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- EMDB-38523: VMAT2 complex with noradrenaline in cytosol-facing state -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-38523
TitleVMAT2 complex with noradrenaline in cytosol-facing state
Map data
Sample
  • Complex: complex with NE
    • Protein or peptide: Synaptic vesicular amine transporter,Synaptic vesicular amine transporter,transporter A
  • Ligand: Noradrenaline
KeywordstransporterA / TRANSPORT PROTEIN
Function / homology
Function and homology information


serotonin secretion by mast cell / somato-dendritic dopamine secretion / histamine uptake / aminergic neurotransmitter loading into synaptic vesicle / neurotransmitter loading into synaptic vesicle / monoamine:proton antiporter activity / clathrin-sculpted monoamine transport vesicle membrane / Serotonin Neurotransmitter Release Cycle / serotonin:sodium:chloride symporter activity / Dopamine Neurotransmitter Release Cycle ...serotonin secretion by mast cell / somato-dendritic dopamine secretion / histamine uptake / aminergic neurotransmitter loading into synaptic vesicle / neurotransmitter loading into synaptic vesicle / monoamine:proton antiporter activity / clathrin-sculpted monoamine transport vesicle membrane / Serotonin Neurotransmitter Release Cycle / serotonin:sodium:chloride symporter activity / Dopamine Neurotransmitter Release Cycle / Norepinephrine Neurotransmitter Release Cycle / serotonin uptake / dopamine transport / monoamine transmembrane transporter activity / histamine secretion by mast cell / monoamine transport / dopaminergic synapse / SLC-mediated transport of neurotransmitters / neurotransmitter transport / negative regulation of reactive oxygen species biosynthetic process / secretory granule membrane / response to amphetamine / post-embryonic development / locomotory behavior / response to toxic substance / terminal bouton / synaptic vesicle / synaptic vesicle membrane / chemical synaptic transmission / axon / intracellular membrane-bounded organelle / dendrite / centrosome / membrane / plasma membrane
Similarity search - Function
: / Major facilitator superfamily / Major Facilitator Superfamily / Major facilitator superfamily domain / Major facilitator superfamily (MFS) profile. / MFS transporter superfamily
Similarity search - Domain/homology
Synaptic vesicular amine transporter
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.2 Å
AuthorsJiang DH / Wu D
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31971134 China
CitationJournal: Cell Res / Year: 2024
Title: Structural snapshots of human VMAT2 reveal insights into substrate recognition and proton coupling mechanism.
Authors: Di Wu / Zhuoya Yu / Qihao Chen / Jun Zhao / Bo Huang / Yuhang Wang / Jiawei Su / Na Li / Daohua Jiang / Yan Zhao /
History
DepositionDec 31, 2023-
Header (metadata) releaseMay 8, 2024-
Map releaseMay 8, 2024-
UpdateJul 2, 2025-
Current statusJul 2, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_38523.png

Downloads & links

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Map

FileDownload / File: emd_38523.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 320 pix.
= 272. Å		0.85 Å/pix.
x 320 pix.
= 272. Å		0.85 Å/pix.
x 320 pix.
= 272. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.85 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.58
Minimum - Maximum-3.600985 - 5.273455
Average (Standard dev.)0.001390555 (±0.057099245)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions320320320
Spacing320320320
CellA=B=C: 272.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_38523_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_38523_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : complex with NE

EntireName: complex with NE
Components
  • Complex: complex with NE
    • Protein or peptide: Synaptic vesicular amine transporter,Synaptic vesicular amine transporter,transporter A
  • Ligand: Noradrenaline

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Supramolecule #1: complex with NE

SupramoleculeName: complex with NE / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: Synaptic vesicular amine transporter,Synaptic vesicular amine tra...

MacromoleculeName: Synaptic vesicular amine transporter,Synaptic vesicular amine transporter,transporter A
type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 61.953148 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: RSRKLILFIV FLALLLDNML LTVVVPIIPS YLYSIKHEKN ATEIQTARPV HTASISDSFQ SIFSYYDNST MVTGNATRDL TLHQTATQH MVTNASAVPS DCPSEDKDLL NENVQVGLLF ASKATVQLIT NPFIGLLTNR IGYPIPIFAG FCIMFVSTIM F AFSSSYAF ...String:
RSRKLILFIV FLALLLDNML LTVVVPIIPS YLYSIKHEKN ATEIQTARPV HTASISDSFQ SIFSYYDNST MVTGNATRDL TLHQTATQH MVTNASAVPS DCPSEDKDLL NENVQVGLLF ASKATVQLIT NPFIGLLTNR IGYPIPIFAG FCIMFVSTIM F AFSSSYAF LLIARSLQGI GSSCSSVAGM GMLASVYTDA EEAGNVMGIA LGGLAMGVLV GPPFGSVLYE FVGKTAPFLV LA ALVLLDG AIQLFVLQPS RVQPESQKGT PLTTLLKDPY ILIAAGSICF ANMGIAMLEP ALPIWMMETM CSRKWQLGVA FLP ASISYL IGTNIFGILA HKMGRWLCAL LGMIIVGVSI LCIPFAKNIY GLIAPNFGVG FAIGMVDSSM MPIMGYLVDL RHVS VYGSV YAIADVAFCM GYAIGPSAGG AIAKAIGFPW LMTIIGIIDI LFAPLCFFLR SPPQVQLVES GGALVQPGGS LRLSC AASG FPVNRYSMRW YRQAPGKERE WVAGMSSAGD RSSYEDSVKG RFTISRDDAR NTVYLQMNSL KPEDTAVYYC NVNVGF EYW GQGTQVTVSS K

UniProtKB: Synaptic vesicular amine transporter

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Macromolecule #2: Noradrenaline

MacromoleculeName: Noradrenaline / type: ligand / ID: 2 / Number of copies: 1 / Formula: E5E
Molecular weightTheoretical: 170.186 Da
Chemical component information

ChemComp-E5E:
Noradrenaline

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 6.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 60.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 176326
Initial angle assignmentType: RANDOM ASSIGNMENT
Final angle assignmentType: MAXIMUM LIKELIHOOD

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