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- EMDB-38950: 45S state a under 1 mM magnesium when overexpressing wild type DbpA -
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Open data
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Basic information
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Title | 45S state a under 1 mM magnesium when overexpressing wild type DbpA | |||||||||
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![]() | pre50S / ribosome / DbpA | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.5 Å | |||||||||
![]() | Yu T / Zeng F | |||||||||
Funding support | ![]()
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![]() | ![]() Title: PTC remodeling in pre50S intermediates: insights into the role of DEAD-box RNA helicase DbpA Authors: Yu T / Yu Q / Jiang J / Li X / Huang Z / Zeng F | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 18 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 11.5 KB 11.5 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.6 KB | Display | ![]() |
Images | ![]() | 67 KB | ||
Filedesc metadata | ![]() | 3.7 KB | ||
Others | ![]() ![]() | 171.4 MB 171.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 931.4 KB | Display | ![]() |
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Full document | ![]() | 931 KB | Display | |
Data in XML | ![]() | 21 KB | Display | |
Data in CIF | ![]() | 27.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.05 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_38950_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_38950_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : 45S state a under 1 mM magnesium when overexpressing wild type DbpA
Entire | Name: 45S state a under 1 mM magnesium when overexpressing wild type DbpA |
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Components |
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-Supramolecule #1: 45S state a under 1 mM magnesium when overexpressing wild type DbpA
Supramolecule | Name: 45S state a under 1 mM magnesium when overexpressing wild type DbpA type: complex / ID: 1 / Parent: 0 |
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Source (natural) | Organism: ![]() ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |