAGUGCG UCGUAGUCCG GAUUGGAGUC UGCAACUCGA CUCCAUGAAG UCGGAAUCGC UAGUAAUCGU GGAUCA GAA UGCCACGGUG AAUACGUUCC CGGGCCUUGU ACACACCGCC CGUCACACCA UGGGAGUGGG UUGCAAAAGA AGUAGGU AG CUUAACCUUC GGGAGGGCGC UUACCACUUU GUGAUUCAUG ACUGGGGUGA AGUCGUAACA AGGUAACCGU AGGGGAAC C UGCGGUUGGA UCACCUCCUU A GENBANK: GENBANK: CP047127.1

+
Macromolecule #54: mRNA

Macromolecule	Name: mRNA / type: rna / ID: 54 / Number of copies: 1
Source (natural)	Organism: synthetic construct (others)
Molecular weight	Theoretical: 14.747342 KDa
Sequence	String: AUGUUUCUUC UUUUUUUUUU UUUUUUUUUU UUUGAUUUGG UGAGAGG

+
Macromolecule #63: tRNA(Phe)

Macromolecule	Name: tRNA(Phe) / type: rna / ID: 63 / Number of copies: 1
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 24.485539 KDa
Sequence	String: GCCCGGAUAG CUCAGUCGGU AGAGCAGGGG AUUGAAAAUC CCCGUGUCCU UGGUUCGAUU CCGAGUCCGG GCACCA GENBANK: GENBANK: CP053605.1

+
Macromolecule #64: tRNA(fMet)

Macromolecule	Name: tRNA(fMet) / type: rna / ID: 64 / Number of copies: 2
Source (natural)	Organism: Escherichia coli K-12 (bacteria)
Molecular weight	Theoretical: 24.802785 KDa
Sequence	String: CGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUCAUAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA GENBANK: GENBANK: CP053605.1

+
Macromolecule #55: templete DNA strand

Macromolecule	Name: templete DNA strand / type: dna / ID: 55 / Number of copies: 1 / Classification: DNA
Source (natural)	Organism: synthetic construct (others)
Molecular weight	Theoretical: 11.230182 KDa
Sequence	String: (DC)(DC)(DC)(DT)(DG)(DT)(DC)(DT)(DG)(DG) (DC)(DG)(DT)(DC)(DC)(DT)(DC)(DT)(DC)(DA) (DC)(DC)(DC)(DC)(DA)(DT)(DG)(DA)(DT) (DC)(DA)(DT)(DG)(DA)(DC)(DG)(DG)

+
Macromolecule #56: non-templete DNA strand

Macromolecule	Name: non-templete DNA strand / type: dna / ID: 56 / Number of copies: 1 / Classification: DNA
Source (natural)	Organism: synthetic construct (others)
Molecular weight	Theoretical: 11.473346 KDa
Sequence	String: (DC)(DC)(DG)(DT)(DC)(DA)(DT)(DG)(DA)(DT) (DC)(DA)(DT)(DG)(DG)(DC)(DG)(DG)(DT)(DA) (DG)(DC)(DG)(DT)(DG)(DC)(DG)(DC)(DC) (DA)(DG)(DA)(DC)(DA)(DG)(DG)(DG)

+
Macromolecule #66: MAGNESIUM ION

-
Experimental details

-
Structure determination

Method	cryo EM
Processing	single particle reconstruction
Aggregation state	particle

-
Sample preparation

Buffer	pH: 7.4
Vitrification	Cryogen name: ETHANE

-
Electron microscopy

Microscope	FEI TALOS ARCTICA
Image recording	Film or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 47.0 e/Å²
Electron beam	Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
Electron optics	Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm
Experimental equipment	Model: Talos Arctica / Image courtesy: FEI Company

+
Image processing

CTF correction	Type: NONE
Startup model	Type of model: NONE
Final reconstruction	Resolution.type: BY AUTHOR / Resolution: 4.55 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 23023
Initial angle assignment	Type: ANGULAR RECONSTITUTION
Final angle assignment	Type: ANGULAR RECONSTITUTION

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

Version 3 of the EMDB header file is now the official format.
The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

+
Jul 12, 2017. Major update of PDB

-
Yorodumi

Thousand views of thousand structures

Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Macromolecule	Name: MAGNESIUM ION / type: ligand / ID: 66 / Number of copies: 1 / Formula: MG
Molecular weight	Theoretical: 24.305 Da

-Movie

-Structure viewers

+Viewer log

-This page

-This web site

-Options

+Open data

-Basic information

-Structure visualization

-Downloads & links

-EMDB archive

-Validation report

-Related structure data

-Links

-Map

-Supplemental data

-Sample components

+Entire : Ternary complex of transcription-translation coupling complex

+Supramolecule #1: Ternary complex of transcription-translation coupling complex

+Macromolecule #1: 50S ribosomal protein L31

+Macromolecule #2: 50S ribosomal protein L32

+Macromolecule #3: 50S ribosomal protein L33

+Macromolecule #4: 50S ribosomal protein L34

+Macromolecule #5: 50S ribosomal protein L35

+Macromolecule #6: 50S ribosomal protein L36

+Macromolecule #7: 30S ribosomal protein S2

+Macromolecule #8: 30S ribosomal protein S3

+Macromolecule #9: 30S ribosomal protein S4

+Macromolecule #10: 30S ribosomal protein S5

+Macromolecule #11: 30S ribosomal protein S6, fully modified isoform

+Macromolecule #12: 30S ribosomal protein S7

+Macromolecule #13: 30S ribosomal protein S8

+Macromolecule #14: 30S ribosomal protein S9

+Macromolecule #15: 30S ribosomal protein S10

+Macromolecule #16: 30S ribosomal protein S11

+Macromolecule #17: 30S ribosomal protein S12

+Macromolecule #18: 30S ribosomal protein S13

+Macromolecule #19: 30S ribosomal protein S14

+Macromolecule #20: 30S ribosomal protein S15

+Macromolecule #21: 30S ribosomal protein S16

+Macromolecule #22: 30S ribosomal protein S17

+Macromolecule #23: 30S ribosomal protein S18

+Macromolecule #24: 30S ribosomal protein S19

+Macromolecule #25: 30S ribosomal protein S20

+Macromolecule #26: 30S ribosomal protein S21

+Macromolecule #27: 50S ribosomal protein L2

+Macromolecule #28: 50S ribosomal protein L3

+Macromolecule #29: 50S ribosomal protein L4

+Macromolecule #30: 50S ribosomal protein L5

+Macromolecule #31: 50S ribosomal protein L6

+Macromolecule #32: 50S ribosomal protein L9

+Macromolecule #33: 50S ribosomal protein L11

+Macromolecule #34: 50S ribosomal protein L13

+Macromolecule #35: 50S ribosomal protein L14

+Macromolecule #36: 50S ribosomal protein L15

+Macromolecule #37: 50S ribosomal protein L16

+Macromolecule #38: 50S ribosomal protein L17

+Macromolecule #39: 50S ribosomal protein L18

+Macromolecule #40: 50S ribosomal protein L19

+Macromolecule #41: 50S ribosomal protein L20

+Macromolecule #42: 50S ribosomal protein L21

+Macromolecule #43: 50S ribosomal protein L22

+Macromolecule #44: 50S ribosomal protein L23

+Macromolecule #45: 50S ribosomal protein L24

+Macromolecule #46: 50S ribosomal protein L25

+Macromolecule #47: 50S ribosomal protein L27

+Macromolecule #48: 50S ribosomal protein L28

+Macromolecule #49: 50S ribosomal protein L29

+Macromolecule #50: 50S ribosomal protein L30

+Macromolecule #57: DNA-directed RNA polymerase subunit alpha

+Macromolecule #58: DNA-directed RNA polymerase subunit beta'

+Macromolecule #59: DNA-directed RNA polymerase subunit beta

+Macromolecule #60: DNA-directed RNA polymerase subunit omega

+Macromolecule #61: Transcription termination/antitermination protein NusA

+Macromolecule #62: Transcription termination/antitermination protein NusG

+Macromolecule #65: tRNA(fMet)

+Macromolecule #51: 23S rRNA

+Macromolecule #52: 5S rRNA

+Macromolecule #53: 16S rRNA

+Macromolecule #54: mRNA

-
Movie

-
Structure viewers

+
Viewer log

-
This page

-
This web site

-
Options