+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-38596 | |||||||||
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Title | Cryo-EM structure of cryptophyte photosystem II | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Cryptophyte / Photosystem II / evolution / PHOTOSYNTHESIS | |||||||||
Function / homology | Function and homology information thylakoid membrane / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / photosynthetic electron transport in photosystem II ...thylakoid membrane / photosystem II stabilization / photosystem II reaction center / photosystem II / oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor / photosynthetic electron transport chain / response to herbicide / photosystem II / plastid / photosynthetic electron transport in photosystem II / chlorophyll binding / photosynthesis, light reaction / chloroplast thylakoid membrane / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / phosphate ion binding / photosynthesis / respiratory electron transport chain / electron transfer activity / oxidoreductase activity / protein stabilization / iron ion binding / heme binding Similarity search - Function | |||||||||
Biological species | Chroomonas placoidea (eukaryote) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.53 Å | |||||||||
Authors | Li K / Zhao LS / Zhang YZ / Liu LN | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Cryo-EM structure of cryptophyte photosystem II Authors: Li K / Zhao LS / Zhang YZ / Liu LN | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_38596.map.gz | 56.2 MB | EMDB map data format | |
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Header (meta data) | emd-38596-v30.xml emd-38596.xml | 45.3 KB 45.3 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_38596_fsc.xml | 13.1 KB | Display | FSC data file |
Images | emd_38596.png | 203.7 KB | ||
Filedesc metadata | emd-38596.cif.gz | 10.1 KB | ||
Others | emd_38596_half_map_1.map.gz emd_38596_half_map_2.map.gz | 59.6 MB 59.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-38596 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-38596 | HTTPS FTP |
-Validation report
Summary document | emd_38596_validation.pdf.gz | 980.6 KB | Display | EMDB validaton report |
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Full document | emd_38596_full_validation.pdf.gz | 980.2 KB | Display | |
Data in XML | emd_38596_validation.xml.gz | 18.2 KB | Display | |
Data in CIF | emd_38596_validation.cif.gz | 24.4 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-38596 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-38596 | HTTPS FTP |
-Related structure data
Related structure data | 8xr6MC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_38596.map.gz / Format: CCP4 / Size: 64 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Structure of cryptophyte photosystem II
File | emd_38596_half_map_1.map | ||||||||||||
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Annotation | Structure of cryptophyte photosystem II | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Structure of cryptophyte photosystem II
File | emd_38596_half_map_2.map | ||||||||||||
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Annotation | Structure of cryptophyte photosystem II | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
+Entire : Photosystem II of cryptophyte
+Supramolecule #1: Photosystem II of cryptophyte
+Macromolecule #1: Photosystem II protein D1
+Macromolecule #2: ACPII-4
+Macromolecule #3: ACPII-1
+Macromolecule #4: ACPII-2
+Macromolecule #5: ACPII-3
+Macromolecule #6: ACPII-5
+Macromolecule #7: ACPII-6
+Macromolecule #8: Photosystem II CP47 reaction center protein
+Macromolecule #9: PsbC_CP43
+Macromolecule #10: Photosystem II D2 protein
+Macromolecule #11: Cytochrome b559 subunit alpha
+Macromolecule #12: Cytochrome b559 subunit beta
+Macromolecule #13: Photosystem II reaction center protein H
+Macromolecule #14: Photosystem II reaction center protein I
+Macromolecule #15: Photosystem II reaction center protein J
+Macromolecule #16: Photosystem II reaction center protein K
+Macromolecule #17: Photosystem II reaction center protein L
+Macromolecule #18: PsbM
+Macromolecule #19: Psb-gama_linker
+Macromolecule #20: PsbO
+Macromolecule #21: PsbQ
+Macromolecule #22: Photosystem II reaction center protein T
+Macromolecule #23: PsbU
+Macromolecule #24: Photosystem II cytochrome c550
+Macromolecule #25: PsbW
+Macromolecule #26: Photosystem II reaction center protein X
+Macromolecule #27: Photosystem II reaction center protein Psb30
+Macromolecule #28: Photosystem II reaction center protein Z
+Macromolecule #29: CA-MN4-O5 CLUSTER
+Macromolecule #30: FE (II) ION
+Macromolecule #31: BICARBONATE ION
+Macromolecule #32: CHLORIDE ION
+Macromolecule #33: CHLOROPHYLL A
+Macromolecule #34: PHEOPHYTIN A
+Macromolecule #35: (6'R,11cis,11'cis,13cis,15cis)-4',5'-didehydro-5',6'-dihydro-beta...
+Macromolecule #36: 2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,...
+Macromolecule #37: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
+Macromolecule #38: Chlorophyll c2
+Macromolecule #39: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #40: (1~{R})-3,5,5-trimethyl-4-[(3~{E},5~{E},7~{E},9~{E},11~{E},13~{E}...
+Macromolecule #41: 1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL
+Macromolecule #42: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
+Macromolecule #43: DIGALACTOSYL DIACYL GLYCEROL (DGDG)
+Macromolecule #44: PROTOPORPHYRIN IX CONTAINING FE
+Macromolecule #45: water
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 6.5 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |