+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-37720 | |||||||||
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Title | Cryo-EM structure of the human TRPC4 in lipid nanodiscs | |||||||||
Map data | ||||||||||
Sample |
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Keywords | transient receptor potential / METAL TRANSPORT | |||||||||
Function / homology | Function and homology information Role of second messengers in netrin-1 signaling / store-operated calcium channel activity / gamma-aminobutyric acid secretion / cation channel complex / inositol 1,4,5 trisphosphate binding / calcium ion import / TRP channels / cortical cytoskeleton / oligodendrocyte differentiation / regulation of cytosolic calcium ion concentration ...Role of second messengers in netrin-1 signaling / store-operated calcium channel activity / gamma-aminobutyric acid secretion / cation channel complex / inositol 1,4,5 trisphosphate binding / calcium ion import / TRP channels / cortical cytoskeleton / oligodendrocyte differentiation / regulation of cytosolic calcium ion concentration / calcium channel complex / calcium ion transmembrane transport / calcium channel activity / caveola / beta-catenin binding / calcium ion transport / basolateral plasma membrane / cadherin binding / cell surface / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.82 Å | |||||||||
Authors | Won J / Jeong H / Lee HH | |||||||||
Funding support | Korea, Republic Of, 1 items
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Citation | Journal: To Be Published Title: Structure of a TRP channel Authors: Won J / Jeong H / Lee HH | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_37720.map.gz | 117.9 MB | EMDB map data format | |
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Header (meta data) | emd-37720-v30.xml emd-37720.xml | 14.5 KB 14.5 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_37720_fsc.xml | 10.5 KB | Display | FSC data file |
Images | emd_37720.png | 143.2 KB | ||
Filedesc metadata | emd-37720.cif.gz | 5.9 KB | ||
Others | emd_37720_half_map_1.map.gz emd_37720_half_map_2.map.gz | 115.6 MB 115.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-37720 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-37720 | HTTPS FTP |
-Validation report
Summary document | emd_37720_validation.pdf.gz | 1022.9 KB | Display | EMDB validaton report |
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Full document | emd_37720_full_validation.pdf.gz | 1022.4 KB | Display | |
Data in XML | emd_37720_validation.xml.gz | 19.2 KB | Display | |
Data in CIF | emd_37720_validation.cif.gz | 24.8 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37720 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-37720 | HTTPS FTP |
-Related structure data
Related structure data | 8wpnMC 8wplC 8wpmC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_37720.map.gz / Format: CCP4 / Size: 125 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.071 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_37720_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_37720_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Structure of a TRP channel
Entire | Name: Structure of a TRP channel |
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Components |
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-Supramolecule #1: Structure of a TRP channel
Supramolecule | Name: Structure of a TRP channel / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Short transient receptor potential channel 4
Macromolecule | Name: Short transient receptor potential channel 4 / type: protein_or_peptide / ID: 1 / Details: SRASTVPRARDPPVATLEVLFQ (894-915), linker / Number of copies: 4 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 105.157977 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELL LSFNVYVGDA LLHAIRKEVV GAVELLLNHK KPSGEKQVPP ILLDKQFSEF TPDITPIILA AHTNNYEIIK L LVQKGVSV ...String: MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELL LSFNVYVGDA LLHAIRKEVV GAVELLLNHK KPSGEKQVPP ILLDKQFSEF TPDITPIILA AHTNNYEIIK L LVQKGVSV PRPHEVRCNC VECVSSSDVD SLRHSRSRLN IYKALASPSL IALSSEDPFL TAFQLSWELQ ELSKVENEFK SE YEELSRQ CKQFAKDLLD QTRSSRELEI ILNYRDDNSL IEEQSGNDLA RLKLAIKYRQ KEFVAQPNCQ QLLASRWYDE FPG WRRRHW AVKMVTCFII GLLFPVFSVC YLIAPKSPLG LFIRKPFIKF ICHTASYLTF LFLLLLASQH IDRSDLNRQG PPPT IVEWM ILPWVLGFIW GEIKQMWDGG LQDYIHDWWN LMDFVMNSLY LATISLKIVA FVKYSALNPR ESWDMWHPTL VAEAL FAIA NIFSSLRLIS LFTANSHLGP LQISLGRMLL DILKFLFIYC LVLLAFANGL NQLYFYYEET KGLTCKGIRC EKQNNA FST LFETLQSLFW SIFGLINLYV TNVKAQHEFT EFVGATMFGT YNVISLVVLL NMLIAMMNNS YQLIADHADI EWKFART KL WMSYFEEGGT LPTPFNVIPS PKSLWYLIKW IWTHLCKKKM RRKPESFGTI GRRAADNLRR HHQYQEVMRN LVKRYVAA M IRDAKTEEGL TEENFKELKQ DISSFRFEVL GLLRGSKLST IQSANASKES SNSADSDEKS DSEEEVARQQ AAGPLERNI QLESRGLASR GDLSIPGLSE QCVLVDHRER NTDTLGLQVG KRVCPFKSEK VVVEDTVPII PKEKHAKEED SSIDYDLNLP DTVTHEDYV TTRLSRASTV PRARDPPVAT LEVLFQ UniProtKB: Short transient receptor potential channel 4 |
-Macromolecule #2: CHOLESTEROL HEMISUCCINATE
Macromolecule | Name: CHOLESTEROL HEMISUCCINATE / type: ligand / ID: 2 / Number of copies: 4 / Formula: Y01 |
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Molecular weight | Theoretical: 486.726 Da |
Chemical component information | ChemComp-Y01: |
-Macromolecule #3: ZINC ION
Macromolecule | Name: ZINC ION / type: ligand / ID: 3 / Number of copies: 4 / Formula: ZN |
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Molecular weight | Theoretical: 65.409 Da |
-Macromolecule #4: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 4 / Number of copies: 4 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Macromolecule #5: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE
Macromolecule | Name: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE type: ligand / ID: 5 / Number of copies: 4 / Formula: LPP |
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Molecular weight | Theoretical: 648.891 Da |
Chemical component information | ChemComp-LPP: |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | TFS GLACIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 40.0 e/Å2 |
Electron beam | Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 1.0 µm |