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データを開く
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基本情報
登録情報 | ![]() | |||||||||
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タイトル | E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin | |||||||||
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![]() | ribosome / RNA polymerase / translation / transcription / coupling / transcription-translation coupling / expressome | |||||||||
機能・相同性 | ![]() RNA polymerase complex / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / protein complex oligomerization / bacterial-type flagellum assembly / ornithine decarboxylase inhibitor activity ...RNA polymerase complex / negative regulation of cytoplasmic translational initiation / transcription elongation-coupled chromatin remodeling / submerged biofilm formation / stringent response / cellular response to cell envelope stress / regulation of DNA-templated transcription initiation / protein complex oligomerization / bacterial-type flagellum assembly / ornithine decarboxylase inhibitor activity / transcription antitermination factor activity, RNA binding / bacterial-type RNA polymerase core enzyme binding / cytosolic DNA-directed RNA polymerase complex / misfolded RNA binding / Group I intron splicing / RNA folding / bacterial-type flagellum-dependent cell motility / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / nitrate assimilation / positive regulation of ribosome biogenesis / translational termination / negative regulation of cytoplasmic translation / four-way junction DNA binding / DnaA-L2 complex / translation repressor activity / negative regulation of translational initiation / regulation of mRNA stability / negative regulation of DNA-templated DNA replication initiation / mRNA regulatory element binding translation repressor activity / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / ribosome assembly / transcription elongation factor complex / regulation of DNA-templated transcription elongation / cytosolic ribosome assembly / response to reactive oxygen species / DNA endonuclease activity / transcription antitermination / DNA-templated transcription initiation / cell motility / translational initiation / regulation of cell growth / DNA-templated transcription termination / response to radiation / maintenance of translational fidelity / mRNA 5'-UTR binding / ribonucleoside binding / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / ribosome biogenesis / large ribosomal subunit / regulation of translation / ribosome binding / transferase activity / response to heat / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / protein-containing complex assembly / small ribosomal subunit rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / intracellular iron ion homeostasis / cytoplasmic translation / tRNA binding / protein dimerization activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / ribosome / translation / DNA-binding transcription factor activity / protein domain specific binding / response to antibiotic / nucleotide binding / negative regulation of DNA-templated transcription / mRNA binding / magnesium ion binding / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() | |||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 5.0 Å | |||||||||
![]() | Zhang J / Lu G / Wang C / Lin J | |||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: E.coli transcription translation coupling complex in TTC-B state 1 (subclass 3) containing mRNA with 39-mer spacer, NusG, NusA, fMet-tRNA(iMet), Phe-tRNA(Phe), and viomycin 著者: Zhang J / Lu G / Ebright RH / Lin J / Wang C | |||||||||
履歴 |
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構造の表示
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ダウンロードとリンク
-EMDBアーカイブ
マップデータ | ![]() | 30.3 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 84.9 KB 84.9 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 17 KB | 表示 | ![]() |
画像 | ![]() | 107.5 KB | ||
マスクデータ | ![]() | 421.9 MB | ![]() | |
Filedesc metadata | ![]() | 17.3 KB | ||
その他 | ![]() ![]() | 338.9 MB 338.6 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 852.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 852.1 KB | 表示 | |
XML形式データ | ![]() | 24.6 KB | 表示 | |
CIF形式データ | ![]() | 33.2 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 8ydeMC M: このマップから作成された原子モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 1.57 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
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試料の構成要素
+全体 : Ternary complex of transcription-translation coupling complex
+超分子 #1: Ternary complex of transcription-translation coupling complex
+分子 #1: 50S ribosomal protein L31
+分子 #2: 50S ribosomal protein L32
+分子 #3: 50S ribosomal protein L33
+分子 #4: 50S ribosomal protein L34
+分子 #5: 50S ribosomal protein L35
+分子 #6: 50S ribosomal protein L36
+分子 #7: 30S ribosomal protein S2
+分子 #8: 30S ribosomal protein S3
+分子 #9: 30S ribosomal protein S4
+分子 #10: 30S ribosomal protein S5
+分子 #11: 30S ribosomal protein S6, fully modified isoform
+分子 #12: 30S ribosomal protein S7
+分子 #13: 30S ribosomal protein S8
+分子 #14: 30S ribosomal protein S9
+分子 #15: 30S ribosomal protein S10
+分子 #16: 30S ribosomal protein S11
+分子 #17: 30S ribosomal protein S12
+分子 #18: 30S ribosomal protein S13
+分子 #19: 30S ribosomal protein S14
+分子 #20: 30S ribosomal protein S15
+分子 #21: 30S ribosomal protein S16
+分子 #22: 30S ribosomal protein S17
+分子 #23: 30S ribosomal protein S18
+分子 #24: 30S ribosomal protein S19
+分子 #25: 30S ribosomal protein S20
+分子 #26: 30S ribosomal protein S21
+分子 #27: 50S ribosomal protein L2
+分子 #28: 50S ribosomal protein L3
+分子 #29: 50S ribosomal protein L4
+分子 #30: 50S ribosomal protein L5
+分子 #31: 50S ribosomal protein L6
+分子 #32: 50S ribosomal protein L9
+分子 #33: 50S ribosomal protein L11
+分子 #34: 50S ribosomal protein L13
+分子 #35: 50S ribosomal protein L14
+分子 #36: 50S ribosomal protein L15
+分子 #37: 50S ribosomal protein L16
+分子 #38: 50S ribosomal protein L17
+分子 #39: 50S ribosomal protein L18
+分子 #40: 50S ribosomal protein L19
+分子 #41: 50S ribosomal protein L20
+分子 #42: 50S ribosomal protein L21
+分子 #43: 50S ribosomal protein L22
+分子 #44: 50S ribosomal protein L23
+分子 #45: 50S ribosomal protein L24
+分子 #46: 50S ribosomal protein L25
+分子 #47: 50S ribosomal protein L27
+分子 #48: 50S ribosomal protein L28
+分子 #49: 50S ribosomal protein L29
+分子 #50: 50S ribosomal protein L30
+分子 #57: DNA-directed RNA polymerase subunit alpha
+分子 #58: DNA-directed RNA polymerase subunit beta'
+分子 #59: DNA-directed RNA polymerase subunit beta
+分子 #60: DNA-directed RNA polymerase subunit omega
+分子 #61: Transcription termination/antitermination protein NusA
+分子 #62: Transcription termination/antitermination protein NusG
+分子 #65: Viomycin
+分子 #51: 23S rRNA
+分子 #52: 5S rRNA
+分子 #53: 16S rRNA
+分子 #54: mRNA
+分子 #63: tRNA(Phe)
+分子 #64: tRNA(fMet)
+分子 #55: templete DNA strand
+分子 #56: non-templete DNA strand
+分子 #66: MAGNESIUM ION
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.4 |
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凍結 | 凍結剤: ETHANE |
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電子顕微鏡法
顕微鏡 | FEI TALOS ARCTICA |
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撮影 | フィルム・検出器のモデル: FEI FALCON III (4k x 4k) 平均電子線量: 47.0 e/Å2 |
電子線 | 加速電圧: 200 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 1.5 µm / 最小 デフォーカス(公称値): 0.5 µm |
実験機器 | ![]() モデル: Talos Arctica / 画像提供: FEI Company |