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- EMDB-37750: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulin... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis | |||||||||
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![]() | PBS / PHOTOSYNTHESIS | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 5.1 Å | |||||||||
![]() | You X / Zhang X / Xiao YN / Sun S / Sui SF | |||||||||
Funding support | 1 items
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![]() | ![]() Title: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis Authors: You X / Zhang X / Xiao YN / Sun S / Sui SF | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 119.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 15.2 KB 15.2 KB | Display Display | ![]() |
Images | ![]() | 138.6 KB | ||
Filedesc metadata | ![]() | 3.5 KB | ||
Others | ![]() ![]() | 226.7 MB 226.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 19 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.632 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_37750_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_37750_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : In situ intact PBS-PSII supercomplex from cyanobacterial Spirulin...
Entire | Name: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis |
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Components |
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-Supramolecule #1: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulin...
Supramolecule | Name: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#21, #23-#26, #28, #27, #29-#32, #22 |
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Source (natural) | Organism: ![]() |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 35.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 1.0 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 5.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 127000 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |